The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
G. S. De Hoog - One of the best experts on this subject based on the ideXlab platform.
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Combination of Amphotericin B and Terbinafine against Melanized Fungi Associated with Chromoblastomycosis
Antimicrobial Agents and Chemotherapy, 2018Co-Authors: Shuwen Deng, G. S. De Hoog, L. Yang, Roxana G. Vitale, Haleh Rafati, M. Seyedmousavi, Ali Tolooe, Wanqing LiaoAbstract:Our in vitro studies showed that a combination of amphotericin B and terbinafine had synergistic effects against the majority of melanized fungi associated with chromoblastomycosis (CBM) and similar infections, including those with Cladophialophora carrionii , Cladophialophora arxii , Exophiala dermatitidis , Exophiala spinifera , Fonsecaea monophora , Fonsecaea nubica , Fonsecaea pedrosoi , and Phialophora verrucosa. This drug combination could provide an option for the treatment of severe or unresponsive cases of CBM, particularly in cases due to species of Fonsecaea and Cladophialophora .
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Cladophialophora abundans a novel species of chaetothyriales isolated from the natural environment
Mycological Progress, 2014Co-Authors: Hamid Badali, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, G. S. De Hoog, Peiying Feng, J B Stielow, Walter A Boeger, Vânia Aparecida VicenteAbstract:A novel species of Cladophialophora is described from the natural environment of the mangrove land crab (Ucides cordatus) along the coast, and from plant sources in Brazil. It is morphologically very similar to Cladophialophora bantiana, though with moderately melanized conidia produced in strongly coherent and poorly branched chains which arise directly from undifferentiated hyphae, but deviates unambiguously on the basis of molecular phylogeny. The species has a maximum growth temperature of 36 °C and seems to be a strict saprobe.
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Use of Rolling Circle Amplification to Rapidly Identify Species of Cladophialophora Potentially Causing Human Infection
Mycopathologia, 2013Co-Authors: Hossein Hamzehei, G. S. De Hoog, Seyed Amir Yazdanparast, Mehrnaz Mohammad Davoudi, Sadegh Khodavaisy, Mitra Golehkheyli, Saham Ansari, Hamid BadaliAbstract:The genus Cladophialophora comprises etiologic agents of disease in immunocompetent patients, ranging from mild cutaneous colonization to cerebral encephalitis, in addition to saprobic species. Due to the high degree of phenotypic similarity between closely related species of the genus, identification problems are imminent. In the present study, we described rapid and sensitive rolling circle amplification (RCA) method based on species-specific padlock probes targeted for the internal transcribed spacer regions of rDNA. ITS regions of 12 Cladophialophora species were sequenced, and subsequently, 10 specific padlock probes were designed for the detection of single nucleotide polymorphisms. The majority of circularizable padlock probes were designed based on single nucleotide polymorphisms (SNPs), while for C. bantiana , C. immunda and C. devriesii were characterized by two or more nucleotides. Individual species-specific probes correctly identified in all ten Cladophialophora species correctly by visualization on 1.2 % agarose gels used to verify specificity of probe-template binding; no cross-reactivity was observed. Simplicity, sensitivity, robustness and low costs provide RCA a distinct place among isothermal techniques for DNA diagnostics. However, restriction and specificity and sensitivity should be lowered and increased, respectively, to be useful for a wide variety of clinical applications.
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in vitro antifungal susceptibility of Cladophialophora carrionii an agent of human chromoblastomycosis
Antimicrobial Agents and Chemotherapy, 2013Co-Authors: Hamid Badali, Jacques F. Meis, Shuwen Deng, Mohammad Javad Najafzadeh, G. S. De Hoog, L. Yang, B Pan, Ilse Curfsbreuker, Wanqing LiaoAbstract:A global collection of Cladophialophora carrionii strains (n = 81) was tested against nine antifungal drugs. MIC90s of all strains were as follows in increasing order: itraconazole and posaconazole, 0.063 μg/ml; terbinafine, 0.125 μg/ml; isavuconazole and voriconazole, 0.25 μg/ml; caspofungin, 2 μg/ml; micafungin, 4 μg/ml; amphotericin B, 8 μg/ml; and fluconazole, 64 μg/ml.
