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Lucas Cardoso Marinho - One of the best experts on this subject based on the ideXlab platform.
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plastomes resolve generic limits within tribe clusieae Clusiaceae and reveal the new genus arawakia
Molecular Phylogenetics and Evolution, 2019Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André Márcio Amorim, Liming Cai, Xiaoshan Duan, Brad R Ruhfel, Cassio Van Den Berg, Charles C DavisAbstract:Clusieae is an exclusively Neotropical tribe in the family Clusiaceae sensu stricto. Although tribes within Clusiaceae are morphologically and phylogenetically well-delimited, resolution among genera within these tribes remains elusive. The tribe Clusieae includes an estimated ∼500 species distributed among five genera: Chrysochlamys, Clusia, Dystovomita, Tovomita, and Tovomitopsis. In this study, we used nearly complete plastid genomes from 30 exemplar Clusieae species representing all genera recognized, plus two outgroups to infer the phylogeny of the tribe using Maximum Likelihood and Bayesian Inference. For comparison, we also inferred a phylogeny from the nuclear Internal Transcribed Spacer (ITS) region using the same methods. Our study corroborates earlier findings that Clusia is monophyletic while Tovomita is not. It also provides additional support to the hypothesis that Chrysochlamys and Tovomitopsis are not closely related despite gross morphological similarity. Tovomita is divided into three distantly related clades: (i) core Tovomita (including the type T. guianensis), (ii) T. croatii, and (iii) the T. weddelliana species complex. Members of the T. weddelliana complex are isolated from the core Tovomita, and placed in a well-supported clade that is sister to a clade composed of Chrysochlamys plus Clusia. Tovomita croatii is nested within Chrysochlamys. We propose taxonomic revisions to accommodate our phylogenetic findings, including the description of the new genus Arawakia, which includes the 18 species formerly recognized in the T. weddelliana species complex. Lectotypes are also designated for nine species (i.e., Arawakia angustata, A. lanceolata, A. lingulata, A. longicuneata, A. macrocarpa, A. oblanceolata, A. pithecobia, A. rhizophoroides, and A. weddelliana), and a taxonomic key for the identification of the six genera of Clusieae recognized is presented.
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validation of the name garcinia leptophylla Clusiaceae
Boletim do Museu Paraense Emílio Goeldi - Ciências Naturais, 2018Co-Authors: Volker Bittrich, Lucas Cardoso MarinhoAbstract:Durante a producao do “Catalogo de plantas e fungos do Brasil” (2010), algumas novas combinacoes foram propostasem Garcinia, entretanto, elas sao consideradas invalidas, uma vez que seus respectivos basionimos nao foram indicados.Neste artigo, propomos a validacao do nome Garcinia leptophylla Bittrich, um novo nome apresentado para Rheedialongifolia Planch. & Triana, bem como designamos um lectotipo.
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Resolving the last combination in Tovomitidium , a synonym for Tovomita (Clusiaceae)
Phytotaxa, 2018Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André M. AmorimAbstract:Tovomitidium (Clusiaceae) is a small genus from the Amazon Forest, described to accommodate T. clusiiflorum plus one segregated species from Tovomita , T. speciosum . Some authors considered Tovomitidium as synonymous with Tovomita , but online databases still point to Tovomitidium as a valid genus within the Clusiaceae. Herein, we provide comments on the two species of Tovomitidium and present arguments to reinforce our opinion they belong in Tovomita . Additionally, a new combination, synonym and lectotype are presented.
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Three new species of Tovomita (Clusiaceae) from the Amazon River basin and first record of papillae for Tovomita
Plant Systematics and Evolution, 2016Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, Francisco De Assis Ribeiro Dos Santos, André Márcio AmorimAbstract:Tovomita trachycarpa, T. vismiifolia and T. volkeri (Clusiaceae) are described, illustrated and mapped, and their diagnostic features are compared with those of similar species. Papillae are recorded for the first time for the genus, on the leaf blades, inflorescences and flower buds of T. vismiifolia, giving these structures a ferruginous color. Morphology of pollen grain and selected features of the leaf blade are discussed for the new species.
