The Experts below are selected from a list of 147 Experts worldwide ranked by ideXlab platform
Torsten Binscheck - One of the best experts on this subject based on the ideXlab platform.
-
Fatal poisoning with Taxus baccata. Quantification of Paclitaxel (taxol A), 10-Deacetyltaxol, Baccatin III, 10-Deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-Dimethoxyphenol in Body Fluids by Liquid Chromatography–Tandem Mass Spectrometry
2016Co-Authors: Thomas Grobosch, Bernd Schwarze, Daniel Stoecklein, Torsten BinscheckAbstract:This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based on a liquid–liquid extrac-tion under alkaline conditions followed by LC–MS–MS analysis. Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100 3 3 mm) coupled to a QTRAP 5500 system. The method allows the simultaneous identification and quantification of the yew alkaloids taxoids paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-dimethoxyphenol; the alkaloidal diterpenoids monoacetyltaxine, taxine B, monohydroxydiacetyltaxine, triacetyltaxine, and monohy-droxytriacetyltaxine were also identified. The initial hypothesis of yew tree (Taxus baccata) poisoning was confirmed. The quantita-tive evaluation revealed taxoid concentrations ranging from 4.5 to 132 mg/L (stomach content), 1 to 200 mg/L (urine), <0.5 to 12 mg/ L (cardiac blood), <0.5 to 7.3 mg/L (femoral blood), and 4.9 to 290 mg/L (bile). In brain tissue, none of these taxoids could be detected (<0.5 mg/L). In urine, after enzymatic hydrolysis, the con-centration of 3,5-dimethoxyphenol (3,5-DMP) was 23,000 mg/L. The alkaloidal diterpenoids were found in all postmortem samples. The newly developed LC–MS–MS method enables the identifica-tion of alkaloidal and non-alkaloidal diterpenoids and 3,5-dimethox-yphenol in human body fluids and tissues for the confirmation of accidental or intentional poisonings with yew plant material
-
eight cases of fatal and non fatal poisoning with taxus baccata
Forensic Science International, 2013Co-Authors: Thomas Grobosch, Bernd Schwarze, Norbert Felgenhauer, Benno Riesselmann, Sonja Roscher, Torsten BinscheckAbstract:This paper describes two fatalities, three non-fatal intentional and three accidental oral ingestions of yew (Taxus baccata) leaves. In all cases the post-mortem external examinations showed no signs of violence. Internal examinations revealed small green, needle-like particles on the tongue, in the esophagus and in the stomach. Yew leaves were also identified in the stomach contents, whereas Taxus leaves were cut into small pieces and then ingested in one case. The analytical method used was based on a liquid-liquid-extraction under alkaline conditions followed by LC-MS/MS analysis (QTRAP 5500). Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100×3) mm. The analytical method allows the simultaneous identification and quantification of the commercially available yew alkaloids taxoids (m/z): paclitaxel (854.2→105.0/286.1), 10-deacetyltaxol (10-DAT: 812.2→105.0/286.1), baccatin III (BAC III: 604.0→105.0/327.0), 10-deacetylbaccatin III (10-DAB III: 562.1→105.0/327.0), Cephalomannine [taxol B] (562.1→105.0/327.0) and of 3,5-dimethoxyphenol (3,5-DMP: 155.0→111.9/122.9) also encompassing the qualitative analysis of the alkaloidal diterpenoids (Q1→194.0/107.0); reference mass spectra obtained from a yew leaves extract: monoacetyltaxine (MAT: 568.4), taxine B (584.2), monohydroxydiacetyltaxine (MHDAT: 626.4), triacetyltaxine (TAT: 652.4), monohydroxytriacetyltaxine (MHTAT: 668.4). In both fatalities, paclitaxel, 10-DAT and Cephalomannine were not identified in urine, cardiac and femoral blood but all taxoids and 3,5-DMP were present in stomach content and excreted into the bile. In urine, highest 3,5-DMP concentration was 7500 μg/L and 23,000 μg/L after enzymatic hydrolysis, respectively. In intentional and accidental poisonings, when electrocardiogram (ECG) examinations revealed ventricular tachycardia and/or prolonged QRS intervals, taxines were identified in plasma/serum, even after the ingestion of a few number of yew leaves, when 3,5-dimethoxyphenol was not even found. According to the data from one near-fatal intentional poisoning, elimination half-life of MAT, TAXIN B, MHDAT and MHTAT in serum was calculated with 11-13 h and taxines were detected up to t=+122 h post-ingestion of approximately two handfuls of yew leaves.
