The Experts below are selected from a list of 6300 Experts worldwide ranked by ideXlab platform
Atsushi Matsuzawa - One of the best experts on this subject based on the ideXlab platform.
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nuclear accumulated sqstm1 p62 based alis act as microdomains sensing cellular stresses and triggering oxidative stress induced parthanatos
Cell Death and Disease, 2018Co-Authors: Takuya Noguchi, Midori Suzuki, Natsumi Mutoh, Yusuke Hirata, Mei Tsuchida, Sayoko Miyagawa, Gi-wook Hwang, Junken Aoki, Atsushi MatsuzawaAbstract:Aggresome-like induced structures (ALIS) have been described as ubiquitinated protein-containing aggresomes transiently formed in response to various stresses. In this study, we provide evidence that ALIS composed of SQSTM1/p62 act as a key determinant of oxidative stress-induced parthanatos, which is newly discovered and distinct from regular programmed cell death. Interestingly, we first found that chemical stresses induced by particular chemical drugs, such as several Cephalosporin Antibiotics, cause oxidative stress-mediated parthanatos, accompanied by the ALIS formation. Blocking the ALIS formation potently suppressed the parthanatos, and p62 knockout cells exhibited the attenuated ALIS formation and high resistance to parthanatos. Moreover, we also found that the redox-sensing activity of p62 is required for nuclear accumulation of the p62-based ALIS, resulting in the induction of parthanatos. Together, our results demonstrate unexpected functions of p62 and ALIS as cell death mediators sensing oxidative stress, and thus uncover a novel mechanism whereby p62 mediates parthanatos.
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Nuclear-accumulated SQSTM1/p62-based ALIS act as microdomains sensing cellular stresses and triggering oxidative stress-induced parthanatos
Nature Publishing Group, 2018Co-Authors: Takuya Noguchi, Midori Suzuki, Natsumi Mutoh, Yusuke Hirata, Mei Tsuchida, Sayoko Miyagawa, Gi-wook Hwang, Junken Aoki, Atsushi MatsuzawaAbstract:Abstract Aggresome-like induced structures (ALIS) have been described as ubiquitinated protein-containing aggresomes transiently formed in response to various stresses. In this study, we provide evidence that ALIS composed of SQSTM1/p62 act as a key determinant of oxidative stress-induced parthanatos, which is newly discovered and distinct from regular programmed cell death. Interestingly, we first found that chemical stresses induced by particular chemical drugs, such as several Cephalosporin Antibiotics, cause oxidative stress-mediated parthanatos, accompanied by the ALIS formation. Blocking the ALIS formation potently suppressed the parthanatos, and p62 knockout cells exhibited the attenuated ALIS formation and high resistance to parthanatos. Moreover, we also found that the redox-sensing activity of p62 is required for nuclear accumulation of the p62-based ALIS, resulting in the induction of parthanatos. Together, our results demonstrate unexpected functions of p62 and ALIS as cell death mediators sensing oxidative stress, and thus uncover a novel mechanism whereby p62 mediates parthanatos
David L Paterson - One of the best experts on this subject based on the ideXlab platform.
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outcome of Cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended spectrum β lactamases implications for the clinical microbiology laboratory
Journal of Clinical Microbiology, 2001Co-Authors: Keith P Klugman, David L Paterson, Anne Von Gottberg, Jose Maria Casellas, Lutfiye Mulazimoglu, Robert A Bonomo, Louis B Rice, J G MccormackAbstract:Although extended-spectrum beta-lactamases (ESBLs) hydrolyze Cephalosporin Antibiotics, some ESBL-producing organisms are not resistant to all Cephalosporins when tested in vitro. Some authors have suggested that screening klebsiellae or Escherichia coli for ESBL production is not clinically necessary, and when most recently surveyed the majority of American clinical microbiology laboratories did not make efforts to detect ESBLs. We performed a prospective, multinational study of Klebsiella pneumoniae bacteremia and identified 10 patients who were treated for ESBL-producing K. pneumoniae bacteremia with Cephalosporins and whose infecting organisms were not resistant in vitro to the utilized Cephalosporin. In addition, we reviewed 26 similar cases of severe infections which had previously been reported. Of these 36 patients, 4 had to be excluded from analysis. Of the remaining 32 patients, 100% (4 of 4) patients experienced clinical failure when MICs of the Cephalosporin used for treatment were in the intermediate range and 54% (15 of 28) experienced failure when MICs of the Cephalosporin used for treatment were in the susceptible range. Thus, it is clinically important to detect ESBL production by klebsiellae or E. coli even when Cephalosporin MICs are in the susceptible range (≤ 8 μg/ml) and to report ESBL-producing organisms as resistant to aztreonam and all Cephalosporins (with the exception of cephamycins).
