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Lin Lin - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial mechanism of Clove Oil on Listeria monocytogenes
Food Control, 2018Co-Authors: Haiying Cui, Chenghui Zhang, Lin LinAbstract:Abstract Although Clove Oil is widely used in food preservation, very little is known about its possible antibacterial mechanism. In order to reveal its antibacterial mechanism, the inhibitory effect of Clove Oil on Listeria monocytogenes and its effect on cell membrane, respiratory metabolism, and its interaction with DNA were investigated, respectively. The results showed the treatment of Clove Oil can cause the leakage of three biological macromolecules (protein, ATP and DNA) and the reduction of two intracellular enzymes (β-galactosidase and AKP) activities, indicating that Clove Oil has an effect on cell membrane permeability. The inhibition rate and superposition rate test confirmed that the pathway of Clove Oil affecting the respiratory metabolism of Listeria monocytogenes is TCA pathway. The treatment of Clove Oil can reduce the activity of three key enzymes (isocitrate dehydrogenase, citrate synthase and α-ketoglutarate dehydrogenase) in TCA pathway, and affect the content of metabolites in the pathway. The results of ultraviolet absorption spectroscopy showed that eugenol, the main component of Clove Oil, can change the structure of DNA via the formation of eugenol-DNA chimera.
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the antibacterial activity of Clove Oil chitosan nanoparticles embedded gelatin nanofibers against escherichia coli o157 h7 biofilms on cucumber
International Journal of Food Microbiology, 2018Co-Authors: Haiying Cui, Mei Bai, Marwan M A Rashed, Lin LinAbstract:Abstract This study aims to evaluate the antibacterial activity of Clove Oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of Clove Oil (CO) was 2.5 mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8 h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9 mg/mL gelatin/CO@CNPs treatment for 24 h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6 mg/mL and 9 mg/mL gelatin/CO@CNPs nanofibers treatment at 12 °C for 4 days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for > 4 days.
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synergetic antibacterial efficacy of cold nitrogen plasma and Clove Oil against escherichia coli o157 h7 biofilms on lettuce
Food Control, 2016Co-Authors: Haiying Cui, Lin LinAbstract:Abstract This study aims to evaluate the antimicrobial effects of cold nitrogen plasma (CNP) and Clove Oil against Escherichia coli O157:H7 (E. coli O157:H7) biofilm on lettuce. Both Clove Oil (1 mg/mL, 2 mg/mL and 4 mg/mL) and CNP (400–600 W) displayed significant eradication effect on E. coli O157:H7 biofilms in vitro (p
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The specific antibacterial activity of liposome-encapsulated Clove Oil and its application in tofu
Food Control, 2015Co-Authors: Haiying Cui, Chengting Zhao, Lin LinAbstract:Abstract In this study, the antibacterial activities of Clove Oil and liposome-encapsulated Clove Oil were investigated. First, the antibacterial activity of Clove Oil demonstrated that the essential Oil exhibited favorable antimicrobial activity for both Escherichia coli and Staphylococcus aureus. However, a setback of using Clove Oil as a disinfectant is its low chemical stability. Then Clove Oil was incorporated into a liposome formulation to increase its stability. The optimal polydispersity index (PDI) (0.196), Zeta potential (−24.5 mV) and entrapment efficiency (20.41%) of liposome were obtained at the concentration of Clove Oil to 5.0 mg/mL. In addition, selective antimicrobial activity for S. aureus by utilizing pore-forming toxins (PFTs) to activate Clove Oil release from liposome was observed. By contrast, liposome-encapsulated Clove Oil has no effect on E. coli that doesn't secrete PFTs because antimicrobial component can't reach bacteria. Gas chromatography (GC) assay found that when liposome met S. aureus that secrete PFTs, PFTs would insert into the liposome membranes and form pores, through which the encapsulated Clove Oil was released. Besides, liposome-encapsulated Clove Oil exhibited efficient antimicrobial activity for S. aureus in tofu.