-
Fatal poisoning with Taxus baccata. Quantification of Paclitaxel (taxol A), 10-Deacetyltaxol, Baccatin III, 10-Deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-Dimethoxyphenol in Body Fluids by Liquid Chromatography–Tandem Mass Spectrometry
Journal of Analytical Toxicology, 2012Co-Authors: Thomas Grobosch, Bernd Schwarze, Daniel Stoecklein, Torsten BinscheckAbstract:This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based on a liquid-liquid extraction under alkaline conditions followed by LC-MS-MS analysis. Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100 × 3 mm) coupled to a QTRAP 5500 system. The method allows the simultaneous identification and quantification of the yew alkaloids taxoids paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-dimethoxyphenol; the alkaloidal diterpenoids monoacetyltaxine, taxine B, monohydroxydiacetyltaxine, triacetyltaxine, and monohydroxytriacetyltaxine were also identified. The initial hypothesis of yew tree (Taxus baccata) poisoning was confirmed. The quantitative evaluation revealed taxoid concentrations ranging from 4.5 to 132 µg/L (stomach content), 1 to 200 µg/L (urine), Language: en
Thomas Grobosch - One of the best experts on this subject based on the ideXlab platform.
-
Fatal poisoning with Taxus baccata. Quantification of Paclitaxel (taxol A), 10-Deacetyltaxol, Baccatin III, 10-Deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-Dimethoxyphenol in Body Fluids by Liquid Chromatography–Tandem Mass Spectrometry
2016Co-Authors: Thomas Grobosch, Bernd Schwarze, Daniel Stoecklein, Torsten BinscheckAbstract:This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based on a liquid–liquid extrac-tion under alkaline conditions followed by LC–MS–MS analysis. Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100 3 3 mm) coupled to a QTRAP 5500 system. The method allows the simultaneous identification and quantification of the yew alkaloids taxoids paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-dimethoxyphenol; the alkaloidal diterpenoids monoacetyltaxine, taxine B, monohydroxydiacetyltaxine, triacetyltaxine, and monohy-droxytriacetyltaxine were also identified. The initial hypothesis of yew tree (Taxus baccata) poisoning was confirmed. The quantita-tive evaluation revealed taxoid concentrations ranging from 4.5 to 132 mg/L (stomach content), 1 to 200 mg/L (urine), <0.5 to 12 mg/ L (cardiac blood), <0.5 to 7.3 mg/L (femoral blood), and 4.9 to 290 mg/L (bile). In brain tissue, none of these taxoids could be detected (<0.5 mg/L). In urine, after enzymatic hydrolysis, the con-centration of 3,5-dimethoxyphenol (3,5-DMP) was 23,000 mg/L. The alkaloidal diterpenoids were found in all postmortem samples. The newly developed LC–MS–MS method enables the identifica-tion of alkaloidal and non-alkaloidal diterpenoids and 3,5-dimethox-yphenol in human body fluids and tissues for the confirmation of accidental or intentional poisonings with yew plant material
-
eight cases of fatal and non fatal poisoning with taxus baccata
Forensic Science International, 2013Co-Authors: Thomas Grobosch, Bernd Schwarze, Norbert Felgenhauer, Benno Riesselmann, Sonja Roscher, Torsten BinscheckAbstract:This paper describes two fatalities, three non-fatal intentional and three accidental oral ingestions of yew (Taxus baccata) leaves. In all cases the post-mortem external examinations showed no signs of violence. Internal examinations revealed small green, needle-like particles on the tongue, in the esophagus and in the stomach. Yew leaves were also identified in the stomach contents, whereas Taxus leaves were cut into small pieces and then ingested in one case. The analytical method used was based on a liquid-liquid-extraction under alkaline conditions followed by LC-MS/MS analysis (QTRAP 5500). Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100×3) mm. The analytical method allows the simultaneous identification and quantification of the commercially available yew alkaloids taxoids (m/z): paclitaxel (854.2→105.0/286.1), 10-deacetyltaxol (10-DAT: 812.2→105.0/286.1), baccatin III (BAC III: 604.0→105.0/327.0), 10-deacetylbaccatin III (10-DAB III: 562.1→105.0/327.0), Cephalomannine [taxol B] (562.1→105.0/327.0) and of 3,5-dimethoxyphenol (3,5-DMP: 155.0→111.9/122.9) also encompassing the qualitative analysis of the alkaloidal diterpenoids (Q1→194.0/107.0); reference mass spectra obtained from a yew leaves extract: monoacetyltaxine (MAT: 568.4), taxine B (584.2), monohydroxydiacetyltaxine (MHDAT: 626.4), triacetyltaxine (TAT: 652.4), monohydroxytriacetyltaxine (MHTAT: 668.4). In both fatalities, paclitaxel, 10-DAT and Cephalomannine were not identified in urine, cardiac and femoral blood but all taxoids and 3,5-DMP were present in stomach content and excreted into the bile. In urine, highest 3,5-DMP concentration was 7500 μg/L and 23,000 μg/L after enzymatic hydrolysis, respectively. In intentional and accidental poisonings, when electrocardiogram (ECG) examinations revealed ventricular tachycardia and/or prolonged QRS intervals, taxines were identified in plasma/serum, even after the ingestion of a few number of yew leaves, when 3,5-dimethoxyphenol was not even found. According to the data from one near-fatal intentional poisoning, elimination half-life of MAT, TAXIN B, MHDAT and MHTAT in serum was calculated with 11-13 h and taxines were detected up to t=+122 h post-ingestion of approximately two handfuls of yew leaves.