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outcome of Cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended spectrum beta lactamases implications for the clinical microbiology laboratory
Journal of Clinical Microbiology, 2001Co-Authors: Keith P Klugman, David L Paterson, Anne Von Gottberg, Jose Maria Casellas, Lutfiye Mulazimoglu, Robert A Bonomo, Louis B Rice, J G MccormackAbstract:Although extended-spectrum beta-lactamases (ESBLs) hydrolyze Cephalosporin Antibiotics, some ESBL-producing organisms are not resistant to all Cephalosporins when tested in vitro. Some authors have suggested that screening klebsiellae or Escherichia coli for ESBL production is not clinically necessary, and when most recently surveyed the majority of American clinical microbiology laboratories did not make efforts to detect ESBLs. We performed a prospective, multinational study of Klebsiella pneumoniae bacteremia and identified 10 patients who were treated for ESBL-producing K. pneumoniae bacteremia with Cephalosporins and whose infecting organisms were not resistant in vitro to the utilized Cephalosporin. In addition, we reviewed 26 similar cases of severe infections which had previously been reported. Of these 36 patients, 4 had to be excluded from analysis. Of the remaining 32 patients, 100% (4 of 4) patients experienced clinical failure when MICs of the Cephalosporin used for treatment were in the intermediate range and 54% (15 of 28) experienced failure when MICs of the Cephalosporin used for treatment were in the susceptible range. Thus, it is clinically important to detect ESBL production by klebsiellae or E. coli even when Cephalosporin MICs are in the susceptible range (≤ 8 μg/ml) and to report ESBL-producing organisms as resistant to aztreonam and all Cephalosporins (with the exception of cephamycins).
Takuya Noguchi - One of the best experts on this subject based on the ideXlab platform.
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nuclear accumulated sqstm1 p62 based alis act as microdomains sensing cellular stresses and triggering oxidative stress induced parthanatos
Cell Death and Disease, 2018Co-Authors: Takuya Noguchi, Midori Suzuki, Natsumi Mutoh, Yusuke Hirata, Mei Tsuchida, Sayoko Miyagawa, Gi-wook Hwang, Junken Aoki, Atsushi MatsuzawaAbstract:Aggresome-like induced structures (ALIS) have been described as ubiquitinated protein-containing aggresomes transiently formed in response to various stresses. In this study, we provide evidence that ALIS composed of SQSTM1/p62 act as a key determinant of oxidative stress-induced parthanatos, which is newly discovered and distinct from regular programmed cell death. Interestingly, we first found that chemical stresses induced by particular chemical drugs, such as several Cephalosporin Antibiotics, cause oxidative stress-mediated parthanatos, accompanied by the ALIS formation. Blocking the ALIS formation potently suppressed the parthanatos, and p62 knockout cells exhibited the attenuated ALIS formation and high resistance to parthanatos. Moreover, we also found that the redox-sensing activity of p62 is required for nuclear accumulation of the p62-based ALIS, resulting in the induction of parthanatos. Together, our results demonstrate unexpected functions of p62 and ALIS as cell death mediators sensing oxidative stress, and thus uncover a novel mechanism whereby p62 mediates parthanatos.
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Nuclear-accumulated SQSTM1/p62-based ALIS act as microdomains sensing cellular stresses and triggering oxidative stress-induced parthanatos
Nature Publishing Group, 2018Co-Authors: Takuya Noguchi, Midori Suzuki, Natsumi Mutoh, Yusuke Hirata, Mei Tsuchida, Sayoko Miyagawa, Gi-wook Hwang, Junken Aoki, Atsushi MatsuzawaAbstract:Abstract Aggresome-like induced structures (ALIS) have been described as ubiquitinated protein-containing aggresomes transiently formed in response to various stresses. In this study, we provide evidence that ALIS composed of SQSTM1/p62 act as a key determinant of oxidative stress-induced parthanatos, which is newly discovered and distinct from regular programmed cell death. Interestingly, we first found that chemical stresses induced by particular chemical drugs, such as several Cephalosporin Antibiotics, cause oxidative stress-mediated parthanatos, accompanied by the ALIS formation. Blocking the ALIS formation potently suppressed the parthanatos, and p62 knockout cells exhibited the attenuated ALIS formation and high resistance to parthanatos. Moreover, we also found that the redox-sensing activity of p62 is required for nuclear accumulation of the p62-based ALIS, resulting in the induction of parthanatos. Together, our results demonstrate unexpected functions of p62 and ALIS as cell death mediators sensing oxidative stress, and thus uncover a novel mechanism whereby p62 mediates parthanatos
J G Mccormack - One of the best experts on this subject based on the ideXlab platform.
