The Experts below are selected from a list of 6519 Experts worldwide ranked by ideXlab platform
Hyun S Lillehoj - One of the best experts on this subject based on the ideXlab platform.
-
induction of protective immunity against experimental eimeria tenella infection using serum exosomes
Veterinary Parasitology, 2016Co-Authors: Emilio Del Cacho, Hyun S Lillehoj, Erik P Lillehoj, Margarita Gallego, Joaquin Quilez, Caridad SanchezacedoAbstract:Abstract Avian Coccidiosis is caused by Eimeria , a unicellular, apicomplexan protist which primarily infects intestinal epithelia resulting in nutrient malabsorption and reduced growth of commercial poultry. Vaccination of chickens with exosomes isolated from antigen presenting cells containing parasite antigens (Ags) represents a promising alternative strategy to control avian Coccidiosis, but is restricted in its commercial application due to limitations on production scale-up for mass immunization programs. Here, we report the biochemical and physiologic characteristics of exosomes derived from serum of Eimeria tenella -infected chickens and their feasibility for inducing protective immunity to experimental Coccidiosis. Exosomes isolated from the serum of E. tenella -infected chickens contained a subset of protein Ags found in the intact parasite. Serum-derived exosomes containing these E. tenella Ags localized to the intestine and spleen following intramuscular injection into naive chickens. In vitro ELISPOT assays revealed increased numbers of IL-2-, IL-4-, IL-6-, and IFN-γ-secreting cells in the intestine and spleen of exosome-administered chickens, compared with vehicle controls. Pre-immunization of chickens with serum exosomes from E. tenella -infected chickens increased both body weight gain and feed conversion efficiency, and reduced both fecal parasite shedding and gut lesion scores following parasite infection, compared with vehicle controls. Finally, immunization with CD80 + serum exosomes stimulated greater numbers of cytokine-producing cells, and higher levels of protective immunity to E. tenella infection, compared with CD80 − exosomes. These results suggest the possibility of producing an effective, parasite-free vaccine against avian Coccidiosis under field conditions using serum-derived CD80 + exosomes containing parasite Ags.
-
protective effect of hyperimmune egg yolk igy antibodies against eimeria tenella and eimeria maxima infections
Veterinary Parasitology, 2009Co-Authors: Sunghyen Lee, Hyun S Lillehoj, Seung I Jang, D W Park, A Morales, D Garcia, E Lucio, R Larios, G Victoria, D MarrufoAbstract:Avian Coccidiosis is caused by several distinct species of Eimeria protozoa and is the major parasitic disease of poultry of economic importance. As an alternative strategy to control avian Coccidiosis without using prophylactic medications, we have investigated the efficacy of inducing passive immunity against Coccidiosis by orally feeding hyperimmune IgY antibodies. In this study, a commercially available egg yolk powder, Supracox (SC), a purified IgY fraction of egg yolk prepared from hens hyperimmunized with three major species of Eimeria oocysts, were continuously fed to young chicks from hatch. Upon orally infecting these broiler chicks with Eimeria tenella and Eimeria maxima oocysts at 1 week of age, they showed significantly higher body weight gains (P<0.05) compared to the untreated controls. Furthermore, SC-fed chicks showed significantly less intestinal lesions and reduced fecal oocyst output compared to the untreated controls following oral infections with E. tenella and E. maxima. These results provide clear evidence that passive immunization of chicks with hyperimmune egg yolk IgY antibodies provide significant protection against E. tenella or E. maxima infections.
-
poultry Coccidiosis recent advancements in control measures and vaccine development
Expert Review of Vaccines, 2006Co-Authors: Rami A Dalloul, Hyun S LillehojAbstract:Coccidiosis is recognized as the major parasitic disease of poultry and is caused by the apicomplexan protozoan Eimeria. Coccidiosis seriously impairs the growth and feed utilization of infected animals resulting in loss of productivity. Conventional disease control strategies rely heavily on chemoprophylaxis and, to a certain extent, live vaccines. Combined, these factors inflict tremendous economic losses to the world poultry industry in excess of USD 3 billion annually. Increasing regulations and bans on the use of anticoccidial drugs coupled with the associated costs in developing new drugs and live vaccines increases the need for the development of novel approaches and alternative control strategies for Coccidiosis. This paper aims to review the current progress in understanding the host immune response to Eimeria and discuss current and potential strategies being developed for Coccidiosis control in poultry.
