The Experts below are selected from a list of 214743 Experts worldwide ranked by ideXlab platform
Lihui Li - One of the best experts on this subject based on the ideXlab platform.
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physical mapping of agropyron cristatum Chromosome 6p using deletion lines in common wheat background
Theoretical and Applied Genetics, 2016Co-Authors: Liqiang Song, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Key message Genetically stable deletion lines of Agropyron cristatum Chromosome 6p in common wheat background were generated, which allowed for physical mapping of 255 6p-specific STS markers and leaf rust resistance gene(s).
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the effects of Chromosome 6p on fertile tiller number of wheat as revealed in wheat agropyron cristatum Chromosome 5a 6p translocation lines
Theoretical and Applied Genetics, 2015Co-Authors: Xueling Ye, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Guoyue Chen, Lihui LiAbstract:Key message This study explored the genetic constitutions of several wheat-A. cristatum translocation lines and determined the effects of A. cristatum6p Chromosome segments on fertile tiller number in wheat.
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genetic rearrangements of six wheat agropyron cristatum 6p addition lines revealed by molecular markers
PLOS ONE, 2014Co-Authors: Junji Su, Liqiang Song, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat–A. cristatum alien disomic addition lines to locate the desired genes on the P genome Chromosomes. Comparative analysis of the homoeologous relationships between the P genome Chromosome and wheat genome Chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat–A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, Chromosome 6p had undergone obvious rearrangements in different addition lines compared with the wheat Chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat Chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6pI, 6pII, 6pIII, and 6pIV. The different types of Chromosome 6p possessed different desirable genes. For example, the 6pI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional Chromosome engineering techniques toward molecular Chromosome engineering.
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efficient induction of wheat agropyron cristatum 6p translocation lines and gish detection
PLOS ONE, 2013Co-Authors: Lili Jiang, Xinming Yang, Lihui LiAbstract:The narrow genetic background restricts wheat yield and quality improvement. The wild relatives of wheat are the huge gene pools for wheat improvement and can broaden its genetic basis. Production of wheat-alien translocation lines can transfer alien genes to wheat. So it is important to develop an efficient method to induce wheat-alien Chromosome translocation. Agropyroncristatum (P genome) carries many potential genes beneficial to disease resistance, stress tolerance and high yield. Chromosome 6p possesses the desirable genes exhibiting good agronomic traits, such as high grain number per spike, powdery mildew resistance and stress tolerance. In this study, the wheat-A. cristatum disomic addition was used as bridge material to produce wheat-A. cristatum translocation lines induced by 60Co-γirradiation. The results of genomic in situ hybridization showed that 216 plants contained alien Chromosome translocation among 571 self-pollinated progenies. The frequency of translocation was 37.83%, much higher than previous reports. Moreover, various alien translocation types were identified. The analysis of M2 showed that 62.5% of intergeneric translocation lines grew normally without losing the translocated Chromosomes. The paper reported a high efficient technical method for inducing alien translocation between wheat and Agropyroncristatum. Additionally, these translocation lines will be valuable for not only basic research on genetic balance, interaction and expression of different Chromosome segments of wheat and alien species, but also wheat breeding programs to utilize superior agronomic traits and good compensation effect from alien Chromosomes.
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production and identification of wheat agropyron cristatum 6p translocation lines
Planta, 2010Co-Authors: Yang Luan, Jinpeng Zhang, Xinming Yang, Xiaoguang Wang, Chunye Li, Yandong Wang, Lihui LiAbstract:The narrow genetic background of wheat is the primary factor that has restricted the improvement of crop yield in recent years. The kernel number per spike is the most important factor of the many potential characteristics that determine wheat yield. Agropyron cristatum (L.) Gaertn., a wild relative of wheat, has the characteristics of superior numbers of florets and kernels per spike, which are controlled by Chromosome 6p. In this study, the wheat-A. cristatum disomic addition and substitution lines were used as bridge materials to produce wheat-A. cristatum 6p translocation lines induced by gametocidal Chromosomes and irradiation. The results of genomic in situ hybridization showed that the frequency of translocation induced by gametocidal Chromosomes was 5.08%, which was higher than the frequency of irradiated hybrids (2.78%) and irradiated pollen (2.12%). The fluorescence in situ hybridization results of the translocation lines showed that A. cristatum Chromosome 6p could be translocated to wheat ABD genome, and the recombination frequency was A genome > B genome > D genome. The alien A. cristatum Chromosome 6p was translocated to wheat homoeologous groups 1, 2, 3, 5 and 6. We obtained abundant translocation lines that possessed whole-arm, terminal, segmental and intercalary translocations. Three 6pS-specific and four 6pL-specific markers will be useful to rapidly identify and trace the translocated fragments. The different wheat-A. cristatum 6p translocation lines obtained in this study can provide basic materials for analyzing the alien genes carried by Chromosome 6p. The translocation line WAT33-1-3 and introgression lines WAI37-2 and WAI41-1, which had significant characteristics of multikernel (high numbers of kernels per spike), could be utilized as novel germplasms for high-yield wheat breeding.
