The Experts below are selected from a list of 978 Experts worldwide ranked by ideXlab platform
Stanislaw Moskalewski - One of the best experts on this subject based on the ideXlab platform.
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology and Biotechnology, 2011Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains ( r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain ( r = 0.62) and collagenase ( r = 0.87) production, and this effect was statistically significant ( P
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology & Biotechnology, 2010Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains (r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain (r = 0.62) and collagenase (r = 0.87) production, and this effect was statistically significant (P < 0.05). Negative non-significant correlations were detected: (a) in lethal activities of studied strains and Clostripain, or collagenase activities, (b) in vacuolating activities and protease production.
Gayane Martirosian - One of the best experts on this subject based on the ideXlab platform.
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology and Biotechnology, 2011Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains ( r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain ( r = 0.62) and collagenase ( r = 0.87) production, and this effect was statistically significant ( P
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology & Biotechnology, 2010Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains (r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain (r = 0.62) and collagenase (r = 0.87) production, and this effect was statistically significant (P < 0.05). Negative non-significant correlations were detected: (a) in lethal activities of studied strains and Clostripain, or collagenase activities, (b) in vacuolating activities and protease production.
-
Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
Enzyme and Microbial Technology, 2006Co-Authors: Jarosław Jóźwiak, Aldona Komar, Ewa Jankowska, Gayane MartirosianAbstract:Abstract Clostridium histolyticum supernatant, thanks to intrinsic proteases, exerts cytotoxic effect on cells and tissues. In our study we compared the ability of five different protease inhibitors to block cytotoxic activity of C. histolyticum supertanant: aprotinin, leupeptin, phenylmethylsulphonyl fluoride (PMSF), l -1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin. Inhibitors of serine proteases nearly completely blocked cytotoxic effect, especially in lower supernatant concentrations. At the same time TLCK, known to irreversibly block Clostripain obtained from C. histolyticum , blocked enzymatic activity of Clostripain, but not cytotoxic effect of the supernatant. We conclude that the remaining cytotoxicity must be the effect of the activity of another factor that is contained in the supernatant and that can be isolated and tested in the presence of TLCK.
-
Determination of the cytotoxic effect of Clostridium histolyticum culture supernatant on HeLa cells in the presence of protease inhibitors
Fems Immunology and Medical Microbiology, 2005Co-Authors: Jarosław Jóźwiak, Aldona Komar, Ewa Jankowska, Gayane MartirosianAbstract:Clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. This includes lethal toxin, Clostripain and high-potassium-sensitive toxin. Since the number of C. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of C. histolyticum. In this study we evaluated in vitro the influence of four common protease inhibitors: aprotinin, phenylmethylsulphonyl fluoride (PMSF), l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin on the toxicity of C. histolyticum supernatant towards human epithelial HeLa cells. We show that aprotinin has no effect, while PMSF, TLCK and chymostatin potentiate the cytotoxic activity of C. histolyticum, probably by hindering natural defence mechanisms of cells. In addition, PMSF and TLCK block Clostripain enzymatic activity, while chymostatin leaves it intact. Elevated cytotoxicity of the supernatant is not related to the quantity of high-potassium-sensitive toxin, as was reported previously, since desalted supernatant still exerted its strong toxic effect. Our results show that addition of protease inhibitors for treating diseases complicated by concurrent C. histolyticum infection must require special attention.
Jarosław Jóźwiak - One of the best experts on this subject based on the ideXlab platform.
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology and Biotechnology, 2011Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains ( r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain ( r = 0.62) and collagenase ( r = 0.87) production, and this effect was statistically significant ( P
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology & Biotechnology, 2010Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains (r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain (r = 0.62) and collagenase (r = 0.87) production, and this effect was statistically significant (P < 0.05). Negative non-significant correlations were detected: (a) in lethal activities of studied strains and Clostripain, or collagenase activities, (b) in vacuolating activities and protease production.
-
Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
Enzyme and Microbial Technology, 2006Co-Authors: Jarosław Jóźwiak, Aldona Komar, Ewa Jankowska, Gayane MartirosianAbstract:Abstract Clostridium histolyticum supernatant, thanks to intrinsic proteases, exerts cytotoxic effect on cells and tissues. In our study we compared the ability of five different protease inhibitors to block cytotoxic activity of C. histolyticum supertanant: aprotinin, leupeptin, phenylmethylsulphonyl fluoride (PMSF), l -1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin. Inhibitors of serine proteases nearly completely blocked cytotoxic effect, especially in lower supernatant concentrations. At the same time TLCK, known to irreversibly block Clostripain obtained from C. histolyticum , blocked enzymatic activity of Clostripain, but not cytotoxic effect of the supernatant. We conclude that the remaining cytotoxicity must be the effect of the activity of another factor that is contained in the supernatant and that can be isolated and tested in the presence of TLCK.
-
Determination of the cytotoxic effect of Clostridium histolyticum culture supernatant on HeLa cells in the presence of protease inhibitors
Fems Immunology and Medical Microbiology, 2005Co-Authors: Jarosław Jóźwiak, Aldona Komar, Ewa Jankowska, Gayane MartirosianAbstract:Clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. This includes lethal toxin, Clostripain and high-potassium-sensitive toxin. Since the number of C. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of C. histolyticum. In this study we evaluated in vitro the influence of four common protease inhibitors: aprotinin, phenylmethylsulphonyl fluoride (PMSF), l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin on the toxicity of C. histolyticum supernatant towards human epithelial HeLa cells. We show that aprotinin has no effect, while PMSF, TLCK and chymostatin potentiate the cytotoxic activity of C. histolyticum, probably by hindering natural defence mechanisms of cells. In addition, PMSF and TLCK block Clostripain enzymatic activity, while chymostatin leaves it intact. Elevated cytotoxicity of the supernatant is not related to the quantity of high-potassium-sensitive toxin, as was reported previously, since desalted supernatant still exerted its strong toxic effect. Our results show that addition of protease inhibitors for treating diseases complicated by concurrent C. histolyticum infection must require special attention.