Haiying Cui - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial mechanism of Clove Oil on Listeria monocytogenes
Food Control, 2018Co-Authors: Haiying Cui, Chenghui Zhang, Lin LinAbstract:Abstract Although Clove Oil is widely used in food preservation, very little is known about its possible antibacterial mechanism. In order to reveal its antibacterial mechanism, the inhibitory effect of Clove Oil on Listeria monocytogenes and its effect on cell membrane, respiratory metabolism, and its interaction with DNA were investigated, respectively. The results showed the treatment of Clove Oil can cause the leakage of three biological macromolecules (protein, ATP and DNA) and the reduction of two intracellular enzymes (β-galactosidase and AKP) activities, indicating that Clove Oil has an effect on cell membrane permeability. The inhibition rate and superposition rate test confirmed that the pathway of Clove Oil affecting the respiratory metabolism of Listeria monocytogenes is TCA pathway. The treatment of Clove Oil can reduce the activity of three key enzymes (isocitrate dehydrogenase, citrate synthase and α-ketoglutarate dehydrogenase) in TCA pathway, and affect the content of metabolites in the pathway. The results of ultraviolet absorption spectroscopy showed that eugenol, the main component of Clove Oil, can change the structure of DNA via the formation of eugenol-DNA chimera.
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the antibacterial activity of Clove Oil chitosan nanoparticles embedded gelatin nanofibers against escherichia coli o157 h7 biofilms on cucumber
International Journal of Food Microbiology, 2018Co-Authors: Haiying Cui, Mei Bai, Marwan M A Rashed, Lin LinAbstract:Abstract This study aims to evaluate the antibacterial activity of Clove Oil-loaded chitosan nanoparticles (CO@CNPs) and gelatin electrospun nanofibers against Escherichia coli O157:H7 (E. coli O157:H7) biofilms on cucumbers. The optimal CO@CNPs were prepared when the initial concentration of Clove Oil (CO) was 2.5 mg/mL according to the ionic crosslinking method. CO@CNPs showed high antibacterial activity against E. coli O157:H7 biofilms. After 8 h treatment, almost 99.98% reduction in E. coli O157:H7 population was achieved when CO@CNPs were applied at 30% (w/v). Subsequently, the prepared CO@CNPs were incorporated successfully within gelatin nanofibers by electrospinning. After 9 mg/mL gelatin/CO@CNPs treatment for 24 h, the population of E. coli O157:H7 biofilm reduced by about 99.99% in vitro. Further, the application of gelatin/CO@CNPs nanofibers on cucumber against E. coli O157:H7 biofilm was evaluated as well. After 6 mg/mL and 9 mg/mL gelatin/CO@CNPs nanofibers treatment at 12 °C for 4 days, 4.28 and 4.97 log10 reductions of E. coli O157:H7 biofilm in population were observed, respectively. Finally, the sensory evaluation results implied that the gelatin/CO@CNPs nanofibers treatment could maintain the color and flavor of cucumber well for > 4 days.
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synergetic antibacterial efficacy of cold nitrogen plasma and Clove Oil against escherichia coli o157 h7 biofilms on lettuce
Food Control, 2016Co-Authors: Haiying Cui, Lin LinAbstract:Abstract This study aims to evaluate the antimicrobial effects of cold nitrogen plasma (CNP) and Clove Oil against Escherichia coli O157:H7 (E. coli O157:H7) biofilm on lettuce. Both Clove Oil (1 mg/mL, 2 mg/mL and 4 mg/mL) and CNP (400–600 W) displayed significant eradication effect on E. coli O157:H7 biofilms in vitro (p
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The specific antibacterial activity of liposome-encapsulated Clove Oil and its application in tofu
Food Control, 2015Co-Authors: Haiying Cui, Chengting Zhao, Lin LinAbstract:Abstract In this study, the antibacterial activities of Clove Oil and liposome-encapsulated Clove Oil were investigated. First, the antibacterial activity of Clove Oil demonstrated that the essential Oil exhibited favorable antimicrobial activity for both Escherichia coli and Staphylococcus aureus. However, a setback of using Clove Oil as a disinfectant is its low chemical stability. Then Clove Oil was incorporated into a liposome formulation to increase its stability. The optimal polydispersity index (PDI) (0.196), Zeta potential (−24.5 mV) and entrapment efficiency (20.41%) of liposome were obtained at the concentration of Clove Oil to 5.0 mg/mL. In addition, selective antimicrobial activity for S. aureus by utilizing pore-forming toxins (PFTs) to activate Clove Oil release from liposome was observed. By contrast, liposome-encapsulated Clove Oil has no effect on E. coli that doesn't secrete PFTs because antimicrobial component can't reach bacteria. Gas chromatography (GC) assay found that when liposome met S. aureus that secrete PFTs, PFTs would insert into the liposome membranes and form pores, through which the encapsulated Clove Oil was released. Besides, liposome-encapsulated Clove Oil exhibited efficient antimicrobial activity for S. aureus in tofu.