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The taxonomic significance of pollen morphology in Tovomita Aubl. (Clusiaceae: Clusieae) and related genera
Plant Systematics and Evolution, 2015Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André M. Amorim, Francisco De Assis Ribeiro Dos SantosAbstract:Tovomita (Clusiaceae, tribe Clusieae) comprises about 45 species with Neotropical distribution. Thirty of these species occur in the Brazilian Amazon and Atlantic forest, but have only been poorly sampled in studies of pollen morphology. Pollen grains of ten species of Tovomita and one of Tovomitopsis are characterized and illustrated here and morphologically compared with those from other extant genera of Clusiaceae s.s., as well as the fossil genus Paleoclusia (which shares with Tovomita prolate triaperturate pollen grains with reticulate exines and sexine elements inside the lumina). The pollen grains of the analyzed species of Tovomita were found to be tricolporate, with amb circular to subtriangular, and exine psilate to microreticulate. Tovomita pollen differs markedly from Tovomitopsis in terms of the tectum surface, which is smooth with regular baculate in the former, versus irregular and not smooth, with some twisted baculate processes, in the latter. Other pollen features among species of Clusiaceae are discussed in order to better characterize Tovomita and help elucidate generic limits among related genera.
Pascal Richomme - One of the best experts on this subject based on the ideXlab platform.
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13C-NMR dereplication of Garcinia extracts: Predicted chemical shifts as reliable databases
'Elsevier BV', 2018Co-Authors: A. Bruguière, S. Derbré, C. Coste, Le M. Bot, B. Siegler, S.t. Leong, S.n. Sulaiman, K. Awang, Pascal RichommeAbstract:Usually isolated from Garcinia (Clusiaceae) or Hypericum (Hypericaceae) species, some Polycyclic Polyprenylated AcylPhloroglucinols (PPAPs) have been recently reported as potential research tools for immunotherapy. Aiming at exploring the chemodiversity of PPAPs amongst Garcinia genus, a dereplication process suitable for such natural compounds has been developed. Although less sensitive than mass spectrometry, NMR spectroscopy is perfectly reproducible and allows stereoisomers distinction, justifying the development of 13C-NMR strategies. Dereplication requires the use of databases (DBs). To define if predicted DBs were accurate enough as dereplication tools, experimental and predicted δC of natural products usually isolated from Clusiaceae were compared. The ACD/Labs commercial software allowed to predict 73% of δC in a 1.25 ppm range around the experimental values. Consequently, with these parameters, the major PPAPs from a Garcinia bancana extract were successfully identified using a predicted DB
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Normal phase HPLC-based activity profiling of non-polar crude plant extracts – acetylcholinesterase inhibiting guttiferones from Montrouziera cauliflora as a case study
Natural Product Research, 2016Co-Authors: Anne Landreau, Samuel Bertrand, Laurence Marcourt, Marc Litaudon, David Guilet, Pierre-alain Carrupt, Claudia Simões-pires, Pascal Richomme, Jean-luc WolfenderAbstract:The study describes bioactive compounds as inhibitors of acetylcholinesterase (AChE), from the stem bark extract of Montrouziera cauliflora, selected among 19 dichloromethane extracts from Clusiaceae species. Our work focused on the development of an original normal phase HPLC microfractionation strategy to rapidly assess highly active zones from this crude active non-polar plant extract. Two different microfraction collection methods were evaluated for the assessment of the AChE inhibition. Two guttiferones and a tocotrienol were directly isolated among five compounds identified off-line by NMR after upscaling the fractionation and their AChE inhibition was evaluated. The strengths and weaknesses of the two microfractionation collection methods for HPLC-AChE activity-based profiling are discussed.
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Normal phase HPLC-based activity profiling of non-polar crude plant extracts – acetylcholinesterase inhibiting guttiferones from Montrouziera cauliflora as a case study
Natural Product Research, 2016Co-Authors: Anne Landreau, Samuel Bertrand, Laurence Marcourt, Marc Litaudon, David Guilet, Pierre-alain Carrupt, Claudia Simões-pires, Pascal Richomme, Jean-luc WolfenderAbstract:The study describes bioactive compounds as inhibitors of acetylcholinesterase (AChE), from the stem bark extract of Montrouziera cauliflora, selected among 19 dichloromethane extracts from Clusiaceae species. Our work focused on the development of an original normal phase HPLC microfractionation strategy to rapidly assess highly active zones from this crude active non-polar plant extract. Two different microfraction collection methods were evaluated for the assessment of the AChE inhibition. Two guttiferones and a tocotrienol were directly isolated among five compounds identified off-line by NMR after upscaling the fractionation and their AChE inhibition was evaluated. The strengths and weaknesses of the two microfractionation collection methods for HPLC-AChE activity-based profiling are discussed.
