The Experts below are selected from a list of 1482 Experts worldwide ranked by ideXlab platform

R. F. Eastwood - One of the best experts on this subject based on the ideXlab platform.

  • impact of cre1 cre8 and cre3 genes on Cereal Cyst Nematode resistance in wheat
    Theoretical and Applied Genetics, 2005
    Co-Authors: E Safari, R. F. Eastwood, John Lewis, H. A. Eagles, N N Gororo, Francis C. Ogbonnaya
    Abstract:

    The Cereal Cyst Nematode (CCN; Heterodera avenae), a root disease of Cereal crops, is a major economic constraint in many wheat (Triticum aestivum)-growing areas of the world. The objective of this study was to assess the impact of the Cre1, Cre8 and Cre3 genes on CCN resistance. A population of 92 doubled-haploid (DH) lines derived from a cross between wheat cvs. Frame and Silverstar as well as 1,851 wheat breeding lines were screened for CCN resistance at the Primary Industries Research Victoria (PIRVic). A second population of 9,470 wheat breeding lines was screened at the South Australian Research and Development Institute (SARDI). Cre3 had the largest impact on reducing the number of female Cysts, followed by Cre1 and Cre8. There was no significant difference in number of Cysts between DH lines with or without the Cre8 marker, suggesting that the marker is not perfectly linked to Cre8. The estimated heritabilities were 0.32 in the DH population, 0.48 in the PIRVic data set and 0.32 in the SARDI data set, which confirm that this is a trait of low heritability. The repeatability of CCN resistance improved with an increase in the number of plants assessed per line—up to ten. However, 85–88% of the improvement was achieved with the assessments of the first five plants.

  • Diagnostic DNA markers for Cereal Cyst Nematode resistance in bread wheat
    Australian Journal of Agricultural Research, 2001
    Co-Authors: Francis C. Ogbonnaya, R. F. Eastwood, Rudi Appels, Jf Kollmorgen, N. C. Subrahmanyam, O. Moullet, J. De Majnik, H. A. Eagles, J. S. Brown, Evans S. Lagudah
    Abstract:

    The development of cultivars resistant to Cereal Cyst Nematode (CCN) is a primary objective in wheat breeding in the southern wheatbelt of Australia. Nine CCN resistance genes have been identified in wheat and its relatives, some of which confer resistance to the Australian pathotype of CCN (Ha13). Cultivars released in Australia with CCN resistance carry either the Cre1 or CreF gene, with the Cre3 gene present in advanced breeding lines. The biological assay for CCN resistance screening in wheat is time-consuming, not reliable on a single-plant basis, and prone to inconsistencies, thus reducing the efficiency of selection amongst breeding lines. Using gene sequences initially isolated from the Cre3 locus, a DNA-based marker selection system was developed and applied to unambiguously identify wheat lines carrying resistance alleles at theCre1 and/or Cre3 loci in breeding populations derived from diverse genetic backgrounds. Homologues of sequences from the Cre3 locus, located elsewhere in the wheat genome, can also be used to select wheat lines with a newly identified CCN resistance gene (Cre6) introgressed from Aegilops ventricosa. Application of these markers has become an integral part of the southern Australian breeding programs.

  • Triticum tauschii: a novel source of resistance to Cereal Cyst Nematode (Heterodera avenae)
    Australian Journal of Agricultural Research, 1991
    Co-Authors: R. F. Eastwood, Evans S. Lagudah, Rudi Appels, M Hannah, Jf Kollmorgen
    Abstract:

    Four hundred and twenty accessions of Triticum tauschii (genome DD) were assessed for resistance to Cereal Cyst Nematode (Heterodera avenae Woll.) and 13 were found to be resistant (P = 0.05). Synthetic allohexaploid wheats produced from different Triticum turgidurn var. durum (genome AABB) lines and H. avenae resistant accessions of T tauschii maintained the level of resistance expressed in the diploids. This is the first report of resistance to H. avenae in T. tauschii, and in synthesized allohexaploid wheat. Since the resistance is in the D genome it is likely to be novel. Resistance was most common in T. tauschii ssp. eusquarrosa var. meyeri, and its derivatives which are confined to the Pahlavi region of Iran.

