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Carey-ann D. Burnham - One of the best experts on this subject based on the ideXlab platform.

  • Clinical Microbiology is growing up the total laboratory automation revolution
    Clinical Chemistry, 2019
    Co-Authors: Adam L Bailey, Nathan A. Ledeboer, Carey-ann D. Burnham
    Abstract:

    Background: Historically, culture-based Microbiology laboratory testing has relied on manual methods, and automated methods (such as those that have revolutionized Clinical chemistry and hematology over the past several decades) were largely absent from the Clinical Microbiology laboratory. However, an increased demand for Microbiology testing and standardization of sample-collection devices for Microbiology culture, as well as a dwindling supply of Microbiology technologists, has driven the adoption of automated methods for culture-based laboratory testing in Clinical Microbiology. Content: We describe systems currently enabling total laboratory automation (TLA) for culture-based Microbiology testing. We describe the general components of a Microbiology automation system and the various functions of these instruments. We then introduce the 2 most widely used systems currently on the market: Becton Dickinson’s Kiestra TLA and Copan’s WASPLab. We discuss the impact of TLA on metrics such as turnaround time and recovery of microorganisms, providing a review of the current literature and perspectives from laboratory directors, managers, and technical staff. Finally, we provide an outlook for future advances in TLA for Microbiology with a focus on artificial intelligence for automated culture interpretation. Summary: TLA is playing an increasingly important role in Clinical Microbiology. Although challenges remain, TLA has great potential to affect laboratory efficiency, turnaround time, and the overall quality of culture-based Microbiology testing.

  • the brief case a new feature in journal of Clinical Microbiology
    Journal of Clinical Microbiology, 2016
    Co-Authors: Carey-ann D. Burnham, Andrew B Onderdonk, Alexander J. Mcadam
    Abstract:

    The views expressed in this Editorial do not necessarily reflect the views of the journal or of ASM. With this issue of Journal of Clinical Microbiology (JCM), we are very pleased to introduce a new feature, The Brief Case. (Thanks to our friend Dr. Michael Dunne for suggesting the name.) The

  • Automation in the Clinical Microbiology Laboratory
    Clinical chemistry, 2013
    Co-Authors: Carey-ann D. Burnham, Gilbert Greub, Susan M. Novak, W. Michael Dunne, Robin Patel
    Abstract:

    The Clinical Microbiology laboratory has historically been considered “low-tech,” especially when compared to the Clinical chemistry laboratory. However, systems are emerging for the Clinical Microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of growth on culture media, identification of microorganisms, susceptibility testing, and extraction and detection of nucleic acids in Clinical samples. As a result, the workflow in the Microbiology laboratory is changing at a rapid pace and microbiologists have the challenge of selecting the most appropriate, Clinically useful, and cost-effective automation for their laboratories. We have asked 4 experts in this field, from Clinical Microbiology laboratories in the US and Europe, as well as from industry, to comment on the feasibility and impact of automation in the Clinical Microbiology laboratory. Are you currently using or do you anticipate using an automation platform in your Microbiology laboratory? If yes, which sections of your laboratory are automated? Robin Patel: Mayo Clinic's Clinical Microbiology laboratory has been performing testing since 1911. Although select tests today resemble those performed a century ago, we have many examples of automated, state-of-the-art tests. These include blood cultures, infectious disease serologic platforms, and nucleic acid and proteomic diagnostics, to name a few. For over 2 decades, Microbiology laboratories have been using automated blood culture instruments that “sense” microbial growth in blood culture bottles and “flag” positive bottles for immediate attention by laboratory technologists. Before the availability of such systems (in the not-so-distant past), laboratory technologists manually evaluated each blood culture bottle on multiple occasions. Technologists today could not fathom returning to the manual approach used a mere 3 decades ago. As with many chemistry tests, a myriad of infectious disease serologic tests are performed on automated platforms. Nucleic acid diagnostics, which have been used in our …

  • What Happened to Research in Clinical Microbiology in the United States
    Journal of Clinical Microbiology, 2011
    Co-Authors: Paul P. Bourbeau, Carey-ann D. Burnham
    Abstract:

    The title of this article is loaded with negative innuendo—the question is not “What is the state of research in Clinical Microbiology in the United States” but, rather, “What has happened to happened to research in Clinical Microbiology in the United States.” We begin with the premise