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Anti-inflammatory and immunomodulatory properties of polyphenolic compounds from Clusiaceae/Calophyllaceae: A focus on coumarins
Planta Medica, 2014Co-Authors: Caroline Rouger, Marc Litaudon, Séverine Derbré, Khalijah Awang, Béatrice Charreau, Pascal RichommeAbstract:Vascular endothelium plays a central role in the development of inflammatory and immune processes, which are involved in graft rejection [1]. Many Clusiaceae/Calophyllaceae species (pantropical plants) biosynthesize original polyphenolic compounds exhibiting antioxidant and anti-inflammatory properties [2 – 3]. Therefore, different Clusiaceae/Calophyllaceae polyphenols were selected in order to evaluate in vitro their anti-inflammatory and immunomodulatory potential towards Human Umbilical Vein Endothelial Cells (HUVECs). The VCAM-1, ICAM-1, E-selectin, HLA-I, HLA-II, HLA-E and MICA surface-expressions of HUVECs were evaluated by flow cytometry. It appeared that a few coumarins and one benzophenone at 10µM significantly inhibited the expression of several markers previously induced by TNF-α or IFN-γ cytokines, being more active that the immunosuppressive reference compound – zoledronic acid – at the same concentration. Among these active compounds, one phenylcoumarin isolated from the Malaysian Mesua lepidota T. Anderson (Calophyllaceae) was identified as a new one. By comparing biological effects with substitution patterns, preliminary structure-activity relationship was also established
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Polyphenolic Compounds with Anti -Ages Activity from Three Clusiaceae Plants
European Journal of Medicinal Plants, 2014Co-Authors: Joseph J. Magadula, David Guilet, Séverine Derbré, Zakaria H. Mbwambo, Julia Gatto, Pascal RichommeAbstract:Aim: This study focused on finding molecules with inhibitory effects on Advanced Glycation End-products (AGEs) formation from Tanzanian some Clusiaceae plant species Study Design: Field study and Laboratory experimental tests. Place and Duration of Study: Institute of Traditional Medicine, Muhimbili University of Health and Allied Sciences, P.O. Box 65001, Dar es Salaam, Tanzania and PRES LUNAM, Universite d’Angers, EA 921 SONAS, 16 Bd Daviers, 49045 Angers, France, between June 2011 and July 2013. Methodology: Three Clusiaceae plant species (Garcinia semseii, G. volkensii and Allanblackia ulugurensis) were collected and dried in the field with the assistance of a botanist. Extraction and concentration of plant samples to obtain crude extracts were done in the laboratory following standard procedures. The isolation of the phenolic compounds was carried out by using normal phase column chromatography as well as HighOriginal Research Article European Journal of Medicinal Plants, 4(11): 1336-1344, 2014 1337 performance liquid chromatography (HPLC). The isolated compounds were tested for antiAGE activity using the in vitro automated assay. Results: Two polyphenolic compounds exhibiting phloroglucinol moieties [e.g. polyprenylated benzophenones, such as guttiferone F, 2 (18 mg)] or biflavonoids [such as morelloflavone, 1 (22mg)] were isolated and identified from A. ulugurensis and G. volkensis respectively. The results further indicated that compound 1 is an excellent inhibitor of AGE formation exhibiting an IC50 values of 78 and 64 μM at wavelength of 370/440 (vesperlysines-like AGEs) and 335/385 (pentosidine-like AGEs) respectively. Conclusion: Plants belonging to the Clusiaceae family commonly used in Tanzanian traditional medicine need to be considered as a potential source of molecules exhibiting pharmacological activities such as anti-AGE activity. Morelloflavone (1) and other biflavonoids prove to be very good anti-AGE compounds using our automated screening assay. Hence, our automated in vitro assay allows a fast, effective and quite inexpensive screening of natural compounds and can therefore be applied to high throughput screening projects.
Charles C Davis - One of the best experts on this subject based on the ideXlab platform.
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plastomes resolve generic limits within tribe clusieae Clusiaceae and reveal the new genus arawakia
Molecular Phylogenetics and Evolution, 2019Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André Márcio Amorim, Liming Cai, Xiaoshan Duan, Brad R Ruhfel, Cassio Van Den Berg, Charles C DavisAbstract:Clusieae is an exclusively Neotropical tribe in the family Clusiaceae sensu stricto. Although tribes within Clusiaceae are morphologically and phylogenetically well-delimited, resolution among genera within these tribes remains elusive. The tribe Clusieae includes an estimated ∼500 species distributed among five genera: Chrysochlamys, Clusia, Dystovomita, Tovomita, and Tovomitopsis. In this study, we used nearly complete plastid genomes from 30 exemplar Clusieae species representing all genera recognized, plus two outgroups to infer the phylogeny of the tribe using Maximum Likelihood and Bayesian Inference. For comparison, we also inferred a phylogeny from the nuclear Internal Transcribed Spacer (ITS) region using the same methods. Our study corroborates earlier findings that Clusia is monophyletic while Tovomita is not. It also provides additional support to the hypothesis that Chrysochlamys and Tovomitopsis are not closely related despite gross morphological similarity. Tovomita is divided into three distantly related clades: (i) core Tovomita (including the type T. guianensis), (ii) T. croatii, and (iii) the T. weddelliana species complex. Members of the T. weddelliana complex are isolated from the core Tovomita, and placed in a well-supported clade that is sister to a clade composed of Chrysochlamys plus Clusia. Tovomita croatii is nested within Chrysochlamys. We propose taxonomic revisions to accommodate our phylogenetic findings, including the description of the new genus Arawakia, which includes the 18 species formerly recognized in the T. weddelliana species complex. Lectotypes are also designated for nine species (i.e., Arawakia angustata, A. lanceolata, A. lingulata, A. longicuneata, A. macrocarpa, A. oblanceolata, A. pithecobia, A. rhizophoroides, and A. weddelliana), and a taxonomic key for the identification of the six genera of Clusieae recognized is presented.