  • Corrigendum - Triticum tauschii: a novel source of resistance to Cereal Cyst Nematode (Heterodera avenae)
    Australian Journal of Agricultural Research, 1991
    Co-Authors: R. F. Eastwood, Evans S. Lagudah, Rudi Appels, M Hannah, Jf Kollmorgen
    Abstract:

    Four hundred and twenty accessions of Triticum tauschii (genome DD) were assessed for resistance to Cereal Cyst Nematode (Heterodera avenae Woll.) and 13 were found to be resistant (P = 0.05). Synthetic allohexaploid wheats produced from different Triticum turgidurn var. durum (genome AABB) lines and H. avenae resistant accessions of T tauschii maintained the level of resistance expressed in the diploids. This is the first report of resistance to H. avenae in T. tauschii, and in synthesized allohexaploid wheat. Since the resistance is in the D genome it is likely to be novel. Resistance was most common in T. tauschii ssp. eusquarrosa var. meyeri, and its derivatives which are confined to the Pahlavi region of Iran.

Diane E. Mather - One of the best experts on this subject based on the ideXlab platform.

  • Fine mapping of Rha2 in barley reveals candidate genes for resistance against Cereal Cyst Nematode
    Theoretical and Applied Genetics, 2019
    Co-Authors: Bart Van Gansbeke, Kelvin H. P. Khoo, John G. Lewis, Kenneth J. Chalmers, Diane E. Mather
    Abstract:

    Key message The Cereal Cyst Nematode resistance locus Rha2 was mapped to a 978 kbp region on the long arm of barley chromosome 2H. Three candidate genes are discussed. Abstract The Cereal Cyst Nematode (CCN) Heterodera avenae is a soil-borne obligate parasite that can cause severe damage to Cereals. This research involved fine mapping of Rha2 , a CCN resistance locus on chromosome 2H of barley. Rha2 was previously mapped relative to restriction fragment length polymorphisms (RFLPs) in two mapping populations. Anchoring of flanking RFLP clone sequences to the barley genome assembly defined an interval of 5077 kbp. Genotyping-by-sequencing of resistant and susceptible materials led to the discovery of potentially useful single nucleotide polymorphisms (SNPs). Assays were designed for these SNPs and applied to mapping populations. This narrowed the region of interest to 3966 kbp. Further fine mapping was pursued by crossing and backcrossing the resistant cultivar Sloop SA to its susceptible ancestor Sloop. Evaluation of F_2 progeny confirmed that the resistance segregates as a single dominant gene. Genotyping of 9003 BC_2F_2 progeny identified recombinants. Evaluation of recombinant BC_2F_3 progeny narrowed the region of interest to 978 kbp. Two of the SNPs within this region proved to be diagnostic of CCN resistance across a wide range of barley germplasm. Fluorescence-based and gel-based assays were developed for these SNPs for use in marker-assisted selection. Within the candidate region of the reference genome, there are nine high-confidence predicted genes. Three of these, one that encodes RAR1 (a Cysteine- and histidine-rich domain-containing protein), one that is predicted to encode an acetylglutamate kinase and one that is predicted to encode a tonoplast intrinsic protein, are discussed as candidate genes for CCN resistance.

  • Fine mapping of Rha2 in barley reveals candidate genes for resistance against Cereal Cyst Nematode.
    Theoretical and Applied Genetics, 2019
    Co-Authors: Bart Van Gansbeke, Kelvin H. P. Khoo, Kenneth J. Chalmers, John Lewis, Diane E. Mather
    Abstract:

    The Cereal Cyst Nematode resistance locus Rha2 was mapped to a 978 kbp region on the long arm of barley chromosome 2H. Three candidate genes are discussed. The Cereal Cyst Nematode (CCN) Heterodera avenae is a soil-borne obligate parasite that can cause severe damage to Cereals. This research involved fine mapping of Rha2, a CCN resistance locus on chromosome 2H of barley. Rha2 was previously mapped relative to restriction fragment length polymorphisms (RFLPs) in two mapping populations. Anchoring of flanking RFLP clone sequences to the barley genome assembly defined an interval of 5077 kbp. Genotyping-by-sequencing of resistant and susceptible materials led to the discovery of potentially useful single nucleotide polymorphisms (SNPs). Assays were designed for these SNPs and applied to mapping populations. This narrowed the region of interest to 3966 kbp. Further fine mapping was pursued by crossing and backcrossing the resistant cultivar Sloop SA to its susceptible ancestor Sloop. Evaluation of F2 progeny confirmed that the resistance segregates as a single dominant gene. Genotyping of 9003 BC2F2 progeny identified recombinants. Evaluation of recombinant BC2F3 progeny narrowed the region of interest to 978 kbp. Two of the SNPs within this region proved to be diagnostic of CCN resistance across a wide range of barley germplasm. Fluorescence-based and gel-based assays were developed for these SNPs for use in marker-assisted selection. Within the candidate region of the reference genome, there are nine high-confidence predicted genes. Three of these, one that encodes RAR1 (a Cysteine- and histidine-rich domain-containing protein), one that is predicted to encode an acetylglutamate kinase and one that is predicted to encode a tonoplast intrinsic protein, are discussed as candidate genes for CCN resistance.

