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Jonas M. Winchell - One of the best experts on this subject based on the ideXlab platform.

Irena Choroszy-król - One of the best experts on this subject based on the ideXlab platform.

  • Epidemiology of infections caused by Chlamydophila pneumoniae in patients with chronic cough
    Postępy Higieny i Medycyny Doświadczalnej, 2018
    Co-Authors: Irena Choroszy-król, Jolanta Sarowska, Agnieszka Jama-kmiecik, Dorota Teryks-wołyniec, Magdalena Frej-mądrzak
    Abstract:

    Background: Chlamydophila pneumoniae is an important etiological agent in respiratory system infections. The aim of study was to analyze the rate of Chlamydophila pneumoniae infection in adults and children and also to determine a correlation between the presence of this pathogen and symptoms of chronic cough. Material/Methods: The material for the study included swabs from the posterior pharyngeal wall taken on an empty stomach without cleaning the mouth. The diagnostic method was indirect immunofluorescence test (IIFT), which uses two types of antibodies: monoclonal mouse antibodies, which link specifically with the antigen that is present in the tested material and goat anti-mouse antibodies linked to fluorescein isothiocyanate, providing the colour reaction with C. pneumoniae antigen. Results: In our research, 593 patients, including 319 women, 175 men, aged from 18 to 87 years and a group of 99 children aged from 2 to 17 years with symptoms of chronic cough n=432 and other respiratory manifestations n=161 were studied. In the group of studied women with cough, 28.2% (64/227) of results were positive. In the group of men with cough, 22.3% (27/121) of results were positive. In the group of children with a cough, 28.6% (24/84) of the results were positive. Conclusions: In the examined group of children and adults with a chronic cough, the C. pneumoniae antigen was detected. The frequency of detection of C. pneumoniae antigen differed depending on the age group of both children and adults with symptoms of chronic cough.

  • Wybrane aspekty zakażeń Chlamydophila pneumoniae Selected aspects of Chlamydophila pneumoniae infections
    2015
    Co-Authors: Agnieszka Jama-kmiecik, Jolanta Sarowska, Magdalena Frej-mądrzak, Irena Choroszy-król
    Abstract:

    Chlamydophila pneumoniae (Chl. pneumoniae) zostala taksonomicznie wyodrebniona ze szczepu TWAR skrot od dwoch szczepow wyizolowanych od ludzi: TW-183 (material z oka dziecka na Tajwanie w 1965 r.) i AR-39 (material z wymazu z gardla studenta z ostrymi zmianami w obrebie drog oddechowych w Seattle w 1983 r.). Podstawą wyodrebnienia gatunku Chl. pneumoniae byla unikatowa struktura cialek elementarnych.

  • Selected aspects of Chlamydophila pneumoniae infections
    Index Copernicus International S.A., 2015
    Co-Authors: Agnieszka Jama-kmiecik, Jolanta Sarowska, Magdalena Frej-mądrzak, Irena Choroszy-król
    Abstract:

    Chlamydophila pneumoniae was taxonomically separated from strain TWAR – an abbreviation of the strain isolated from humans TW-183 (material from the eye of a child in Taiwan in 1965) and AR-39 (material from a student’s throat swab with acute changes within airways in Seattle in 1983). The basis of separation of the C. pneumoniae species was the unique structure of the elementary bodies.Infection caused by C. pneumoniae is often asymptomatic (60-80% of all infections). Symptomatic infections of the upper respiratory tract relate to pharyngitis, laryngitis, sinusitis and the lower respiratory tract: bronchitis and pneumonia. C. pneumoniae infection often transforms into a chronic, clinically oligo- or asymptomatic form. The chronic inflammatory process is associated by many authors with the pathogenesis of coronary artery disease, endocarditis, atherosclerosis, hypertension, vasculitis, multiple sclerosis, sarcoidosis, and asthma.C. pneumoniae has a specific tropism and exhibits cytotoxic activity towards the airway epithelium, in which it proliferates and destroys infected cells by lysis. Entry of these bacteria to the human body leads to activation of first non-specific and then specific resistance mechanisms and the development of a local inflammatory process.Diagnosis of C. pneumoniae should be confirmed only after the exclusion of typical micro-organisms causing respiratory infections. It is important to pay attention to the fact that the epidemiological data on the incidence of C. pneumoniae infections in different age groups of patients are variable depending on the type of diagnostic methods used in the research.Chlamydia are resistant to most antibiotics that are routinely used in respiratory tract infections. These bacteria are susceptible to antibiotics that disrupt the synthesis of DNA and proteins, such as macrolides, tetracyclines, and fluoroquinolones

  • Infections caused by Chlamydophila pneumoniae.
    Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2014
    Co-Authors: Irena Choroszy-król, Magdalena Frej-mądrzak, Martyna Hober, Jolanta Sarowska, Agnieszka Jama-kmiecik
    Abstract:

    High affinity to the epithelial lining of the airways makes Chlamydophila pneumoniae a common etiological agent of respiratory tract infections (RTI). It causes among others: pharyngitis, tracheitis, sinusitis, otitis media, bronchitis and bronchiolitis, and pneumonia. It is estimated that Chlamydophila pneumoniae infection is responsible for about 20% of lower respiratory tract infections. Chlamydophila pneumoniae infection may play an important role in the pathogenesis and course of bronchial asthma. The recent results indicate that Chlamydophila pneumoniae infection may be a factor responsible for 4-16% of COPD (Chronic obstructive pulmonary disease) exacerbations. A relationship of chlamydial infection with atherosclerosis raises huge interest. A connection of Chlamydophila pneumoniae infection with other non-communicable diseases such as lung cancer, arthritis, Alzheimer's disease, multiple sclerosis, sarcoidosis and erythema nodosum is also recognized, although the role of these bacteria has not been fully understood in any of the listed diseases.