-
Fatal poisoning with Taxus baccata. Quantification of Paclitaxel (taxol A), 10-Deacetyltaxol, Baccatin III, 10-Deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-Dimethoxyphenol in Body Fluids by Liquid Chromatography–Tandem Mass Spectrometry
Journal of Analytical Toxicology, 2012Co-Authors: Thomas Grobosch, Bernd Schwarze, Daniel Stoecklein, Torsten BinscheckAbstract:This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based on a liquid-liquid extraction under alkaline conditions followed by LC-MS-MS analysis. Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100 × 3 mm) coupled to a QTRAP 5500 system. The method allows the simultaneous identification and quantification of the yew alkaloids taxoids paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-dimethoxyphenol; the alkaloidal diterpenoids monoacetyltaxine, taxine B, monohydroxydiacetyltaxine, triacetyltaxine, and monohydroxytriacetyltaxine were also identified. The initial hypothesis of yew tree (Taxus baccata) poisoning was confirmed. The quantitative evaluation revealed taxoid concentrations ranging from 4.5 to 132 µg/L (stomach content), 1 to 200 µg/L (urine), Language: en
Hongwei Luan - One of the best experts on this subject based on the ideXlab platform.
-
profiling of yew hair roots from various species using ultra performance liquid chromatography electrospray ionization mass spectrometry
Rapid Communications in Mass Spectrometry, 2008Co-Authors: Hongwei Luan, Yanyan Zhang, Xingbao Liu, Li Yang, Zhengtao Wang, Ling YangAbstract:An efficient and sensitive profiling approach to complex yew samples was developed using ultra-performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS). The UPLC-based method displayed short analytical time and improved peak capability, as well as high sensitivity. The appropriate in-source collision-induced dissociation (CID) energy was employed to produce informative characteristic ions which could be used for stereochemical and sub-structural assignment of yew constituents. The method was successfully applied in the rapid screening of yew hair roots from various species, and 53 constituents including 47 taxoids were detected from partially purified root extract. Notably, C-7 hydroxytaxane stereoisomers could be identified based on their different fragment ions under the optimal profiling conditions. It was also observed that hair roots from different Taxus species exhibited nearly identical chemical distribution, indicating they had similar metabolic frameworks. Additionally, Taxus root resources also display benign medicinal perspective because they have relatively simple chemical profiles and possess high yields of valuable taxanes such as paclitaxel, Cephalomannine, 10-deacetylpaclitaxel and 7-xylosyltaxanes.
Ling Yang - One of the best experts on this subject based on the ideXlab platform.