Aldona Komar - One of the best experts on this subject based on the ideXlab platform.
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology and Biotechnology, 2011Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains ( r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain ( r = 0.62) and collagenase ( r = 0.87) production, and this effect was statistically significant ( P
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology & Biotechnology, 2010Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains (r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain (r = 0.62) and collagenase (r = 0.87) production, and this effect was statistically significant (P < 0.05). Negative non-significant correlations were detected: (a) in lethal activities of studied strains and Clostripain, or collagenase activities, (b) in vacuolating activities and protease production.
-
Inhibition of Clostridium histolyticum supernatant cytotoxic activity by protease inhibitors
Enzyme and Microbial Technology, 2006Co-Authors: Jarosław Jóźwiak, Aldona Komar, Ewa Jankowska, Gayane MartirosianAbstract:Abstract Clostridium histolyticum supernatant, thanks to intrinsic proteases, exerts cytotoxic effect on cells and tissues. In our study we compared the ability of five different protease inhibitors to block cytotoxic activity of C. histolyticum supertanant: aprotinin, leupeptin, phenylmethylsulphonyl fluoride (PMSF), l -1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin. Inhibitors of serine proteases nearly completely blocked cytotoxic effect, especially in lower supernatant concentrations. At the same time TLCK, known to irreversibly block Clostripain obtained from C. histolyticum , blocked enzymatic activity of Clostripain, but not cytotoxic effect of the supernatant. We conclude that the remaining cytotoxicity must be the effect of the activity of another factor that is contained in the supernatant and that can be isolated and tested in the presence of TLCK.
-
Determination of the cytotoxic effect of Clostridium histolyticum culture supernatant on HeLa cells in the presence of protease inhibitors
Fems Immunology and Medical Microbiology, 2005Co-Authors: Jarosław Jóźwiak, Aldona Komar, Ewa Jankowska, Gayane MartirosianAbstract:Clostridium histolyticum culture supernatant contains numerous enzymes, which exert a cytotoxic effect on host cells. This includes lethal toxin, Clostripain and high-potassium-sensitive toxin. Since the number of C. histolyticum infections increased during the last several years, it seems worthwhile to evaluate whether protease inhibitors, used for the treatment of many diseases, could influence toxicity, and thus, pathogenicity of C. histolyticum. In this study we evaluated in vitro the influence of four common protease inhibitors: aprotinin, phenylmethylsulphonyl fluoride (PMSF), l-1-chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl (TLCK) and chymostatin on the toxicity of C. histolyticum supernatant towards human epithelial HeLa cells. We show that aprotinin has no effect, while PMSF, TLCK and chymostatin potentiate the cytotoxic activity of C. histolyticum, probably by hindering natural defence mechanisms of cells. In addition, PMSF and TLCK block Clostripain enzymatic activity, while chymostatin leaves it intact. Elevated cytotoxicity of the supernatant is not related to the quantity of high-potassium-sensitive toxin, as was reported previously, since desalted supernatant still exerted its strong toxic effect. Our results show that addition of protease inhibitors for treating diseases complicated by concurrent C. histolyticum infection must require special attention.
Ewa Jankowska-steifer - One of the best experts on this subject based on the ideXlab platform.
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology and Biotechnology, 2011Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains ( r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain ( r = 0.62) and collagenase ( r = 0.87) production, and this effect was statistically significant ( P
-
Vacuolating, lethal, collagenase and Clostripain activities of six reference ATCC Clostridium histolyticum strains
World Journal of Microbiology & Biotechnology, 2010Co-Authors: Ewa Jankowska-steifer, Gayane Martirosian, Alicja Ekiel, Aldona Komar, Barbara Wiechuła, Jarosław Jóźwiak, Paweł Cieślik, Stanislaw MoskalewskiAbstract:We have previously demonstrated that C. histolyticum reference strain ATCC 19401 produces not only lethal factor but also hitherto unrecognized vacuolating toxin. The aim of this study was to compare vacuolating and lethal activities of six reference C. histolyticum strains (ATCC 6282, 8034, 17859, 17860, 19401 and 25770) and to determine whether production of vacuolating toxin is strain-dependent and how the amounts of both toxins produced by the same strain are related to each other and also to protease, collagenase and Clostripain activities. All strains produced vacuolating and lethal toxins as well as collagenase, Clostripain and proteases, but with different yield. Strain ATCC 19401 demonstrated considerable vacuolating and lethal activities and low activity of collagenase, Clostripain and proteases. In other strains such relationship was not evident. Positive correlations were observed in collagenase and Clostripain activities of all studied C. histolyticum strains (r = 0.71). Positive correlations were detected also in vacuolating activities of studied strains and Clostripain (r = 0.62) and collagenase (r = 0.87) production, and this effect was statistically significant (P < 0.05). Negative non-significant correlations were detected: (a) in lethal activities of studied strains and Clostripain, or collagenase activities, (b) in vacuolating activities and protease production.