The Experts below are selected from a list of 2985 Experts worldwide ranked by ideXlab platform
An-ping Zeng - One of the best experts on this subject based on the ideXlab platform.
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Improved electrocompetence and metabolic engineering of Clostridium pasteurianum reveals a new regulation pattern of glycerol fermentation.
Engineering in life sciences, 2018Co-Authors: Rebekka Schmitz, Wael Sabra, Philipp Arbter, Yaeseong Hong, Tyll Utesch, An-ping ZengAbstract:Clostridium pasteurianum produces industrially valuable chemicals such as n-butanol and 1,3-propanediol from fermentations of glycerol and glucose. Metabolic engineering for increased yields of selective compounds is not well established in this microorganism. In order to study carbon fluxes and to selectively increase butanol yields, we integrated the latest advances in genome editing to obtain an electrocompetent Clostridium pasteurianum strain for further engineering. Deletion of the glycerol dehydratase large subunit (dhaB) using an adapted S. pyogenes Type II CRISPR/Cas9 nickase system resulted in a 1,3-propanediol-deficient mutant producing butanol as the main product. Surprisingly, the mutant was able to grow on glycerol as the sole carbon source. In spite of reduced growth, butanol yields were highly increased. Metabolic flux analysis revealed an important role of the newly identified electron bifurcation pathway for crotonyl-CoA to butyryl-CoA conversion in the regulation of redox balance. Compared to the parental strain, the electron bifurcation pathway flux of the dhaB mutant increased from 8 to 46% of the overall flux from crotonyl-CoA to butyryl-CoA and butanol, indicating a new, 1,3-propanediol-independent pattern of glycerol fermentation in Clostridium pasteurianum.
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Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
Microbial cell factories, 2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Background Clostridium pasteurianum is becoming increasingly attractive for the production of chemicals and fuels such as n-butanol and 1,3-propanediol. Previously we have shown that dual substrate fermentation using glucose and glycerol enhanced the cell growth and butanol production significantly. Although C. pasteurianum can grow efficiently with either glucose or glycerol alone, under certain conditions, glucose limitation in the mixed substrate fermentation leads to growth cessation. To understand this phenomenon and for process optimization, fermentation experiments were performed in the presence of excess glycerol but with varied initial concentrations of glucose which were followed by physiological, metabolic and proteomic analyses.
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MOESM1 of Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Additional file 1: Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
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Draft Genome Sequence of Type Strain Clostridium pasteurianum DSM 525 (ATCC 6013), a Promising Producer of Chemicals and Fuels.
Genome announcements, 2013Co-Authors: Sugima Rappert, Wael Sabra, Wei Wang, Lifu Song, An-ping ZengAbstract:Clostridium pasteurianum, an anaerobic bacterium able to utilize atmospheric free nitrogen for biosynthesis, has recently been proven to be a promising producer of chemicals and fuels, such as 1,3-propanediol and n-butanol. Here, we report the high-quality draft genome sequence of DSM 525, a type strain of C. pasteurianum.
Wael Sabra - One of the best experts on this subject based on the ideXlab platform.
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Improved electrocompetence and metabolic engineering of Clostridium pasteurianum reveals a new regulation pattern of glycerol fermentation.
Engineering in life sciences, 2018Co-Authors: Rebekka Schmitz, Wael Sabra, Philipp Arbter, Yaeseong Hong, Tyll Utesch, An-ping ZengAbstract:Clostridium pasteurianum produces industrially valuable chemicals such as n-butanol and 1,3-propanediol from fermentations of glycerol and glucose. Metabolic engineering for increased yields of selective compounds is not well established in this microorganism. In order to study carbon fluxes and to selectively increase butanol yields, we integrated the latest advances in genome editing to obtain an electrocompetent Clostridium pasteurianum strain for further engineering. Deletion of the glycerol dehydratase large subunit (dhaB) using an adapted S. pyogenes Type II CRISPR/Cas9 nickase system resulted in a 1,3-propanediol-deficient mutant producing butanol as the main product. Surprisingly, the mutant was able to grow on glycerol as the sole carbon source. In spite of reduced growth, butanol yields were highly increased. Metabolic flux analysis revealed an important role of the newly identified electron bifurcation pathway for crotonyl-CoA to butyryl-CoA conversion in the regulation of redox balance. Compared to the parental strain, the electron bifurcation pathway flux of the dhaB mutant increased from 8 to 46% of the overall flux from crotonyl-CoA to butyryl-CoA and butanol, indicating a new, 1,3-propanediol-independent pattern of glycerol fermentation in Clostridium pasteurianum.
