The Experts below are selected from a list of 17190 Experts worldwide ranked by ideXlab platform
Florencia Bullrich - One of the best experts on this subject based on the ideXlab platform.
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Chromosomal Mapping of cell death proteases CPP32, MCH2, and MCH3
Genomics, 1996Co-Authors: Florencia Bullrich, Teresa Fernandes-alnemri, Gerald Litwack, Emad S. Alnemri, Carlo M. CroceAbstract:Apoptosis may involve a specialized proteolytic cascade catalyzed by interleukin-1β-converting enzyme-like proteases. We have recently identified three new members of this family (CPP32, MCH2, MCH3) and shown that they play an important role in promoting cell death. Here we report the Chromosomal Mapping of CPP32 to 4q34, MCH2 to 4q25, and MCH3 to 10q25.
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Chromosomal Mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer.
Cancer research, 1995Co-Authors: Florencia Bullrich, Teresa Druck, Timothy K. Maclachlan, Nianli Sang, Maria Luisa Veronese, Steven L. Allen, Nicholas Chiorazzi, Andrew Koff, Kay Heubner, Carlo M. CroceAbstract:Abstract Orderly progression through the cell cycle requires sequential activation and inactivation of cyclin-dependent kinases (cdks). This is achieved in part through the association of cdks with positive regulators called cyclins and inactivation of cyclin-cdk complexes by a rapidly growing number of cyclin-cdk inhibitors. Recently, the role of cell cycle control proteins both as primary effectors and as mediators of tumorigenesis has become a subject of increased interest. Here we report the Chromosomal Mapping of two cdks, cdk3 and cdk6, two putative cdks, PISSLRE and PITALRE, and one cyclin-dependent kinase inhibitor, p27, to Chromosomal regions which may be altered in human tumors and examine their possible involvement in some of these malignancies. In particular, two of the kinases, cdk3 and PISSLRE and PITALRE, the cdc2-related kinases recently cloned by us, map to regions previously shown to exhibit loss of heterozygosity in breast and other tumors.
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Chromosomal Mapping of the genes GPRK5 and GPRK6 encoding G protein-coupled receptor kinases GRK5 and GRK6
Cytogenetics and cell genetics, 1995Co-Authors: Florencia Bullrich, Teresa Druck, Priya Kunapuli, Jorge Gomez, K. W. Gripp, Brigitte Schlegelberger, Jerzy Lasota, M. Aronson, Linda A. Cannizzaro, Kay HuebnerAbstract:G protein-coupled receptor kinases (GRKs) play an important role in phosphorylating and regulating the activity of a variety of G protein-coupled receptors. Chromosomal Mapping of the human genes for
Shigeko Kijimoto-ochiai - One of the best experts on this subject based on the ideXlab platform.
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Cloning, Chromosomal Mapping, and Characteristic 5′-UTR Sequence of Murine Cytosolic Sialidase
Biochemical and biophysical research communications, 2001Co-Authors: Kiyoshi Kotani, Asato Kuroiwa, Tamao Saito, Yoichi Matsuda, Toshiaki Koda, Shigeko Kijimoto-ochiaiAbstract:Abstract We have totally sequenced a cytosolic sialidase [EC 3.2.1.18] by RT-PCR from the murine thymus (murine thymic sialidase, MTS) which has a 1844-base length (encoding 385 amino acids including two sialidase motifs) and is the longest cytosolic sialidase ever reported. MTS has high and relatively low homologies with those of mammalian cytosolic sialidases from the mouse brain (99%), rat (91%), and human skeletal muscle (75%), and those of the mouse lysosomal (47%) and membrane-bound (51%) sialidases, respectively. Chromosomal Mapping, being the first report of mouse cytosolic sialidase gene, showed that the MTS gene is localized to the distal part of mouse chromosome 1D and to rat chromosome 9q36. RT-PCR with the site-specific primers revealed that the coding region was expressed in all organs tested, but expressions including the 5′-UTR were barely detectable except for in the upper-thymic fraction. Also, soluble sialidase activity in the thymus was the highest of these organs. There were mRNA instability signals and AT-rich regions in 143 bp of MTS 5′-end.