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toenail infection by Cladophialophora boppii
Medical Mycology, 2011Co-Authors: Jochen Brasch, S Dressel, K Mullerwening, R Hugel, D Von Bremen, G. S. De HoogAbstract:Cladophialophora boppii is a black yeast-like fungus that up to now has been only rarely described as a cause of human infection and whose role as a pathogen was not established despite its repeated isolation and genetic identification in these reports. Here we report the first case of a verified toenail infection caused by this fungus in a woman without any systemic disease or evidence of immunodeficiency. Identical dark molds were isolated from the same toenail at three points of time. Species identification was performed by scrutinizing the isolates morphologic, physiologic and genetic characteristics which resulted in their identification as Cladophialophora boppii. Oral treatment with terbinafin plus topical ciclopiroxolamine was effective.
Shuwen Deng - One of the best experts on this subject based on the ideXlab platform.
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Combination of Amphotericin B and Terbinafine against Melanized Fungi Associated with Chromoblastomycosis
Antimicrobial Agents and Chemotherapy, 2018Co-Authors: Shuwen Deng, G. S. De Hoog, L. Yang, Roxana G. Vitale, Haleh Rafati, M. Seyedmousavi, Ali Tolooe, Wanqing LiaoAbstract:Our in vitro studies showed that a combination of amphotericin B and terbinafine had synergistic effects against the majority of melanized fungi associated with chromoblastomycosis (CBM) and similar infections, including those with Cladophialophora carrionii , Cladophialophora arxii , Exophiala dermatitidis , Exophiala spinifera , Fonsecaea monophora , Fonsecaea nubica , Fonsecaea pedrosoi , and Phialophora verrucosa. This drug combination could provide an option for the treatment of severe or unresponsive cases of CBM, particularly in cases due to species of Fonsecaea and Cladophialophora .
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Genetic differentiation among populations occurring on different continents of Cladophialophora carrionii based on concatenated ITS-BT2 sequence data, Fst indices for Cladophialophora carrionii (n = 67).
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Fst indices interpretion:0–0.05: little genetic differentiation; 0.05–0.15: moderate genetic differentiation0.15–0.25: great genetic differentiation; > 0.25: very great genetic differentiation (isolation)Genetic differentiation among populations occurring on different continents of Cladophialophora carrionii based on concatenated ITS-BT2 sequence data, Fst indices for Cladophialophora carrionii (n = 67).
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Split decomposition network of Cladophialophora carrionii constructed with Splitstree (S1 Text).
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Split decomposition network of Cladophialophora carrionii constructed with Splitstree (S1 Text).
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The population structure of Cladophialophora carrionii and Fonsecaea spp. with Q-hat order.
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Structure 2.3.4 used to establish the population structure of global isolates of C. carrionii isolates and Fonsecaea spp., Cladophialophora carrionii K = 4, admixture model, allele frequencies correlated; Fonsecaea species K = 3, admixture model, allele frequencies correlated. In the Structure display the color between parentheses depict the geographical origin of the haplotype group.
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Neutrality test of Cladophialophora carrionii and Fonsecaea spp. based on concatenated ITS-BT2 data.
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:D* Excess of low-frequency variants, as a result from population expansion, weak negative selection or positive selectionF* Excess of intermediate-frequency alleles, as a result from population bottlenecks, structure and/or balancing selectionNeutrality test of Cladophialophora carrionii and Fonsecaea spp. based on concatenated ITS-BT2 data.
Hamid Badali - One of the best experts on this subject based on the ideXlab platform.
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Genetic differentiation among populations occurring on different continents of Cladophialophora carrionii based on concatenated ITS-BT2 sequence data, Fst indices for Cladophialophora carrionii (n = 67).
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Fst indices interpretion:0–0.05: little genetic differentiation; 0.05–0.15: moderate genetic differentiation0.15–0.25: great genetic differentiation; > 0.25: very great genetic differentiation (isolation)Genetic differentiation among populations occurring on different continents of Cladophialophora carrionii based on concatenated ITS-BT2 sequence data, Fst indices for Cladophialophora carrionii (n = 67).
