The Experts below are selected from a list of 201 Experts worldwide ranked by ideXlab platform

Pooria Gill - One of the best experts on this subject based on the ideXlab platform.

  • Circular Dichroism techniques: Biomolecular and nanostructural analyses- A review
    Chemical Biology and Drug Design, 2009
    Co-Authors: Bijan Ranjbar, Pooria Gill
    Abstract:

    This paper reviews the best known techniques using Circular Dichroism spectroscopy such as conventional Circular Dichroism (i.e. electronic Circular Dichroism), magnetic Circular Dichroisms (magnetic vibrational Circular Dichroism, x-ray magnetic Circular Dichroism), fluorescence detected Circular Dichroism, near-infrared Circular Dichroism, vibrational Circular Dichroism, Fourier transform infrared Circular Dichroism, high pressure liquid chromatography Circular Dichroism, stopped-flow Circular Dichroism, and synchrotron radiation Circular Dichroism. Also, we have described here the most important applications of Circular Dichroism spectroscopy in structural biochemistry and nanoscience.

Robert W Woody - One of the best experts on this subject based on the ideXlab platform.

  • 4 Circular Dichroism
    Methods in Enzymology, 1995
    Co-Authors: Robert W Woody
    Abstract:

    Publisher Summary Circular Dichroism (CD) is a spectroscopic method which depends on the fact that certain molecules interact differently with right and left Circularly polarized light. Circularly polarized light is chiral—that is, it occurs in two nonsuperimposable forms that are mirror images of one another. To discriminate between the two chiral forms of light, a molecule must be chiral, including the vast majority of biological molecules. A method that can discern the subtle differences between non superimposable mirror image molecules (enantiomers) must be highly sensitive to the three-dimensional features of molecules—that is, to conformation. Binding of ligands or protein-protein and protein–DNA interactions can also alter the Circular Dichroism spectrum of the protein and/or nucleic acid. These changes in CD can be used to determine equilibrium constants, and they can also provide evidence for conformational changes. Thus, CD can provide information about the secondary structure of proteins and nucleic acids and about the binding of ligands to these types of macromolecule.

  • Circular Dichroism principles and applications
    1994
    Co-Authors: Nina Berova, 香爾 中西, Robert W Woody
    Abstract:

    PARTIAL TABLE OF CONTENTS: Circular Dichroism: An Introduction (G. Snatzke). From Pasteur to Parity Nonconservation: Theories of the Origin of Molecular Chirality (S. Mason). Theoretical Approach to Electronic Optical Activity (A. Koslowski, et al.). Vibrational Optical Acivity Theory (L. Nafie & T. Freedman). Fast Time-Resolved Circular Dichroism Measurements (D. Kliger & J. Lewis). The Octant Rule (D. Lightner). Circular Dichroism and Chirality of Dienes (J. Gawroski). Exciton Chirality Method: Principles and Applications (N. Berova & K. Nakanishi). A Model for How Polymers Amplify Chirality (M. Green). Induced CD or Polymers (E. Yashima & Y. Okamoto). Application of CD to the Study of Some Cholesteric Mesophase (G. Gottarelli & G. Spada). Circular Dichroism of Inorganic Complexes: Interpretation and Applications (R. Kuroda). Circular Dichroism of Peptides and Proteins (N. Sreerama & R. Woody). Peptide and Protein Conformational Studies with Vibrational Circular Dichroism and Related Spectroscopies (T. Keiderling). HPLC-CD: Stereochemical Analysis at Work (P. Salvadori, et al.). Applications of Chiroptical Spectroscopy in the Characterization of Compounds Having Pharmaceutical Importance (H. Brittain). Index.

Bijan Ranjbar - One of the best experts on this subject based on the ideXlab platform.

  • Circular Dichroism techniques: Biomolecular and nanostructural analyses- A review
    Chemical Biology and Drug Design, 2009
    Co-Authors: Bijan Ranjbar, Pooria Gill
    Abstract:

    This paper reviews the best known techniques using Circular Dichroism spectroscopy such as conventional Circular Dichroism (i.e. electronic Circular Dichroism), magnetic Circular Dichroisms (magnetic vibrational Circular Dichroism, x-ray magnetic Circular Dichroism), fluorescence detected Circular Dichroism, near-infrared Circular Dichroism, vibrational Circular Dichroism, Fourier transform infrared Circular Dichroism, high pressure liquid chromatography Circular Dichroism, stopped-flow Circular Dichroism, and synchrotron radiation Circular Dichroism. Also, we have described here the most important applications of Circular Dichroism spectroscopy in structural biochemistry and nanoscience.

Valerie Gabelica - One of the best experts on this subject based on the ideXlab platform.

