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Koichi Makimura - One of the best experts on this subject based on the ideXlab platform.
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immune response in human Chromoblastomycosis and eumycetoma focusing on human interleukin 17a interferon gamma tumour necrosis factor alpha interleukin 1 beta and human beta defensin 2
Mycoses, 2016Co-Authors: Charussri Leeyaphan, Carren Sy Hau, Shintaro Takeoka, Yayoi Tada, Sumanas Bunyaratavej, Penvadee Pattanaprichakul, Panitta Sitthinamsuwan, Angkana Chaiprasert, Yuko Sasajima, Koichi MakimuraAbstract:Summary Knowledge regarding host immune response to Chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin-embedded tissue samples from patients diagnosed with Chromoblastomycosis or eumycetoma from histological findings and tissue culture. DNA extraction and polymerase chain reaction (PCR) from tissues were done to evaluate human interleukin-17A (IL-17A), interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and human beta-defensin-2 (HBD-2) expressions. Human beta-actin primer was used for confirming DNA detection, and DNA extracted from psoriasis lesional skin samples was used as positive controls. The twelve paraffin-embedded sections used in this study consisted of five Chromoblastomycosis and seven eumycetoma tissues. All PCR reactions showed beta-actin band at 51 bp in all clinical specimens, confirming adequate DNA levels in each reaction. As positive control, the psoriasis skin samples revealed bands for IL-17A at 174 bp, IFN-γ at 273 bp, TNF-α at 360 bp, IL-1β at 276 bp and HBD-2 at 255 bp. For the Chromoblastomycosis and eumycetoma tissues, PCR analyses showed IL-17A band at 174 bp in two eumycetoma tissues and HBD-2 band at 255 bp in a Chromoblastomycosis tissue. This study demonstrated IL-17A expression in human eumycetoma and HBD-2 expression in human Chromoblastomycosis for the first time. However, their role in immune response remains to be elucidated.
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Immune response in human Chromoblastomycosis and eumycetoma – focusing on human interleukin‐17A, interferon‐gamma, tumour necrosis factor‐alpha, interleukin‐1 beta and human beta‐defensin‐2
Mycoses, 2016Co-Authors: Charussri Leeyaphan, Carren Sy Hau, Shintaro Takeoka, Yayoi Tada, Sumanas Bunyaratavej, Penvadee Pattanaprichakul, Panitta Sitthinamsuwan, Angkana Chaiprasert, Yuko Sasajima, Koichi MakimuraAbstract:Summary Knowledge regarding host immune response to Chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin-embedded tissue samples from patients diagnosed with Chromoblastomycosis or eumycetoma from histological findings and tissue culture. DNA extraction and polymerase chain reaction (PCR) from tissues were done to evaluate human interleukin-17A (IL-17A), interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and human beta-defensin-2 (HBD-2) expressions. Human beta-actin primer was used for confirming DNA detection, and DNA extracted from psoriasis lesional skin samples was used as positive controls. The twelve paraffin-embedded sections used in this study consisted of five Chromoblastomycosis and seven eumycetoma tissues. All PCR reactions showed beta-actin band at 51 bp in all clinical specimens, confirming adequate DNA levels in each reaction. As positive control, the psoriasis skin samples revealed bands for IL-17A at 174 bp, IFN-γ at 273 bp, TNF-α at 360 bp, IL-1β at 276 bp and HBD-2 at 255 bp. For the Chromoblastomycosis and eumycetoma tissues, PCR analyses showed IL-17A band at 174 bp in two eumycetoma tissues and HBD-2 band at 255 bp in a Chromoblastomycosis tissue. This study demonstrated IL-17A expression in human eumycetoma and HBD-2 expression in human Chromoblastomycosis for the first time. However, their role in immune response remains to be elucidated.
Maria Lucia Scroferneker - One of the best experts on this subject based on the ideXlab platform.