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Split decomposition network of Cladophialophora carrionii constructed with Splitstree (S1 Text).
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Split decomposition network of Cladophialophora carrionii constructed with Splitstree (S1 Text).
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The population structure of Cladophialophora carrionii and Fonsecaea spp. with Q-hat order.
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Structure 2.3.4 used to establish the population structure of global isolates of C. carrionii isolates and Fonsecaea spp., Cladophialophora carrionii K = 4, admixture model, allele frequencies correlated; Fonsecaea species K = 3, admixture model, allele frequencies correlated. In the Structure display the color between parentheses depict the geographical origin of the haplotype group.
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Neutrality test of Cladophialophora carrionii and Fonsecaea spp. based on concatenated ITS-BT2 data.
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:D* Excess of low-frequency variants, as a result from population expansion, weak negative selection or positive selectionF* Excess of intermediate-frequency alleles, as a result from population bottlenecks, structure and/or balancing selectionNeutrality test of Cladophialophora carrionii and Fonsecaea spp. based on concatenated ITS-BT2 data.
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Cladophialophora abundans a novel species of chaetothyriales isolated from the natural environment
Mycological Progress, 2014Co-Authors: Hamid Badali, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, G. S. De Hoog, Peiying Feng, J B Stielow, Walter A Boeger, Vânia Aparecida VicenteAbstract:A novel species of Cladophialophora is described from the natural environment of the mangrove land crab (Ucides cordatus) along the coast, and from plant sources in Brazil. It is morphologically very similar to Cladophialophora bantiana, though with moderately melanized conidia produced in strongly coherent and poorly branched chains which arise directly from undifferentiated hyphae, but deviates unambiguously on the basis of molecular phylogeny. The species has a maximum growth temperature of 36 °C and seems to be a strict saprobe.
Mohammad Javad Najafzadeh - One of the best experts on this subject based on the ideXlab platform.
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Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis
2016Co-Authors: Mohammad Javad Najafzadeh, Wanqing C Liao, Ruoyu A LiiAbstract:In this study, we developed rapid and sensitive assays for the detection of Cladophialophora carrionii, a common agent of hu-man chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA andMLPA were designed with target sequences in the rDNA internal transcribed spacer gene (ITS) region, and LAMP primers were de-signed using the elongation factor 1 gene (EF1); these probes and primers specifically amplified DNA of isolates of the species. The three techniques were sufficiently specific and sensitive for discriminating target DNA of C. carrionii from that of related Cladophialophora species and other agents of chromoblastomycosis. RCA, MLPA, and LAMP are advantageous in their reliabil-ity and ease of operation compared to standard PCR and conventional methods. Chromoblastomycosis is an endemic, mutilating skin diseasecaused bymelanized fungi. Several species have been reported as etiologic agents, but in the great majority of proven cases, Fon-secaea pedrosoi, Fonsecaea monophora, Fonsecaea nubica, or Cla-dophialophora carrioniiwere concerned.C. carrionii is prevalent i
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Genetic differentiation among populations occurring on different continents of Cladophialophora carrionii based on concatenated ITS-BT2 sequence data, Fst indices for Cladophialophora carrionii (n = 67).
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Fst indices interpretion:0–0.05: little genetic differentiation; 0.05–0.15: moderate genetic differentiation0.15–0.25: great genetic differentiation; > 0.25: very great genetic differentiation (isolation)Genetic differentiation among populations occurring on different continents of Cladophialophora carrionii based on concatenated ITS-BT2 sequence data, Fst indices for Cladophialophora carrionii (n = 67).
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Split decomposition network of Cladophialophora carrionii constructed with Splitstree (S1 Text).
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Split decomposition network of Cladophialophora carrionii constructed with Splitstree (S1 Text).
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The population structure of Cladophialophora carrionii and Fonsecaea spp. with Q-hat order.
