The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Xuebo Liu - One of the best experts on this subject based on the ideXlab platform.
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preservation of Cichoric Acid antioxidant properties loaded in heat treated lactoferrin nanoparticles
Molecules, 2018Co-Authors: Caicai Zhao, Liping Wei, Fuguo Liu, Min Zhang, Xuebo Liu, Yutang WangAbstract:In the current research, a new Cichoric Acid (CA) encapsulation system was investigated. The optimal condition for the formation of lactoferrin-Cichoric Acid nanoparticles (LF-CA NPs) was determined by controlling the solution pH, the thermal treatment conditions, and the concentration of CA. Fluorescence indicated that the electrostatic force and the hydrophobic force were the main forces in the formation of LF-CA NPs. LF-CA NPs prepared under different conditions were spherical in shape with smaller particle sizes and good zeta potential demonstrating good colloidal stability. Especially, the prepared particle size of the LF-CA NPs at pH 7 and 95 °C was about 67.20 ± 1.86 nm. The circular dichroism (CD) and the Fourier transform infrared spectroscopy (FTIR) results showed that the combination of LF (lactoferrin) and CA affected the secondary structure of the LF. The differential scanning calorimetry (DSC) results indicated that the addition of CA increased the thermal stability of LF. In vitro antioxidant experiments confirmed the antioxidant capacity of LF-CA NPs was better than CA. CA was successfully encapsulated into LF NPs with high encapsulated efficiency (97.87–99.87%) by high performance liquid chromatography (HPLC). These results showed that LF could be used as the wall material of CA with excellent nature.
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Cichoric Acid prevents free fatty Acid induced lipid metabolism disorders via regulating bmal1 in hepg2 cells
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Rui Guo, Yutang Wang, Zhigang Liu, Beita Zhao, Yijie Wang, Yuchen Yan, Wentong Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during the process of CA-attenuated lipid accumulation in hepatocytes. In the current study, CA treatments improved cell morphology changes and hepatic lipid levels, which were triggered by free fatty Acids (2:1, oleate: palmitate) in a dose-dependent way. Besides, CA (200 μM) regulated the circadian rhythm expressions of clock genes and the relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked the p-Akt/Akt pathway to 80.1% ± 1.5% and the p-GSK3β/GSK3β pathway to 64.7% ± 2.8% (p < 0.05). Furthermore, silencing Bmal1 elevated the expressions of FAS and ACC to 122.4% ± 5.6% and 114.9% ± 1.7% in protein levels (p < 0.05) and to 166.5% ± 18.5% and 131.4% ± 5.5% in mRNA levels (p < 0.05). Therefore, our results demonstrated that CA has a Bmal1 resistance to lipid a...
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Cichoric Acid Prevents Free-Fatty-Acid-Induced Lipid Metabolism Disorders via Regulating Bmal1 in HepG2 Cells
2018Co-Authors: Rui Guo, Yutang Wang, Zhigang Liu, Beita Zhao, Yijie Wang, Yuchen Yan, Wentong Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during the process of CA-attenuated lipid accumulation in hepatocytes. In the current study, CA treatments improved cell morphology changes and hepatic lipid levels, which were triggered by free fatty Acids (2:1, oleate: palmitate) in a dose-dependent way. Besides, CA (200 μM) regulated the circadian rhythm expressions of clock genes and the relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked the p-Akt/Akt pathway to 80.1% ± 1.5% and the p-GSK3β/GSK3β pathway to 64.7% ± 2.8% (p < 0.05). Furthermore, silencing Bmal1 elevated the expressions of FAS and ACC to 122.4% ± 5.6% and 114.9% ± 1.7% in protein levels (p < 0.05) and to 166.5% ± 18.5% and 131.4% ± 5.5% in mRNA levels (p < 0.05). Therefore, our results demonstrated that CA has a Bmal1 resistance to lipid accumulation by enhancing the Akt/GSK3β signaling pathways and modulating the downstream expressions related to lipid metabolism, which indicated that CA might be useful as a natural and promising nonalcoholic fatty liver diseases (NAFLD) modulator
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Cichoric Acid improved hyperglycaemia and restored muscle injury via activating antioxidant response in mld stz induced diabetic mice