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Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
Microbial cell factories, 2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Background Clostridium pasteurianum is becoming increasingly attractive for the production of chemicals and fuels such as n-butanol and 1,3-propanediol. Previously we have shown that dual substrate fermentation using glucose and glycerol enhanced the cell growth and butanol production significantly. Although C. pasteurianum can grow efficiently with either glucose or glycerol alone, under certain conditions, glucose limitation in the mixed substrate fermentation leads to growth cessation. To understand this phenomenon and for process optimization, fermentation experiments were performed in the presence of excess glycerol but with varied initial concentrations of glucose which were followed by physiological, metabolic and proteomic analyses.
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MOESM1 of Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Additional file 1: Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
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Draft Genome Sequence of Type Strain Clostridium pasteurianum DSM 525 (ATCC 6013), a Promising Producer of Chemicals and Fuels.
Genome announcements, 2013Co-Authors: Sugima Rappert, Wael Sabra, Wei Wang, Lifu Song, An-ping ZengAbstract:Clostridium pasteurianum, an anaerobic bacterium able to utilize atmospheric free nitrogen for biosynthesis, has recently been proven to be a promising producer of chemicals and fuels, such as 1,3-propanediol and n-butanol. Here, we report the high-quality draft genome sequence of DSM 525, a type strain of C. pasteurianum.
Jacques Meyer - One of the best experts on this subject based on the ideXlab platform.
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Cloning and sequencing of the gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum.
Biochimica et biophysica acta, 1993Co-Authors: Jacques MeyerAbstract:A 1348 basepair EcoRI fragment of genomic DNA containing the [2Fe-2S] ferredoxin gene from Clostridium pasteurianum has been cloned and sequenced. The translated protein sequence is identical to the sequence of the protein as previously determined by Edman chemistry. Two potential open reading frames occur upstream and downstream of the ferredoxin gene. However, the features surrounding the latter gene strongly suggest that it constitutes a separate transcriptional unit. The sequence data provide no evidence that this ferredoxin is involved in nitrogen fixation.
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Cloning and expression in Escherichia coli of the gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum.
Biochemical and biophysical research communications, 1993Co-Authors: J. Fujinaga, Jacques MeyerAbstract:The gene encoding the [2Fe-2S] ferredoxin from Clostridium pasteurianum has been amplified from genomic DNA by the polymerase chain reaction and cloned under the control of a promoter specifically recognized by T7 RNA polymerase. The protein has been overproduced in E. coli and found to be identical to its native counterpart purified from C. pasteurianum, including the molecular weight, the N-terminal sequence and the spectroscopic properties of the [2Fe-2S] chromophore.
Wei Wang - One of the best experts on this subject based on the ideXlab platform.
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Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
Microbial cell factories, 2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Background Clostridium pasteurianum is becoming increasingly attractive for the production of chemicals and fuels such as n-butanol and 1,3-propanediol. Previously we have shown that dual substrate fermentation using glucose and glycerol enhanced the cell growth and butanol production significantly. Although C. pasteurianum can grow efficiently with either glucose or glycerol alone, under certain conditions, glucose limitation in the mixed substrate fermentation leads to growth cessation. To understand this phenomenon and for process optimization, fermentation experiments were performed in the presence of excess glycerol but with varied initial concentrations of glucose which were followed by physiological, metabolic and proteomic analyses.
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MOESM1 of Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Additional file 1: Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
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Draft Genome Sequence of Type Strain Clostridium pasteurianum DSM 525 (ATCC 6013), a Promising Producer of Chemicals and Fuels.
Genome announcements, 2013Co-Authors: Sugima Rappert, Wael Sabra, Wei Wang, Lifu Song, An-ping ZengAbstract:Clostridium pasteurianum, an anaerobic bacterium able to utilize atmospheric free nitrogen for biosynthesis, has recently been proven to be a promising producer of chemicals and fuels, such as 1,3-propanediol and n-butanol. Here, we report the high-quality draft genome sequence of DSM 525, a type strain of C. pasteurianum.
Sruthi Surandram - One of the best experts on this subject based on the ideXlab platform.
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Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
Microbial cell factories, 2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Background Clostridium pasteurianum is becoming increasingly attractive for the production of chemicals and fuels such as n-butanol and 1,3-propanediol. Previously we have shown that dual substrate fermentation using glucose and glycerol enhanced the cell growth and butanol production significantly. Although C. pasteurianum can grow efficiently with either glucose or glycerol alone, under certain conditions, glucose limitation in the mixed substrate fermentation leads to growth cessation. To understand this phenomenon and for process optimization, fermentation experiments were performed in the presence of excess glycerol but with varied initial concentrations of glucose which were followed by physiological, metabolic and proteomic analyses.
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MOESM1 of Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations
2016Co-Authors: Wael Sabra, Wei Wang, Sruthi Surandram, Christin Groeger, An-ping ZengAbstract:Additional file 1: Fermentation of mixed substrates by Clostridium pasteurianum and its physiological, metabolic and proteomic characterizations