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:Structure 2.3.4 used to establish the population structure of global isolates of C. carrionii isolates and Fonsecaea spp., Cladophialophora carrionii K = 4, admixture model, allele frequencies correlated; Fonsecaea species K = 3, admixture model, allele frequencies correlated. In the Structure display the color between parentheses depict the geographical origin of the haplotype group.
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Neutrality test of Cladophialophora carrionii and Fonsecaea spp. based on concatenated ITS-BT2 data.
2015Co-Authors: Shuwen Deng, Hamid Badali, Jacques F. Meis, Clement K. M. Tsui, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Ferry Hagen, Liyue Yang, Jiufeng SunAbstract:D* Excess of low-frequency variants, as a result from population expansion, weak negative selection or positive selectionF* Excess of intermediate-frequency alleles, as a result from population bottlenecks, structure and/or balancing selectionNeutrality test of Cladophialophora carrionii and Fonsecaea spp. based on concatenated ITS-BT2 data.
Wanqing Liao - One of the best experts on this subject based on the ideXlab platform.
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Combination of Amphotericin B and Terbinafine against Melanized Fungi Associated with Chromoblastomycosis
Antimicrobial Agents and Chemotherapy, 2018Co-Authors: Shuwen Deng, G. S. De Hoog, L. Yang, Roxana G. Vitale, Haleh Rafati, M. Seyedmousavi, Ali Tolooe, Wanqing LiaoAbstract:Our in vitro studies showed that a combination of amphotericin B and terbinafine had synergistic effects against the majority of melanized fungi associated with chromoblastomycosis (CBM) and similar infections, including those with Cladophialophora carrionii , Cladophialophora arxii , Exophiala dermatitidis , Exophiala spinifera , Fonsecaea monophora , Fonsecaea nubica , Fonsecaea pedrosoi , and Phialophora verrucosa. This drug combination could provide an option for the treatment of severe or unresponsive cases of CBM, particularly in cases due to species of Fonsecaea and Cladophialophora .
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three isothermal amplification techniques for rapid identification of Cladophialophora carrionii an agent of human chromoblastomycosis
Journal of Clinical Microbiology, 2014Co-Authors: Shuwen Deng, A. Gerrits H. G. Van Den Ende, Mohammad Javad Najafzadeh, Jiufeng Sun, L. Yang, Sybren G De Hoog, Weihua Pan, Min Chen, Wanqing LiaoAbstract:In this study, we developed rapid and sensitive assays for the detection of Cladophialophora carrionii, a common agent of human chromoblastomycosis. The isothermal techniques evaluated were rolling-circle amplification (RCA), multiplex ligation-dependent probe amplification (MLPA), and loop-mediated isothermal amplification (LAMP). The probes for RCA and MLPA were designed with target sequences in the rDNA internal transcribed spacer gene (ITS) region, and LAMP primers were designed using the elongation factor 1α gene (EF1); these probes and primers specifically amplified DNA of isolates of the species. The three techniques were sufficiently specific and sensitive for discriminating target DNA of C. carrionii from that of related Cladophialophora species and other agents of chromoblastomycosis. RCA, MLPA, and LAMP are advantageous in their reliability and ease of operation compared to standard PCR and conventional methods.
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in vitro antifungal susceptibility of Cladophialophora carrionii an agent of human chromoblastomycosis
Antimicrobial Agents and Chemotherapy, 2013Co-Authors: Hamid Badali, Jacques F. Meis, Shuwen Deng, Mohammad Javad Najafzadeh, G. S. De Hoog, L. Yang, B Pan, Ilse Curfsbreuker, Wanqing LiaoAbstract:A global collection of Cladophialophora carrionii strains (n = 81) was tested against nine antifungal drugs. MIC90s of all strains were as follows in increasing order: itraconazole and posaconazole, 0.063 μg/ml; terbinafine, 0.125 μg/ml; isavuconazole and voriconazole, 0.25 μg/ml; caspofungin, 2 μg/ml; micafungin, 4 μg/ml; amphotericin B, 8 μg/ml; and fluconazole, 64 μg/ml.