The Experts below are selected from a list of 4128 Experts worldwide ranked by ideXlab platform

Eladio Hellín - One of the best experts on this subject based on the ideXlab platform.

  • Cytological changes related with salt tolerance in embryogenic callus of Citrus Limon
    Plant Cell Tissue and Organ Culture, 1994
    Co-Authors: Abel Piqueras, Enrique Olmos, Eladio Hellín
    Abstract:

    Electron microscopy observations of salt-tolerant embrogenic calli of Citrus Limon [(L.) Burm. f.] showed several changes in cell ultrastructure when compared with control calli. Both types of calli comprised clusters of meristematic cells, but salt-tolerant calli had several structural differences: thick cell walls, ring-shaped mitochondria, an increased content of lipid bodies, microbodies and parallel accumulation of rough endoplasmatic reticulum. These structural features seem to be related with salt tolerance in Citrus Limon cells.

Farhan Haidar Fazlur Rahman - One of the best experts on this subject based on the ideXlab platform.

  • In vitro Antimicrobial Potency of Lemon Fruit (Citrus Limon) Extract on Salmonella typhi
    JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga, 2020
    Co-Authors: Farhan Haidar Fazlur Rahman, Lindawati Alimsardjono, Sunarni Zakaria
    Abstract:

    Introduction: This study aimed to evaluate minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lemon fruit (Citrus Limon) extract in inhibiting Salmonella typhi growth in vitro.Methods: This research was categorized as a laboratory experimental study. Lemon fruit (Citrus Limon) extract was prepared with concentration as follows: 100.000 ppm, 50.000 ppm, 25.000 ppm, 12.500 ppm, 6.250 ppm, 3.125 ppm, 1.562 ppm, 781 ppm, and 390 ppm. Dilution tests with Mueller-Hinton broth medium were performed to determine the MIC. After 24 hours of incubation, isolated Salmonella typhi inside the tube was inoculated back in MacConkey agar plate medium to determine the MBC. Replications were conducted 3 times according to Federer’s formula.Results: MIC of lemon fruit (Citrus Limon) extract to Salmonella typhi was determined at 3.125 ppm. Meanwhile, MBC was determined at 6.250 ppm.Conclusion: This study showed the potential antimicrobial effect of lemon fruit (Citrus Limon) extract against Salmonella typhi in-vitro. Further studies are still needed to determine its efficacy and safety in vivo and also its full antibacterial spectrum. 

  • UJI KEPEKAAN Salmonella typhi TERHADAP EKSTRAK BUAH LEMON (Citrus Limon)
    2017
    Co-Authors: Farhan Haidar Fazlur Rahman
    Abstract:

    The incidence of typhoid fever in Indonesia is high and alarming (148.7 per 100,000 persons/year). More than 90% of the world morbidity by typhoid occurs in Asia alone. Lemon fruit (Citrus Limon) is known to have antimicrobial effect. This research’s aim is to find the potency of antimicrobial effect of lemon fruit (Citrus Limon) extract towards Salmonella typhi. This research was categorized as true experimental method. Salmonella typhi specimens were obtained from Microbiology Laboratory Faculty of Medicine Universitas Airlangga, Surabaya. Lemon fruit extract (Citrus Limon) was prepared with concentration as follows: 100.000 ppm, 50.000 ppm, 25.000 ppm, 12.500 ppm, 6.250 ppm, 3.125 ppm, 1.562 ppm, 781 ppm, dan 390 ppm. Dilution test with Mueller-Hinton broth medium were done to determine the minimum inhibitory concentration (MIC). After 24 hours of incubation, isolated bacteria inside the tube was planted back in MacConkey agar plate medium to determine the minimum bactericidal concentration (MBC). Replication of this experiment were conducted 3 times according to Federer’s formula. Minimum inhibitory concentration (MIC) of lemon fruit extract (Citrus Limon) to Salmonella typhi was determined at 3.125 ppm. Meanwhile, minimum bactericidal concentration (MBC) of lemon fruit extract (Citrus Limon) to Salmonella typhi was determined at 6.250 ppm. Further research is needed to determine the therapeutic effects and side effects of lemon fruit (Citrus Limon) extract against Salmonella typhi in vivo. Similar studies with a wider range of bacteria is also needed to know the antibacterial spectrum of the lemon fruit (Citrus Limon) extract.