W. Michael Dunne - One of the best experts on this subject based on the ideXlab platform.

  • Automation in the Clinical Microbiology Laboratory
    Clinical chemistry, 2013
    Co-Authors: Carey-ann D. Burnham, Gilbert Greub, Susan M. Novak, W. Michael Dunne, Robin Patel
    Abstract:

    The Clinical Microbiology laboratory has historically been considered “low-tech,” especially when compared to the Clinical chemistry laboratory. However, systems are emerging for the Clinical Microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of growth on culture media, identification of microorganisms, susceptibility testing, and extraction and detection of nucleic acids in Clinical samples. As a result, the workflow in the Microbiology laboratory is changing at a rapid pace and microbiologists have the challenge of selecting the most appropriate, Clinically useful, and cost-effective automation for their laboratories. We have asked 4 experts in this field, from Clinical Microbiology laboratories in the US and Europe, as well as from industry, to comment on the feasibility and impact of automation in the Clinical Microbiology laboratory. Are you currently using or do you anticipate using an automation platform in your Microbiology laboratory? If yes, which sections of your laboratory are automated? Robin Patel: Mayo Clinic's Clinical Microbiology laboratory has been performing testing since 1911. Although select tests today resemble those performed a century ago, we have many examples of automated, state-of-the-art tests. These include blood cultures, infectious disease serologic platforms, and nucleic acid and proteomic diagnostics, to name a few. For over 2 decades, Microbiology laboratories have been using automated blood culture instruments that “sense” microbial growth in blood culture bottles and “flag” positive bottles for immediate attention by laboratory technologists. Before the availability of such systems (in the not-so-distant past), laboratory technologists manually evaluated each blood culture bottle on multiple occasions. Technologists today could not fathom returning to the manual approach used a mere 3 decades ago. As with many chemistry tests, a myriad of infectious disease serologic tests are performed on automated platforms. Nucleic acid diagnostics, which have been used in our …

  • New Technologies in Clinical Microbiology
    Journal of Clinical Microbiology, 2011
    Co-Authors: Donna M. Wolk, W. Michael Dunne
    Abstract:

    Rapid identification of microorganisms in the Clinical Microbiology laboratory can be of great value for selection of optimal patient management strategies for infections caused by bacteria, viruses, fungi, mycobacteria, and parasites. Rapid identification of microorganisms in Clinical samples enables expedient de-escalation from broad-spectrum agents to targeted antimicrobial therapy. The switch to tailored therapy minimizes risks of antibiotics, namely, disruption of normal flora, toxic side effects, and selective pressure. There is a critical need for new technologies in Clinical Microbiology, particularly for bloodstream infections, in which associated mortality is among the highest of all infections. Just as importantly, there is a need for the Clinical laboratory community to embrace the practices of evidence-based interventional laboratory medicine and collaborate in translational research projects to establish the Clinical utility, cost benefit, and impact of new technologies.

  • The Business of Clinical Microbiology
    Journal of Clinical Microbiology, 2011
    Co-Authors: W. Michael Dunne
    Abstract:

    The final session of Camp Clin Micro was perhaps unique in that it dealt with subjects that are rarely if ever discussed in other Clinical Microbiology venues. The first discussion was entitled “Enhancing the Laboratory/Industry Interface” and was led by David Durack. The evolution of diagnostic

  • Clinical Microbiology in the Year 2015
    Journal of Clinical Microbiology, 2011
    Co-Authors: W. Michael Dunne
    Abstract:

    This session was subdivided into four discussion sections, each with the charge of looking into the crystal ball and speculating on the future of Clinical Microbiology from a number of unique perspectives. Five years or less is not a long period of time for prognostication, but the organizers felt

  • Postmortem Analysis and the Role of the Clinical Microbiology Laboratory
    Academic Forensic Pathology, 2011
    Co-Authors: Robin R. Craven, Lanette R. Hamilton, Carl O. Deetz, W. Michael Dunne
    Abstract:

    The Clinical Microbiology laboratory frequently receives specimens for culture collected at autopsy. The results generated from these culture requests can either provide valuable information or pro...

Nathan A. Ledeboer - One of the best experts on this subject based on the ideXlab platform.