Xinming Yang - One of the best experts on this subject based on the ideXlab platform.
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physical mapping of agropyron cristatum Chromosome 6p using deletion lines in common wheat background
Theoretical and Applied Genetics, 2016Co-Authors: Liqiang Song, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Key message Genetically stable deletion lines of Agropyron cristatum Chromosome 6p in common wheat background were generated, which allowed for physical mapping of 255 6p-specific STS markers and leaf rust resistance gene(s).
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the effects of Chromosome 6p on fertile tiller number of wheat as revealed in wheat agropyron cristatum Chromosome 5a 6p translocation lines
Theoretical and Applied Genetics, 2015Co-Authors: Xueling Ye, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Guoyue Chen, Lihui LiAbstract:Key message This study explored the genetic constitutions of several wheat-A. cristatum translocation lines and determined the effects of A. cristatum6p Chromosome segments on fertile tiller number in wheat.
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genetic rearrangements of six wheat agropyron cristatum 6p addition lines revealed by molecular markers
PLOS ONE, 2014Co-Authors: Junji Su, Liqiang Song, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat–A. cristatum alien disomic addition lines to locate the desired genes on the P genome Chromosomes. Comparative analysis of the homoeologous relationships between the P genome Chromosome and wheat genome Chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat–A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, Chromosome 6p had undergone obvious rearrangements in different addition lines compared with the wheat Chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat Chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6pI, 6pII, 6pIII, and 6pIV. The different types of Chromosome 6p possessed different desirable genes. For example, the 6pI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional Chromosome engineering techniques toward molecular Chromosome engineering.
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efficient induction of wheat agropyron cristatum 6p translocation lines and gish detection
PLOS ONE, 2013Co-Authors: Lili Jiang, Xinming Yang, Lihui LiAbstract:The narrow genetic background restricts wheat yield and quality improvement. The wild relatives of wheat are the huge gene pools for wheat improvement and can broaden its genetic basis. Production of wheat-alien translocation lines can transfer alien genes to wheat. So it is important to develop an efficient method to induce wheat-alien Chromosome translocation. Agropyroncristatum (P genome) carries many potential genes beneficial to disease resistance, stress tolerance and high yield. Chromosome 6p possesses the desirable genes exhibiting good agronomic traits, such as high grain number per spike, powdery mildew resistance and stress tolerance. In this study, the wheat-A. cristatum disomic addition was used as bridge material to produce wheat-A. cristatum translocation lines induced by 60Co-γirradiation. The results of genomic in situ hybridization showed that 216 plants contained alien Chromosome translocation among 571 self-pollinated progenies. The frequency of translocation was 37.83%, much higher than previous reports. Moreover, various alien translocation types were identified. The analysis of M2 showed that 62.5% of intergeneric translocation lines grew normally without losing the translocated Chromosomes. The paper reported a high efficient technical method for inducing alien translocation between wheat and Agropyroncristatum. Additionally, these translocation lines will be valuable for not only basic research on genetic balance, interaction and expression of different Chromosome segments of wheat and alien species, but also wheat breeding programs to utilize superior agronomic traits and good compensation effect from alien Chromosomes.
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production and identification of wheat agropyron cristatum 6p translocation lines
Planta, 2010Co-Authors: Yang Luan, Jinpeng Zhang, Xinming Yang, Xiaoguang Wang, Chunye Li, Yandong Wang, Lihui LiAbstract:The narrow genetic background of wheat is the primary factor that has restricted the improvement of crop yield in recent years. The kernel number per spike is the most important factor of the many potential characteristics that determine wheat yield. Agropyron cristatum (L.) Gaertn., a wild relative of wheat, has the characteristics of superior numbers of florets and kernels per spike, which are controlled by Chromosome 6p. In this study, the wheat-A. cristatum disomic addition and substitution lines were used as bridge materials to produce wheat-A. cristatum 6p translocation lines induced by gametocidal Chromosomes and irradiation. The results of genomic in situ hybridization showed that the frequency of translocation induced by gametocidal Chromosomes was 5.08%, which was higher than the frequency of irradiated hybrids (2.78%) and irradiated pollen (2.12%). The fluorescence in situ hybridization results of the translocation lines showed that A. cristatum Chromosome 6p could be translocated to wheat ABD genome, and the recombination frequency was A genome > B genome > D genome. The alien A. cristatum Chromosome 6p was translocated to wheat homoeologous groups 1, 2, 3, 5 and 6. We obtained abundant translocation lines that possessed whole-arm, terminal, segmental and intercalary translocations. Three 6pS-specific and four 6pL-specific markers will be useful to rapidly identify and trace the translocated fragments. The different wheat-A. cristatum 6p translocation lines obtained in this study can provide basic materials for analyzing the alien genes carried by Chromosome 6p. The translocation line WAT33-1-3 and introgression lines WAI37-2 and WAI41-1, which had significant characteristics of multikernel (high numbers of kernels per spike), could be utilized as novel germplasms for high-yield wheat breeding.