  • Detection of Chlamydophila pneumoniae antigens in patients with chronic cough.
    Advances in experimental medicine and biology, 2013
    Co-Authors: Irena Choroszy-król, Jolanta Sarowska, Agnieszka Jama-kmiecik, Magdalena Frej-madrzak, Pawel Serek, Iwona Pirogowicz, Marita Nittner-marszalska
    Abstract:

    The aim of this study was to analyze the rate of Chlamydophila pneumoniae infection in adults with symptoms of chronic cough. The study was conducted in 83 hospitalized patients aged 18–67 suffering of chronic cough. The control group consisted of 20 healthy age-matched subjects without any respiratory symptoms. Bacteriological tests on the presence of Chlamydophila pneumoniae antigen were performed in throat swabs by indirect immunofluorescence technique using monoclonal antibodies labeled with fluorescein isothiocyanate. The rate of Chlamydophila infected patients was examined in relation to age and gender. The Chlamydophila pneumoniae antigen was detected in 15 (18 %) out of the 83 patients; about equally in both genders. Furthermore, we found that the patients aged 28–37 constituted the age group that most frequently tested positive for Chlamydophila pneumoniae. Unraveling the presence of Chlamydia infection in chronic cough patients enables to introduce a timely implementation of effective therapy and thus can prevent distant complications.

Maureen H. Diaz - One of the best experts on this subject based on the ideXlab platform.

José Gutiérrez - One of the best experts on this subject based on the ideXlab platform.

  • Emerging strategies in the diagnosis, prevention and treatment of Chlamydophila pneumoniae infections
    Expert Opinion on Therapeutic Patents, 2008
    Co-Authors: Enrique Villegas, Ana Camacho, Jose Antonio Carrillo, Antonio Sorlózano, José Rojas, José Gutiérrez
    Abstract:

    Background: Chlamydophila pneumoniae infections are a common cause of acute respiratory diseases, including upper respiratory tract infections and pneumonia. Over the past few years, C. pneumoniae infections have been strongly related to atherosclerotic cardiovascular diseases. Objective: The aim of this review is to offer an update and overview of recent advances in the diagnosis, prevention and treatment of these infections. Methods: Diagnostic systems have improved but further progress is required to allow a reliable diagnosis to be made. This is especially true for atherosclerotic diseases, for which standard criteria need to be established. Results/conclusion: Polymerase chain reaction and serological methods need to be standardized and made better to improve the diagnosis of C. pneumoniae infections. It seems to be crucial to obtain new and more selective antigens associated with persistent infections to explain the participation of C. pneumoniae in coronary artery disease.

  • ELISA test to detect Chlamydophila pneumoniae IgG.
    Journal of basic microbiology, 2002
    Co-Authors: José Gutiérrez, J. Mendoza, F. Fernández, José Patricio Linares-palomino, María José Soto, María Del Carmen Maroto
    Abstract:

    A new ELISA test (Chlamydophila pneumoniae IgG, Vircell, Spain) to detect Chlamydophila pneumoniae IgG was evaluated. The micro-immunofluorescence (MIF) test was used as reference method. Chlamydia trachomatis and Chlamydophila psittaci elementary bodies were also assayed. Two hundred and sixteen sera were included in the study: 66 from patients with peripheral arterial occlusive disease (Panel 1), 68 from adults with pneumonia (Panel 2), 44 from healthy adults (Panel 3) and 38 from patients with a sexuality transmitted disease by C. trachomatis (Panel 4). In Panel 1, 51 sera (77%) had antibody titres between 32 and 128; 4 out of 15 sera with IgG titres < 32 were positive by ELISA test and 2 sera with 32 IgG titres were uncertain by ELISA; the remaining 60 sera were correctly classified, giving a 91% concordance between the techniques. In Panel 2, 55 sera (81%) had IgG titres between 32 and 512; 2 out of 13 sera with IgG titres < 32 were positive by ELISA and 2 sera with 32 titres were uncertain by ELISA; the remaining 64 sera were correctly classified, giving a 97% concordance. In Panel 3, 22 sera (50%) had IgG titres between 32 and 64; only 1 out of 22 sera with IgG titres < 32 was positive by ELISA, giving a 97% concordance between the techniques. In Panel 4, there were 24 (63%) negative, 10 (26%) uncertain and 4 (10%) positive results by ELISA, giving an 86% concordance. The C. pneumoniae ELISA test demonstrated 100% sensitivity and 85% specificity. The IgG ELISA test demonstrated a good concordance with the MIF test without the drawbacks associated with the latter assay. We conclude that the ELISA test could be an alternative to the MIF test.

Michael E. Ward - One of the best experts on this subject based on the ideXlab platform.

  • Characterization of human humoral responses to the major outer membrane protein and OMP2 of Chlamydophila pneumoniae
    FEMS microbiology letters, 2003
    Co-Authors: Adam F. Cunningham, Michael E. Ward
    Abstract:

    Chlamydophila pneumoniae infection is associated with a range of diseases including pneumonia, asthma and heart disease. Although an obligate intracellular pathogen, high levels of antigen-specific antibody are induced and serology is frequently used to diagnose these infections. Proteins targeted by the humoral response include the major outer membrane protein (MOMP) and outer membrane protein 2 (OMP2). Using human anti-chlamydial sera we have defined the B cell epitopes recognized on MOMP and OMP2. Peptides from MOMP, unlike OMP2, were not strongly recognized. Two of these epitopes when linked to an inert carrier reacted strongly with high-titer anti-C. pneumoniae sera.