  • Clinical Microbiology is growing up the total laboratory automation revolution
    Clinical Chemistry, 2019
    Co-Authors: Adam L Bailey, Nathan A. Ledeboer, Carey-ann D. Burnham
    Abstract:

    Background: Historically, culture-based Microbiology laboratory testing has relied on manual methods, and automated methods (such as those that have revolutionized Clinical chemistry and hematology over the past several decades) were largely absent from the Clinical Microbiology laboratory. However, an increased demand for Microbiology testing and standardization of sample-collection devices for Microbiology culture, as well as a dwindling supply of Microbiology technologists, has driven the adoption of automated methods for culture-based laboratory testing in Clinical Microbiology. Content: We describe systems currently enabling total laboratory automation (TLA) for culture-based Microbiology testing. We describe the general components of a Microbiology automation system and the various functions of these instruments. We then introduce the 2 most widely used systems currently on the market: Becton Dickinson’s Kiestra TLA and Copan’s WASPLab. We discuss the impact of TLA on metrics such as turnaround time and recovery of microorganisms, providing a review of the current literature and perspectives from laboratory directors, managers, and technical staff. Finally, we provide an outlook for future advances in TLA for Microbiology with a focus on artificial intelligence for automated culture interpretation. Summary: TLA is playing an increasingly important role in Clinical Microbiology. Although challenges remain, TLA has great potential to affect laboratory efficiency, turnaround time, and the overall quality of culture-based Microbiology testing.

  • emerging technologies for the Clinical Microbiology laboratory
    Clinical Microbiology Reviews, 2014
    Co-Authors: Blake W Buchan, Nathan A. Ledeboer
    Abstract:

    In this review we examine the literature related to emerging technologies that will help to reshape the Clinical Microbiology laboratory. These topics include nucleic acid amplification tests such as isothermal and point-of-care molecular diagnostics, multiplexed panels for syndromic diagnosis, digital PCR, next-generation sequencing, and automation of molecular tests. We also review matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) and electrospray ionization (ESI) mass spectrometry methods and their role in identification of microorganisms. Lastly, we review the shift to liquid-based Microbiology and the integration of partial and full laboratory automation that are beginning to impact the Clinical Microbiology laboratory.

  • Automation in Clinical Microbiology
    Journal of Clinical Microbiology, 2013
    Co-Authors: Paul P. Bourbeau, Nathan A. Ledeboer
    Abstract:

    Editor's Note: In this issue of the Journal of Clinical Microbiology, Paul Bourbeau and Nate Ledeboer provide an informed review of an exciting new concept in Clinical Microbiology, the use of instrumentation to automate the front-end processing and workup of specimens submitted to a laboratory for analysis. The potential value of such instrumentation includes the possibility of substantial cost savings, standardization of initial specimen processing, more rapid and consistent provision of both identification and antimicrobial susceptibility test results, and a diminished risk for laboratory-acquired infections. However, as with any new diagnostic modality in Clinical Microbiology, there now exists a pressing need for investigations aimed at elucidating the performance characteristics of this new technology. Going forward, it will be imperative that laboratorians assess this new technology in objective, comparative, and preferably prospective Clinical studies. Such studies will be necessary to define the true, rather than perceived or hoped-for, value of front-end and total laboratory automation in Clinical Microbiology. The Journal of Clinical Microbiology enthusiastically awaits submission of manuscripts that report the results of such investigations. Gary V. Doern, Editor in Chief, Journal of Clinical Microbiology

Robin Patel - One of the best experts on this subject based on the ideXlab platform.

  • Automation in the Clinical Microbiology Laboratory
    Clinical chemistry, 2013
    Co-Authors: Carey-ann D. Burnham, Gilbert Greub, Susan M. Novak, W. Michael Dunne, Robin Patel
    Abstract:

    The Clinical Microbiology laboratory has historically been considered “low-tech,” especially when compared to the Clinical chemistry laboratory. However, systems are emerging for the Clinical Microbiology laboratory with the potential to automate almost all areas of testing, including inoculation of primary culture plates, detection of growth on culture media, identification of microorganisms, susceptibility testing, and extraction and detection of nucleic acids in Clinical samples. As a result, the workflow in the Microbiology laboratory is changing at a rapid pace and microbiologists have the challenge of selecting the most appropriate, Clinically useful, and cost-effective automation for their laboratories. We have asked 4 experts in this field, from Clinical Microbiology laboratories in the US and Europe, as well as from industry, to comment on the feasibility and impact of automation in the Clinical Microbiology laboratory. Are you currently using or do you anticipate using an automation platform in your Microbiology laboratory? If yes, which sections of your laboratory are automated? Robin Patel: Mayo Clinic's Clinical Microbiology laboratory has been performing testing since 1911. Although select tests today resemble those performed a century ago, we have many examples of automated, state-of-the-art tests. These include blood cultures, infectious disease serologic platforms, and nucleic acid and proteomic diagnostics, to name a few. For over 2 decades, Microbiology laboratories have been using automated blood culture instruments that “sense” microbial growth in blood culture bottles and “flag” positive bottles for immediate attention by laboratory technologists. Before the availability of such systems (in the not-so-distant past), laboratory technologists manually evaluated each blood culture bottle on multiple occasions. Technologists today could not fathom returning to the manual approach used a mere 3 decades ago. As with many chemistry tests, a myriad of infectious disease serologic tests are performed on automated platforms. Nucleic acid diagnostics, which have been used in our …

