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Ramona Crina Gales - One of the best experts on this subject based on the ideXlab platform.

  • STRUCTURAL CHARACTERISTICS OF Chrysanthemum Morifolium RAMAT (ROMICA CULTIVAR) REGENERATED IN VITRO
    Planta Medica, 2009
    Co-Authors: Smaranda Vantu, Ramona Crina Gales
    Abstract:

    The micropropagation of Chrysanthemum Morifolium Ramat (Romica cultivar), belonging to the collection of “Anastasie Ftu” Botanical Garden from Iasi (Romania) was achieved through tissue culture technique and involved callus induction followed by shoot multiplication, rooting and establishment of plantlets in soil. The purpose of this study was to determine the range of variation in certain structural characters of the vegetative organs of in vitro regenerated plants at Chrysanthemum Morifolium Ramat (Romica cultivar). The material subjected to the comparative anatomical analyses was represented by vegetative organs of the parent plant (PP) and regenerated plant (RP), on mature stage . The density of glandular and non-glandular hair (mm -2 ) on both leaf surfaces was statistical analysed using “t” test at 0,05

  • Structural characteristics of Chrysanthemum Morifolium Ramat (Romica cultivar)regenerated in vitro
    2009
    Co-Authors: Smaranda Vantu, Ramona Crina Gales
    Abstract:

    The micropropagation of Chrysanthemum Morifolium Ramat (Romica cultivar), belonging to the collection of “Anastasie F�tu” Botanical Garden from Iasi (Romania) was achieved through tissue culture technique and involved callus induction followed by shoot multiplication, rooting and establishment of plantlets in soil. The purpose of this study was to determine the range of variation in certain structural characters of the vegetative organs of in vitro regenerated plants at Chrysanthemum Morifolium Ramat (Romica cultivar). The material subjected to the comparative anatomical analyses was represented by vegetative organs of the parent plant (PP) and regenerated plant (RP), on mature stage . The density of glandular and non-glandular hair (mm-2) on both leaf surfaces was statistical analysed using t test at 0,05 confidence level. Despite the great opportunity of genetic variation in callus cultures, the regenerated plants differ not in their structural appearance from the normal plants.

Smaranda Vantu - One of the best experts on this subject based on the ideXlab platform.

  • STRUCTURAL CHARACTERISTICS OF Chrysanthemum Morifolium RAMAT (ROMICA CULTIVAR) REGENERATED IN VITRO
    Planta Medica, 2009
    Co-Authors: Smaranda Vantu, Ramona Crina Gales
    Abstract:

    The micropropagation of Chrysanthemum Morifolium Ramat (Romica cultivar), belonging to the collection of “Anastasie Ftu” Botanical Garden from Iasi (Romania) was achieved through tissue culture technique and involved callus induction followed by shoot multiplication, rooting and establishment of plantlets in soil. The purpose of this study was to determine the range of variation in certain structural characters of the vegetative organs of in vitro regenerated plants at Chrysanthemum Morifolium Ramat (Romica cultivar). The material subjected to the comparative anatomical analyses was represented by vegetative organs of the parent plant (PP) and regenerated plant (RP), on mature stage . The density of glandular and non-glandular hair (mm -2 ) on both leaf surfaces was statistical analysed using “t” test at 0,05

  • Structural characteristics of Chrysanthemum Morifolium Ramat (Romica cultivar)regenerated in vitro
    2009
    Co-Authors: Smaranda Vantu, Ramona Crina Gales
    Abstract:

    The micropropagation of Chrysanthemum Morifolium Ramat (Romica cultivar), belonging to the collection of “Anastasie F�tu” Botanical Garden from Iasi (Romania) was achieved through tissue culture technique and involved callus induction followed by shoot multiplication, rooting and establishment of plantlets in soil. The purpose of this study was to determine the range of variation in certain structural characters of the vegetative organs of in vitro regenerated plants at Chrysanthemum Morifolium Ramat (Romica cultivar). The material subjected to the comparative anatomical analyses was represented by vegetative organs of the parent plant (PP) and regenerated plant (RP), on mature stage . The density of glandular and non-glandular hair (mm-2) on both leaf surfaces was statistical analysed using t test at 0,05 confidence level. Despite the great opportunity of genetic variation in callus cultures, the regenerated plants differ not in their structural appearance from the normal plants.