John Lewis - One of the best experts on this subject based on the ideXlab platform.

  • Fine mapping of Rha2 in barley reveals candidate genes for resistance against Cereal Cyst Nematode.
    Theoretical and Applied Genetics, 2019
    Co-Authors: Bart Van Gansbeke, Kelvin H. P. Khoo, Kenneth J. Chalmers, John Lewis, Diane E. Mather
    Abstract:

    The Cereal Cyst Nematode resistance locus Rha2 was mapped to a 978 kbp region on the long arm of barley chromosome 2H. Three candidate genes are discussed. The Cereal Cyst Nematode (CCN) Heterodera avenae is a soil-borne obligate parasite that can cause severe damage to Cereals. This research involved fine mapping of Rha2, a CCN resistance locus on chromosome 2H of barley. Rha2 was previously mapped relative to restriction fragment length polymorphisms (RFLPs) in two mapping populations. Anchoring of flanking RFLP clone sequences to the barley genome assembly defined an interval of 5077 kbp. Genotyping-by-sequencing of resistant and susceptible materials led to the discovery of potentially useful single nucleotide polymorphisms (SNPs). Assays were designed for these SNPs and applied to mapping populations. This narrowed the region of interest to 3966 kbp. Further fine mapping was pursued by crossing and backcrossing the resistant cultivar Sloop SA to its susceptible ancestor Sloop. Evaluation of F2 progeny confirmed that the resistance segregates as a single dominant gene. Genotyping of 9003 BC2F2 progeny identified recombinants. Evaluation of recombinant BC2F3 progeny narrowed the region of interest to 978 kbp. Two of the SNPs within this region proved to be diagnostic of CCN resistance across a wide range of barley germplasm. Fluorescence-based and gel-based assays were developed for these SNPs for use in marker-assisted selection. Within the candidate region of the reference genome, there are nine high-confidence predicted genes. Three of these, one that encodes RAR1 (a Cysteine- and histidine-rich domain-containing protein), one that is predicted to encode an acetylglutamate kinase and one that is predicted to encode a tonoplast intrinsic protein, are discussed as candidate genes for CCN resistance.

  • genetic mapping of the cre8 locus for resistance against Cereal Cyst Nematode heterodera avenae woll in wheat
    Molecular Breeding, 2015
    Co-Authors: D V Jayatilake, John Lewis, Elise J Tucker, Julia Brueggemann, Melissa Garcia, Susanne Dreisigacker, Matthew J Hayden, K J Chalmers, D E Mather
    Abstract:

    The Cereal Cyst Nematode (CCN, Heterodera avenae Woll.) resistance locus Cre8 on the long arm of chromosome 6B (6BL) of wheat (Triticum aestivum L.) is effective in lowering the Nematode population in soil. Identification of reliable and high-throughput molecular markers linked to the Cre8 locus is important for the successful deployment of Cre8-derived resistance in wheat breeding programs. Here, we report the addition of over 600 markers to improve an existing linkage map for the Trident/Molineux wheat population. With the improved map, Cre8 was mapped as a large-effect quantitative trait locus (QTL) near the distal end of 6BL. This QTL explained up to 35 % of the phenotypic variation in CCN resistance. New marker assays were developed for DNA polymorphisms in the Cre8 region. Seven molecular markers closely linked with Cre8 (at 0.9, 2.2 or 5.9 cM from the estimated QTL position) were found to be diagnostic across a panel of wheat cultivars and are recommended for use in marker-assisted selection of the Cre8 resistance locus in wheat breeding. Prospects for the isolation of the causal gene are discussed.