Inseok Park - One of the best experts on this subject based on the ideXlab platform.
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effects of Clove Oil and lidocaine hcl anesthesia on water parameter during simulated transportation in the marine medaka oryzias dancena
Development & Reproduction, 2017Co-Authors: Inseok Park, Hyun Woo Gil, Yoon Kwon Nam, Tae Ho Lee, Sang Gu Lim, Dong Soo KimAbstract:Optimum concentrations of anesthetic Clove Oil and anesthetic lidocaine-HCl were determined for a species of adult marine medaka, Oryzias dancena, over a range of salinity conditions, and investigated in a transport simulation experiment by analyzing various water and physiological parameters. Research indicated that the higher the concentration of anesthetic at each salinity, the shorter the anesthesia time at each salinity. At each concentration, fish were anesthetized slower at water salinities over 10 ppt (P<0.05). Anesthesia time at 10 ppt was faster than any other salinity. In 10 ppt salinity, the dissolved oxygen (DO) concentrations and respiratory frequencies of the Clove-Oil-administered groups decreased until 48 hours (P<0.05), whereas the NH4+ and CO2 concentrations increased until 48 hours (P<0.05). In same period, the DO, NH4+, and CO2 concentrations and respiratory frequencies all decreased as the Clove Oil concentration increased (P<0.05). The trends in the DO, NH4+, and CO2 concentrations and respiratory frequencies in the lidocaine-HCl-administered groups were similar to those in the Clove-Oil-administered groups. In conclusion, Clove Oil and lidocaine-HCl are effective anesthetics, improving the transportation of the marine medaka. The results from this study will contribute to safe laboratory handling of the marine medaka, which are commonly required by many research studies and experiments.
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Anesthetic effects of Clove Oil and lidocaine-HCl on marine medaka (Oryzias dancena)
Lab Animal, 2011Co-Authors: Inseok Park, Sung Jun Park, Hyun Woo Gil, Yoon Kwon Nam, Dong Soo KimAbstract:Fish may be anesthetized for various experimental and practical purposes, primarily to immobilize them in order to facilitate handling. Marine medaka ( Oryzias dancena ) is a teleost fish used in marine ecotoxicology studies. Despite the importance of anesthesia in handling experimental fish, the effects of anesthesia in marine medaka have not yet been investigated. In this study, the authors evaluated the anesthetic effects (time required for anesthesia to take effect and recovery time) of two anesthetic agents, Clove Oil and lidocaine–HCl, on marine medaka. They anesthetized fish at different water temperatures (23 °C, 26 °C and 29 °C) and using different concentrations of Clove Oil (50 ppm, 75 ppm, 100 ppm, 125 ppm, 150 ppm and 175 ppm) or lidocaine–HCl (300 ppm, 400 ppm, 500 ppm, 600 ppm, 700 ppm and 800 ppm). The time required for anesthesia to take effect decreased significantly as both anesthetic concentration and water temperature increased for both Clove Oil and lidocaine–HCl. To anesthetize marine medaka within approximately 1 min, the optimal concentrations for Clove Oil were 125 ppm at 23 °C, 100 ppm at 26 °C and 75 ppm at 29 °C and for lidocaine–HCl were 800 ppm at 23 °C and 700 ppm at both 26 °C and 29 °C. The authors also compared anesthetic effects in marine medaka of different sizes. Both anesthetic exposure time and recovery time were significantly shorter for smaller fish than for larger fish. These results provide a useful foundation for the laboratory handling of marine medaka.
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efficacy and physiological responses of rock bream oplegnathus fasciatus to anesthetization with Clove Oil
Aquaculture, 2009Co-Authors: Min Ouk Park, Dongwon Seol, Inseok ParkAbstract:Abstract We tested the efficacy (e.g., induction time, recovery time) of Clove Oil as an anesthetic for rock bream Oplegnathus fasciatus . In addition, we also evaluated the physiological response of fish to the anesthetic by measuring plasma cortisol and glucose. In general, fish exposed to higher anesthetic doses were rapidly induced but took longer to recover, while lower water temperatures resulted in longer induction and recovery times. Optimal anesthetic dose and water temperature were estimated to be 150 mgL − 1 at 20 °C, 100 mgL − 1 to 125 mgL − 1 at 24 °C, and 50 mgL − 1 to 75 mgL − 1 at 28 °C. Following the administration of 100 mgL − 1 of Clove Oil at 24 °C, the plasma cortisol level was highest (1.70 ± 0.148 μg/dL) after 1 h while the plasma glucose level was highest (80.0 ± 1.41 mg/dL) after 2 h. It took 2 days for the plasma cortisol and plasma glucose concentrations to return to pre-exposure levels.
