The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform

V N Meyerswallen - One of the best experts on this subject based on the ideXlab platform.

  • exclusion of lhx9 as a candidate gene for sry negative xx sex reversal in the american Cocker Spaniel model
    Journal of Heredity, 2005
    Co-Authors: S Pujar, Kumar S D Kothapalli, E Kirkness, R Van Wormer, V N Meyerswallen
    Abstract:

    : XX sex reversal is known in 17 breeds of dogs. In the American Cocker Spaniel, it segregates as an autosomal recessive trait, and the affected animals lack the testis determining Sry gene. In the search for an autosomal gene that causes this trait, we considered the possibility of Lhx9, a gene encoding LIM homeobox containing transcription factor 9, as a candidate gene. An American Cocker Spaniel pedigree showing Sry-negative XX sex reversal phenotype was genotyped with an intronic Lhx9 microsatellite marker. Segregation of the Lhx9 marker in the pedigree indicated that a mutation in canine Lhx9 is not likely to be the cause of Sry-negative XX sex reversal. In addition, using the recently available 7.6X canine genomic sequence, we report the location and genomic organization of canine Lhx9.

  • sry negative xx sex reversal in the american Cocker Spaniel dog
    Molecular Reproduction and Development, 1995
    Co-Authors: V N Meyerswallen, V Palmer, Gregory M Acland, B Hershfield
    Abstract:

    The Sry gene product serves an important function in male sex determination through testis induction. However, testicular development has been reported in SRY-negative XX sex reversed humans. XX sex reversal of the American Cocker Spaniel, inherited as an autosomal recessive trait, may be a homolog of this disorder. The purpose of this study was to determine whether the Sry high mobility group (HMG) box is present in genomic DNA of affected dogs. Conserved Sry HMG box and hypoxanthine phosphoribosyltransferase (HPRT) sequences were used as primers in polymerase chain reactions. A 167 bp Y-specific canine Sry HMG box sequence was cloned from genomic DNA of normal male dogs. Internal primers generated a 104 bp Sry HMG box product from normal males, but not from females or XX sex reversed dogs. Parallel reactions generated an HPRT product from all dogs. Results indicate that the Sry HMG box is absent in genomic DNA of XX sex reversed dogs. We speculate that activation of the testis differentiation cascade in the absence of Sry in this model is due to a mutant autosomal gene. © 1995 Wiley-Liss, Inc.

B Hershfield - One of the best experts on this subject based on the ideXlab platform.

  • sry negative xx sex reversal in the american Cocker Spaniel dog
    Molecular Reproduction and Development, 1995
    Co-Authors: V N Meyerswallen, V Palmer, Gregory M Acland, B Hershfield
    Abstract:

    The Sry gene product serves an important function in male sex determination through testis induction. However, testicular development has been reported in SRY-negative XX sex reversed humans. XX sex reversal of the American Cocker Spaniel, inherited as an autosomal recessive trait, may be a homolog of this disorder. The purpose of this study was to determine whether the Sry high mobility group (HMG) box is present in genomic DNA of affected dogs. Conserved Sry HMG box and hypoxanthine phosphoribosyltransferase (HPRT) sequences were used as primers in polymerase chain reactions. A 167 bp Y-specific canine Sry HMG box sequence was cloned from genomic DNA of normal male dogs. Internal primers generated a 104 bp Sry HMG box product from normal males, but not from females or XX sex reversed dogs. Parallel reactions generated an HPRT product from all dogs. Results indicate that the Sry HMG box is absent in genomic DNA of XX sex reversed dogs. We speculate that activation of the testis differentiation cascade in the absence of Sry in this model is due to a mutant autosomal gene. © 1995 Wiley-Liss, Inc.

S Pujar - One of the best experts on this subject based on the ideXlab platform.

  • exclusion of lhx9 as a candidate gene for sry negative xx sex reversal in the american Cocker Spaniel model
    Journal of Heredity, 2005
    Co-Authors: S Pujar, Kumar S D Kothapalli, E Kirkness, R Van Wormer, V N Meyerswallen
    Abstract:

    : XX sex reversal is known in 17 breeds of dogs. In the American Cocker Spaniel, it segregates as an autosomal recessive trait, and the affected animals lack the testis determining Sry gene. In the search for an autosomal gene that causes this trait, we considered the possibility of Lhx9, a gene encoding LIM homeobox containing transcription factor 9, as a candidate gene. An American Cocker Spaniel pedigree showing Sry-negative XX sex reversal phenotype was genotyped with an intronic Lhx9 microsatellite marker. Segregation of the Lhx9 marker in the pedigree indicated that a mutation in canine Lhx9 is not likely to be the cause of Sry-negative XX sex reversal. In addition, using the recently available 7.6X canine genomic sequence, we report the location and genomic organization of canine Lhx9.

Hakyoung Yoon - One of the best experts on this subject based on the ideXlab platform.

  • urogenital anomalies and urinary incontinence in an english Cocker Spaniel dog with xx sex reversal
    Journal of Veterinary Internal Medicine, 2018
    Co-Authors: Hakyoung Yoon
    Abstract:

    : A 3-year-old dog weighing 8 kg was referred with a disorder of sexual development and persistent urinary incontinence before and after gonadohysterectomy performed at a local animal hospital. Histopathological examination disclosed hypoplasia of the testes, epididymis, pampiniform plexus, and uterus. On ultrasonography, an anomalous structure containing anechoic fluid was identified in the region dorsal to the urinary bladder. An anomalous communication between the proximal urethra and the remnant uterus and vagina was found on retrograde urethrography under fluoroscopy. Reflux of contrast medium into the anomalous structure, suspected to be the uterus and cranial vagina, from the urethra was detected. Computed tomography identified the anomalous structure between the rectum and urethra. The anomalous structure was removed via laparotomy and the urinary incontinence resolved. The diagnosis of XX sex reversal with a developmental anomaly of the genitourinary tract was made on the basis of laparotomy findings and cytogenetic and SRY gene analyses.

Gregory M Acland - One of the best experts on this subject based on the ideXlab platform.

  • sry negative xx sex reversal in the american Cocker Spaniel dog
    Molecular Reproduction and Development, 1995
    Co-Authors: V N Meyerswallen, V Palmer, Gregory M Acland, B Hershfield
    Abstract:

    The Sry gene product serves an important function in male sex determination through testis induction. However, testicular development has been reported in SRY-negative XX sex reversed humans. XX sex reversal of the American Cocker Spaniel, inherited as an autosomal recessive trait, may be a homolog of this disorder. The purpose of this study was to determine whether the Sry high mobility group (HMG) box is present in genomic DNA of affected dogs. Conserved Sry HMG box and hypoxanthine phosphoribosyltransferase (HPRT) sequences were used as primers in polymerase chain reactions. A 167 bp Y-specific canine Sry HMG box sequence was cloned from genomic DNA of normal male dogs. Internal primers generated a 104 bp Sry HMG box product from normal males, but not from females or XX sex reversed dogs. Parallel reactions generated an HPRT product from all dogs. Results indicate that the Sry HMG box is absent in genomic DNA of XX sex reversed dogs. We speculate that activation of the testis differentiation cascade in the absence of Sry in this model is due to a mutant autosomal gene. © 1995 Wiley-Liss, Inc.