Azra Riaz - One of the best experts on this subject based on the ideXlab platform.

  • Behavioral effects of Citrus Limon in rats
    Metabolic brain disease, 2014
    Co-Authors: Rafeeq Alam Khan, Azra Riaz
    Abstract:

    Anxiety and depression are increasing worldwide, however these disorders may be managed by making healthier changes is dietary pattern, since there are evidences that diet rich in antioxidants and vitamins help reduce anxiety and depression. Hence present study was designed to evaluate the behavioral effects of Citrus Limon in rats at three different doses i.e. 0.2, 0.4 and 0.6 ml/kg considered as low, moderate and high doses. Anxiolytic and antidepressant activities were specifically assessed twice during 15 days using open field test, elevated plus maze and forced swimming test. In open field test C. Limon, revealed increase in distance travelled, number of central entries and number of rearing’s at moderate dose, while in the elevated plus maze, number of open arm entries were found to be increased. Whereas in forced swimming test, there was decrease in duration of immobility and increase in duration of climbing. Thus results of present study suggest that C. Limon at moderate dose have anxiolytic effect.

  • Memory boosting effect of Citrus Limon, Pomegranate and their combinations.
    Pakistan journal of pharmaceutical sciences, 2014
    Co-Authors: Azra Riaz, Rafeeq Alam Khan, Hussein Algahtani
    Abstract:

    Memory is greatly influenced by factors like food, stress and quality of sleep, hence present study was designed to evaluate the effect of Citrus Limon and Pomegranate juices on memory of mice using Harvard Panlab Passive Avoidance response apparatus controlled through LE2708 Programmer. Passive avoidance is fear-motivated tests used to assess short or long-term memory of small animals, which measures latency to enter into the black compartment. Animals at MCLD showed highly significant and significant increase in latency to enter into the black compartment after 3 and 24 hours respectively than control, animals at HCLD showed significant increase in latency only after 3hours. Animals both at low and moderate doses of pomegranate showed significant increase in test latency after 3 hours, while animals at high dose showed highly significant and significant increase in latency after 3 and 24 hours respectively. There was highly significant and significant increase in latency in animals at CPJ-1combination after 3 and 24 hours respectively; however animals received CPJ-2 combination showed significant increase in latency only after 3 hours as compare to control. These results suggest that Citrus Limon and Pomegranate has phytochemicals and essential nutrients which boost memory, particularly short term memory. Hence it may be concluded that flavonoids in these juices may be responsible for memory enhancing effects and a synergistic effect is observed by CPJ-1 and CPJ-2 combinations.

  • In vitro/in vivo effect of Citrus Limon (L. Burm. f.) juice on blood parameters, coagulation and anticoagulation factors in rabbits.
    Pakistan journal of pharmaceutical sciences, 2014
    Co-Authors: Azra Riaz, Rafeeq Alam Khan, Talat Mirza, Tazeen Mustansir, Mansoor Ahmed
    Abstract:

    The genus Citrus of the family Rutaceae includes many species e.g. Citrus indica, Citrus aurantifolia and Citrus Limon, among which Citrus Limon L. Burm. f. has been reported to have highest antimicrobial activity. It is used as antidote against certain venom, due to its platelet inhibitory effect and also reported to have hypocholesterolemic effect. However its anticoagulant and thrombolytic effect were not been investigated, hence a prospective in-vitro/in-vivo study was designed to determine the effect of Citrus Limon on blood parameters, coagulation and anticoagulation factors. In- vitro tests revealed highly significant increase in thrombin time and activated partial thromboplastin time by Citrus Limon, whereas fibrinogen concentration was significantly reduced in comparison to control, however prothrombin time was not affected significantly. In-vivo testing of Citrus Limon was done at three different doses i.e. 0.2ml/kg, 0.4ml/kg and 0.6ml/kg in healthy rabbits. Significant changes were observed in hematological parameters such as erythrocytes, hemoglobin and mean corpuscular hemoglobin concentration. Bleeding time and thrombin time was significantly prolonged and there was increase in protein C and thrombin antithrombin complex levels. These results may be due to inactivation of thrombin because it significantly decreases fibrinogen concentration and inhibit platelet aggregation. Citrus Limon showed maximal anticoagulant effect at 0.4ml/kg, which suggest that Citrus Limon possesses an anti- thrombin component and could prevent thrombosis playing a cardio protective role.