  • MALDI-TOF Mass Spectrometry: Transformative Proteomics for Clinical Microbiology
    Clinical chemistry, 2013
    Co-Authors: Robin Patel
    Abstract:

    Clinical Microbiology laboratories today operate with the use of a blend of high- and low-technology procedures. Despite huge steps forward in diagnostic methods based on nucleic acid amplification, Clinical Microbiology laboratories still rely heavily on culturing bacteria and fungi for diagnostic purposes because of the plethora and diversity of potentially isolated organisms. This process is typically done on solid (e.g., blood agar) and occasionally in liquid (i.e., broth) media. Once plate growth is observed (the so-called “colony”), the challenge is accurate identification of that growth. Historically, this identification has been considered to be a complex task, and numerous textbooks have been written about it. Traditional (phenotypic) identification of bacteria and fungi involves examination of morphologic characteristics, staining, and conducting biochemical tests (either manually or as an automated panel). Manual biochemical assays (e.g., catalase, oxidase) may provide a rapid turnaround time, but they identify only a limited number of organism types. Automated systems (e.g., VITEK®, bioMerieux; BD Phoenix™ Automated Microbiology System; MicroScan®, Siemens Healthcare Diagnostics) identify a broader range of organisms, but suffer from longer turnaround time and expensive consumables. Furthermore, the user often must have a priori knowledge of the organism type (e.g., gram-negative bacillus) being tested. Introduction of MALDI-TOF mass spectrometry into the Clinical Microbiology laboratory has markedly altered workflow, allowing bacterial and fungal colonies to be accurately, rapidly, and inexpensively identified. Contemporary applications in Clinical Microbiology combine the powerful capabilities of mass spectrometry and bioinformatics to yield a novel methodology for bacterial and fungal identification. Utilization of MALDI-TOF mass spectrometry in the Clinical Microbiology laboratory is explained in its simplest form by discussing the routine workflow of a Microbiology technologist. Starting from a single isolated colony on a culture plate, a colony is “picked” to a spot on a MALDI-TOF mass spectrometry plate, which is a solid, typically …

Christopher D. Doern - One of the best experts on this subject based on the ideXlab platform.

  • Pocket Guide to Clinical Microbiology, Fourth Edition
    2018
    Co-Authors: Christopher D. Doern
    Abstract:

    Pocket Guide to Clinical Microbiology is now available on Wiley.comMembers, use the code ASM20 at check out to receive your 20% discount. Quick reference to Clinical Microbiology If you work in the Clinical laboratory, this pocket guide will help you confidently identify most organisms you could encounter. This useful updated edition continues to present valuable quick-reference information to the Clinical Microbiology community in a small package. Along with specifics on pathogenic microorganisms, there is updated information on effectively using essential molecular diagnostic techniques for today’s challenges. You will find guidance on: MALDI-TOF MS performance for individual bacteria, mycobacteria, and fungi Nucleic acid amplification testing/PCR and help interpreting genetic sequencing results Susceptibility testing, with methods and interpretive criteria for most organism/antibiotic combinations Antimicrobial resistance mechanisms and resistance profiles for common organisms Paperback, 420 pages, index.

  • #N# Manual of Clinical Microbiology - Automation and Design of the Clinical Microbiology Laboratory
    Manual of Clinical Microbiology, 2015
    Co-Authors: Christopher D. Doern, Martin Holfelder
    Abstract:

    This chapter discusses laboratory design and the growing impact of laboratory automation. The chapter is divided into two sections. The first section addresses more traditional considerations of laboratory design such as staffing, workflow, laboratory location, and the role of efficiency programs such as lean and Six Sigma. The second section discusses currently available systems for automating the Clinical Microbiology laboratory. Individual technologies for specimen processing, organism identification, and susceptibility testing as well as total lab automation are covered. Important considerations for purchase and utilization are outlined to aid readers in deciding which technology is right for their lab and how best to approach implementation.