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anaesthetic efficacy and physiological responses to Clove Oil anaesthetized kelp grouper epinephelus bruneus
Aquaculture Research, 2008Co-Authors: Min Ouk Park, Woo June Hur, Dongwon Seol, Jinhwan Lee, Inseok ParkAbstract:The efficacy of Clove Oil as an anaesthetic and at producing a physiological response (plasma cortisol and glucose) was evaluated in the kelp grouper, Epinephelus bruneus. To acquire complete anaesthesia in less than 3 min and recovery in <10 min, three doses of Clove Oil were tested at 18, 22 and 26 °C. Although higher anaesthetic doses resulted in shorter induction times and longer recovery times, and a lower temperature resulted in longer anaesthesia induction and slower recovery, we found the optimal dose and administering temperature of Clove Oil to be 250–300 mg L−1 at water temperature of 18 °C, 150–200 mg L−1 at water temperature of 22 °C and 50–100 mg L−1 at water temperature of 26 °C respectively. Following the administration of 150 mg L−1 of Clove Oil at 22 °C, the plasma cortisol level was highest (4.24 ± 1.571 μg dL−1) after 12 h and the plasma glucose was highest (92.7 ± 9.61 mg dL−1) after 2 h. These results should be useful to the aquaculture industry, where anaesthesia is necessary for a range of activities.
David J Chitwood - One of the best experts on this subject based on the ideXlab platform.
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phytotoxicity of Clove Oil to vegetable crop seedlings and nematotoxicity to root knot nematodes
Horttechnology, 2008Co-Authors: Susan L F Meyer, Dilip K Lakshman, Inga A Zasada, Bryan T Vinyard, David J ChitwoodAbstract:ADDITIONAL INDEX WORDS. bio-based nematicide, essential Oil, Meloidogyne incognita, nematode management SUMMARY. Clove Oil derived from the Clove plant [Syzygium aromaticum (=Eugenia caryophyllata)] isactiveagainstvarious sOil-borneplantpathogens andtherefore has potential for use as a bio-based pesticide. A Clove Oil formulation previously found to be toxic to the southern root-knot nematode (Meloidogyne incognita )i n laboratory assays was investigated in greenhouse studies for nematode suppression and phytotoxicity on vegetable crops. Phytotoxicity studies were conducted with 0.1%, 0.2%, and 0.3% Clove Oil applied to sOil 0, 2, 5, and 7 days before transplant of cucumber (Cucumis sativus), muskmelon (Cucumis melo), pepper (Capsicum annuum), and tomato(Solanum lycopersicum) seedlings. Tomato seedlings were the most sensitive to Clove Oil. The 0.2% and 0.3% Clove Oil concentrations applied as drenches at transplant (0 day) were the most phytotoxic to seedlings of all the tested vegetable species, with only 0% to 50% seedling survival. Most of the Clove Oil concentrations applied as drenches at transplant decreased shoot heights and fresh shoot weights of all seedlings. Some applications of Clove Oil at 0.2% and 0.3%, applied 2, 5, or 7 days before transplant also significantly reduced shoot growth, especially of pepper and tomato. Greenhouse experiments evaluating suppression of nematode populations on cucumber were conducted with 0.10%, 0.15%, and 0.20% Clove Oil applied 7 days before transplant. Overall, plants inoculated with nematodes tended to have smaller shoots and heavier roots than plants without nematodes. Effects of Clove Oil treatments on nematode population densities were inconsistent between the two trials. In Trial 1, 0.10% and 0.15% Clove Oil decreased population densities compared with the carrier control. In Trial 2, nematode population densities were lowest in the water and carrier control treatments. The results indicate that, with the tested Clove Oil formulation and application times, southern root-knot nematode populations would not be consistently reduced with Clove Oil concentrations that were not phytotoxic to one or more of the tested vegetable crops.