  • Glycemic Response of Citrus Limon, Pomegranate and Their Combinations in Alloxan- Induced Diabetic Rats
    2013
    Co-Authors: Azra Riaz, Rafeeq Alam Khan, Mansoor Ahmed
    Abstract:

    Present study was performed to investigate the effect of Citrus Limon and Pomegranate juices alone and their combinations on blood glucose and plasma insulin levels in alloxan induced diabetic rats as compared to diabetic control. Decrease in blood glucose level by Citrus Limon was highly significant in dose dependant manner but there was highly significant increase in plasma insulin levels at moderate dose and significant increase at high dose. However Pomegranate showed highly significant decrease in blood glucose, in inverse dose dependant manner i.e. maximum effect was observed at low dose. Whereas there was highly significant increase in plasma insulin level at all three doses. Whereas combination dose group CPJ-1 (0.4+5ml/kg) showed highly significant response on blood glucose and plasma insulin, which was maximum effect obtained among all treated groups. These results suggest that Citrus Limon and Pomegranate has phytochemical and other essential nutrients which have reduced blood glucose levels, that works synergistically in combination. So it may be concluded that the response of Citrus Limon and pomegranate on blood glucose may be due to presence of flavonoids in these juices and a synergistic effect is observed by their combination.

Luz M. Pérez - One of the best experts on this subject based on the ideXlab platform.

  • Participation of the phosphoinositide metabolism in the hypersensitive response of Citrus Limon against Alternaria alternata.
    Biological research, 2001
    Co-Authors: Ximena Ortega, Luz M. Pérez
    Abstract:

    Lemon seedlings inoculated with Alternaria alternata develop a hypersensitive response (HR) that includes the induction of Phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone. The signal transduction pathway involved in the development of this response is unknown. We used several inhibitors of the Phosphoinositide (PI) animal system to study a possible role of Inositol-1,4,5-triphosphate (IP3) in the transduction of the fungal conidia signal in Citrus Limon. The HR was only partially inhibited by EGTA, suggesting that not only external but internal calcium as well are necessary for a complete development of the HR. In this plant system, Alternaria alternata induced an early accumulation of the second messenger IP3. When lemon seedlings were watered long term with LiCl, an inhibitor of the phosphoinositide cycle, the IP3 production was reduced, and the LiCl-watered plants could neither induce PAL nor synthesize scoparone in response to fungal conidia. Furthermore, neomycin, a Phospholipase C (PLC, E. C. 3.1.4.3) inhibitor, also inhibited PAL induction and scoparone synthesis in response to A. alternata. These results suggest that IP3 could be involved in the signal transduction pathway for the development of the HR of Citrus Limon against A. alternata.

Abel Piqueras - One of the best experts on this subject based on the ideXlab platform.

  • Cytological changes related with salt tolerance in embryogenic callus of Citrus Limon
    Plant Cell Tissue and Organ Culture, 1994
    Co-Authors: Abel Piqueras, Enrique Olmos, Eladio Hellín
    Abstract:

    Electron microscopy observations of salt-tolerant embrogenic calli of Citrus Limon [(L.) Burm. f.] showed several changes in cell ultrastructure when compared with control calli. Both types of calli comprised clusters of meristematic cells, but salt-tolerant calli had several structural differences: thick cell walls, ring-shaped mitochondria, an increased content of lipid bodies, microbodies and parallel accumulation of rough endoplasmatic reticulum. These structural features seem to be related with salt tolerance in Citrus Limon cells.