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in vitro susceptibility of Chromoblastomycosis agents to five antifungal drugs and to the combination of terbinafine and amphotericin b
Mycoses, 2014Co-Authors: Tatiane Caroline Daboit, Cibele Massotti Magagnin, Daiane Heidrich, Laura Czekster Antochevis, Suelen Vigolo, Lucia Collares Meirelles, Karine De Oliveira Alves, Maria Lucia ScrofernekerAbstract:Summary Chromoblastomycosis is a chronic mycosis that affects the skin and subcutaneous tissues caused by several genera of dematiaceous fungi. There is not a treatment of choice. Thus, tools that help guide clinical practice are fundamental. In this sense, antifungal activity tests in vitro could be useful. However, trials with Chromoblastomycosis agents are scarce. The aim of this study was to evaluate both the in vitro susceptibility of 60 Chromoblastomycosis agents to five antifungals and the combination of amphotericin B (AMB) and terbinafine (TRB). TRB, itraconazole (ITZ) and ketoconazole (KTZ) were, in this order, the drugs which showed better activity against the Chromoblastomycosis agents. The less active drugs were voriconazole (VRZ) and AMB. The more differentiated group was Exophiala spinifera. Cladophialophora carrionii and Fonsecaea spp. are significantly more susceptible to KTZ than Phialophora verrucosa, whereas C. carrionii is significantly more sensitive to VRZ than P. verrucosa and E. spinifera. Assays in this direction allow the knowledge of the susceptibility of the causative agents which may help the management of patients with this disease. This study includes the largest number of these agents and of genera found in the literature.
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Chromoblastomycosis a review of 100 cases in the state of rio grande do sul brazil
Journal of The American Academy of Dermatology, 2001Co-Authors: Renan Minotto, Cesar Duilio Varejao Bernardi, Luis Felipe Mallmann, Maria Isabel Albano Edelweiss, Maria Lucia ScrofernekerAbstract:Abstract Background: If not diagnosed earlier, Chromoblastomycosis has a chronic evolutional course that may cause several problems, such as difficulty in managing therapy because of the recrudescent character of the disease, potential association with the growth of epidermoid carcinoma in affected regions, and poor quality of life and work incapacity to the patient. Although infrequent, new cases are reported in the state of Rio Grande do Sul every year, ratifying the necessity for further studies on this disease. Objective: The purpose of this study was to review clinical features and response to therapy in patients with Chromoblastomycosis and present data on the demography and history of this disease in the state of Rio Grande do Sul, Brazil. Methods: We reviewed case records of 100 patients with skin lesions caused by Chromoblastomycosis, who were treated between 1963 and 1998. The cases were confirmed by the histopathologic and mycologic analyses made by the Dermatology Service of the Universidade Federal do Rio Grande do Sul at the Santa Casa de Misericordia Hospital. Results: There was a predominance of male patients (4:1) and of white farmers whose ages ranged from 50 to 59 years, with lesions on their lower limbs. Most of them were from the northern regions of the state. The average time between the appearance of the disease and medical diagnosis was 14 years. The verrucous type proved to be the most frequently reported lesion (53%). Thorn wounds were associated with the disease in 16% of the cases. Lesions uncommon to some parts of the body were also reported. In two of the cases, cutaneous lesions caused by paracoccidioidomycosis and Chromoblastomycosis were found in the same patient. Epidermoid carcinoma was found in the same parts of the body affected by Chromoblastomycosis. Eumycotic mycetoma and Chromoblastomycosis were associated. Fonsecaea pedrosoi was found in 96% of the cases, and Phialophora verrucosa in 4% of the cases. Conclusion: In our study, we observed a predominance of cases in the regions of Missoes and Alto Uruguay, followed by the upper and lower northeastern slopes and the lowlands. Severe cases of Chromoblastomycosis with intense skin involvement (eg, lesions with carcinoma) were observed. Statistical analysis showed recrudescence of the disease in 43% of cases despite the treatment used. (J Am Acad Dermatol 2001;44:585-92.)
Charussri Leeyaphan - One of the best experts on this subject based on the ideXlab platform.