Debra J Gilbert - One of the best experts on this subject based on the ideXlab platform.
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Organization and Chromosomal Mapping of mouse Gh/tissue transglutaminase gene (Tgm2).
Archives of biochemistry and biophysics, 1999Co-Authors: Nisha Nanda, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Siiri E. Iismaa, Robert M. Graham, Pramod SutraveAbstract:The mouse Gh/tissue transglutaminase gene (Tgm2), coding a dual-function protein that both binds guanosine triphosphate (GTP) and catalyzes the posttranslational modification of proteins by transamidation of glutamine residues, has been cloned. Sequence analysis of Tgm2 and comparison with the TGase sequences of other species allowed correction of several apparent sequencing artifacts in the Tgm2 cDNA. Tgm2 spans approximately 34 kb and has 13 exons and 12 introns. Although the structure of Tgm2 shows similarity to that of other transglutaminase genes, with introns ranging from 921 bp to >5 kb, several introns differ considerably in size from those of the human Gh gene, TGM2. Tgm2 maps to the distal region of mouse chromosome 2, a region syntenic to human chromosome 20q containing TGM2. Tgm2 is in the vicinity of two uncloned mouse mutations, diminutive (dm) and blind-sterile (bs). Genomic DNA from dm mice was unavailable; however, Southern blot analysis of bs DNA showed no gross rearrangements of Tgm2.
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Genetic structure and Chromosomal Mapping of MyD88.
Genomics, 1997Co-Authors: Gary Hardiman, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Dawn K. Garcia, Susan L. Naylor, Robert A. Kastelein, J. Fernando BazanAbstract:Abstract The myeloid differentiation (MyD) markerMyD88was initially characterized as a primary response gene, upregulated in mouse M1 myeloleukemic cells in response to differentiation induced by interleukin-6. Subsequent analysis revealed thatMyD88possesses a unique modular structure, which consists of an N-terminal “death domain,” similar to the intracellular segments of TNF receptor 1 and Fas, and a C-terminal region related to the cytoplasmic domains of theDrosophilamorphogen Toll and vertebrate interleukin-1 receptors. In this report we describe the cloning and gene structure of mouseMyD88.The complete coding sequence of mouseMyD88spans five exons, with the first exon encoding the complete death domain. Zooblot analysis revealed thatMyD88is an evolutionarily conserved gene.MyD88was localized to the distal region of mouse chromosome 9 by interspecific backcross Mapping. The human homolog (hMyD88) was mapped to chromosome 3p22–p21.3 by PCR analysis of a human chromosome 3 somatic cell hybrid Mapping panel. Northern blot analysis revealed widespread expression ofMyD88in many adult mouse tissues, and RT-PCR studies detectedMyD88mRNA in T and B cell lines and differentiating embryonic stem cells. The broad expression pattern demonstrates that mouseMyD88expression is not restricted to cells of myeloid lineage as was originally believed.
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in situ localization and Chromosomal Mapping of the ag1 dmp1 gene
Journal of Histochemistry and Cytochemistry, 1994Co-Authors: Anne George, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Arthur VeisAbstract:Dentinogenesis is being used as a model for understanding the biomineralization process. The odontoblasts synthesize a structural matrix comprised of Type I collagen fibrils which define the basic architecture of the tissue. The odontoblasts also synthesize and deliver a number of dentin-specific acidic macromolecules into the extracellular compartment. These acidic macromolecules may be involved in regulating the ordered deposition of hydroxyapatite crystals within the matrix. AG1 is the first tooth-specific acidic macromolecule to have been cloned and sequenced. To identify which cells of the rat incisor pulp/odontoblast complex were responsible for synthesis of AG1, in situ hybridization was used. Digoxigenin labeled sense and anti-sense AG1 riboprobes were prepared. The AG1 mRNA was found to be expressed in the mature secretory odontoblasts. Neither pulp cells nor pre-odontoblasts showed any staining with the anti-sense probes. Chromosomal localization studies placed the AG1 gene on mouse chromosome 5...