Food and Chemical Toxicology, 2017Co-Authors: Di Zhu, Zhigang Liu, Xinglin Zhang, Yajie Niu, Zhijun Diao, Bo Ren, Xuebo LiuAbstract:Abstract Cichoric Acid (CA), extracted from edible plants and vegetables, is a potential natural nutraceutical, with antioxidant and hypoglycaemic biological functions. The objective of this study was to explore the potential underlying molecular mechanisms involved in normalizing diabetes-related changes in hyperglycaemia via pancreas apoptosis and muscle injury induced by multiple low-dose STZ (MLD-STZ) injection in response to dietary supplementation with CA. To induce the MLD-STZ diabetic mice, the C57BL/6J mice were intraperitoneally injected with STZ (50 mg/kg body weight) for consecutive five days. CA (60 mg/kg/d) was supplemented in drinking water for 4 weeks. Compared with control, CA inhibited pancreas apoptosis and adjusted islet function in diabetic mice, leading to an increase in insulin generation and secretion. Moreover, CA regulated mitochondrial biogenesis, glycogen synthesis, and inhibited inflammation via activating antioxidant responses, which contributes to the improvement in athletic ability and diabetic myopathy. In general, CA is a natural food-derived compound with the potential application for regulating glucose homeostasis and improving diabetes and its complications.
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Cichoric Acid regulates the hepatic glucose homeostasis via ampk pathway and activates the antioxidant response in high glucose induced hepatocyte injury
RSC Advances, 2017Co-Authors: Di Zhu, Zhigang Liu, Ni Zhang, Xuelian Zhou, Mengying Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a plant-based nutraceutical, is extracted from Echinacea purpurea and other edible plants and vegetables and exhibits multiple biological functions, including antioxidant and hypoglycemic effects. The objective of this study was to determine the effect of CA on the energy regulation pathways and the antioxidant response system in insulin resistance and diabetes-induced hepatic injury and the underlying molecular mechanisms. The streptozotocin-induced diabetic C57BL/6J mice and glucosamine-induced HepG2 cells were observed to evaluate the hepatic protective effects of CA. Compared to the control, CA (60 mg kg−1 d−1, in drinking water for 4 weeks) inhibited hepatic injury and chronic inflammation in diabetic mice via antioxidant defence and regulated the balance of gluconeogenesis and glycolysis. CA (100 μM) regulated glucose metabolism and activated antioxidant response in glucosamine-induced HepG2 cells. CA increased the phosphorylation of the AMP-activated protein kinase and glycogen synthase kinase-3β and stimulated glycogen synthesis and glucose uptake. CA also activated the Nrf2-Keap1 pathway and increased the antioxidant enzyme expression. CA is a potential natural nutraceutical for regulating hepatic glucose homeostasis and antioxidant response, which improved insulin resistance and hepatic injury in diabetes.
Zhigang Liu - One of the best experts on this subject based on the ideXlab platform.
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Cichoric Acid prevents free fatty Acid induced lipid metabolism disorders via regulating bmal1 in hepg2 cells
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Rui Guo, Yutang Wang, Zhigang Liu, Beita Zhao, Yijie Wang, Yuchen Yan, Wentong Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during the process of CA-attenuated lipid accumulation in hepatocytes. In the current study, CA treatments improved cell morphology changes and hepatic lipid levels, which were triggered by free fatty Acids (2:1, oleate: palmitate) in a dose-dependent way. Besides, CA (200 μM) regulated the circadian rhythm expressions of clock genes and the relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked the p-Akt/Akt pathway to 80.1% ± 1.5% and the p-GSK3β/GSK3β pathway to 64.7% ± 2.8% (p < 0.05). Furthermore, silencing Bmal1 elevated the expressions of FAS and ACC to 122.4% ± 5.6% and 114.9% ± 1.7% in protein levels (p < 0.05) and to 166.5% ± 18.5% and 131.4% ± 5.5% in mRNA levels (p < 0.05). Therefore, our results demonstrated that CA has a Bmal1 resistance to lipid a...