Liu De-hui - One of the best experts on this subject based on the ideXlab platform.

  • Effects of Applying Selenium on Contents of Total Flavonoid, Chlorogenic Acid and Selenium in Flower of Chrysanthemum Morifolium
    2010
    Co-Authors: Liu De-hui
    Abstract:

    In order to investigate the effects of different application does of selenium (Se) on the contents of total flavonoid, chlorogenic acid and selenium in the flower of Chrysanthemum Morifolium, a sand culture experiment was conducted. The results showed that, it promoted the plant growth and increased the contents of total flavonoid, chlorogenic acid, when Se applying doses did not exceed 2.0 mg/kg. The dry weights of flower, stem-leaf, root and the contents of total flavonoid, chlorogenic acid increased with the increase of Se applying doses, the optimal effect was obtained when Se at 2.0 mg/kg. The dry weight of flower, root and the contents of chlorogenic acid of the plants in the treatment of Se at 2.0 mg/kg were significantly higher than Se applications at 0, 0.25, 0.5 mg/kg, and the former’ contents of total flavonoid was higher than Se application at 0, 0.25, 0.5, 1.0, 4.0 mg/kg. Excessive Se applying dose, such as at 4.0 mg/kg inhibited the growth of the plants, the dry weight of flower, stem-leaf, root and the contents of total flavonoid, chlorogenic acid were lower than Se at 2.0 mg/kg. The contents of Se in flower, stem-leaf and root of Chrysanthemum Morifolium increased with the increase of Se application doses. The relationship between Se contents in stem-leaf and root of Chrysanthemum Morifolium and Se application rate is liner, while the relationship between Se content in flower(y) and Se application rate(x) fits an equation (y =-0.817 5x2 + 6.004 5x + 0.136 3). It showed that the suitable Se applying doses could significantly increase the contents of total flavonoid, chlorogenic acid and Se content in the flower. The above results provide the theoretical basis for the development of Se-enriched Chrysanthemum Morifolium and its products.

  • Effects of fertilization on yields and the content of total flavonoid, chlorogenic acid of Chrysanthemum Morifolium
    Journal of Nanjing Agricultural University, 2007
    Co-Authors: Yan Guang-xuan, Liu De-hui
    Abstract:

    To study effects of fertilization on yield and active components of Chrysanthemum Morifolium,pig manure,chicken manure,rape cake,inorganic fertilizer were applied in the pot experiment as the doses as in planting base of Chinese medical herbs in Jiangsu Province.The results showed that all the fertilizers applied could improve the botanical characteristics,the biomass of Chrysanthemum Morifolium was increased significantly.Pig manure,chicken manure and rape cake could improve effectively total flavonoid,chlorogenic acid of Chrysanthemum Morifolium,among them the rape cake was dramatically increased total flavonoid and chlorogenic acid of Chrysanthemum Morifolium,and the pig manure was in the second place.

Yan Guang-xuan - One of the best experts on this subject based on the ideXlab platform.

Lu Juan - One of the best experts on this subject based on the ideXlab platform.

  • Research on Ultrasonic-assisted Extraction of Total Flavonoids from Chrysanthemum Morifolium
    Journal of Anhui Agricultural Sciences, 2012
    Co-Authors: Lu Juan
    Abstract:

    [Objective] The research aimed to optimize the influencing factors of the ultrasonic-assisted extraction of flavonoids from Chrysanthemum Morifolium and increase the extraction rate of flavonoids.[Method] The effects of four factors(the ratio of Chrysanthemum Morifolium to liquid of ethanol,time of ultrasonic disposing,extracting time and extracting temperature) on the extraction rate of flavonoids were studied by single-factor experiment and multi-factor orthogonal test.[Result] The best extracting conditions of flavonoids by ultrasonic-assisted ethanol extraction method were as follows: material-liquid ratio of 1∶30 g/ml,ultrasonic disposing time of 50 min,extracting at 80 ℃ for 80 min.Under these conditions the extraction rate of flavonoids was up to 11.32%.[Conclusion] Optimization of the extraction conditions increase the extraction rate of flavonoids,which laid a foundation for the development and utilization of Chrysanthemum Morifolium.