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molecular cloning of the murine st2 gene characterization and Chromosomal Mapping
Biochimica et Biophysica Acta, 1991Co-Authors: Shinichi Tominaga, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Tsunao TetsukaAbstract:The genomic locus of the murine ST2 gene was isolated based on homology with a murine ST2 complementary DNA sequence and its complete nucleotide sequence was determined. The locus is composed of eight exons and seven introns and is approx. 9 kilobase pairs in size. Two Sp1 binding sites are present in the 5' flanking region. The murine ST2 gene, which was expressed only in the growth-stimulated BALB/c-3T3 cells, was mapped to mouse chromosome one, very tightly linked to the interleukin 1 receptor-type 1 locus.
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genomic organization and Chromosomal Mapping of the mouse p cadherin gene
Nucleic Acids Research, 1991Co-Authors: Masayuki Hatta, Debra J Gilbert, Seiji Miyatani, Nancy A Jenkins, Neal G Copeland, Masatoshi TakeichiAbstract:Abstract Cadherins are a family of Ca(2+)-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45 kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23 kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the correspoding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the Chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes.
Nancy A Jenkins - One of the best experts on this subject based on the ideXlab platform.
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Organization and Chromosomal Mapping of mouse Gh/tissue transglutaminase gene (Tgm2).
Archives of biochemistry and biophysics, 1999Co-Authors: Nisha Nanda, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Siiri E. Iismaa, Robert M. Graham, Pramod SutraveAbstract:The mouse Gh/tissue transglutaminase gene (Tgm2), coding a dual-function protein that both binds guanosine triphosphate (GTP) and catalyzes the posttranslational modification of proteins by transamidation of glutamine residues, has been cloned. Sequence analysis of Tgm2 and comparison with the TGase sequences of other species allowed correction of several apparent sequencing artifacts in the Tgm2 cDNA. Tgm2 spans approximately 34 kb and has 13 exons and 12 introns. Although the structure of Tgm2 shows similarity to that of other transglutaminase genes, with introns ranging from 921 bp to >5 kb, several introns differ considerably in size from those of the human Gh gene, TGM2. Tgm2 maps to the distal region of mouse chromosome 2, a region syntenic to human chromosome 20q containing TGM2. Tgm2 is in the vicinity of two uncloned mouse mutations, diminutive (dm) and blind-sterile (bs). Genomic DNA from dm mice was unavailable; however, Southern blot analysis of bs DNA showed no gross rearrangements of Tgm2.
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Genetic structure and Chromosomal Mapping of MyD88.
Genomics, 1997Co-Authors: Gary Hardiman, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Dawn K. Garcia, Susan L. Naylor, Robert A. Kastelein, J. Fernando BazanAbstract:Abstract The myeloid differentiation (MyD) markerMyD88was initially characterized as a primary response gene, upregulated in mouse M1 myeloleukemic cells in response to differentiation induced by interleukin-6. Subsequent analysis revealed thatMyD88possesses a unique modular structure, which consists of an N-terminal “death domain,” similar to the intracellular segments of TNF receptor 1 and Fas, and a C-terminal region related to the cytoplasmic domains of theDrosophilamorphogen Toll and vertebrate interleukin-1 receptors. In this report we describe the cloning and gene structure of mouseMyD88.The complete coding sequence of mouseMyD88spans five exons, with the first exon encoding the complete death domain. Zooblot analysis revealed thatMyD88is an evolutionarily conserved gene.MyD88was localized to the distal region of mouse chromosome 9 by interspecific backcross Mapping. The human homolog (hMyD88) was mapped to chromosome 3p22–p21.3 by PCR analysis of a human chromosome 3 somatic cell hybrid Mapping panel. Northern blot analysis revealed widespread expression ofMyD88in many adult mouse tissues, and RT-PCR studies detectedMyD88mRNA in T and B cell lines and differentiating embryonic stem cells. The broad expression pattern demonstrates that mouseMyD88expression is not restricted to cells of myeloid lineage as was originally believed.