  • mass resolved electronic Circular Dichroism ion spectroscopy
    Science, 2020
    Co-Authors: Steven Daly, Frederic Rosu, Valerie Gabelica
    Abstract:

    DNA and proteins are chiral: Their three-dimensional structures cannot be superimposed with their mirror images. Circular Dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case of mixtures. We recorded the electronic Circular Dichroism spectra of DNA helices separated in a mass spectrometer. We studied guanine-rich strands having various secondary structures, electrosprayed them as negative ions, irradiated them with an ultraviolet nanosecond optical parametric oscillator laser, and measured the difference in electron photodetachment efficiency between left and right Circularly polarized light. The reconstructed Circular Dichroism ion spectra resembled those of their solution-phase counterparts, thereby allowing us to assign the DNA helical topology. The ability to measure Circular Dichroism directly on biomolecular ions expands the capabilities of mass spectrometry for structural analysis.

  • mass resolved electronic Circular Dichroism ion spectroscopy
    arXiv: Biomolecules, 2020
    Co-Authors: Steven Daly, Frederic Rosu, Valerie Gabelica
    Abstract:

    DNA and proteins are chiral: their three-dimensional structure cannot be superimposed with its mirror image. Circular Dichroism spectroscopy is widely used to characterize chiral compounds, but data interpretation is difficult in the case of mixtures. We recorded for the first time the electronic Circular Dichroism spectra of DNA helices separated in a mass spectrometer. We electrosprayed guanine-rich strands having various secondary structures as negative ions, irradiated them with a laser, and measured the difference in electron photodetachment efficiency between left and right Circularly polarized light. The reconstructed Circular Dichroism ion spectra resemble the solution ones, thereby allowing us to assign the DNA helical topology. The ability to measure Circular Dichroism directly on biomolecular ions expands the capabilities of mass spectrometry for structural analysis.

Robert W. Janes - One of the best experts on this subject based on the ideXlab platform.

  • Modern techniques for Circular Dichroism and synchrotron radiation Circular Dichroism spectroscopy: 1
    2020
    Co-Authors: Bonnie A. Wallace, Robert W. Janes
    Abstract:

    This timely book provides a comprehensive and up-to-date account of Circular Dichroism (CD) spectroscopy and its application to structural biology. In particular, the newer methods of synchrotron radiation Circular Dichroism (SRCD) and linear Dichroism (LD) are fully covered. Use of SRCD allows data between 190 and 160nm to be measured, where additional information content is obtained plus the ability to characterise biomolecules in diverse environments. The method has value in protein fold recognition and potential for use in structural genomics.

  • PCDDB: the Protein Circular Dichroism Data Bank, a repository for Circular Dichroism spectral and metadata
    Nucleic Acids Research, 2010
    Co-Authors: Lee Whitmore, Robert W. Janes, Benjamin Woollett, Andrew J. Miles, D. P. Klose, Bonnie A. Wallace
    Abstract:

    The Protein Circular Dichroism Data Bank (PCDDB) is a public repository that archives and freely distributes Circular Dichroism (CD) and synchrotron radiation CD (SRCD) spectral data and their associated experimental metadata. All entries undergo validation and curation procedures to ensure completeness, consistency and quality of the data included. A web-based interface enables users to browse and query sample types, sample conditions, experimental parameters and provides spectra in both graphical display format and as downloadable text files. The entries are linked, when appropriate, to primary sequence (UniProt) and structural (PDB) databases, as well as to secondary databases such as the Enzyme Commission functional classification database and the CATH fold classification database, as well as to literature citations. The PCDDB is available at: http://pcddb.cryst.bbk.ac.uk.

  • The Protein Circular Dichroism Data Bank, A Web-Based Site for Access to Circular Dichroism Spectroscopic Data
    Structure, 2010
    Co-Authors: Lee Whitmore, Robert W. Janes, Benjamin Woollett, Andrew J. Miles, Bonnie A. Wallace
    Abstract:

    Summary The Protein Circular Dichroism Data Bank (PCDDB) is a newly released resource for structural biology. It is a web-accessible (http://pcddb.cryst.bbk.ac.uk) data bank for Circular Dichroism (CD) and synchrotron radiation Circular Dichroism (SRCD) spectra and their associated experimental and secondary metadata, with links to protein sequence and structure data banks. It is designed to provide a public repository for CD spectroscopic data on macromolecules, to parallel the Protein Data Bank (PDB) for crystallographic, electron microscopic, and nuclear magnetic resonance spectroscopic data. Similarly to the PDB, it includes validation checking procedures to ensure good practice and the integrity of the deposited data. This paper reports on the first public release of the PCDDB, which provides access to spectral data that comprise standard reference datasets.