The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Shinji Yamasaki - One of the best experts on this subject based on the ideXlab platform.
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integron bearing methicillin resistant Coagulase Negative Staphylococci in south china 2001 2004
Fems Microbiology Letters, 2008Co-Authors: Zhenbo Xu, M J Alam, Lin Li, Shinji YamasakiAbstract:A total of 53 methicillin-resistant Coagulase-Negative Staphylococci strains isolated in a hospital in Guangzhou, China, were analyzed to detect class 1 integrons and SCC mec typing. Thirty strains had the class 1 integrase ( intI1 ) gene and 26 strains possessed the 3′ conserved region of qacE Δ 1 - sul1 . Four different types of gene cassette arrays were found and a highly prevalent array of dfrA12-orfF-aadA2 gene cassettes was observed. Thirty class 1 integron-positive Coagulase-Negative Staphylococci strains were subjected to Southern hybridization analysis; the result showed that class 1 integrons were located on chromosome, not plasmid. According to the results of SCC mec typing for 30 integron-bearing MRCNS strains, five, 15 and five strains belonged to type I, II and III SCC mec , respectively, and five strains were untypeable. For 23 non-integron-bearing methicillin-resistant Coagulase-Negative Staphylococci strains, four, nine and seven strains belonged to type I, II and III SCC mec , respectively, and three strains were untypeable. None of the strains belonged to type IV or V. Twenty-three Coagulase-Negative Staphylococci isolates of three Staphylococcal species that contained the dfrA12-orfF-aadA2 gene cassette array were phylogenetically unrelated to each other by randomly amplified polymorphic DNA, indicating that the gene cassettes might be disseminated in the clinical strains by a horizontal gene transfer.
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integron bearing methicillin resistant Coagulase Negative Staphylococci in south china 2001 2004
Fems Microbiology Letters, 2008Co-Authors: M J Alam, Shinji Yamasaki, Lei ShiAbstract:A total of 53 methicillin-resistant Coagulase-Negative Staphylococci strains isolated in a hospital in Guangzhou, China, were analyzed to detect class 1 integrons and SCCmec typing. Thirty strains had the class 1 integrase (intI1) gene and 26 strains possessed the 3' conserved region of qacEDelta1-sul1. Four different types of gene cassette arrays were found and a highly prevalent array of dfrA12-orfF-aadA2 gene cassettes was observed. Thirty class 1 integron-positive Coagulase-Negative Staphylococci strains were subjected to Southern hybridization analysis; the result showed that class 1 integrons were located on chromosome, not plasmid. According to the results of SCCmec typing for 30 integron-bearing MRCNS strains, five, 15 and five strains belonged to type I, II and III SCCmec, respectively, and five strains were untypeable. For 23 non-integron-bearing methicillin-resistant Coagulase-Negative Staphylococci strains, four, nine and seven strains belonged to type I, II and III SCCmec, respectively, and three strains were untypeable. None of the strains belonged to type IV or V. Twenty-three Coagulase-Negative Staphylococci isolates of three Staphylococcal species that contained the dfrA12-orfF-aadA2 gene cassette array were phylogenetically unrelated to each other by randomly amplified polymorphic DNA, indicating that the gene cassettes might be disseminated in the clinical strains by a horizontal gene transfer.
Issam I Raad - One of the best experts on this subject based on the ideXlab platform.
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can procalcitonin differentiate staphylococcus aureus from Coagulase Negative Staphylococci in clustered gram positive bacteremia
Diagnostic Microbiology and Infectious Disease, 2013Co-Authors: William Shomali, Ray Y Hachem, Annemarie P Chaftari, Ramez Bahu, Gilbert El Helou, Ying Jiang, Alexander N Hanania, Ruth Reitzel, Issam I RaadAbstract:Procalcitonin (PCT) and pro-adrenomedullin (ProADM) have been proposed as diagnostic and prognostic biomarkers of infection. Between July 2009 and January 2012, we studied the role of these biomarkers in 163 patients with clustered gram-positive and gram-Negative bacteremia. PCT levels were significantly higher in patients with Staphylococcus aureus and gram-Negative bacteremia than those with Coagulase-Negative Staphylococci (CoNS) isolated from blood cultures (P = 0.29 and <0.001, respectively). ProADM levels were only significantly higher in patients with gram-Negative bacteremia (median 1.46 nmol/L) than those with CoNS (median 1.01 nmol/L) (P = 0.04). Among patients with CoNS, PCT, and ProADM, levels failed to differentiate blood contamination (medians 0.24 ng/mL and 0.97 nmol/L) from true bacteremia (medians 0.26 ng/mL and 1.14 nmol/L) (P = 0.51 and 0.57, respectively). In cancer patients, PCT (and to a lesser extent, ProADM) was useful in differentiating CoNS from S. aureus and gram-Negative bacteremia.