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malpighiales phylogenetics gaining ground on one of the most recalcitrant clades in the angiosperm tree of life
American Journal of Botany, 2009Co-Authors: Kenneth J Wurdack, Charles C DavisAbstract:: The eudicot order Malpighiales contains ∼16000 species and is the most poorly resolved large rosid clade. To clarify phylogenetic relationships in the order, we used maximum likelihood, Bayesian, and parsimony analyses of DNA sequence data from 13 gene regions, totaling 15604 bp, and representing all three genomic compartments (i.e., plastid: atpB, matK, ndhF, and rbcL; mitochondrial: ccmB, cob, matR, nad1B-C, nad6, and rps3; and nuclear: 18S rDNA, PHYC, and newly developed low-copy EMB2765). Our sampling of 190 taxa includes representatives from all families of Malpighiales. These data provide greatly increased support for the recent additions of Aneulophus, Bhesa, Centroplacus, Ploiarium, and Rafflesiaceae to Malpighiales; sister relations of Phyllanthaceae + Picrodendraceae, monophyly of Hypericaceae, and polyphyly of Clusiaceae. Oxalidales + Huaceae, followed by Celastrales are successive sisters to Malpighiales. Parasitic Rafflesiaceae, which produce the world's largest flowers, are confirmed as embedded within a paraphyletic Euphorbiaceae. Novel findings show a well-supported placement of Ctenolophonaceae with Erythroxylaceae + Rhizophoraceae, sister-group relationships of Bhesa + Centroplacus, and the exclusion of Medusandra from Malpighiales. New taxonomic circumscriptions include the addition of Bhesa to Centroplacaceae, Medusandra to Peridiscaceae (Saxifragales), Calophyllaceae applied to Clusiaceae subfamily Kielmeyeroideae, Peraceae applied to Euphorbiaceae subfamily Peroideae, and Huaceae included in Oxalidales.
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host to parasite gene transfer in flowering plants phylogenetic evidence from malpighiales
Science, 2004Co-Authors: Charles C Davis, Kenneth J WurdackAbstract:Horizontal gene transfer (HGT) between sexually unrelated species has recently been documented for higher plants, but mechanistic explanations for HGTs have remained speculative. We show that a parasitic relationship may facilitate HGT between flowering plants. The endophytic parasites Rafflesiaceae are placed in the diverse order Malpighiales. Our multigene phylogenetic analyses of Malpighiales show that mitochrodrial ( matR ) and nuclear loci (18 S ribosomal DNA and PHYC ) place Rafflesiaceae in Malpighiales, perhaps near Ochnaceae/Clusiaceae. Mitochondrial nad1B-C , however, groups them within Vitaceae, near their obligate host Tetrastigma . These discordant phylogenetic hypotheses strongly suggest that part of the mitochondrial genome in Rafflesiaceae was acquired via HGT from their hosts.
Pedro Fiaschi - One of the best experts on this subject based on the ideXlab platform.