-
recent advances in immunomodulation and vaccination strategies against Coccidiosis
Avian Diseases, 2005Co-Authors: Rami A Dalloul, Hyun S LillehojAbstract:Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies rely heavily on chemoprophylaxis, which is a tremendous cost to the industry. Existing vaccines consist of live virulent or attenuated Eimeria strains with limited scope of protection against an ever-evolving and widespread pathogen. The continual emergence of drug-resistant strains of Eimeria, coupled with the increasing regulations and bans on the use of anticoccidial drugs in commercial poultry production, urges the need for novel approaches and alternative control strategies. Because of the complexity of the host immunity and the parasite life cycle, a comprehensive understanding of host-parasite interactions and protective immune mechanisms becomes necessary for successful prevention and control practices. Recent progress in functional genomics technology would facilitate the identification and characterization of host genes involved in immune responses as well as parasite genes and proteins that elicit protective host responses. This study reviews recent Coccidiosis research and provides information on host immunity, immunomodulation, and the latest advances in live and recombinant vaccine development against Coccidiosis. Such information will help magnify our understanding of host-parasite biology and mucosal immunology, and we hope it will lead to comprehensive designs of nutritional interventions and vaccination strategies for Coccidiosis.
-
application of biotechnological tools for coccidia vaccine development
Journal of Veterinary Science, 2004Co-Authors: Rami A Dalloul, Hyun S LillehojAbstract:: Coccidiosis is a ubiquitous intestinal protozoan infection of poultry seriously impairing the growth and feed utilization of infected animals. Conventional disease control strategies have relied on prophylactic medication. Due to the continual emergence of drug resistant parasites in the field and increasing incidence of broiler condemnations due to coccidia, novel approaches are urgently needed to reduce economic losses. Understanding the basic biology of host-parasite interactions and protective intestinal immune mechanisms, as well as characterization of host and parasite genes and proteins involved in eliciting protective host responses are crucial for the development of new control strategy. This review will highlight recent developments in Coccidiosis research with special emphasis on the utilization of cutting edge techniques in molecular/cell biology, immunology, and functional genomics in coccidia vaccine development. The information will enhance our understanding of host-parasite biology, mucosal immunology, and host and parasite genomics in the development of a practical and effective control strategy against Eimeria and design of nutritional interventions to maximize growth under the stress caused by vaccination or infection. Furthermore, successful identification of quantitative economic traits associated with disease resistance to Coccidiosis will provide poultry breeders with a novel selection strategy for development of genetically stable, Coccidiosis-resistant chickens, thereby increasing the production efficiency.
Bruno Pereira Berto - One of the best experts on this subject based on the ideXlab platform.
-
coccidiose por isospora curio trachta silva et al 2006 apicomplexa eimeriidae em sporophila angolensis linnaeus 1766 numa criacao comercial relato de caso
Brazilian Journal of Veterinary Medicine, 2015Co-Authors: Lilian Cristina De Sousa Oliveira Batista, Marcelo Dos S. D. Vasconcellos, Bruno Do B. Lopes, Matheus Dos M. Passos, Bruno Pereira BertoAbstract:ABSTRACT. Batista L.C. deS.O., Vasconcellos M. dosS.D., dos Passos M.M., Lopes B. doB. & Berto B.P. Coccidiosis due to Isospora curio (Trachta & Silva et al. 2006) in lesser seed-finches Sporophila angolensis (Linnaeus, 1766) at a commercial breeding facility - Case report. [Coccidiose por Isospora curio (Trachta & Silva et al. 2006) (Apicomplexa: Eimeriidae) em Sporophila angolensis (Linnaeus, 1766) numa criacao comercial - Relato de caso]. Revista Brasileira de Medicina Veterinaria, 37(4):401-405, 2015. Curso de Pos-Graduacao em Ciencias Veterinarias, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, Campus Seropedica, BR 465 Km 7, Seropedica, RJ 23890-000, Brasil. E-mail: liliancsobatista@hotmail.com Monitoring of a lesser seed-finch breeding is of great importance, for even the clinically healthy animals may be infected. When Coccidiosis is diagnosed early, it can often be avoided clinical signs of the disease and the number of deaths in the breeding when associated with hygiene practices in the breeding system. Despite of the low frequency of Coccidiosis in the studied passerines, Isospora curio was the unique species found associated with passerines that died in the commercial breeding facility.