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dose response effects of Clove Oil from syzygium aromaticum on the root knot nematode meloidogyne incognita
Pest Management Science, 2008Co-Authors: Susan L F Meyer, Dilip K Lakshman, Inga A Zasada, Bryan T Vinyard, David J ChitwoodAbstract:BACKGROUND: Clove Oil, derived from the plant Syzygium aromaticum (L.) Merr. & Perry, is active against various organisms, and was prepared in a soy lecithin/detergent formulation to determine concentrations active against the root-knot nematode Meloidogyne incognita (Kofoid and White) Chitwood. RESULTS: In microwell assays, the mean effective Clove Oil concentration that reduced egg hatch by 50% (EC50) was 0.097% (v/v) Clove Oil; the EC50 for second-stage juvenile (J2) viability was 0.145% Clove Oil (compared with carrier control treatments). Volatiles from 5.0% Clove Oil reduced nematode egg hatch in water by 30%, and decreased viability of hatched J2 by as much as 100%. Reductions were not as large with nematodes in carrier. In sOil trials with J2 recovered from Baermann funnels, the EC50 = 0.192% Clove Oil (compared with water controls). CONCLUSION: The results demonstrated that the tested formulation is active against M. incognita eggs and J2, that the EC50 values for J2 in the microwell studies and the sOil recovery tests were similar to each other and that direct contact with the Clove Oil is needed for optimal management results with this natural product. 2007 Society of Chemical Industry
Juhani Pirhonen - One of the best experts on this subject based on the ideXlab platform.
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efficacy of Clove Oil and ethanol against saprolegnia sp and usability as antifungal agents during incubation of rainbow trout oncorhynchus mykiss walbaum eggs
Aquaculture Research, 2015Co-Authors: Petri Hoskonen, Jouni Heikkinen, Paivi Eskelinen, Juhani PirhonenAbstract:Inhibitory concentrations of Clove Oil and ethanol against growth of Saprolegnia sp. hyphae were screened by a modification of the hemp (Cannabis sativa L.) seed MicroPlate (HeMP) method and their usability as antifungal agents during incubation of rainbow trout Oncorhynchus mykiss eggs was tested. In vitro experiment showed that in continuous static exposure, Clove Oil at 100 mg L−1 significantly inhibited the growth of Saprolegnia, whereas in bath exposures, Clove Oil at 500 mg L−1 had no significant effect at any exposure time tested (15, 60 and 240 min), but Clove Oil at 10 000 mg L−1 significantly inhibited growth at all exposure times. Clove Oil and ethanol treatments had no visible effects on the onset or spread of the fungus during incubation of rainbow trout eggs. Clove Oil at 1000 mg L−1 resulted in 95–100% mortality before the eyed stage was reached. Sublethal concentrations of Clove Oil and ethanol had no effects on the development rate of the embryo or growth and yolk utilization efficiency after hatching. This study suggests that Clove Oil and ethanol may not be options in controlling aquatic fungi infestations during incubation of rainbow trout eggs.
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temperature effects on anaesthesia with Clove Oil in six temperate zone fishes
Journal of Fish Biology, 2004Co-Authors: Petri Hoskonen, Juhani PirhonenAbstract:The potential use of Clove Oil (eugenol) as an anaesthetic for Atlantic salmon Salmo salar, brown trout Salmo trutta, rainbow trout Oncohynchus mykiss, whitefish Coregonus lavaretus, perch Perca fluviatilis and roach Rutilus rutilus was examined at 5, 10, 15 and 20°C using three anaesthetic concentrations (varying from 20 to 200 mg l -1 ) at each temperature. Substantial species differences in sensitivity to Clove Oil were observed, even amongst congeners, and there may be some disadvantages (slow recovery and possibly mortality) with using Clove Oil for 0+ year whitefish and at low temperatures for perch and roach.
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effects of anaesthesia with ms 222 Clove Oil and co2 on feed intake and plasma cortisol in steelhead trout oncorhynchus mykiss
Aquaculture, 2003Co-Authors: Juhani Pirhonen, Carl B SchreckAbstract:We examined the effects tricaine methanesulfonate (MS-222), Clove Oil and CO2 on feed intake and cortisol response in steelhead trout, Oncorhynchus mykiss. Even though a body of literature exists about the effects of different anaesthetics on fish, no comparative information seems to be available about their effects on feed intake after anaesthesia, which would be important to know especially in aquaculture research. We anaesthetised juvenile steelhead trout with these three anaesthetics, and then sampled them 4, 24 and 48 h later. Fish in all groups ate relatively well already 4 h after anaesthesia. However, feed intake in fish treated with Clove Oil or MS-222 was lower than in the controls. There were no differences in feed intake among anaesthetised groups. Plasma cortisol concentrations were elevated 48 h after anaesthetisation, but the treatment means were equal throughout the experiment. Our results support previous findings that Clove Oil is a reasonable alternative to MS-222.