The Experts below are selected from a list of 57 Experts worldwide ranked by ideXlab platform

Iuis Who Subcommittee On Cd Nomenclature - One of the best experts on this subject based on the ideXlab platform.

Tatiana Miranda Deliberador - One of the best experts on this subject based on the ideXlab platform.

  • leukocyte platelet rich plasma l prp induces an abnormal histophenotype in craniofacial bone repair associated with changes in the immunopositivity of the hematopoietic Clusters of Differentiation osteoproteins and tgf β1
    Clinical Implant Dentistry and Related Research, 2014
    Co-Authors: Allan Fernando Giovanini, Joao Ricardo Almeida Grossi, Carla Castiglia Gonzaga, Joao Cesar Zielak, Isabella Gohringer, Juliana Souza Vieira, Juliane Kuczera, Marco Antonio De Oliveira Filho, Tatiana Miranda Deliberador
    Abstract:

    BACKGROUND: Leukocyte-platelet-rich plasma (L-PRP) is considered an important source of growth factors, especially Transforming growth factor β 1 (TGF-β1), which modulates the proliferation and regulation of mesenchymal cells, and also exerts an influence on the hematopoiesis, osteogenesis, and adipogenesis in bone microenvironment. Thus, the aim of this study was to evaluate the effect of L-PRP on the calvarial bone repair and compare its results on the presence of TGF-β1, CD34, CD45, bone morphogenetic protein 2 (BMP2), BMPR1B, and Runx2 proteins detected by immunohistochemistry. MATERIAL AND METHODS: Four bone defects were created on the calvaria of 23 rabbits. The defects were treated with autograft, L-PRP alone, and L-PRP mixed with autograft. The animals were euthanized at 2, 4, and 6 weeks post-surgery. RESULTS: Unlike autograft and sham groups, the defects treated with L-PRP demonstrated significant positivity to TGF-β1, while the BMP2 was scarce. These results coincided with the lower bone matrix deposited and larger medullary area, which were composed of fibrosis, when treated with only L-PRP, or intense adiposity on defects filled with L-PRP mixed with autograft. The fibrosis that occurred was associated with a minor percentage of osteoproteins, intense presence of CD34(+) CD45(-) cells, and significant expression of TGF-β1 in all time periods analyzed. The adiposity occurred from the major presence of osteoprogenitor BMPR1B (+) Runx2(+) cells simultaneously to BMP2(-) TGF-β1(+) and CD34(+) CD45(+/-) expressions predominantly on the earlier period. CONCLUSION: From this study, it can be concluded that the L-PRP used alone or mixed to autograft hindered the osteoneogenesis due to suppression of immunoexpression of BMP2, while the immunopositivity of TGF-β1 was intense. When used alone, the L-PRP induced a fibrotic condition associated with TGF-β1 presence and lack of osteoproteins, but when L-PRP was mixed to autograft, it induced the presence of the osteolineage cells (BMPR1B (+) Runx2(+) ), but also inhibited the terminal osteoblastic maturation associated with the lack of BMP2 and the presence of TGF-β1(+) , a fact that contributed to cellular transDifferentiation into fat cells.

S H Park - One of the best experts on this subject based on the ideXlab platform.

  • thu0532 estrogen upregulates interleukin 21 production of Clusters of Differentiation 4 positive t lymphocytes in patients with systemic lupus erythematosus
    Annals of the Rheumatic Diseases, 2014
    Co-Authors: J Y Kang, Seunghyun Jung, S K Kwok, J H Ju, Kyungsu Park, S H Park
    Abstract:

    Background Systemic lupus erythematosus (SLE) is an autoimmune disease in which various organs and tissues are damaged through abnormal immune responses mediated by tissue-binding autoantibodies and immune complex deposition. As the majority of SLE patients are women of child-bearing age, estrogen has been suggested to play an important role in the pathogenesis of SLE. One of the proposed roles of estrogen is to increase autoantibody production. IL-21, a common-γ chain cytokine, has been shown to be crucial in the Differentiation of activated B cells into plasma cells. Objectives Based on these concepts, we investigated the effect of estrogen on the production of IL-21 by T cells and subsequent B cell activation in SLE patients. Methods Peripheral blood mononuclear cells (PBMCs) were obtained from peripheral blood of 23 SLE patients and 16 healthy controls. CD4+ T cells, non CD4+ T cells and B cells were isolated using microbeads. Isolated cells were treated with 17-β estradiol at various concentrations for 48hrs. The expression of IL-21 and its receptor was assessed by measuring the level of protein and mRNA using ELISA and RT-PCR, respectively. The level of immunoglobulin G was measured with specific ELISA. Results The expression of IL-21 and its receptor in serum, PBMCs, and CD4+ T cells were higher in the patients with SLE compared to healthy controls. Exposure of CD4+ T cells from SLE patients to 17-β estradiol leads to a dose-and time-dependent increase in the IL-21 expression. The increase was abolished in the presence of MAP kinase (MEK, p38, JNK) inhibitors. B cells of healthy controls showed an increased antibody production when they were co-cultured with estrogen treated CD4+ T cells of patients with SLE. Treatment with anti-IL-21 antibody abrogated the increased antibody production of the co-culture systems, suggesting the increase was mediated by IL-21 dependent manner. Conclusions Estrogen upregulates IL-21 expression of CD4+ T cells via MAPK dependent pathways in SLE patients, which in turn induces increased antibody production by B cells. Disclosure of Interest : None declared DOI 10.1136/annrheumdis-2014-eular.3160