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in situ localization and Chromosomal Mapping of the ag1 dmp1 gene
Journal of Histochemistry and Cytochemistry, 1994Co-Authors: Anne George, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Arthur VeisAbstract:Dentinogenesis is being used as a model for understanding the biomineralization process. The odontoblasts synthesize a structural matrix comprised of Type I collagen fibrils which define the basic architecture of the tissue. The odontoblasts also synthesize and deliver a number of dentin-specific acidic macromolecules into the extracellular compartment. These acidic macromolecules may be involved in regulating the ordered deposition of hydroxyapatite crystals within the matrix. AG1 is the first tooth-specific acidic macromolecule to have been cloned and sequenced. To identify which cells of the rat incisor pulp/odontoblast complex were responsible for synthesis of AG1, in situ hybridization was used. Digoxigenin labeled sense and anti-sense AG1 riboprobes were prepared. The AG1 mRNA was found to be expressed in the mature secretory odontoblasts. Neither pulp cells nor pre-odontoblasts showed any staining with the anti-sense probes. Chromosomal localization studies placed the AG1 gene on mouse chromosome 5...
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molecular cloning of the murine st2 gene characterization and Chromosomal Mapping
Biochimica et Biophysica Acta, 1991Co-Authors: Shinichi Tominaga, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Tsunao TetsukaAbstract:The genomic locus of the murine ST2 gene was isolated based on homology with a murine ST2 complementary DNA sequence and its complete nucleotide sequence was determined. The locus is composed of eight exons and seven introns and is approx. 9 kilobase pairs in size. Two Sp1 binding sites are present in the 5' flanking region. The murine ST2 gene, which was expressed only in the growth-stimulated BALB/c-3T3 cells, was mapped to mouse chromosome one, very tightly linked to the interleukin 1 receptor-type 1 locus.
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genomic organization and Chromosomal Mapping of the mouse p cadherin gene
Nucleic Acids Research, 1991Co-Authors: Masayuki Hatta, Debra J Gilbert, Seiji Miyatani, Nancy A Jenkins, Neal G Copeland, Masatoshi TakeichiAbstract:Abstract Cadherins are a family of Ca(2+)-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45 kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23 kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the correspoding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the Chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes.
Neal G Copeland - One of the best experts on this subject based on the ideXlab platform.
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Organization and Chromosomal Mapping of mouse Gh/tissue transglutaminase gene (Tgm2).
Archives of biochemistry and biophysics, 1999Co-Authors: Nisha Nanda, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Siiri E. Iismaa, Robert M. Graham, Pramod SutraveAbstract:The mouse Gh/tissue transglutaminase gene (Tgm2), coding a dual-function protein that both binds guanosine triphosphate (GTP) and catalyzes the posttranslational modification of proteins by transamidation of glutamine residues, has been cloned. Sequence analysis of Tgm2 and comparison with the TGase sequences of other species allowed correction of several apparent sequencing artifacts in the Tgm2 cDNA. Tgm2 spans approximately 34 kb and has 13 exons and 12 introns. Although the structure of Tgm2 shows similarity to that of other transglutaminase genes, with introns ranging from 921 bp to >5 kb, several introns differ considerably in size from those of the human Gh gene, TGM2. Tgm2 maps to the distal region of mouse chromosome 2, a region syntenic to human chromosome 20q containing TGM2. Tgm2 is in the vicinity of two uncloned mouse mutations, diminutive (dm) and blind-sterile (bs). Genomic DNA from dm mice was unavailable; however, Southern blot analysis of bs DNA showed no gross rearrangements of Tgm2.
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Genetic structure and Chromosomal Mapping of MyD88.