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immune response in human Chromoblastomycosis and eumycetoma focusing on human interleukin 17a interferon gamma tumour necrosis factor alpha interleukin 1 beta and human beta defensin 2
Mycoses, 2016Co-Authors: Charussri Leeyaphan, Carren Sy Hau, Shintaro Takeoka, Yayoi Tada, Sumanas Bunyaratavej, Penvadee Pattanaprichakul, Panitta Sitthinamsuwan, Angkana Chaiprasert, Yuko Sasajima, Koichi MakimuraAbstract:Summary Knowledge regarding host immune response to Chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin-embedded tissue samples from patients diagnosed with Chromoblastomycosis or eumycetoma from histological findings and tissue culture. DNA extraction and polymerase chain reaction (PCR) from tissues were done to evaluate human interleukin-17A (IL-17A), interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and human beta-defensin-2 (HBD-2) expressions. Human beta-actin primer was used for confirming DNA detection, and DNA extracted from psoriasis lesional skin samples was used as positive controls. The twelve paraffin-embedded sections used in this study consisted of five Chromoblastomycosis and seven eumycetoma tissues. All PCR reactions showed beta-actin band at 51 bp in all clinical specimens, confirming adequate DNA levels in each reaction. As positive control, the psoriasis skin samples revealed bands for IL-17A at 174 bp, IFN-γ at 273 bp, TNF-α at 360 bp, IL-1β at 276 bp and HBD-2 at 255 bp. For the Chromoblastomycosis and eumycetoma tissues, PCR analyses showed IL-17A band at 174 bp in two eumycetoma tissues and HBD-2 band at 255 bp in a Chromoblastomycosis tissue. This study demonstrated IL-17A expression in human eumycetoma and HBD-2 expression in human Chromoblastomycosis for the first time. However, their role in immune response remains to be elucidated.
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Immune response in human Chromoblastomycosis and eumycetoma – focusing on human interleukin‐17A, interferon‐gamma, tumour necrosis factor‐alpha, interleukin‐1 beta and human beta‐defensin‐2
Mycoses, 2016Co-Authors: Charussri Leeyaphan, Carren Sy Hau, Shintaro Takeoka, Yayoi Tada, Sumanas Bunyaratavej, Penvadee Pattanaprichakul, Panitta Sitthinamsuwan, Angkana Chaiprasert, Yuko Sasajima, Koichi MakimuraAbstract:Summary Knowledge regarding host immune response to Chromoblastomycosis and eumycetoma is limited, particularly concerning cytokines and antimicrobial peptides production. This was a retrospective study of 12 paraffin-embedded tissue samples from patients diagnosed with Chromoblastomycosis or eumycetoma from histological findings and tissue culture. DNA extraction and polymerase chain reaction (PCR) from tissues were done to evaluate human interleukin-17A (IL-17A), interferon-gamma (IFN-γ), tumour necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and human beta-defensin-2 (HBD-2) expressions. Human beta-actin primer was used for confirming DNA detection, and DNA extracted from psoriasis lesional skin samples was used as positive controls. The twelve paraffin-embedded sections used in this study consisted of five Chromoblastomycosis and seven eumycetoma tissues. All PCR reactions showed beta-actin band at 51 bp in all clinical specimens, confirming adequate DNA levels in each reaction. As positive control, the psoriasis skin samples revealed bands for IL-17A at 174 bp, IFN-γ at 273 bp, TNF-α at 360 bp, IL-1β at 276 bp and HBD-2 at 255 bp. For the Chromoblastomycosis and eumycetoma tissues, PCR analyses showed IL-17A band at 174 bp in two eumycetoma tissues and HBD-2 band at 255 bp in a Chromoblastomycosis tissue. This study demonstrated IL-17A expression in human eumycetoma and HBD-2 expression in human Chromoblastomycosis for the first time. However, their role in immune response remains to be elucidated.
Junmin Zhang - One of the best experts on this subject based on the ideXlab platform.