-
Coccidiosis due to Isospora curio (Trachta & Silva et al. 2006) in lesser seed-finches Sporophila angolensis (Linnaeus, 1766) at a commercial breeding facility - Case report
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro, 2015Co-Authors: Lilian C. De S. O. Batista, Marcelo Dos S. D. Vasconcellos, Bruno Do B. Lopes, Matheus Dos M. Passos, Bruno Pereira BertoAbstract:ABSTRACT. Batista L.C. deS.O., Vasconcellos M. dosS.D., dos Passos M.M., Lopes B. doB. & Berto B.P. Coccidiosis due to Isospora curio (Trachta & Silva et al. 2006) in lesser seed-finches Sporophila angolensis (Linnaeus, 1766) at a commercial breeding facility - Case report. [Coccidiose por Isospora curio (Trachta & Silva et al. 2006) (Apicomplexa: Eimeriidae) em Sporophila angolensis (Linnaeus, 1766) numa criação comercial - Relato de caso]. Revista Brasileira de Medicina Veterinária, 37(4):401-405, 2015. Curso de Pós-Graduação em Ciências Veterinárias, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Campus Seropédica, BR 465 Km 7, Seropédica, RJ 23890-000, Brasil. E-mail: liliancsobatista@hotmail.com Monitoring of a lesser seed-finch breeding is of great importance, for even the clinically healthy animals may be infected. When Coccidiosis is diagnosed early, it can often be avoided clinical signs of the disease and the number of deaths in the breeding when associated with hygiene practices in the breeding system. Despite of the low frequency of Coccidiosis in the studied passerines, Isospora curio was the unique species found associated with passerines that died in the commercial breeding facility
Sunghyen Lee - One of the best experts on this subject based on the ideXlab platform.
-
effects of anticoccidial and antibiotic growth promoter programs on broiler performance and immune status
Research in Veterinary Science, 2012Co-Authors: Kyungwoo Lee, Yeong Ho Hong, Sunghyen Lee, Seung I Jang, Myeong Seon Park, Daniel A Bautista, Donald G Ritter, Wooseog Jeong, Hyeyoung Jeoung, Erik P LillehojAbstract:This study investigated the effects of various Coccidiosis control programs in combination with antibiotic growth promoters (AGPs) on growth performance and host immune responses in broiler chickens. The Coccidiosis programs that were investigated included in ovo Coccidiosis vaccination (CVAC) with Inovocox or in-feed medication with diclazuril as Clinacox (CLIN) or salinomycin (SAL). The AGPs were virginiamycin or bacitracin methylene disalicylate plus roxarsone. As a negative control, chickens were non-vaccinated and fed with non-supplemented diets (NONE). All animals were exposed to used litter from a commercial broiler farm with confirmed contamination by Eimeria parasites to simulate in-field exposure to avian Coccidiosis. Broiler body weights in the CVAC group were greater at 14 and 32 days of age, but not at day 42, compared with the NONE, CLIN, and SAL groups. At day 14, the SAL group showed decreased body weight and reduced ConA-stimulated spleen cell proliferation compared with the CLIN and SAL groups. In contrast, at days 34 and 43, splenocyte proliferation was greater in the CVAC and CLIN groups compared with the NONE and SAL groups. Lymphocyte subpopulations and cytokine mRNA expression levels in the intestine and spleen were also altered by the denoted treatments. Collectively, these results suggest that in ovo Coccidiosis vaccination or coccidiostat drug medication programs in combination with AGPs influences chicken growth and immune status in an Eimeria-contaminated environment.
-
protective effect of hyperimmune egg yolk igy antibodies against eimeria tenella and eimeria maxima infections
Veterinary Parasitology, 2009Co-Authors: Sunghyen Lee, Hyun S Lillehoj, Seung I Jang, D W Park, A Morales, D Garcia, E Lucio, R Larios, G Victoria, D MarrufoAbstract:Avian Coccidiosis is caused by several distinct species of Eimeria protozoa and is the major parasitic disease of poultry of economic importance. As an alternative strategy to control avian Coccidiosis without using prophylactic medications, we have investigated the efficacy of inducing passive immunity against Coccidiosis by orally feeding hyperimmune IgY antibodies. In this study, a commercially available egg yolk powder, Supracox (SC), a purified IgY fraction of egg yolk prepared from hens hyperimmunized with three major species of Eimeria oocysts, were continuously fed to young chicks from hatch. Upon orally infecting these broiler chicks with Eimeria tenella and Eimeria maxima oocysts at 1 week of age, they showed significantly higher body weight gains (P<0.05) compared to the untreated controls. Furthermore, SC-fed chicks showed significantly less intestinal lesions and reduced fecal oocyst output compared to the untreated controls following oral infections with E. tenella and E. maxima. These results provide clear evidence that passive immunization of chicks with hyperimmune egg yolk IgY antibodies provide significant protection against E. tenella or E. maxima infections.
D Marrufo - One of the best experts on this subject based on the ideXlab platform.