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Cichoric Acid Prevents Free-Fatty-Acid-Induced Lipid Metabolism Disorders via Regulating Bmal1 in HepG2 Cells
2018Co-Authors: Rui Guo, Yutang Wang, Zhigang Liu, Beita Zhao, Yijie Wang, Yuchen Yan, Wentong Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during the process of CA-attenuated lipid accumulation in hepatocytes. In the current study, CA treatments improved cell morphology changes and hepatic lipid levels, which were triggered by free fatty Acids (2:1, oleate: palmitate) in a dose-dependent way. Besides, CA (200 μM) regulated the circadian rhythm expressions of clock genes and the relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked the p-Akt/Akt pathway to 80.1% ± 1.5% and the p-GSK3β/GSK3β pathway to 64.7% ± 2.8% (p < 0.05). Furthermore, silencing Bmal1 elevated the expressions of FAS and ACC to 122.4% ± 5.6% and 114.9% ± 1.7% in protein levels (p < 0.05) and to 166.5% ± 18.5% and 131.4% ± 5.5% in mRNA levels (p < 0.05). Therefore, our results demonstrated that CA has a Bmal1 resistance to lipid accumulation by enhancing the Akt/GSK3β signaling pathways and modulating the downstream expressions related to lipid metabolism, which indicated that CA might be useful as a natural and promising nonalcoholic fatty liver diseases (NAFLD) modulator
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Cichoric Acid improved hyperglycaemia and restored muscle injury via activating antioxidant response in mld stz induced diabetic mice
Food and Chemical Toxicology, 2017Co-Authors: Di Zhu, Zhigang Liu, Xinglin Zhang, Yajie Niu, Zhijun Diao, Bo Ren, Xuebo LiuAbstract:Abstract Cichoric Acid (CA), extracted from edible plants and vegetables, is a potential natural nutraceutical, with antioxidant and hypoglycaemic biological functions. The objective of this study was to explore the potential underlying molecular mechanisms involved in normalizing diabetes-related changes in hyperglycaemia via pancreas apoptosis and muscle injury induced by multiple low-dose STZ (MLD-STZ) injection in response to dietary supplementation with CA. To induce the MLD-STZ diabetic mice, the C57BL/6J mice were intraperitoneally injected with STZ (50 mg/kg body weight) for consecutive five days. CA (60 mg/kg/d) was supplemented in drinking water for 4 weeks. Compared with control, CA inhibited pancreas apoptosis and adjusted islet function in diabetic mice, leading to an increase in insulin generation and secretion. Moreover, CA regulated mitochondrial biogenesis, glycogen synthesis, and inhibited inflammation via activating antioxidant responses, which contributes to the improvement in athletic ability and diabetic myopathy. In general, CA is a natural food-derived compound with the potential application for regulating glucose homeostasis and improving diabetes and its complications.
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Cichoric Acid regulates the hepatic glucose homeostasis via ampk pathway and activates the antioxidant response in high glucose induced hepatocyte injury
RSC Advances, 2017Co-Authors: Di Zhu, Zhigang Liu, Ni Zhang, Xuelian Zhou, Mengying Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a plant-based nutraceutical, is extracted from Echinacea purpurea and other edible plants and vegetables and exhibits multiple biological functions, including antioxidant and hypoglycemic effects. The objective of this study was to determine the effect of CA on the energy regulation pathways and the antioxidant response system in insulin resistance and diabetes-induced hepatic injury and the underlying molecular mechanisms. The streptozotocin-induced diabetic C57BL/6J mice and glucosamine-induced HepG2 cells were observed to evaluate the hepatic protective effects of CA. Compared to the control, CA (60 mg kg−1 d−1, in drinking water for 4 weeks) inhibited hepatic injury and chronic inflammation in diabetic mice via antioxidant defence and regulated the balance of gluconeogenesis and glycolysis. CA (100 μM) regulated glucose metabolism and activated antioxidant response in glucosamine-induced HepG2 cells. CA increased the phosphorylation of the AMP-activated protein kinase and glycogen synthase kinase-3β and stimulated glycogen synthesis and glucose uptake. CA also activated the Nrf2-Keap1 pathway and increased the antioxidant enzyme expression. CA is a potential natural nutraceutical for regulating hepatic glucose homeostasis and antioxidant response, which improved insulin resistance and hepatic injury in diabetes.