Xiuquan Li - One of the best experts on this subject based on the ideXlab platform.
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physical mapping of agropyron cristatum Chromosome 6p using deletion lines in common wheat background
Theoretical and Applied Genetics, 2016Co-Authors: Liqiang Song, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Key message Genetically stable deletion lines of Agropyron cristatum Chromosome 6p in common wheat background were generated, which allowed for physical mapping of 255 6p-specific STS markers and leaf rust resistance gene(s).
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the effects of Chromosome 6p on fertile tiller number of wheat as revealed in wheat agropyron cristatum Chromosome 5a 6p translocation lines
Theoretical and Applied Genetics, 2015Co-Authors: Xueling Ye, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Guoyue Chen, Lihui LiAbstract:Key message This study explored the genetic constitutions of several wheat-A. cristatum translocation lines and determined the effects of A. cristatum6p Chromosome segments on fertile tiller number in wheat.
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genetic rearrangements of six wheat agropyron cristatum 6p addition lines revealed by molecular markers
PLOS ONE, 2014Co-Authors: Junji Su, Liqiang Song, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat–A. cristatum alien disomic addition lines to locate the desired genes on the P genome Chromosomes. Comparative analysis of the homoeologous relationships between the P genome Chromosome and wheat genome Chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat–A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, Chromosome 6p had undergone obvious rearrangements in different addition lines compared with the wheat Chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat Chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6pI, 6pII, 6pIII, and 6pIV. The different types of Chromosome 6p possessed different desirable genes. For example, the 6pI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional Chromosome engineering techniques toward molecular Chromosome engineering.
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the introgression of Chromosome 6p specifying for increased numbers of florets and kernels from agropyron cristatum into wheat
Theoretical and Applied Genetics, 2006Co-Authors: Jun Wu, Lihui Li, Xinmin Yang, Hui Wang, Hongjie Li, Xiuquan LiAbstract:A wheat (Triticum aestivum L.) line 4844 with superior numbers of florets and grains per spike was derived from the cross between Fukohokomugi wheat and Agropyron cristatum (L.) Gaertn. In order to determine the genetic control of floret and kernel number per spike in this line, Chromosome addition and substitution lines that were derived from line 4844 were characterized by means of in situ hybridization, microsatellite (SSR), and gliadin analyses. Genomic in situ hybridization analysis with biotinylated P genomic DNA of A. cristatum as a probe demonstrated that the increased number of florets and grains in a spike was associated with the introgression of an A. cristatum Chromosome. Fluorescence in situ hybridization, using a repetitive sequence, pAs1, derived from Aegilops squarrosa L., indicated the replacement of Chromosome 6D of wheat in the wheat-A. cristatum Chromosome substitution lines. This was confirmed by microsatellite analyses with wheat SSR markers specific for Chromosome 6D, suggesting that the A. cristatum Chromosome was homoeologous to group 6 and was therefore designated as 6p. This conclvsion was further confirmed by amplification using EST-SSR markers and gliadin analysis. The increased number of florets and kernels within a spike of the wheat-A. cristatum hybrids thus was controlled by gene(s) located on A. cristatum Chromosome 6p.
Jinpeng Zhang - One of the best experts on this subject based on the ideXlab platform.
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physical mapping of agropyron cristatum Chromosome 6p using deletion lines in common wheat background
Theoretical and Applied Genetics, 2016Co-Authors: Liqiang Song, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Key message Genetically stable deletion lines of Agropyron cristatum Chromosome 6p in common wheat background were generated, which allowed for physical mapping of 255 6p-specific STS markers and leaf rust resistance gene(s).
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the effects of Chromosome 6p on fertile tiller number of wheat as revealed in wheat agropyron cristatum Chromosome 5a 6p translocation lines
Theoretical and Applied Genetics, 2015Co-Authors: Xueling Ye, Yuqing Lu, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Guoyue Chen, Lihui LiAbstract:Key message This study explored the genetic constitutions of several wheat-A. cristatum translocation lines and determined the effects of A. cristatum6p Chromosome segments on fertile tiller number in wheat.
