The Experts below are selected from a list of 3213 Experts worldwide ranked by ideXlab platform
Peng Zhang - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2.
AMB Express, 2018Co-Authors: Li-yun Miao, Lan Zhou, Liu Pan, Fa-jun Song, Wen-wen Jin, Peng ZhangAbstract:The shortage of molecular information for taxol-producing fungi has greatly impeded the understanding of fungal taxol biosynthesis mechanism. In this study, the transcriptome of one taxol-producing endophytic fungus Cladosporium cladosporioides MD2 was sequenced for the first time. About 1.77 Gbp clean reads were generated and further assembled into 16,603 unigenes with an average length of 1110 bp. All of the unigenes were annotated against seven public databases to present the transcriptome characteristics of C. cladosporioides MD2. A total of 12,479 unigenes could be annotated with at least one database, and 1593 unigenes could be annotated in all queried databases. In total, 8425 and 3350 unigenes were categorized into 57 GO functional groups and 262 KEGG pathways, respectively, exhibiting the dominant GO terms and metabolic pathways in the C. cladosporioides MD2 transcriptome. One potential and partial taxol biosynthetic pathway was speculated including 9 unigenes related to terpenoid backbone biosynthesis and 40 unigenes involved in the biosynthetic steps from geranylgeranyl diphosphate to 10-deacetylbaccatin III. These results provided valuable information for the molecular mechanism research of taxol biosynthesis in C. cladosporioides MD2.
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Agrobacterium tumefaciens-mediated transformation of a taxol-producing endophytic fungus, Cladosporium cladosporioides MD2.
Current microbiology, 2011Co-Authors: Peng Zhang, Ting-ting Liu, Pengpeng ZhouAbstract:In this study, an Agrobacteriurn tumefaciens-mediated transformation (ATMT) protocol was successfully developed for the genetic transformation of a taxol-producing fungus, Cladosporium cladosporioides MD2, and the co-cultivation conditions affecting the transformation efficiency were optimized. The optimal transformation conditions were that 1 ml of C. cladosporioides MD2 spore suspension (108 spores/ml) was mixed with an equal volume of A. tumefaciens cultures, which contained 400 μl of A. tumefaciens LBA4404 (OD660 ≈ 0.6) and 600 μl LB medium that were used to make up difference in volume, and the mix cultures were supplemented with 300 μM acetosyringone (AS) and co-cultivated at 26°C and 50 rpm for 48 h. Stable transformants were obtained through analysis of the mitotic stability of inserted T-DNA and the presence of hygromycin resistance gene (hpt II). This study laid a fine groundwork for development of transgenic C. cladosporioides MD2 strains.
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An Endophytic Taxol-Producing Fungus from Taxus media, Cladosporium cladosporioides MD2
Current Microbiology, 2009Co-Authors: Peng Zhang, Pengpeng Zhou, Longjiang YuAbstract:Fermentation processes using taxol-producing fungi other than Taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. In this study, a taxol-producing endophytic fungus strain MD2 was isolated from the inner bark of Taxus media. Strain MD2 produced taxol when grown in potato dextrose liquid medium. The fungal taxol—which was analyzed by ultraviolet, high-performance liquid chromatography and mass spectrometry—was shown to be identical to authentic taxol and 10-deacetylbaccatin III. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal taxol indicated that the fungal taxol produced an NMR spectrum identical to that of authentic taxol. Strain MD2 was identified as Cladosporium cladosporioides according to morphology of the fungal culture, characteristics of the spores, and analysis of 18S rDNA sequence. In addition, 10-deacetylbaccatin III-10-O-acetyl transferase gene of C. cladosporioides MD2 was cloned for the first time and was shown to share 99% identity with that of T. x media and 97% identity with that of T. wallichiana var. mairei.
Diego Romano - One of the best experts on this subject based on the ideXlab platform.
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Sweet-and-salty biocatalysis: Fructooligosaccharides production using Cladosporium cladosporioides in seawater
Process Biochemistry, 2015Co-Authors: Paolo Zambelli, Immacolata Serra, Lucia Fernandez-arrojo, Francisco J. Plou, Lucia Tamborini, Paola Conti, Martina Letizia Contente, Francesco Molinari, Diego RomanoAbstract:Abstract Production of fructooligosaccharides (FOS) from sucrose was obtained using a bioprocess entirely performed in seawater. The halophilic fungus Cladosporium cladosporioides MUT 5506 was grown in a seawater-based medium and mycelium displayed an optimal activity in seawater at 50–60 °C, being stable up to 60 °C. Under optimized conditions in seawater (50 °C, sucrose 600 g/L, lyophilized mycelium 40 g/L), C. cladosporioides gave a maximum FOS yield of 344 g/L after 72 h with a preponderance of 1 F-FOS derivatives (1-kestose 184 g/L, 1-nystose 98 g/L and 1-fructofuranosylnystose 22 g/L), and the noteworthy presence of the non-conventional disaccharide blastose (30 g/L after 144 h). Lyophilized mycelium exhibited good stability in seawater (76% of the initial activity was retained after 15 cycles of reutilization). This proof-of-concept application reports for the first time the production of FOS in a non-conventional medium such as seawater.