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Cichoric Acid reverses insulin resistance and suppresses inflammatory responses in the glucosamine induced hepg2 cells
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Di Zhu, Yutang Wang, Zhigang Liu, Xuebo LiuAbstract:Cichoric Acid, a caffeic Acid derivative found in Echinacea purpurea, basil, and chicory, has been reported to have bioactive effects, such as anti-inflammatory, antioxidant, and preventing insulin resistance. In this study, to explore the effects of CA on regulating insulin resistance and chronic inflammatory responses, the insulin resistance model was constructed by glucosamine in HepG2 cells. CA stimulated glucosamine-mediated glucose uptake by stimulating translocation of the glucose transporter 2. Moreover, the production of reactive oxygen, the expression of COX-2 and iNOS, and the mRNA levels of TNF-α and IL-6 were attenuated. Furthermore, CA was verified to promote glucosamine-mediated glucose uptake and inhibited inflammation through PI3K/Akt, NF-κB, and MAPK signaling pathways in HepG2 cells. These results implied that CA could increase glucose uptake, improve insulin resistance, and attenuate glucosamine-induced inflammation, suggesting that CA is a potential natural nutraceutical with antidiabetic properties and anti-inflammatory effects.
Di Zhu - One of the best experts on this subject based on the ideXlab platform.
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Cichoric Acid improved hyperglycaemia and restored muscle injury via activating antioxidant response in mld stz induced diabetic mice
Food and Chemical Toxicology, 2017Co-Authors: Di Zhu, Zhigang Liu, Xinglin Zhang, Yajie Niu, Zhijun Diao, Bo Ren, Xuebo LiuAbstract:Abstract Cichoric Acid (CA), extracted from edible plants and vegetables, is a potential natural nutraceutical, with antioxidant and hypoglycaemic biological functions. The objective of this study was to explore the potential underlying molecular mechanisms involved in normalizing diabetes-related changes in hyperglycaemia via pancreas apoptosis and muscle injury induced by multiple low-dose STZ (MLD-STZ) injection in response to dietary supplementation with CA. To induce the MLD-STZ diabetic mice, the C57BL/6J mice were intraperitoneally injected with STZ (50 mg/kg body weight) for consecutive five days. CA (60 mg/kg/d) was supplemented in drinking water for 4 weeks. Compared with control, CA inhibited pancreas apoptosis and adjusted islet function in diabetic mice, leading to an increase in insulin generation and secretion. Moreover, CA regulated mitochondrial biogenesis, glycogen synthesis, and inhibited inflammation via activating antioxidant responses, which contributes to the improvement in athletic ability and diabetic myopathy. In general, CA is a natural food-derived compound with the potential application for regulating glucose homeostasis and improving diabetes and its complications.
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Cichoric Acid regulates the hepatic glucose homeostasis via ampk pathway and activates the antioxidant response in high glucose induced hepatocyte injury
RSC Advances, 2017Co-Authors: Di Zhu, Zhigang Liu, Ni Zhang, Xuelian Zhou, Mengying Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a plant-based nutraceutical, is extracted from Echinacea purpurea and other edible plants and vegetables and exhibits multiple biological functions, including antioxidant and hypoglycemic effects. The objective of this study was to determine the effect of CA on the energy regulation pathways and the antioxidant response system in insulin resistance and diabetes-induced hepatic injury and the underlying molecular mechanisms. The streptozotocin-induced diabetic C57BL/6J mice and glucosamine-induced HepG2 cells were observed to evaluate the hepatic protective effects of CA. Compared to the control, CA (60 mg kg−1 d−1, in drinking water for 4 weeks) inhibited hepatic injury and chronic inflammation in diabetic mice via antioxidant defence and regulated the balance of gluconeogenesis and glycolysis. CA (100 μM) regulated glucose metabolism and activated antioxidant response in glucosamine-induced HepG2 cells. CA increased the phosphorylation of the AMP-activated protein kinase and glycogen synthase kinase-3β and stimulated glycogen synthesis and glucose uptake. CA also activated the Nrf2-Keap1 pathway and increased the antioxidant enzyme expression. CA is a potential natural nutraceutical for regulating hepatic glucose homeostasis and antioxidant response, which improved insulin resistance and hepatic injury in diabetes.