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genetic rearrangements of six wheat agropyron cristatum 6p addition lines revealed by molecular markers
PLOS ONE, 2014Co-Authors: Junji Su, Liqiang Song, Xiuquan Li, Jinpeng Zhang, Xinming Yang, Lihui LiAbstract:Agropyron cristatum (L.) Gaertn. (2n = 4x = 28, PPPP) not only is cultivated as pasture fodder but also could provide many desirable genes for wheat improvement. It is critical to obtain common wheat–A. cristatum alien disomic addition lines to locate the desired genes on the P genome Chromosomes. Comparative analysis of the homoeologous relationships between the P genome Chromosome and wheat genome Chromosomes is a key step in transferring different desirable genes into common wheat and producing the desired alien translocation line while compensating for the loss of wheat chromatin. In this study, six common wheat–A. cristatum disomic addition lines were produced and analyzed by phenotypic examination, genomic in situ hybridization (GISH), SSR markers from the ABD genomes and STS markers from the P genome. Comparative maps, six in total, were generated and demonstrated that all six addition lines belonged to homoeologous group 6. However, Chromosome 6p had undergone obvious rearrangements in different addition lines compared with the wheat Chromosome, indicating that to obtain a genetic compensating alien translocation line, one should recombine alien chromosomal regions with homoeologous wheat Chromosomes. Indeed, these addition lines were classified into four types based on the comparative mapping: 6pI, 6pII, 6pIII, and 6pIV. The different types of Chromosome 6p possessed different desirable genes. For example, the 6pI type, containing three addition lines, carried genes conferring high numbers of kernels per spike and resistance to powdery mildew, important traits for wheat improvement. These results may prove valuable for promoting the development of conventional Chromosome engineering techniques toward molecular Chromosome engineering.
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production and identification of wheat agropyron cristatum 6p translocation lines
Planta, 2010Co-Authors: Yang Luan, Jinpeng Zhang, Xinming Yang, Xiaoguang Wang, Chunye Li, Yandong Wang, Lihui LiAbstract:The narrow genetic background of wheat is the primary factor that has restricted the improvement of crop yield in recent years. The kernel number per spike is the most important factor of the many potential characteristics that determine wheat yield. Agropyron cristatum (L.) Gaertn., a wild relative of wheat, has the characteristics of superior numbers of florets and kernels per spike, which are controlled by Chromosome 6p. In this study, the wheat-A. cristatum disomic addition and substitution lines were used as bridge materials to produce wheat-A. cristatum 6p translocation lines induced by gametocidal Chromosomes and irradiation. The results of genomic in situ hybridization showed that the frequency of translocation induced by gametocidal Chromosomes was 5.08%, which was higher than the frequency of irradiated hybrids (2.78%) and irradiated pollen (2.12%). The fluorescence in situ hybridization results of the translocation lines showed that A. cristatum Chromosome 6p could be translocated to wheat ABD genome, and the recombination frequency was A genome > B genome > D genome. The alien A. cristatum Chromosome 6p was translocated to wheat homoeologous groups 1, 2, 3, 5 and 6. We obtained abundant translocation lines that possessed whole-arm, terminal, segmental and intercalary translocations. Three 6pS-specific and four 6pL-specific markers will be useful to rapidly identify and trace the translocated fragments. The different wheat-A. cristatum 6p translocation lines obtained in this study can provide basic materials for analyzing the alien genes carried by Chromosome 6p. The translocation line WAT33-1-3 and introgression lines WAI37-2 and WAI41-1, which had significant characteristics of multikernel (high numbers of kernels per spike), could be utilized as novel germplasms for high-yield wheat breeding.
Nienke Essed - One of the best experts on this subject based on the ideXlab platform.
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A new syndrome with noncompaction cardiomyopathy, bradycardia, pulmonary stenosis, atrial septal defect and heterotaxy with suggestive linkage to Chromosome 6p
Human Genetics, 2008Co-Authors: Marja W. Wessels, Bianca M. Graaf, Titia E. Cohen-overbeek, Silja E. Spitaels, Lotte E. Groot-de Laat, Folkert J. Ten Cate, Ingrid F. M. Frohn-mulder, Ronald Krijger, Margot M. Bartelings, Nienke EssedAbstract:We report a three-generation family with nine patients affected by a combination of cardiac abnormalities and left isomerism which, to our knowledge, has not been described before. The cardiac anomalies include non-compaction of the ventricular myocardium, bradycardia, pulmonary valve stenosis, and secundum atrial septal defect. The laterality sequence anomalies include left bronchial isomerism, azygous continuation of the inferior vena cava, polysplenia and intestinal malrotation, all compatible with left isomerism. This new syndrome is inherited in an autosomal dominant pattern. A genome-wide linkage analysis suggested linkage to Chromosome 6p24.3–21.2 with a maximum LOD score of 2.7 at marker D6S276 . The linkage interval is located between markers D6S470 (telomeric side) and D6S1610 (centromeric side), and overlaps with the linkage interval in another family with heterotaxy reported previously. Taken together, the genomic region could be reduced to 9.4 cM (12 Mb) containing several functional candidate genes for this complex heterotaxy phenotype.