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Zuzana Kovanicova - One of the best experts on this subject based on the ideXlab platform.

  • Metabolomic Analysis Reveals Changes in Plasma Metabolites in Response to Acute Cold Stress and Their Relationships to Metabolic Health in Cold-Acclimatized Humans
    'MDPI AG', 2021
    Co-Authors: Zuzana Kovanicova, Timea Kurdiova, Miroslav Balaž, Miloslav Karhánek, Christian Wolfrum, Barbara Ukropcová, Jozef Ukropec
    Abstract:

    Cold exposure results in activation of metabolic processes required for fueling thermogenesis, potentially promoting improved metabolic health. However, the metabolic complexity underlying this process is not completely understood. We aimed to analyze changes in plasma metabolites related to acute Cold exposure and their relationship to Cold-Acclimatization level and metabolic health in Cold-acclimatized humans. Blood samples were obtained before and acutely after 10–15 min of ice-water swimming (

  • Cold exposure distinctively modulates parathyroid and thyroid hormones in Cold acclimatized and non acclimatized humans
    Endocrinology, 2020
    Co-Authors: Zuzana Kovanicova, Timea Kurdiova, Miroslav Balaž, Patrik Stefanicka, Lukas Varga, Oana C Kulterer, Matthias J Betz
    Abstract:

    Cold-induced activation of thermogenesis modulates energy metabolism, but the role of humoral mediators is not completely understood. We aimed to investigate the role of parathyroid and thyroid hormones in acute and adaptive response to Cold in humans. Examinations were performed before/after 15 minutes of ice-water swimming (n = 15) or 120 to 150 minutes of Cold-induced nonshivering thermogenesis (NST) applied to Cold-acclimatized (n = 6) or non-acclimatized (n = 11) individuals. Deep-neck brown adipose tissue (BAT) was collected from non-acclimatized patients undergoing elective neck surgery (n = 36). Seasonal variations in metabolic/hormonal parameters of ice-water swimmers were evaluated. We found that in ice-water swimmers, PTH and TSH increased and free T3, T4 decreased after a 15-minute winter swim, whereas NST-inducing Cold exposure failed to regulate PTH and free T4 and lowered TSH and free T3. Ice-water swimming-induced increase in PTH correlated negatively with systemic calcium and positively with phosphorus. In non-acclimatized men, NST-inducing Cold decreased PTH and TSH. Positive correlation between systemic levels of PTH and whole-body metabolic preference for lipids as well as BAT volume was found across the 2 populations. Moreover, NST-cooling protocol-induced changes in metabolic preference for lipids correlated positively with changes in PTH. Finally, variability in circulating PTH correlated positively with UCP1/UCP1, PPARGC1A, and DIO2 in BAT from neck surgery patients. Our data suggest that regulation of PTH and thyroid hormones during Cold exposure in humans varies by Cold Acclimatization level and/or Cold stimulus intensity. Possible role of PTH in NST is indicated by its positive relationships with whole-body metabolic preference for lipids, BAT volume, and UCP1 content.

  • Cold exposure distinctively modulates parathyroid and thyroid hormones in Cold acclimatized and non acclimatized humans
    bioRxiv, 2020
    Co-Authors: Zuzana Kovanicova, Timea Kurdiova, Miroslav Balaž, Patrik Stefanicka, Lukas Varga, Oana C Kulterer, Matthias J Betz
    Abstract:

    Abstract Context Cold-induced activation of thermogenesis modulates energy metabolism, but the role of humoral mediators is not completely understood. Objective To investigate the role of parathyroid and thyroid hormones in acute and adaptive response to Cold in humans. Design Cross-sectional study examining acute response to ice-water swimming and to experimental non-shivering thermogenesis (NST) induction in individuals acclimatized and non-acclimatized to Cold. Seasonal variation in energy metabolism of ice-water swimmers and associations between circulating PTH and molecular components of thermogenic program in brown adipose tissue (BAT) of neck-surgery patients were evaluated. Setting Clinical Research Center. Patients, Participants Ice-water swimmers (winter swim n=15, NST-induction n=6), non-acclimatized volunteers (NST-induction, n=11, elective neck surgery n = 36). Main Outcomes and Results In ice-water swimmers, PTH and TSH increased in response to 15min winter swim, while activation of NST failed to regulate PTH and lowered TSH. In non-acclimatized men, NST-induction decreased PTH and TSH. Positive correlation between systemic levels of PTH and whole-body metabolic preference for lipids as well as BAT 18F-FDG uptake was found across the two populations. Moreover, NST-cooling protocol-induced changes in metabolic preference for lipids correlated positively with changes in PTH. Finally, variability in circulating PTH correlated positively with UCP1/UCP1, PPARGC1A and DIO2 in BAT from neck surgery patients. Conclusions Regulation of PTH and thyroid hormones during Cold exposure in humans depends on the Cold Acclimatization level and/or Cold stimulus intensity. Role of PTH in NST is substantiated by its positive relationships with whole-body metabolic preference for lipids, BAT volume and UCP1 content.