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Cichoric Acid reverses insulin resistance and suppresses inflammatory responses in the glucosamine induced hepg2 cells
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Di Zhu, Yutang Wang, Zhigang Liu, Xuebo LiuAbstract:Cichoric Acid, a caffeic Acid derivative found in Echinacea purpurea, basil, and chicory, has been reported to have bioactive effects, such as anti-inflammatory, antioxidant, and preventing insulin resistance. In this study, to explore the effects of CA on regulating insulin resistance and chronic inflammatory responses, the insulin resistance model was constructed by glucosamine in HepG2 cells. CA stimulated glucosamine-mediated glucose uptake by stimulating translocation of the glucose transporter 2. Moreover, the production of reactive oxygen, the expression of COX-2 and iNOS, and the mRNA levels of TNF-α and IL-6 were attenuated. Furthermore, CA was verified to promote glucosamine-mediated glucose uptake and inhibited inflammation through PI3K/Akt, NF-κB, and MAPK signaling pathways in HepG2 cells. These results implied that CA could increase glucose uptake, improve insulin resistance, and attenuate glucosamine-induced inflammation, suggesting that CA is a potential natural nutraceutical with antidiabetic properties and anti-inflammatory effects.
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Cichoric Acid Reverses Insulin Resistance and Suppresses Inflammatory Responses in the Glucosamine-Induced HepG2 Cells
2015Co-Authors: Di Zhu, Yutang Wang, Zhigang Liu, Xuebo LiuAbstract:Cichoric Acid, a caffeic Acid derivative found in Echinacea purpurea, basil, and chicory, has been reported to have bioactive effects, such as anti-inflammatory, antioxidant, and preventing insulin resistance. In this study, to explore the effects of CA on regulating insulin resistance and chronic inflammatory responses, the insulin resistance model was constructed by glucosamine in HepG2 cells. CA stimulated glucosamine-mediated glucose uptake by stimulating translocation of the glucose transporter 2. Moreover, the production of reactive oxygen, the expression of COX-2 and iNOS, and the mRNA levels of TNF-α and IL-6 were attenuated. Furthermore, CA was verified to promote glucosamine-mediated glucose uptake and inhibited inflammation through PI3K/Akt, NF-κB, and MAPK signaling pathways in HepG2 cells. These results implied that CA could increase glucose uptake, improve insulin resistance, and attenuate glucosamine-induced inflammation, suggesting that CA is a potential natural nutraceutical with antidiabetic properties and anti-inflammatory effects
Yutang Wang - One of the best experts on this subject based on the ideXlab platform.
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preservation of Cichoric Acid antioxidant properties loaded in heat treated lactoferrin nanoparticles
Molecules, 2018Co-Authors: Caicai Zhao, Liping Wei, Fuguo Liu, Min Zhang, Xuebo Liu, Yutang WangAbstract:In the current research, a new Cichoric Acid (CA) encapsulation system was investigated. The optimal condition for the formation of lactoferrin-Cichoric Acid nanoparticles (LF-CA NPs) was determined by controlling the solution pH, the thermal treatment conditions, and the concentration of CA. Fluorescence indicated that the electrostatic force and the hydrophobic force were the main forces in the formation of LF-CA NPs. LF-CA NPs prepared under different conditions were spherical in shape with smaller particle sizes and good zeta potential demonstrating good colloidal stability. Especially, the prepared particle size of the LF-CA NPs at pH 7 and 95 °C was about 67.20 ± 1.86 nm. The circular dichroism (CD) and the Fourier transform infrared spectroscopy (FTIR) results showed that the combination of LF (lactoferrin) and CA affected the secondary structure of the LF. The differential scanning calorimetry (DSC) results indicated that the addition of CA increased the thermal stability of LF. In vitro antioxidant experiments confirmed the antioxidant capacity of LF-CA NPs was better than CA. CA was successfully encapsulated into LF NPs with high encapsulated efficiency (97.87–99.87%) by high performance liquid chromatography (HPLC). These results showed that LF could be used as the wall material of CA with excellent nature.