  • impact of cre1 cre8 and cre3 genes on Cereal Cyst Nematode resistance in wheat
    Theoretical and Applied Genetics, 2005
    Co-Authors: E Safari, R. F. Eastwood, John Lewis, H. A. Eagles, N N Gororo, Francis C. Ogbonnaya
    Abstract:

    The Cereal Cyst Nematode (CCN; Heterodera avenae), a root disease of Cereal crops, is a major economic constraint in many wheat (Triticum aestivum)-growing areas of the world. The objective of this study was to assess the impact of the Cre1, Cre8 and Cre3 genes on CCN resistance. A population of 92 doubled-haploid (DH) lines derived from a cross between wheat cvs. Frame and Silverstar as well as 1,851 wheat breeding lines were screened for CCN resistance at the Primary Industries Research Victoria (PIRVic). A second population of 9,470 wheat breeding lines was screened at the South Australian Research and Development Institute (SARDI). Cre3 had the largest impact on reducing the number of female Cysts, followed by Cre1 and Cre8. There was no significant difference in number of Cysts between DH lines with or without the Cre8 marker, suggesting that the marker is not perfectly linked to Cre8. The estimated heritabilities were 0.32 in the DH population, 0.48 in the PIRVic data set and 0.32 in the SARDI data set, which confirm that this is a trait of low heritability. The repeatability of CCN resistance improved with an increase in the number of plants assessed per line—up to ten. However, 85–88% of the improvement was achieved with the assessments of the first five plants.

  • RFLP mapping of a new Cereal Cyst Nematode resistance locus in barley
    Plant Breeding, 1998
    Co-Authors: A. R. Barr, Kenneth J. Chalmers, John Lewis, A. Karakousis, J. M. Kretschmer, S. Manning, Reg Lance, S. P. Jeffries, Peter Langridge
    Abstract:

    Cereal Cyst Nematode (CCN) (Heterodera avenae Woll.) is an economically damaging pest of barley in many of the worlds Cereal growing areas. The development of CCN-resistant cultivars may be accelerated with the application of molecular markers. Three resistance genes against the pest have been mapped previously to chromosome 2 (Ha1, Ha2 and Ha3). In this study, a third gene present in the Australian barley variety 'Galleon' derived from the landrace 'CI3576' was located. Segregation analysis of CCN resistance data derived from doubled haploid populations of the cross 'Haruna Nijo' × 'Galleon' identified a single major locus controlling CCN resistance in the variety 'Galleon'. This locus mapped to the long arm of chromosome 5H estimated to be 6.2cM from the known function restriction fragment length polymorphism marker XYL (xylanase). While five genes for CCN resistance, including Ha2, have been mapped to group 2 chromosomes in the Triticeae, no gene other than Ha4 has been identified on group 5 chromosomes.

Kenneth J. Chalmers - One of the best experts on this subject based on the ideXlab platform.

  • Fine mapping of Rha2 in barley reveals candidate genes for resistance against Cereal Cyst Nematode
    Theoretical and Applied Genetics, 2019
    Co-Authors: Bart Van Gansbeke, Kelvin H. P. Khoo, John G. Lewis, Kenneth J. Chalmers, Diane E. Mather
    Abstract:

    Key message The Cereal Cyst Nematode resistance locus Rha2 was mapped to a 978 kbp region on the long arm of barley chromosome 2H. Three candidate genes are discussed. Abstract The Cereal Cyst Nematode (CCN) Heterodera avenae is a soil-borne obligate parasite that can cause severe damage to Cereals. This research involved fine mapping of Rha2 , a CCN resistance locus on chromosome 2H of barley. Rha2 was previously mapped relative to restriction fragment length polymorphisms (RFLPs) in two mapping populations. Anchoring of flanking RFLP clone sequences to the barley genome assembly defined an interval of 5077 kbp. Genotyping-by-sequencing of resistant and susceptible materials led to the discovery of potentially useful single nucleotide polymorphisms (SNPs). Assays were designed for these SNPs and applied to mapping populations. This narrowed the region of interest to 3966 kbp. Further fine mapping was pursued by crossing and backcrossing the resistant cultivar Sloop SA to its susceptible ancestor Sloop. Evaluation of F_2 progeny confirmed that the resistance segregates as a single dominant gene. Genotyping of 9003 BC_2F_2 progeny identified recombinants. Evaluation of recombinant BC_2F_3 progeny narrowed the region of interest to 978 kbp. Two of the SNPs within this region proved to be diagnostic of CCN resistance across a wide range of barley germplasm. Fluorescence-based and gel-based assays were developed for these SNPs for use in marker-assisted selection. Within the candidate region of the reference genome, there are nine high-confidence predicted genes. Three of these, one that encodes RAR1 (a Cysteine- and histidine-rich domain-containing protein), one that is predicted to encode an acetylglutamate kinase and one that is predicted to encode a tonoplast intrinsic protein, are discussed as candidate genes for CCN resistance.