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outcome of Cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended spectrum β lactamases implications for the clinical microbiology laboratory
Journal of Clinical Microbiology, 2001Co-Authors: Keith P Klugman, David L Paterson, Anne Von Gottberg, Jose Maria Casellas, Lutfiye Mulazimoglu, Robert A Bonomo, Louis B Rice, J G MccormackAbstract:Although extended-spectrum beta-lactamases (ESBLs) hydrolyze Cephalosporin Antibiotics, some ESBL-producing organisms are not resistant to all Cephalosporins when tested in vitro. Some authors have suggested that screening klebsiellae or Escherichia coli for ESBL production is not clinically necessary, and when most recently surveyed the majority of American clinical microbiology laboratories did not make efforts to detect ESBLs. We performed a prospective, multinational study of Klebsiella pneumoniae bacteremia and identified 10 patients who were treated for ESBL-producing K. pneumoniae bacteremia with Cephalosporins and whose infecting organisms were not resistant in vitro to the utilized Cephalosporin. In addition, we reviewed 26 similar cases of severe infections which had previously been reported. Of these 36 patients, 4 had to be excluded from analysis. Of the remaining 32 patients, 100% (4 of 4) patients experienced clinical failure when MICs of the Cephalosporin used for treatment were in the intermediate range and 54% (15 of 28) experienced failure when MICs of the Cephalosporin used for treatment were in the susceptible range. Thus, it is clinically important to detect ESBL production by klebsiellae or E. coli even when Cephalosporin MICs are in the susceptible range (≤ 8 μg/ml) and to report ESBL-producing organisms as resistant to aztreonam and all Cephalosporins (with the exception of cephamycins).
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outcome of Cephalosporin treatment for serious infections due to apparently susceptible organisms producing extended spectrum beta lactamases implications for the clinical microbiology laboratory
Journal of Clinical Microbiology, 2001Co-Authors: Keith P Klugman, David L Paterson, Anne Von Gottberg, Jose Maria Casellas, Lutfiye Mulazimoglu, Robert A Bonomo, Louis B Rice, J G MccormackAbstract:Although extended-spectrum beta-lactamases (ESBLs) hydrolyze Cephalosporin Antibiotics, some ESBL-producing organisms are not resistant to all Cephalosporins when tested in vitro. Some authors have suggested that screening klebsiellae or Escherichia coli for ESBL production is not clinically necessary, and when most recently surveyed the majority of American clinical microbiology laboratories did not make efforts to detect ESBLs. We performed a prospective, multinational study of Klebsiella pneumoniae bacteremia and identified 10 patients who were treated for ESBL-producing K. pneumoniae bacteremia with Cephalosporins and whose infecting organisms were not resistant in vitro to the utilized Cephalosporin. In addition, we reviewed 26 similar cases of severe infections which had previously been reported. Of these 36 patients, 4 had to be excluded from analysis. Of the remaining 32 patients, 100% (4 of 4) patients experienced clinical failure when MICs of the Cephalosporin used for treatment were in the intermediate range and 54% (15 of 28) experienced failure when MICs of the Cephalosporin used for treatment were in the susceptible range. Thus, it is clinically important to detect ESBL production by klebsiellae or E. coli even when Cephalosporin MICs are in the susceptible range (≤ 8 μg/ml) and to report ESBL-producing organisms as resistant to aztreonam and all Cephalosporins (with the exception of cephamycins).
Jo Shu Chang - One of the best experts on this subject based on the ideXlab platform.
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removal of Cephalosporin Antibiotics 7 aca from wastewater during the cultivation of lipid accumulating microalgae
Bioresource Technology, 2016Co-Authors: Wan Qian Guo, He Shan Zheng, Xiao Chi Feng, Renli Yin, Nanqi Ren, Jo Shu ChangAbstract:The aim of this study is to evaluate the feasibility of using lipid-accumulating microalgae to remove Cephalosporin Antibiotics 7-amino cephalosporanic acid (7-ACA) from wastewater with the additional benefit of biofuels production. Three isolated microalgal strains (namely, Chlorella sp. Cha-01, Chlamydomonas sp. Tai-03 and Mychonastes sp. YL-02) were cultivated under 7-ACA stress and their biomass productivity, lipid production and N-NO3- consumption were monitored. It was found that 7-ACA had slight inhibition effects on the microalgal growth at the ratio of 12.0% (Cha-01), 9.6% (YL-02), 11.7% (Tai-03). However, lipid accumulation in the three microalgae was not influenced by the presence of 7-ACA. The investigation on the 7-ACA removal mechanisms during microalgal growth shows that 7-ACA was mainly removed by microalgae adsorption as well as hydrolysis and photolysis reactions. This study demonstrates that using microalgae to treat antibiotic-containing wastewater is promising due to the potential of simultaneous antibiotic removal and biofuel production.