-
Taxus metabolomic study and the elucidation of the taxane biosynthetic framework in Taxus root
2009Co-Authors: Ling YangAbstract:The medicinal plant Taxus (Taxaceae), an important source of a series of biological active compounds including anticancer agent paclitaxel and its analogs, has drawn much attention and been under intense investigation during the past fifty years. Paclitaxel and its semi-synthetic precursors belong to a group of typical secondary metabolites named the taxane diterpenoids or taxoids, but their distribution and level are very variable with species, tissue, and growing conditions. In order to explore the differences in accumulation and in distribution of metabolites in various Taxus species, an efficient and sensitive profiling approach to complex yew samples was developed using ultra-performance liquid chromatography-electrospray ionization mass spectrometry (UPLC-ESI-MS) in this study. The appropriate in-source CID energy was employed to produce informative characteristic ions which could be used for stereochemical and sub-structural assignment of yew constituents. The method was successfully applied in rapid screening of Taxus needle and root samples from various species including T. yunnanensis, T. chinensis, T. mairei, T. × media, T. cuspidata, T. wallichiana, and T. canadensis. Most yew constituents can be characterized based on the fragment behavior of model compounds, and fifty-three constituents including forty-seven taxoids were detected from Taxus root extract. It is evident from the metabolic profiling results that root samples from different Taxus species have similar profile and nearly identical chemical distribution, which suggests that Taxus roots have similar metabolic frameworks. In sharp contrast, needle extracts from various Taxus species displayed significant metabolic variations both in chemical distribution and in concentration. It is also very impressing that thirty-three taxoids with 10-DAB skeleton existed in Taxus root, which can be used as semi-synthetic precursors for paclitaxel. These taxoids are also recognized as intermediates or products involved in parallel pathways of paclitaxel formation via the important intermediate 10-DAB during taxane biosynthesis. On the basis of the appearance and connectivity of all detected taxanes in Taxus root, the down-stream biosynthetic framework in Taxus roots was explored in combination of related information on taxane biosynthesis. Additionally, Taxus root resources also display benign medicinal perspective, due to the high yield of valuable taxanes including paclitaxel, Cephalomannine, 10-deacetylpaclitaxel and 7-xylosyltaxanes in this tissue. The proposed UPLC-based profiling method is suitable for large-scale screening of crude yew samples and can be applied in quality control and metabolic study of this uncommon plant. This study also can promote further studies on plant metabolomics and on sustainable utilization of Taxus resources
-
profiling of yew hair roots from various species using ultra performance liquid chromatography electrospray ionization mass spectrometry
Rapid Communications in Mass Spectrometry, 2008Co-Authors: Hongwei Luan, Yanyan Zhang, Xingbao Liu, Li Yang, Zhengtao Wang, Ling YangAbstract:An efficient and sensitive profiling approach to complex yew samples was developed using ultra-performance liquid chromatography/electrospray ionization mass spectrometry (UPLC/ESI-MS). The UPLC-based method displayed short analytical time and improved peak capability, as well as high sensitivity. The appropriate in-source collision-induced dissociation (CID) energy was employed to produce informative characteristic ions which could be used for stereochemical and sub-structural assignment of yew constituents. The method was successfully applied in the rapid screening of yew hair roots from various species, and 53 constituents including 47 taxoids were detected from partially purified root extract. Notably, C-7 hydroxytaxane stereoisomers could be identified based on their different fragment ions under the optimal profiling conditions. It was also observed that hair roots from different Taxus species exhibited nearly identical chemical distribution, indicating they had similar metabolic frameworks. Additionally, Taxus root resources also display benign medicinal perspective because they have relatively simple chemical profiles and possess high yields of valuable taxanes such as paclitaxel, Cephalomannine, 10-deacetylpaclitaxel and 7-xylosyltaxanes.
Bernd Schwarze - One of the best experts on this subject based on the ideXlab platform.
-
Fatal poisoning with Taxus baccata. Quantification of Paclitaxel (taxol A), 10-Deacetyltaxol, Baccatin III, 10-Deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-Dimethoxyphenol in Body Fluids by Liquid Chromatography–Tandem Mass Spectrometry