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can procalcitonin differentiate staphylococcus aureus from Coagulase Negative Staphylococci in clustered gram positive bacteremia
Diagnostic Microbiology and Infectious Disease, 2013Co-Authors: William Shomali, Ray Y Hachem, Annemarie P Chaftari, Ramez Bahu, Gilbert El Helou, Ying Jiang, Alexander N Hanania, Ruth Reitzel, Issam I RaadAbstract:Abstract Procalcitonin (PCT) and pro-adrenomedullin (ProADM) have been proposed as diagnostic and prognostic biomarkers of infection. Between July 2009 and January 2012, we studied the role of these biomarkers in 163 patients with clustered gram-positive and gram-Negative bacteremia. PCT levels were significantly higher in patients with Staphylococcus aureus and gram-Negative bacteremia than those with Coagulase-Negative Staphylococci (CoNS) isolated from blood cultures ( P = 0.29 and P = 0.04). Among patients with CoNS, PCT, and ProADM, levels failed to differentiate blood contamination (medians 0.24 ng/mL and 0.97 nmol/L) from true bacteremia (medians 0.26 ng/mL and 1.14 nmol/L) ( P = 0.51 and 0.57, respectively). In cancer patients, PCT (and to a lesser extent, ProADM) was useful in differentiating CoNS from S . aureus and gram-Negative bacteremia.
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comparing clinical and microbiological methods for the diagnosis of true bacteraemia among patients with multiple blood cultures positive for Coagulase Negative Staphylococci
Clinical Microbiology and Infection, 2011Co-Authors: Al I Wohoush, J Rivera, Javier Cairo, Ray Y Hachem, Issam I RaadAbstract:Abstract We assessed the accuracy of the Centers for Disease Control and Prevention (CDC) clinical criteria as well as other microbiological methods for the diagnosis of Coagulase-Negative Staphylococci bacteraemia. The CDC clinical criteria had low accuracy, which can be improved by speciation, particularly if the patient had more than two positive blood cultures.
Lei Shi - One of the best experts on this subject based on the ideXlab platform.
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integron bearing methicillin resistant Coagulase Negative Staphylococci in south china 2001 2004
Fems Microbiology Letters, 2008Co-Authors: M J Alam, Shinji Yamasaki, Lei ShiAbstract:A total of 53 methicillin-resistant Coagulase-Negative Staphylococci strains isolated in a hospital in Guangzhou, China, were analyzed to detect class 1 integrons and SCCmec typing. Thirty strains had the class 1 integrase (intI1) gene and 26 strains possessed the 3' conserved region of qacEDelta1-sul1. Four different types of gene cassette arrays were found and a highly prevalent array of dfrA12-orfF-aadA2 gene cassettes was observed. Thirty class 1 integron-positive Coagulase-Negative Staphylococci strains were subjected to Southern hybridization analysis; the result showed that class 1 integrons were located on chromosome, not plasmid. According to the results of SCCmec typing for 30 integron-bearing MRCNS strains, five, 15 and five strains belonged to type I, II and III SCCmec, respectively, and five strains were untypeable. For 23 non-integron-bearing methicillin-resistant Coagulase-Negative Staphylococci strains, four, nine and seven strains belonged to type I, II and III SCCmec, respectively, and three strains were untypeable. None of the strains belonged to type IV or V. Twenty-three Coagulase-Negative Staphylococci isolates of three Staphylococcal species that contained the dfrA12-orfF-aadA2 gene cassette array were phylogenetically unrelated to each other by randomly amplified polymorphic DNA, indicating that the gene cassettes might be disseminated in the clinical strains by a horizontal gene transfer.
Satu Pyorala - One of the best experts on this subject based on the ideXlab platform.
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Coagulase Negative Staphylococci emerging mastitis pathogens
Veterinary Microbiology, 2009Co-Authors: Satu Pyorala, Suvi TaponenAbstract:Coagulase-Negative Staphylococci (CNS) have become the most common bovine mastitis isolate in many countries and could therefore be described as emerging mastitis pathogens. The prevalence of CNS mastitis is higher in primiparous cows than in older cows. CNS are not as pathogenic as the other principal mastitis pathogens and infection mostly remains subclinical. However, CNS can cause persistent infections, which result in increased milk somatic cell count (SCC) and decreased milk quality. CNS infection can damage udder tissue and lead to decreased milk production. Staphylococcus simulans and Staphylococcus chromogenes are currently the predominant CNS species in bovine mastitis. S. chromogenes is the major CNS species affecting nulliparous and primiparous cows whereas S. simulans has been isolated more frequently from older cows. Multiparous cows generally become infected with CNS during later lactation whereas primiparous cows develop infection before or shortly after calving. CNS mastitis is not a therapeutic problem as cure rates after antimicrobial treatment are usually high. Based on current knowledge, it is difficult to determine whether CNS species behave as contagious or environmental pathogens. Control measures against contagious mastitis pathogens, such as post-milking teat disinfection, reduce CNS infections in the herd. Phenotypic methods for identification of CNS are not sufficiently reliable, and molecular methods may soon replace them. Knowledge of the CNS species involved in bovine mastitis is limited. The dairy industry would benefit from more research on the epidemiology of CNS mastitis and more reliable methods for species identification.
