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Domenico Ribatti - One of the best experts on this subject based on the ideXlab platform.

  • the Chick Embryo chorioallantoic membrane
    In Vivo Models to Study Angiogenesis, 2018
    Co-Authors: Domenico Ribatti
    Abstract:

    The Chick Embryo chorioallantoic membrane (CAM) is an extraEmbryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been broadly used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of proangiogenic and antiangiogenic natural and synthetic molecules. The CAM is a favored system for the study of tumor angiogenesis and metastasis, because at this stage the Chick immunocompetence system is not fully developed and the conditions for rejection have not been established. Tumors remain avascular for 72 h, after which they are penetrated by new blood vessels and begin a phase of rapid growth. The rate of growth during this vascular phase is greater for implants on days 5–6 and decreases for later days of implantation. Other studies using the tumor cells/CAM model have focused on the invasion of the chorionic epithelium and the blood vessels by tumor cells. The cells invade the epithelium and the mesenchymal connective tissue below, where they are found in the form of a dense bed of blood vessels, which is a target for intravasation.

  • the Chick Embryo chorioallantoic membrane cam assay
    Reproductive Toxicology, 2017
    Co-Authors: Domenico Ribatti
    Abstract:

    The Chick Embryo chorioallantoic membrane (CAM) is an extraEmbryonic membrane which serves as a gas exchange surface and its function is supported by a dense capillary network. Because of its extensive vascularization and easy accessibility, the CAM has been used to study the morphofunctional aspects of the angiogenesis process in vivo and to investigate the efficacy and mechanisms of action of pro-angiogenic and anti-angiogenic natural molecules. The CAM has long been a favored system for the study of tumor angiogenesis and metastasis, because a this stage the Chick immunocompetence system is not fully developed and the conditions for rejection have not been established.

  • The Chick Embryo chorioallantoic membrane (CAM) assay
    Reproductive Toxicology, 2017
    Co-Authors: Domenico Ribatti
    Abstract:

    During avian development the mesodermal layers of the allantois and chorion fuse to form the chorioallantoic membrane (CAM). This structure rapidly expands generating a rich vascular network that provides an interface for gas and waste exchange. The CAM allows to study tissue grafts, tumor growth and metastasis, drugs delivery and toxicologic analysis, and angiogenic and anti-angiogenic molecules. The CAM is relatively simple, quick, and low-cost model that allows screening of a large number of pharmacological samples in a short time; does not require administrative procedures for obtaining ethics committee approval for animal experimentation. Moreover, being naturally immunodeficient, the Chick Embryo may receive transplantations from different tissues and species, without immune responses.

  • The Chick Embryo chorioallantoic membrane (CAM). A multifaceted experimental model
    Mechanisms of Development, 2016
    Co-Authors: Domenico Ribatti
    Abstract:

    During avian development the mesodermal layers of the allantois and chorion fuse to form the chorioallantoic membrane (CAM). This structure rapidly expands generating a rich vascular network that provides an interface for gas and waste exchange. The CAM allows to study tissue grafts, tumor growth and metastasis, wound healing, drugs delivery and toxicologic analysis, and angiogenic and anti-angiogenic molecules. The CAM is relatively simple, quick, and low-cost model that allows screening of a large number of pharmacological samples in a short time; does not require administrative procedures for obtaining ethics committee approval for animal experimentation. Moreover, being naturally immunodeficient, the Chick Embryo may receive transplantations from different tissues and species, without immune responses.

  • the Chick Embryo chorioallantoic membrane as a model for tumor biology
    Experimental Cell Research, 2014
    Co-Authors: Domenico Ribatti
    Abstract:

    Abstract Among the in vivo models, the Chick Embryo chorioallantoic membrane (CAM) has been used to implant several tumor types as well as malignant cell lines to study their growth rate, angiogenic potential and metastatic capability. This review article is focused on the major compelling literature data on the use of the CAM to investigate tumor growth and the metastatic process.

F Dammacco - One of the best experts on this subject based on the ideXlab platform.