Saverio Cinti - One of the best experts on this subject based on the ideXlab platform.

Moharned A. Marahiel - One of the best experts on this subject based on the ideXlab platform.

  • Bacillus subtilis Cold Shock Response 203 Cold Shock Response in Bacillus subtilis
    2013
    Co-Authors: Peter L. Graumann, Moharned A. Marahiel, Biochemie Fachbereich Chemie
    Abstract:

    Dedicated to Rudolf K. Thauer in celebration of his 60 th birthday Following a rapid decrease in temperature, the physiology of Bacillus subtilis cells changes profoundly. Cold shock adaptation has been monitored at the level of membrane composition, adjustment in DNA topology, and change in cytosolic protein synthesis/composition. Some major players in these processes (Cold-stress induced proteins and Cold Acclimatization proteins, CIPs and CAPs) have been identified and mechanisms in Cold shock Acclimatization begin to emerge; however, important questions regarding their cellular function still need to be answered

  • Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis
    Journal of bacteriology, 2002
    Co-Authors: Carsten L. Beckering, Leif Steil, Michael H. W. Weber, Uwe Völker, Moharned A. Marahiel
    Abstract:

    Previous studies with two-dimensional gel electrophoresis techniques revealed that the Cold shock response in Bacillus subtilis is characterized by rapid induction and accumulation of two classes of specific proteins, which have been termed Cold-induced proteins (CIPs) and Cold Acclimatization proteins (CAPs), respectively. Only recently, the B. subtilis two-component system encoded by the desKR operon has been demonstrated to be essential for the Cold-induced expression of the lipid-modifying desaturase Des, which is required for efficient Cold adaptation of the membrane in the absence of isoleucine. At present, one of the most intriguing questions in this research field is whether DesKR plays a global role in Cold signal perception and transduction in B. subtilis. In this report, we present the first genomewide transcriptional analysis of a Cold-exposed bacterium and demonstrate that the B. subtilis two-component system DesKR exclusively controls the desaturase gene des and is not the Cold-triggered regulatory system of global relevance. In addition to this, we identified a set of genes that might participate as novel players in the Cold shock adaptation of B. subtilis. Two Cold-induced genes, the elongation factor homolog ylaG and the σL-dependent transcriptional activator homolog yplP, have been examined by construction and analysis of deletion mutants.

  • Cold shock response in Bacillus subtilis.
    Journal of molecular microbiology and biotechnology, 1999
    Co-Authors: Peter L. Graumann, Moharned A. Marahiel
    Abstract:

    Following a rapid decrease in temperature, the physiology of Bacillus subtilis cells changes profoundly. Cold shock adaptation has been monitored at the level of membrane composition, adjustment in DNA topology, and change in cytosolic protein synthesis/composition. Some major players in these processes (Cold-stress induced proteins and Cold Acclimatization proteins, CIPs and CAPs) have been identified and mechanisms in Cold shock Acclimatization begin to emerge; however, important questions regarding their cellular function still need to be answered.

Miroslav Balaž - One of the best experts on this subject based on the ideXlab platform.

  • Metabolomic Analysis Reveals Changes in Plasma Metabolites in Response to Acute Cold Stress and Their Relationships to Metabolic Health in Cold-Acclimatized Humans
    'MDPI AG', 2021
    Co-Authors: Zuzana Kovanicova, Timea Kurdiova, Miroslav Balaž, Miloslav Karhánek, Christian Wolfrum, Barbara Ukropcová, Jozef Ukropec
    Abstract:

    Cold exposure results in activation of metabolic processes required for fueling thermogenesis, potentially promoting improved metabolic health. However, the metabolic complexity underlying this process is not completely understood. We aimed to analyze changes in plasma metabolites related to acute Cold exposure and their relationship to Cold-Acclimatization level and metabolic health in Cold-acclimatized humans. Blood samples were obtained before and acutely after 10–15 min of ice-water swimming (