2016Co-Authors: Thomas Grobosch, Bernd Schwarze, Daniel Stoecklein, Torsten BinscheckAbstract:This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based on a liquid–liquid extrac-tion under alkaline conditions followed by LC–MS–MS analysis. Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100 3 3 mm) coupled to a QTRAP 5500 system. The method allows the simultaneous identification and quantification of the yew alkaloids taxoids paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-dimethoxyphenol; the alkaloidal diterpenoids monoacetyltaxine, taxine B, monohydroxydiacetyltaxine, triacetyltaxine, and monohy-droxytriacetyltaxine were also identified. The initial hypothesis of yew tree (Taxus baccata) poisoning was confirmed. The quantita-tive evaluation revealed taxoid concentrations ranging from 4.5 to 132 mg/L (stomach content), 1 to 200 mg/L (urine), <0.5 to 12 mg/ L (cardiac blood), <0.5 to 7.3 mg/L (femoral blood), and 4.9 to 290 mg/L (bile). In brain tissue, none of these taxoids could be detected (<0.5 mg/L). In urine, after enzymatic hydrolysis, the con-centration of 3,5-dimethoxyphenol (3,5-DMP) was 23,000 mg/L. The alkaloidal diterpenoids were found in all postmortem samples. The newly developed LC–MS–MS method enables the identifica-tion of alkaloidal and non-alkaloidal diterpenoids and 3,5-dimethox-yphenol in human body fluids and tissues for the confirmation of accidental or intentional poisonings with yew plant material
-
eight cases of fatal and non fatal poisoning with taxus baccata
Forensic Science International, 2013Co-Authors: Thomas Grobosch, Bernd Schwarze, Norbert Felgenhauer, Benno Riesselmann, Sonja Roscher, Torsten BinscheckAbstract:This paper describes two fatalities, three non-fatal intentional and three accidental oral ingestions of yew (Taxus baccata) leaves. In all cases the post-mortem external examinations showed no signs of violence. Internal examinations revealed small green, needle-like particles on the tongue, in the esophagus and in the stomach. Yew leaves were also identified in the stomach contents, whereas Taxus leaves were cut into small pieces and then ingested in one case. The analytical method used was based on a liquid-liquid-extraction under alkaline conditions followed by LC-MS/MS analysis (QTRAP 5500). Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100×3) mm. The analytical method allows the simultaneous identification and quantification of the commercially available yew alkaloids taxoids (m/z): paclitaxel (854.2→105.0/286.1), 10-deacetyltaxol (10-DAT: 812.2→105.0/286.1), baccatin III (BAC III: 604.0→105.0/327.0), 10-deacetylbaccatin III (10-DAB III: 562.1→105.0/327.0), Cephalomannine [taxol B] (562.1→105.0/327.0) and of 3,5-dimethoxyphenol (3,5-DMP: 155.0→111.9/122.9) also encompassing the qualitative analysis of the alkaloidal diterpenoids (Q1→194.0/107.0); reference mass spectra obtained from a yew leaves extract: monoacetyltaxine (MAT: 568.4), taxine B (584.2), monohydroxydiacetyltaxine (MHDAT: 626.4), triacetyltaxine (TAT: 652.4), monohydroxytriacetyltaxine (MHTAT: 668.4). In both fatalities, paclitaxel, 10-DAT and Cephalomannine were not identified in urine, cardiac and femoral blood but all taxoids and 3,5-DMP were present in stomach content and excreted into the bile. In urine, highest 3,5-DMP concentration was 7500 μg/L and 23,000 μg/L after enzymatic hydrolysis, respectively. In intentional and accidental poisonings, when electrocardiogram (ECG) examinations revealed ventricular tachycardia and/or prolonged QRS intervals, taxines were identified in plasma/serum, even after the ingestion of a few number of yew leaves, when 3,5-dimethoxyphenol was not even found. According to the data from one near-fatal intentional poisoning, elimination half-life of MAT, TAXIN B, MHDAT and MHTAT in serum was calculated with 11-13 h and taxines were detected up to t=+122 h post-ingestion of approximately two handfuls of yew leaves.
-
Fatal poisoning with Taxus baccata. Quantification of Paclitaxel (taxol A), 10-Deacetyltaxol, Baccatin III, 10-Deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-Dimethoxyphenol in Body Fluids by Liquid Chromatography–Tandem Mass Spectrometry
Journal of Analytical Toxicology, 2012Co-Authors: Thomas Grobosch, Bernd Schwarze, Daniel Stoecklein, Torsten BinscheckAbstract:This method development was to confirm the fatal ingestion of toxic yew plant material in postmortem samples (stomach content, urine, femoral blood, cardiac blood, bile, and brain tissue) collected from a 22-year-old man who committed suicide by ingesting yew leaves. The analytical method was based on a liquid-liquid extraction under alkaline conditions followed by LC-MS-MS analysis. Chromatographic separation was achieved by HPLC on a Kinetex C18 2.6u (100 × 3 mm) coupled to a QTRAP 5500 system. The method allows the simultaneous identification and quantification of the yew alkaloids taxoids paclitaxel (taxol A), 10-deacetyltaxol, baccatin III, 10-deacetylbaccatin III, Cephalomannine (taxol B), and 3,5-dimethoxyphenol; the alkaloidal diterpenoids monoacetyltaxine, taxine B, monohydroxydiacetyltaxine, triacetyltaxine, and monohydroxytriacetyltaxine were also identified. The initial hypothesis of yew tree (Taxus baccata) poisoning was confirmed. The quantitative evaluation revealed taxoid concentrations ranging from 4.5 to 132 µg/L (stomach content), 1 to 200 µg/L (urine), Language: en