-
protective effect of hyperimmune egg yolk igy antibodies against eimeria tenella and eimeria maxima infections
Veterinary Parasitology, 2009Co-Authors: Sunghyen Lee, Hyun S Lillehoj, Seung I Jang, D W Park, A Morales, D Garcia, E Lucio, R Larios, G Victoria, D MarrufoAbstract:Avian Coccidiosis is caused by several distinct species of Eimeria protozoa and is the major parasitic disease of poultry of economic importance. As an alternative strategy to control avian Coccidiosis without using prophylactic medications, we have investigated the efficacy of inducing passive immunity against Coccidiosis by orally feeding hyperimmune IgY antibodies. In this study, a commercially available egg yolk powder, Supracox (SC), a purified IgY fraction of egg yolk prepared from hens hyperimmunized with three major species of Eimeria oocysts, were continuously fed to young chicks from hatch. Upon orally infecting these broiler chicks with Eimeria tenella and Eimeria maxima oocysts at 1 week of age, they showed significantly higher body weight gains (P<0.05) compared to the untreated controls. Furthermore, SC-fed chicks showed significantly less intestinal lesions and reduced fecal oocyst output compared to the untreated controls following oral infections with E. tenella and E. maxima. These results provide clear evidence that passive immunization of chicks with hyperimmune egg yolk IgY antibodies provide significant protection against E. tenella or E. maxima infections.
Seung I Jang - One of the best experts on this subject based on the ideXlab platform.
-
effects of anticoccidial and antibiotic growth promoter programs on broiler performance and immune status
Research in Veterinary Science, 2012Co-Authors: Kyungwoo Lee, Yeong Ho Hong, Sunghyen Lee, Seung I Jang, Myeong Seon Park, Daniel A Bautista, Donald G Ritter, Wooseog Jeong, Hyeyoung Jeoung, Erik P LillehojAbstract:This study investigated the effects of various Coccidiosis control programs in combination with antibiotic growth promoters (AGPs) on growth performance and host immune responses in broiler chickens. The Coccidiosis programs that were investigated included in ovo Coccidiosis vaccination (CVAC) with Inovocox or in-feed medication with diclazuril as Clinacox (CLIN) or salinomycin (SAL). The AGPs were virginiamycin or bacitracin methylene disalicylate plus roxarsone. As a negative control, chickens were non-vaccinated and fed with non-supplemented diets (NONE). All animals were exposed to used litter from a commercial broiler farm with confirmed contamination by Eimeria parasites to simulate in-field exposure to avian Coccidiosis. Broiler body weights in the CVAC group were greater at 14 and 32 days of age, but not at day 42, compared with the NONE, CLIN, and SAL groups. At day 14, the SAL group showed decreased body weight and reduced ConA-stimulated spleen cell proliferation compared with the CLIN and SAL groups. In contrast, at days 34 and 43, splenocyte proliferation was greater in the CVAC and CLIN groups compared with the NONE and SAL groups. Lymphocyte subpopulations and cytokine mRNA expression levels in the intestine and spleen were also altered by the denoted treatments. Collectively, these results suggest that in ovo Coccidiosis vaccination or coccidiostat drug medication programs in combination with AGPs influences chicken growth and immune status in an Eimeria-contaminated environment.
-
protective effect of hyperimmune egg yolk igy antibodies against eimeria tenella and eimeria maxima infections
Veterinary Parasitology, 2009Co-Authors: Sunghyen Lee, Hyun S Lillehoj, Seung I Jang, D W Park, A Morales, D Garcia, E Lucio, R Larios, G Victoria, D MarrufoAbstract:Avian Coccidiosis is caused by several distinct species of Eimeria protozoa and is the major parasitic disease of poultry of economic importance. As an alternative strategy to control avian Coccidiosis without using prophylactic medications, we have investigated the efficacy of inducing passive immunity against Coccidiosis by orally feeding hyperimmune IgY antibodies. In this study, a commercially available egg yolk powder, Supracox (SC), a purified IgY fraction of egg yolk prepared from hens hyperimmunized with three major species of Eimeria oocysts, were continuously fed to young chicks from hatch. Upon orally infecting these broiler chicks with Eimeria tenella and Eimeria maxima oocysts at 1 week of age, they showed significantly higher body weight gains (P<0.05) compared to the untreated controls. Furthermore, SC-fed chicks showed significantly less intestinal lesions and reduced fecal oocyst output compared to the untreated controls following oral infections with E. tenella and E. maxima. These results provide clear evidence that passive immunization of chicks with hyperimmune egg yolk IgY antibodies provide significant protection against E. tenella or E. maxima infections.