  • Fine mapping of Rha2 in barley reveals candidate genes for resistance against Cereal Cyst Nematode.
    Theoretical and Applied Genetics, 2019
    Co-Authors: Bart Van Gansbeke, Kelvin H. P. Khoo, Kenneth J. Chalmers, John Lewis, Diane E. Mather
    Abstract:

    The Cereal Cyst Nematode resistance locus Rha2 was mapped to a 978 kbp region on the long arm of barley chromosome 2H. Three candidate genes are discussed. The Cereal Cyst Nematode (CCN) Heterodera avenae is a soil-borne obligate parasite that can cause severe damage to Cereals. This research involved fine mapping of Rha2, a CCN resistance locus on chromosome 2H of barley. Rha2 was previously mapped relative to restriction fragment length polymorphisms (RFLPs) in two mapping populations. Anchoring of flanking RFLP clone sequences to the barley genome assembly defined an interval of 5077 kbp. Genotyping-by-sequencing of resistant and susceptible materials led to the discovery of potentially useful single nucleotide polymorphisms (SNPs). Assays were designed for these SNPs and applied to mapping populations. This narrowed the region of interest to 3966 kbp. Further fine mapping was pursued by crossing and backcrossing the resistant cultivar Sloop SA to its susceptible ancestor Sloop. Evaluation of F2 progeny confirmed that the resistance segregates as a single dominant gene. Genotyping of 9003 BC2F2 progeny identified recombinants. Evaluation of recombinant BC2F3 progeny narrowed the region of interest to 978 kbp. Two of the SNPs within this region proved to be diagnostic of CCN resistance across a wide range of barley germplasm. Fluorescence-based and gel-based assays were developed for these SNPs for use in marker-assisted selection. Within the candidate region of the reference genome, there are nine high-confidence predicted genes. Three of these, one that encodes RAR1 (a Cysteine- and histidine-rich domain-containing protein), one that is predicted to encode an acetylglutamate kinase and one that is predicted to encode a tonoplast intrinsic protein, are discussed as candidate genes for CCN resistance.

  • RFLP mapping of a new Cereal Cyst Nematode resistance locus in barley
    Plant Breeding, 1998
    Co-Authors: A. R. Barr, Kenneth J. Chalmers, John Lewis, A. Karakousis, J. M. Kretschmer, S. Manning, Reg Lance, S. P. Jeffries, Peter Langridge
    Abstract:

    Cereal Cyst Nematode (CCN) (Heterodera avenae Woll.) is an economically damaging pest of barley in many of the worlds Cereal growing areas. The development of CCN-resistant cultivars may be accelerated with the application of molecular markers. Three resistance genes against the pest have been mapped previously to chromosome 2 (Ha1, Ha2 and Ha3). In this study, a third gene present in the Australian barley variety 'Galleon' derived from the landrace 'CI3576' was located. Segregation analysis of CCN resistance data derived from doubled haploid populations of the cross 'Haruna Nijo' × 'Galleon' identified a single major locus controlling CCN resistance in the variety 'Galleon'. This locus mapped to the long arm of chromosome 5H estimated to be 6.2cM from the known function restriction fragment length polymorphism marker XYL (xylanase). While five genes for CCN resistance, including Ha2, have been mapped to group 2 chromosomes in the Triticeae, no gene other than Ha4 has been identified on group 5 chromosomes.

  • RFLP mapping of the Ha 2 Cereal Cyst Nematode resistance gene in barley
    Theoretical and Applied Genetics, 1997
    Co-Authors: J. M. Kretschmer, A. R. Barr, Kenneth J. Chalmers, A. Karakousis, S. Manning, A. K. M. R. Islam, S. J. Logue, Y. W. Choe, Susan J. Barker, R. C. M. Lance
    Abstract:

    The Cereal Cyst Nematode (CCN), Heterodera avenae Woll., is an economically damaging pest of barley in many of the world’s Cereal-growing areas. The development of CCN-resistant cultivars may be accelerated through the use of molecular markers. A number of resistance genes against the pest are well known; one of them, the single dominant Ha 2 resistance gene, has been shown to be effective against the Australian pathotype and maps to chromosome 2 of barley. Segregation analysis identified two restriction fragment length polymorphism (RFLP) markers flanking the resistance gene in two doubled-haploid populations of barley. AWBMA 21 and MWG 694 mapped 4.1 and 6.1 cM respectively from the Ha 2 locus in the Chebec×Harrington cross and 4.0 and 9.2 cM respectively in the Clipper×Sahara cross. Analysis of a further seven sources of CCN resistance in the form of near-isogenic lines (NILs) indicates that all available sources of resistance to the Australian pathotype of CCN in barley represent the Ha 2 locus.