  • the Chick Embryo chorioallantoic membrane as a model for in vivo research on angiogenesis
    The International Journal of Developmental Biology, 2004
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    The Chick Embryo chorioallantoic membrane (CAM) is an extraEmbryonic membrane that is commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Quantitative or semiquantitative methods may be used to evaluate the amount of angiogenesis and anti-angiogenesis. Thanks to the CAM system, angiogenesis could be investigated in association with normal, inflammatory and tumor tissues, and soluble factors inducing angiogenic or anti-angiogenic effects could be identified. Rabbit cornea provides an alternative in vivo system, but CAM appears to be easier to handle and less expensive. Moreover, CAM can be used with very few limitations.

  • the Chick Embryo chorioallantoic membrane as a model for in vivo research on anti angiogenesis
    Current Pharmaceutical Biotechnology, 2000
    Co-Authors: Domenico Ribatti, Angelo Vacca, Luisa Roncali, F Dammacco
    Abstract:

    Anti-angiogenesis, i.e.inhibition of blood vessel growth, is being investigated as a way to prevent the growth of tumors and other angiogenesis-dependent diseases. Pharmacological inhibition interferes with the angiogenic cascade or the immature neovasculature with synthetic or semi-synthetic substances, endogenous inhibitors or biological antagonists. The Chick Embryo chorioallantoic membrane (CAM) is an extraEmbryonic membrane commonly used in vivo to study both new vessel formation and its inhibition in response to tissues, cells, or soluble factors. Angiogenesis or anti-angiogenesis is evaluated quantitatively or semiquantitatively. The fields of application of CAM in the study of anti-angiogenesis, including our personal experience, are illustrated in this paper.

Jurgen Brieger - One of the best experts on this subject based on the ideXlab platform.

James P Quigley - One of the best experts on this subject based on the ideXlab platform.

  • Chick Embryo chorioallantoic membrane model systems to study and visualize human tumor cell metastasis
    Histochemistry and Cell Biology, 2008
    Co-Authors: Elena I Deryugina, James P Quigley
    Abstract:

    Since their introduction almost a century ago, Chick Embryo model systems involving the technique of chorioallantoic grafting have proved invaluable in the in vivo studies of tumor development and angiogenesis and tumor cell dissemination. The ability of the Chick Embryo’s chorioallantoic membrane (CAM) to efficiently support the growth of inoculated xenogenic tumor cells greatly facilitates analysis of human tumor cell metastasis. During spontaneous metastasis, the highly vascularized CAM sustains rapid tumor formation within several days following cell grafting. The dense capillary network of the CAM also serves as a repository of aggressive tumor cells that escaped from the primary tumor and intravasated into the host vasculature. This spontaneous metastasis setting provides a unique experimental model to study in vivo the intravasation step of the metastatic cascade. During experimental metastasis when tumor cells are inoculated intravenously, the CAM capillary system serves as a place for initial arrest and then, for tumor cell extravasation and colonization. The tissue composition and accessibility of the CAM for experimental interventions makes Chick Embryo CAM systems attractive models to follow the fate and visualize microscopically the behavior of grafted tumor cells in both spontaneous and experimental metastasis settings.

  • chapter 2 Chick Embryo chorioallantoic membrane models to quantify angiogenesis induced by inflammatory and tumor cells or purified effector molecules
    Methods in Enzymology, 2008
    Co-Authors: Elena I Deryugina, James P Quigley
    Abstract:

    Angiogenesis plays a critical role in many normal physiological processes as well as in tumor neovascularization associated with cancer progression. Among various animal model systems designed to study the mechanisms underlying angiogenesis, Chick Embryo models have been useful tools in analyzing the angiogenic potential of purified factors and intact cells. The chorioallantoic membrane (CAM), a specialized, highly vascularized tissue of the avian Embryo, serves as an ideal indicator of the anti‐ or pro‐angiogenic properties of test compounds. In this chapter, we describe a number basic Chick Embryo CAM models of angiogenesis. A special emphasis is on the model system employing three‐dimensional (3D) collagen grafts planted on the CAM, referred herein as onplants. This collagen onplant model allows for unambiguous quantification of angiogenesis and also for in‐depth analysis of the cellular and biochemical mechanisms by which specific cells of different origin or purified effector molecules induce or inhibit the angiogenic process.

Christoph Raphael Buhr - One of the best experts on this subject based on the ideXlab platform.