Li-yun Miao - One of the best experts on this subject based on the ideXlab platform.
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Transcriptome analysis of a taxol-producing endophytic fungus Cladosporium cladosporioides MD2.
AMB Express, 2018Co-Authors: Li-yun Miao, Lan Zhou, Liu Pan, Fa-jun Song, Wen-wen Jin, Peng ZhangAbstract:The shortage of molecular information for taxol-producing fungi has greatly impeded the understanding of fungal taxol biosynthesis mechanism. In this study, the transcriptome of one taxol-producing endophytic fungus Cladosporium cladosporioides MD2 was sequenced for the first time. About 1.77 Gbp clean reads were generated and further assembled into 16,603 unigenes with an average length of 1110 bp. All of the unigenes were annotated against seven public databases to present the transcriptome characteristics of C. cladosporioides MD2. A total of 12,479 unigenes could be annotated with at least one database, and 1593 unigenes could be annotated in all queried databases. In total, 8425 and 3350 unigenes were categorized into 57 GO functional groups and 262 KEGG pathways, respectively, exhibiting the dominant GO terms and metabolic pathways in the C. cladosporioides MD2 transcriptome. One potential and partial taxol biosynthetic pathway was speculated including 9 unigenes related to terpenoid backbone biosynthesis and 40 unigenes involved in the biosynthetic steps from geranylgeranyl diphosphate to 10-deacetylbaccatin III. These results provided valuable information for the molecular mechanism research of taxol biosynthesis in C. cladosporioides MD2.
Luis Antonio Suita De Castro - One of the best experts on this subject based on the ideXlab platform.
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ecthyma like phaeohyphomycosis caused by Cladosporium cladosporioides
Mycoses, 2010Co-Authors: Rodrigo Pereira Duquia, Hiram Larangeira De Almeida, Gerson Vettorato, Nara Moreira Rocha, Luis Antonio Suita De CastroAbstract:A case of cutaneous phaeohyphomycosis caused by Cladosporium cladosporioides in a 50-year-old housewife is described. The clinical presentation was an ecthyma-like crusted lesion on the back of her left hand. Scanning electron microscopy of the culture showed the conidiophores and the limoniform or ellipsoidal conidia, with a slightly verrucous surface. The lesion was removed surgically, with no relapses after 6-month follow up.
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Ecthyma‐like phaeohyphomycosis caused by Cladosporium cladosporioides
Mycoses, 2009Co-Authors: Rodrigo Pereira Duquia, Hiram Larangeira De Almeida, Gerson Vettorato, Nara Moreira Rocha, Luis Antonio Suita De CastroAbstract:A case of cutaneous phaeohyphomycosis caused by Cladosporium cladosporioides in a 50-year-old housewife is described. The clinical presentation was an ecthyma-like crusted lesion on the back of her left hand. Scanning electron microscopy of the culture showed the conidiophores and the limoniform or ellipsoidal conidia, with a slightly verrucous surface. The lesion was removed surgically, with no relapses after 6-month follow up.
Dehai Li - One of the best experts on this subject based on the ideXlab platform.
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clindanones a and b and cladosporols f and g polyketides from the deep sea derived fungus Cladosporium cladosporioides hdn14 342
RSC Advances, 2016Co-Authors: Zhenzhen Zhang, Xueqian He, Qianqun Gu, Dehai LiAbstract:Four new tetralone derivatives, clindanones A and B (1 and 2) and cladosporols F and G (3 and 4), together with three known biogenetically related polyketides (5–7), were isolated from the deep-sea derived fungus Cladosporium cladosporioides HDN14-342. The structures of 1–4, including absolute configurations, were deduced based on MS, NMR and TD-DFT calculations of specific ECD spectra. The absolute configurations of the known cladosporols C (5) and E (6) were also revised. Compounds 1 and 2 possessed new dimeric forms of the skeleton composed by coupling of indanone and 1-tetralone units, and 4 showed the best cytotoxic activity against HeLa cells with an IC50 value of 3.9 μM.