Genomics, 1997Co-Authors: Gary Hardiman, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Dawn K. Garcia, Susan L. Naylor, Robert A. Kastelein, J. Fernando BazanAbstract:Abstract The myeloid differentiation (MyD) markerMyD88was initially characterized as a primary response gene, upregulated in mouse M1 myeloleukemic cells in response to differentiation induced by interleukin-6. Subsequent analysis revealed thatMyD88possesses a unique modular structure, which consists of an N-terminal “death domain,” similar to the intracellular segments of TNF receptor 1 and Fas, and a C-terminal region related to the cytoplasmic domains of theDrosophilamorphogen Toll and vertebrate interleukin-1 receptors. In this report we describe the cloning and gene structure of mouseMyD88.The complete coding sequence of mouseMyD88spans five exons, with the first exon encoding the complete death domain. Zooblot analysis revealed thatMyD88is an evolutionarily conserved gene.MyD88was localized to the distal region of mouse chromosome 9 by interspecific backcross Mapping. The human homolog (hMyD88) was mapped to chromosome 3p22–p21.3 by PCR analysis of a human chromosome 3 somatic cell hybrid Mapping panel. Northern blot analysis revealed widespread expression ofMyD88in many adult mouse tissues, and RT-PCR studies detectedMyD88mRNA in T and B cell lines and differentiating embryonic stem cells. The broad expression pattern demonstrates that mouseMyD88expression is not restricted to cells of myeloid lineage as was originally believed.
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in situ localization and Chromosomal Mapping of the ag1 dmp1 gene
Journal of Histochemistry and Cytochemistry, 1994Co-Authors: Anne George, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Arthur VeisAbstract:Dentinogenesis is being used as a model for understanding the biomineralization process. The odontoblasts synthesize a structural matrix comprised of Type I collagen fibrils which define the basic architecture of the tissue. The odontoblasts also synthesize and deliver a number of dentin-specific acidic macromolecules into the extracellular compartment. These acidic macromolecules may be involved in regulating the ordered deposition of hydroxyapatite crystals within the matrix. AG1 is the first tooth-specific acidic macromolecule to have been cloned and sequenced. To identify which cells of the rat incisor pulp/odontoblast complex were responsible for synthesis of AG1, in situ hybridization was used. Digoxigenin labeled sense and anti-sense AG1 riboprobes were prepared. The AG1 mRNA was found to be expressed in the mature secretory odontoblasts. Neither pulp cells nor pre-odontoblasts showed any staining with the anti-sense probes. Chromosomal localization studies placed the AG1 gene on mouse chromosome 5...
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molecular cloning of the murine st2 gene characterization and Chromosomal Mapping
Biochimica et Biophysica Acta, 1991Co-Authors: Shinichi Tominaga, Debra J Gilbert, Nancy A Jenkins, Neal G Copeland, Tsunao TetsukaAbstract:The genomic locus of the murine ST2 gene was isolated based on homology with a murine ST2 complementary DNA sequence and its complete nucleotide sequence was determined. The locus is composed of eight exons and seven introns and is approx. 9 kilobase pairs in size. Two Sp1 binding sites are present in the 5' flanking region. The murine ST2 gene, which was expressed only in the growth-stimulated BALB/c-3T3 cells, was mapped to mouse chromosome one, very tightly linked to the interleukin 1 receptor-type 1 locus.
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genomic organization and Chromosomal Mapping of the mouse p cadherin gene
Nucleic Acids Research, 1991Co-Authors: Masayuki Hatta, Debra J Gilbert, Seiji Miyatani, Nancy A Jenkins, Neal G Copeland, Masatoshi TakeichiAbstract:Abstract Cadherins are a family of Ca(2+)-dependent cell adhesion molecules, that includes P-cadherin, E-cadherin, N-cadherin and L-CAM. In this study, the genomic organization of the mouse P-cadherin gene was determined by analyzing overlapping DNA clones obtained from a mouse genomic library. The results showed that this gene spans over 45 kb and consists of 15 exons. A marked feature of this gene is that the first intron is 23 kbp long accounting for half its length. Comparisons of this structure with that of L-CAM, a chicken cadherin, revealed that the exon-intron boundaries are conserved between the two genes except that the P-cadherin first exon includes the correspoding first and second exons of the L-CAM gene. This gene was also similar to the other in that the second intron, which corresponds to the P-cadherin first intron, is exceptionally longer than other introns. These results suggest that the exon-intron pattern conserved in these genes is of significance for generation of domain structure of cadherin molecules or for their transcriptional regulation. We also determined the Chromosomal localization of the P-cadherin gene by interspecific backcross analysis, and found that this gene is located in the central region of mouse chromosome 8 and linked with the E-cadherin locus. This is the first evidence for the linkage of different cadherin genes.