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photodynamic therapy combined with terbinafine against Chromoblastomycosis and the effect of pdt on fonsecaea monophora in vitro
Mycopathologia, 2015Co-Authors: Xiaowen Huang, Michael R Hamblin, Eleftherios Mylonakis, Junmin ZhangAbstract:Chromoblastomycosis, a chronic fungal infection of skin and subcutaneous tissue caused by dematiaceous fungi, is associated with low cure and high relapse rates. Among all factors affecting clinical outcome, etiological agents have an important position. In southern China, Fonsecaea pedrosoi and Fonsecaea monophora are main causative agents causing Chromoblastomycosis. We treated one case of Chromoblastomycosis by photodynamic therapy (PDT) of 5-aminolevulinic acid (ALA) irradiation combined with terbinafine 250 mg a day. The lesions were improved after two sessions of ALA-PDT treatment, each including nine times, at an interval of 1 week, combined with terbinafine 250 mg/day oral, and clinical improvement could be observed. In the following study, based on the clinical treatment, the effect of PDT and antifungal drugs on this isolate was detected in vitro. It showed sensitivity to terbinafine, itraconazole or voriconazole, and PDT inhibited the growth. Both the clinic and experiments in vitro confirm the good outcome of ALA-PDT applied in the inhibition of F. monophora. It demonstrated that combination of antifungal drugs with ALA-PDT arises as a promising alternative method for the treatment of these refractory cases of Chromoblastomycosis.
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a refractory case of Chromoblastomycosis due to fonsecaea monophora with improvement by photodynamic therapy
Medical Mycology, 2012Co-Authors: Yabo Yang, Junmin Zhang, Yuheng LiangAbstract:Chromoblastomycosis is one of the most frequently encountered mycoses in tropical and temperate regions caused by the implantation of the infectious structures and one which is associated with low cure and high relapse rates. The etiologic agents play a critical role affecting clinical outcome and in southern China, Fonsecaea pedrosoi and F. monophora are the main causative agents of Chromoblastomycosis. We treated, for two years, a 55-year-old male patient with Chromoblastomycosis caused by F. monophora with itraconazole and terbinafine, two antifungals recommend in earlier papers in the literature but without any positive response. As a result we introduced the photodynamic therapy (PDT) employing 5-aminolevulinic acid (ALA) irradiation. The lesions were improved after two periods of ALA-PDT treatment, each consisting of exposures at weekly intervals for 5 weeks but new lesions developed with the cessation of ALA-PDT treatment. Thereafter, positive clinical improvement was obtained when voriconazole at ...
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Successful treatment for Chromoblastomycosis caused by Fonsecaea monophora: a report of three cases in Guangdong, China.
Mycoses, 2008Co-Authors: Junmin Zhang, Zhi Xie, Ting Xie, Hui Zhang, De Hoog SybrenAbstract:Fonsecaea pedrosoi is the most prevalent aetiological agent of Chromoblastomycosis. Fonsecaea monophora is a new species segregated from Fonsecaea pedrosoi. Herein, we report on three cases of Chromoblastomycosis caused by F. monophora that were successfully treated with terbinafine and/or itraconazole. Clinical characteristics and mycological parameters are described. Two of the three patients underwent combination therapy with itraconazole and terbinafine during early stages of treatment and were completely healed in a relatively short course of treatment.
Yasuhito Hamaguchi - One of the best experts on this subject based on the ideXlab platform.
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Chromoblastomycosis caused by Phialophora verrucosa on the hand
European journal of dermatology : EJD, 2015Co-Authors: Akiko Takeuchi, Kazufumi Anzawa, Takashi Mochizuki, Kazuhiko Takehara, Yasuhito HamaguchiAbstract:The fungi that form black colonies on culture medium due to melanin pigment in the cell wall are collectively referred to as dematiaceous fungi. Dematiaceous fungal infections with a verrucous or plaque appearance and histopathologically sclerotic or muriform cells are classified as Chromoblastomycosis. Phialophora verrucosa (P. verrucosa) is one of the Chromoblastomycosis-causing fungi [1]. Itis common in the environment but infrequently causes an infection due to its low pathogenicity [2]. Here, [...]