M K Breitenstein - One of the best experts on this subject based on the ideXlab platform.

  • labels of aberrant Clusters of Differentiation gene expression in a compendium of systemic lupus erythematosus patients
    bioRxiv, 2018
    Co-Authors: Trang T Le, N O Blackwood, M K Breitenstein
    Abstract:

    This author manuscript serves as an extended annotation of gene expression for all known Clusters of Differentiation (CD) within a compendium of systemic lupus erythematosus (SLE) patients. The overarching goal for this line of research is to enrich the CD transcriptome with perspectives of upstream gene expression features. Within our study, CD gene expression features (n=351) were labeled as 'aberrant' or 'non-aberrant, with 'aberrant' being further delineated as 'low' or 'high'. Gene expression was stratified at 20 and 80 percentiles, with low expression being between 0 and 20, average between 20 and 80, and high between 80 and 100. Some CDs required minor shifting of two-tailed thresholds, while others required binary transformation to characterize the CD feature. Detailed labelling of aberrance for all CD features (n=351) identified within the SLE Compendium is provided, including histograms of CD gene expression distribution and descriptive statistics of expression and variation.

  • integrated machine learning pipeline for aberrant biomarker enrichment i mab characterizing Clusters of Differentiation within a compendium of systemic lupus erythematosus patients
    American Medical Informatics Association Annual Symposium, 2018
    Co-Authors: Trang T Le, N O Blackwood, Jaclyn N Taroni, Weixuan Fu, M K Breitenstein
    Abstract:

    : Clusters of Differentiation (CD) are cell surface biomarkers that denote key biological differences between cell types and disease state. CD-targeting therapeutic monoclonal antibodies (mABs) afford rich trans-disease repositioning opportunities. Within a compendium of systemic lupus erythematous (SLE) patients, we applied the Integrated machine learning pipeline for aberrant biomarker enrichment (i-mAB) to profile de novo gene expression features affecting CD20, CD22 and CD30 gene aberrance. First, a novel Relief-based algorithm identified interdependent features(p=681) predicting treatment-naive SLE patients (balanced accuracy=0.822). We then compiled CD-associated expression profiles using regularized logistic regression and pathway enrichment analyses. On an independent general cell line model system data, we replicated associations (in silico) of BCL7A (padj=1.69e-9) and STRBP(padj=4.63e-8) with CD22; NCOA2(padj=7.00e-4), ATN1 (padj=1.71e-2), and HOXC4(padj=3.34e-2) with CD30; and PHOSPHO1, a phosphatase linked to bone mineralization, with both CD22(padj=4.37e-2) and CD30(padj=7.40e-3). Utilizing carefully aggregated secondary data and leveraging a priori hypotheses, i-mAB fostered robust biomarker profiling among interdependent biological features.

Chen Tingmei - One of the best experts on this subject based on the ideXlab platform.

  • effect of total saponins of panax ginseng on k562 cells in expressing Clusters of Differentiation antigen and hematopoietic growth factor recepters
    Journal of Chongqing Medical University, 2003
    Co-Authors: Chen Tingmei
    Abstract:

    Objective:To clarify further the mechanisms for total saponins of Panax ginseng(TSPG)in inducing Differentiation of K562 cells,and to provide the theoretical basis and the experimental evidence of its clinical application.Methods:By using cell culture in vitro,morphological observation and flow cytometry,the effect of TSPG on K562 cells in expressing CD 15 ,HIR 2,EPO-R and GM-CSF-R were studied.Results:The results indicated that TSPG could induce the Differentiation of K562 cells toward proerythroid cells and progranulocytic cells.It was also shown that after induction by TSPG,the ratio of positive K562 cells expressing CD 15 ,HIR 2,erythropoietin recepter(EPO-R) and granulocyte-macrophage colony stimulating facter recepter(GM-CSF-R) increased.Conclusion:K562 cells are abnormal cells whose Differentiation are blocked,but its biological features are similar to the normal hematopoietic stem cells.The mechanism for TSPG inducing Differentiation of K562 cells may be related to the higher expression of EPO-R and GM-CSF-R.