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Coagulase Negative Staphylococci as cause of bovine mastitis not so different from staphylococcus aureus
Veterinary Microbiology, 2009Co-Authors: Suvi Taponen, Satu PyoralaAbstract:In this review of the literature, mastitis-causing Coagulase-Negative Staphylococci (CNS) and Staphylococcus aureus are compared. Staphylococci are the bacteria most commonly isolated from bovine mastitis, and CNS are now predominant over S. aureus in most countries. CNS include various species, but only a few prevail in bovine mastitis. S. aureus can cause clinical mastitis, but often causes subclinical mastitis, which remains persistent and increases milk somatic cell count. CNS, traditionally regarded as minor pathogens, seem to lack the ability to cause severe mastitis. CNS can, however, persist in the mammary gland and moderately increase milk somatic cell count. Resistance to various antimicrobials is more common in CNS than in S. aureus, but CNS mastitis responds much better to antimicrobial treatment than S. aureus mastitis.
M J Alam - One of the best experts on this subject based on the ideXlab platform.
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integron bearing methicillin resistant Coagulase Negative Staphylococci in south china 2001 2004
Fems Microbiology Letters, 2008Co-Authors: Zhenbo Xu, M J Alam, Lin Li, Shinji YamasakiAbstract:A total of 53 methicillin-resistant Coagulase-Negative Staphylococci strains isolated in a hospital in Guangzhou, China, were analyzed to detect class 1 integrons and SCC mec typing. Thirty strains had the class 1 integrase ( intI1 ) gene and 26 strains possessed the 3′ conserved region of qacE Δ 1 - sul1 . Four different types of gene cassette arrays were found and a highly prevalent array of dfrA12-orfF-aadA2 gene cassettes was observed. Thirty class 1 integron-positive Coagulase-Negative Staphylococci strains were subjected to Southern hybridization analysis; the result showed that class 1 integrons were located on chromosome, not plasmid. According to the results of SCC mec typing for 30 integron-bearing MRCNS strains, five, 15 and five strains belonged to type I, II and III SCC mec , respectively, and five strains were untypeable. For 23 non-integron-bearing methicillin-resistant Coagulase-Negative Staphylococci strains, four, nine and seven strains belonged to type I, II and III SCC mec , respectively, and three strains were untypeable. None of the strains belonged to type IV or V. Twenty-three Coagulase-Negative Staphylococci isolates of three Staphylococcal species that contained the dfrA12-orfF-aadA2 gene cassette array were phylogenetically unrelated to each other by randomly amplified polymorphic DNA, indicating that the gene cassettes might be disseminated in the clinical strains by a horizontal gene transfer.
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integron bearing methicillin resistant Coagulase Negative Staphylococci in south china 2001 2004
Fems Microbiology Letters, 2008Co-Authors: M J Alam, Shinji Yamasaki, Lei ShiAbstract:A total of 53 methicillin-resistant Coagulase-Negative Staphylococci strains isolated in a hospital in Guangzhou, China, were analyzed to detect class 1 integrons and SCCmec typing. Thirty strains had the class 1 integrase (intI1) gene and 26 strains possessed the 3' conserved region of qacEDelta1-sul1. Four different types of gene cassette arrays were found and a highly prevalent array of dfrA12-orfF-aadA2 gene cassettes was observed. Thirty class 1 integron-positive Coagulase-Negative Staphylococci strains were subjected to Southern hybridization analysis; the result showed that class 1 integrons were located on chromosome, not plasmid. According to the results of SCCmec typing for 30 integron-bearing MRCNS strains, five, 15 and five strains belonged to type I, II and III SCCmec, respectively, and five strains were untypeable. For 23 non-integron-bearing methicillin-resistant Coagulase-Negative Staphylococci strains, four, nine and seven strains belonged to type I, II and III SCCmec, respectively, and three strains were untypeable. None of the strains belonged to type IV or V. Twenty-three Coagulase-Negative Staphylococci isolates of three Staphylococcal species that contained the dfrA12-orfF-aadA2 gene cassette array were phylogenetically unrelated to each other by randomly amplified polymorphic DNA, indicating that the gene cassettes might be disseminated in the clinical strains by a horizontal gene transfer.