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plastomes resolve generic limits within tribe clusieae Clusiaceae and reveal the new genus arawakia
Molecular Phylogenetics and Evolution, 2019Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André Márcio Amorim, Liming Cai, Xiaoshan Duan, Brad R Ruhfel, Cassio Van Den Berg, Charles C DavisAbstract:Clusieae is an exclusively Neotropical tribe in the family Clusiaceae sensu stricto. Although tribes within Clusiaceae are morphologically and phylogenetically well-delimited, resolution among genera within these tribes remains elusive. The tribe Clusieae includes an estimated ∼500 species distributed among five genera: Chrysochlamys, Clusia, Dystovomita, Tovomita, and Tovomitopsis. In this study, we used nearly complete plastid genomes from 30 exemplar Clusieae species representing all genera recognized, plus two outgroups to infer the phylogeny of the tribe using Maximum Likelihood and Bayesian Inference. For comparison, we also inferred a phylogeny from the nuclear Internal Transcribed Spacer (ITS) region using the same methods. Our study corroborates earlier findings that Clusia is monophyletic while Tovomita is not. It also provides additional support to the hypothesis that Chrysochlamys and Tovomitopsis are not closely related despite gross morphological similarity. Tovomita is divided into three distantly related clades: (i) core Tovomita (including the type T. guianensis), (ii) T. croatii, and (iii) the T. weddelliana species complex. Members of the T. weddelliana complex are isolated from the core Tovomita, and placed in a well-supported clade that is sister to a clade composed of Chrysochlamys plus Clusia. Tovomita croatii is nested within Chrysochlamys. We propose taxonomic revisions to accommodate our phylogenetic findings, including the description of the new genus Arawakia, which includes the 18 species formerly recognized in the T. weddelliana species complex. Lectotypes are also designated for nine species (i.e., Arawakia angustata, A. lanceolata, A. lingulata, A. longicuneata, A. macrocarpa, A. oblanceolata, A. pithecobia, A. rhizophoroides, and A. weddelliana), and a taxonomic key for the identification of the six genera of Clusieae recognized is presented.
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Resolving the last combination in Tovomitidium , a synonym for Tovomita (Clusiaceae)
Phytotaxa, 2018Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André M. AmorimAbstract:Tovomitidium (Clusiaceae) is a small genus from the Amazon Forest, described to accommodate T. clusiiflorum plus one segregated species from Tovomita , T. speciosum . Some authors considered Tovomitidium as synonymous with Tovomita , but online databases still point to Tovomitidium as a valid genus within the Clusiaceae. Herein, we provide comments on the two species of Tovomitidium and present arguments to reinforce our opinion they belong in Tovomita . Additionally, a new combination, synonym and lectotype are presented.
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Three new species of Tovomita (Clusiaceae) from the Amazon River basin and first record of papillae for Tovomita
Plant Systematics and Evolution, 2016Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, Francisco De Assis Ribeiro Dos Santos, André Márcio AmorimAbstract:Tovomita trachycarpa, T. vismiifolia and T. volkeri (Clusiaceae) are described, illustrated and mapped, and their diagnostic features are compared with those of similar species. Papillae are recorded for the first time for the genus, on the leaf blades, inflorescences and flower buds of T. vismiifolia, giving these structures a ferruginous color. Morphology of pollen grain and selected features of the leaf blade are discussed for the new species.
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The taxonomic significance of pollen morphology in Tovomita Aubl. (Clusiaceae: Clusieae) and related genera
Plant Systematics and Evolution, 2015Co-Authors: Lucas Cardoso Marinho, Pedro Fiaschi, André M. Amorim, Francisco De Assis Ribeiro Dos SantosAbstract:Tovomita (Clusiaceae, tribe Clusieae) comprises about 45 species with Neotropical distribution. Thirty of these species occur in the Brazilian Amazon and Atlantic forest, but have only been poorly sampled in studies of pollen morphology. Pollen grains of ten species of Tovomita and one of Tovomitopsis are characterized and illustrated here and morphologically compared with those from other extant genera of Clusiaceae s.s., as well as the fossil genus Paleoclusia (which shares with Tovomita prolate triaperturate pollen grains with reticulate exines and sexine elements inside the lumina). The pollen grains of the analyzed species of Tovomita were found to be tricolporate, with amb circular to subtriangular, and exine psilate to microreticulate. Tovomita pollen differs markedly from Tovomitopsis in terms of the tectum surface, which is smooth with regular baculate in the former, versus irregular and not smooth, with some twisted baculate processes, in the latter. Other pollen features among species of Clusiaceae are discussed in order to better characterize Tovomita and help elucidate generic limits among related genera.
João Henrique G. Lago - One of the best experts on this subject based on the ideXlab platform.
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Calanolides E1 and E2, two related coumarins from Calophyllum brasiliense Cambess. (Clusiaceae), displayed in vitro activity against amastigote forms of Trypanosoma cruzi and Leishmania infantum.
Natural product research, 2020Co-Authors: Luciana Grus M. Silva, Kaio S. Gomes, Thais A. Costa-silva, Maiara M. Romanelli, Andre G. Tempone, Patricia Sartorelli, João Henrique G. LagoAbstract:In the present work, the MeOH extract from stem barks of Calophyllum brasiliense Cambess. (Clusiaceae) displayed activity against amastigote forms of Trypanosoma cruzi and Leishmania infantum and w...