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Cichoric Acid prevents free fatty Acid induced lipid metabolism disorders via regulating bmal1 in hepg2 cells
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Rui Guo, Yutang Wang, Zhigang Liu, Beita Zhao, Yijie Wang, Yuchen Yan, Wentong Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during the process of CA-attenuated lipid accumulation in hepatocytes. In the current study, CA treatments improved cell morphology changes and hepatic lipid levels, which were triggered by free fatty Acids (2:1, oleate: palmitate) in a dose-dependent way. Besides, CA (200 μM) regulated the circadian rhythm expressions of clock genes and the relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked the p-Akt/Akt pathway to 80.1% ± 1.5% and the p-GSK3β/GSK3β pathway to 64.7% ± 2.8% (p < 0.05). Furthermore, silencing Bmal1 elevated the expressions of FAS and ACC to 122.4% ± 5.6% and 114.9% ± 1.7% in protein levels (p < 0.05) and to 166.5% ± 18.5% and 131.4% ± 5.5% in mRNA levels (p < 0.05). Therefore, our results demonstrated that CA has a Bmal1 resistance to lipid a...
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Cichoric Acid Prevents Free-Fatty-Acid-Induced Lipid Metabolism Disorders via Regulating Bmal1 in HepG2 Cells
2018Co-Authors: Rui Guo, Yutang Wang, Zhigang Liu, Beita Zhao, Yijie Wang, Yuchen Yan, Wentong Zhang, Xuebo LiuAbstract:Cichoric Acid (CA), a polyphenol component from Echinacea purpurea, exhibits preventive effects on liver lipid-metabolism disorders in obesity. This research aimed to determine the role of circadian rhythm signaling during the process of CA-attenuated lipid accumulation in hepatocytes. In the current study, CA treatments improved cell morphology changes and hepatic lipid levels, which were triggered by free fatty Acids (2:1, oleate: palmitate) in a dose-dependent way. Besides, CA (200 μM) regulated the circadian rhythm expressions of clock genes and the relatively shallow daily oscillations. Moreover, silencing Bmal1 significantly blocked the p-Akt/Akt pathway to 80.1% ± 1.5% and the p-GSK3β/GSK3β pathway to 64.7% ± 2.8% (p < 0.05). Furthermore, silencing Bmal1 elevated the expressions of FAS and ACC to 122.4% ± 5.6% and 114.9% ± 1.7% in protein levels (p < 0.05) and to 166.5% ± 18.5% and 131.4% ± 5.5% in mRNA levels (p < 0.05). Therefore, our results demonstrated that CA has a Bmal1 resistance to lipid accumulation by enhancing the Akt/GSK3β signaling pathways and modulating the downstream expressions related to lipid metabolism, which indicated that CA might be useful as a natural and promising nonalcoholic fatty liver diseases (NAFLD) modulator
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Cichoric Acid reverses insulin resistance and suppresses inflammatory responses in the glucosamine induced hepg2 cells
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Di Zhu, Yutang Wang, Zhigang Liu, Xuebo LiuAbstract:Cichoric Acid, a caffeic Acid derivative found in Echinacea purpurea, basil, and chicory, has been reported to have bioactive effects, such as anti-inflammatory, antioxidant, and preventing insulin resistance. In this study, to explore the effects of CA on regulating insulin resistance and chronic inflammatory responses, the insulin resistance model was constructed by glucosamine in HepG2 cells. CA stimulated glucosamine-mediated glucose uptake by stimulating translocation of the glucose transporter 2. Moreover, the production of reactive oxygen, the expression of COX-2 and iNOS, and the mRNA levels of TNF-α and IL-6 were attenuated. Furthermore, CA was verified to promote glucosamine-mediated glucose uptake and inhibited inflammation through PI3K/Akt, NF-κB, and MAPK signaling pathways in HepG2 cells. These results implied that CA could increase glucose uptake, improve insulin resistance, and attenuate glucosamine-induced inflammation, suggesting that CA is a potential natural nutraceutical with antidiabetic properties and anti-inflammatory effects.