Peter Langridge - One of the best experts on this subject based on the ideXlab platform.

  • RFLP mapping of a new Cereal Cyst Nematode resistance locus in barley
    Plant Breeding, 1998
    Co-Authors: A. R. Barr, Kenneth J. Chalmers, John Lewis, A. Karakousis, J. M. Kretschmer, S. Manning, Reg Lance, S. P. Jeffries, Peter Langridge
    Abstract:

    Cereal Cyst Nematode (CCN) (Heterodera avenae Woll.) is an economically damaging pest of barley in many of the worlds Cereal growing areas. The development of CCN-resistant cultivars may be accelerated with the application of molecular markers. Three resistance genes against the pest have been mapped previously to chromosome 2 (Ha1, Ha2 and Ha3). In this study, a third gene present in the Australian barley variety 'Galleon' derived from the landrace 'CI3576' was located. Segregation analysis of CCN resistance data derived from doubled haploid populations of the cross 'Haruna Nijo' × 'Galleon' identified a single major locus controlling CCN resistance in the variety 'Galleon'. This locus mapped to the long arm of chromosome 5H estimated to be 6.2cM from the known function restriction fragment length polymorphism marker XYL (xylanase). While five genes for CCN resistance, including Ha2, have been mapped to group 2 chromosomes in the Triticeae, no gene other than Ha4 has been identified on group 5 chromosomes.

  • Development of a PCR-based allele-specific assay from an RFLP probe linked to resistance to Cereal Cyst Nematode in wheat
    Genome, 1996
    Co-Authors: Kevin J. Williams, John M. Fisher, Peter Langridge
    Abstract:

    The RFLP locus Xglk605 identified by the probe Tag605 maps to a proximal position on the long arm of wheat chromosome 2B about 7 cM away from a gene conditioning resistance to Cereal Cyst Nematode in the wheat line AUS10894. The clone Tag605 was partially sequenced and the PCR primer set AWP1 was designed. The 292-bp product, which showed no polymorphism between varieties, was cloned and sequenced. A single base difference was found in the sequence of the AWP1 products amplified and cloned from the wheat lines AUS10894 and 'Spear'. PCR primers were designed with 3′ termini that corresponded to the two alleles. A dual-PCR system was developed in which the primer sets AWP2 and AWP3 produced allele-specific amplification. The concentration of the oligonucleotide primers and the sequence of the primer–template mismatches were critical to the success of discriminatory allele amplification. Key words : Triticum aestivum, STS, Cereal Cyst Nematode, RFLP.

  • identification of rflp markers linked to the Cereal Cyst Nematode resistance gene cre in wheat
    Theoretical and Applied Genetics, 1994
    Co-Authors: K Williams, J. M. Fisher, Peter Langridge
    Abstract:

    The Cereal Cyst Nematode (CCN) (Heterodera avenae Woll.) is an economically damaging pest of wheat in many of the worlds Cereal growing areas. The development of CCN-resistant cultivars may be accelerated by the use of molecular markers. The Cre gene of the wheat line “AUS 10894” confers resistance to CCN. Using a pair of near-isogenic lines (NILs) that should differ only in a small chromosome segment containing the Cre locus, we screened 58 group-2 probes and found two (Tag605 and CDO588) that detect polymorphism between the NILs. Nulli-tetrasomic and ditelosomic lines confirmed that the restriction fragment length polymorphism (RFLP) markers identified were derived from the long arm of wheat chromosome 2. Crosses between “AUS 10894” and “Spear” and the NIL “AP” and its recurrent parent “Prins” were used to produce F2 populations that gave the expected 3∶1 segregation ratio for the resistance gene. Linkage analysis identified two RFLP markers flanking the resistance gene. Xglk605 and Xcdo588 mapped 7.3 cM (LOD=6.0) and 8.4 cM (LOD=6.7), respectively, from the Cre locus.