  • Cold exposure distinctively modulates parathyroid and thyroid hormones in Cold acclimatized and non acclimatized humans
    Endocrinology, 2020
    Co-Authors: Zuzana Kovanicova, Timea Kurdiova, Miroslav Balaž, Patrik Stefanicka, Lukas Varga, Oana C Kulterer, Matthias J Betz
    Abstract:

    Cold-induced activation of thermogenesis modulates energy metabolism, but the role of humoral mediators is not completely understood. We aimed to investigate the role of parathyroid and thyroid hormones in acute and adaptive response to Cold in humans. Examinations were performed before/after 15 minutes of ice-water swimming (n = 15) or 120 to 150 minutes of Cold-induced nonshivering thermogenesis (NST) applied to Cold-acclimatized (n = 6) or non-acclimatized (n = 11) individuals. Deep-neck brown adipose tissue (BAT) was collected from non-acclimatized patients undergoing elective neck surgery (n = 36). Seasonal variations in metabolic/hormonal parameters of ice-water swimmers were evaluated. We found that in ice-water swimmers, PTH and TSH increased and free T3, T4 decreased after a 15-minute winter swim, whereas NST-inducing Cold exposure failed to regulate PTH and free T4 and lowered TSH and free T3. Ice-water swimming-induced increase in PTH correlated negatively with systemic calcium and positively with phosphorus. In non-acclimatized men, NST-inducing Cold decreased PTH and TSH. Positive correlation between systemic levels of PTH and whole-body metabolic preference for lipids as well as BAT volume was found across the 2 populations. Moreover, NST-cooling protocol-induced changes in metabolic preference for lipids correlated positively with changes in PTH. Finally, variability in circulating PTH correlated positively with UCP1/UCP1, PPARGC1A, and DIO2 in BAT from neck surgery patients. Our data suggest that regulation of PTH and thyroid hormones during Cold exposure in humans varies by Cold Acclimatization level and/or Cold stimulus intensity. Possible role of PTH in NST is indicated by its positive relationships with whole-body metabolic preference for lipids, BAT volume, and UCP1 content.

  • Cold exposure distinctively modulates parathyroid and thyroid hormones in Cold acclimatized and non acclimatized humans
    bioRxiv, 2020
    Co-Authors: Zuzana Kovanicova, Timea Kurdiova, Miroslav Balaž, Patrik Stefanicka, Lukas Varga, Oana C Kulterer, Matthias J Betz
    Abstract:

    Abstract Context Cold-induced activation of thermogenesis modulates energy metabolism, but the role of humoral mediators is not completely understood. Objective To investigate the role of parathyroid and thyroid hormones in acute and adaptive response to Cold in humans. Design Cross-sectional study examining acute response to ice-water swimming and to experimental non-shivering thermogenesis (NST) induction in individuals acclimatized and non-acclimatized to Cold. Seasonal variation in energy metabolism of ice-water swimmers and associations between circulating PTH and molecular components of thermogenic program in brown adipose tissue (BAT) of neck-surgery patients were evaluated. Setting Clinical Research Center. Patients, Participants Ice-water swimmers (winter swim n=15, NST-induction n=6), non-acclimatized volunteers (NST-induction, n=11, elective neck surgery n = 36). Main Outcomes and Results In ice-water swimmers, PTH and TSH increased in response to 15min winter swim, while activation of NST failed to regulate PTH and lowered TSH. In non-acclimatized men, NST-induction decreased PTH and TSH. Positive correlation between systemic levels of PTH and whole-body metabolic preference for lipids as well as BAT 18F-FDG uptake was found across the two populations. Moreover, NST-cooling protocol-induced changes in metabolic preference for lipids correlated positively with changes in PTH. Finally, variability in circulating PTH correlated positively with UCP1/UCP1, PPARGC1A and DIO2 in BAT from neck surgery patients. Conclusions Regulation of PTH and thyroid hormones during Cold exposure in humans depends on the Cold Acclimatization level and/or Cold stimulus intensity. Role of PTH in NST is substantiated by its positive relationships with whole-body metabolic preference for lipids, BAT volume and UCP1 content.

Giorgio Barbatelli - One of the best experts on this subject based on the ideXlab platform.