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Cichoric Acid Reverses Insulin Resistance and Suppresses Inflammatory Responses in the Glucosamine-Induced HepG2 Cells
2015Co-Authors: Di Zhu, Yutang Wang, Zhigang Liu, Xuebo LiuAbstract:Cichoric Acid, a caffeic Acid derivative found in Echinacea purpurea, basil, and chicory, has been reported to have bioactive effects, such as anti-inflammatory, antioxidant, and preventing insulin resistance. In this study, to explore the effects of CA on regulating insulin resistance and chronic inflammatory responses, the insulin resistance model was constructed by glucosamine in HepG2 cells. CA stimulated glucosamine-mediated glucose uptake by stimulating translocation of the glucose transporter 2. Moreover, the production of reactive oxygen, the expression of COX-2 and iNOS, and the mRNA levels of TNF-α and IL-6 were attenuated. Furthermore, CA was verified to promote glucosamine-mediated glucose uptake and inhibited inflammation through PI3K/Akt, NF-κB, and MAPK signaling pathways in HepG2 cells. These results implied that CA could increase glucose uptake, improve insulin resistance, and attenuate glucosamine-induced inflammation, suggesting that CA is a potential natural nutraceutical with antidiabetic properties and anti-inflammatory effects
Chunzhao Liu - One of the best experts on this subject based on the ideXlab platform.
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improving root growth and Cichoric Acid derivatives production in hairy root culture of echinacea purpurea by ultrasound treatment
Biochemical Engineering Journal, 2012Co-Authors: Rui Liu, Liyang Sun, Chunzhao LiuAbstract:An efficient ultrasound-stimulation strategy was developed for improving the hairy root growth and caffeic Acid derivatives (CADs) biosynthesis in the hairy root cultures of Echinacea purpurea L. The 15-day-old hairy roots stimulated every 5 days by ultrasound for 6 min produced the highest amount of CADs after 30 days of culture among all ultrasound treatment experiments. The obvious increase of CADs production in E. purpurea hairy roots stimulated by ultrasound was related to the increase of both rolB-regulated endogenous indole-3-acetic Acid biosynthesis and phenylalanine ammonium lyase (PAL) activity. These results provided a basis for understanding of improving growth and secondary metabolism in the process of hairy root culture stimulated by ultrasound. (C) 2011 Elsevier B.V. All rights reserved.
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Cichoric Acid production from hairy root cultures of echinacea purpurea grown in a modified airlift bioreactor
Journal of Chemical Technology & Biotechnology, 2009Co-Authors: Bilal Haider Abbasi, Rui Liu, Praveen K Saxena, Chunzhao LiuAbstract:BACKGROUND: Hairy root cultures of Echinacea offer great potential for the production of valuable Cichoric Acid, but scale-up of the culture in the bioreactor represents a big challenge. Therefore, there is great interest in developing a suitable bioreactor for hairy root culture of Echinacea and novel bioprocessing strategies for intensifying Cichoric Acid production.
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light enhanced caffeic Acid derivatives biosynthesis in hairy root cultures of echinacea purpurea
Plant Cell Reports, 2007Co-Authors: Bilal Haider Abbasi, Praveen K Saxena, Chunzhao Liu, Chunlong Tian, Susan J MurchAbstract:Light plays an important role in almost all plant developmental processes and provides the fundamental building blocks for growth, development, primary and secondary metabolism. The effects of light on growth rate and caffeic Acid derivative (CADs) biosynthesis in hairy root cultures of Echinacea purpurea (Moench) were assessed. Light-grown hairy roots accumulated increased levels of anthocyanins, which became visible in outer cell layer of the cortex as a ring of purple color. The light-grown root cultures also had radially thickened morphology compared with the dark-grown controls. The growth rate and cell viability of the hairy root cultures in light did not show obvious difference in comparison with those in dark. However, biosynthesis of CADs including Cichoric Acid, caftaric Acid, chlorogenic Acid and caffeic Acid was significantly increased in hairy root cultures grown in the light. The enhanced accumulation of CADs and anthocyanins in E. purpurea hairy root cultures was correlated to an observed light-stimulated activity of phenylalanine ammonium lyase (PAL).