The Experts below are selected from a list of 138 Experts worldwide ranked by ideXlab platform

Gao Shuang - One of the best experts on this subject based on the ideXlab platform.

Li Bing - One of the best experts on this subject based on the ideXlab platform.

Nathan J. Hillson - One of the best experts on this subject based on the ideXlab platform.

  • Cloud-enabled microscopy and droplet microfluidic platform for specific detection of Escherichia coli
    2016
    Co-Authors: Er Golberg, Gregory Linshiz, Ilia Kravets, Nina Stawski, Nathan J. Hillson, Martin L. Yarmush, Robert S. Marks, Tania Konry
    Abstract:

    We report an all-in-one platform – ScanDrop – for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging system, and cloud-based control software and data storage. The cloud-based control software and data storage enables robotic image acquisition, remote image processing, and rapid data sharing. These features form a ‘‘cloud’ ’ network for water quality monitoring. We have demonstrated the capability of ScanDrop to perform water quality monitoring via the detection of an indicator Coliform Bacterium, Escherichia coli, in drinking water contaminated with feces. Magnetic beads conjugated with antibodies to E. coli antigen were used to selectively capture and isolate specific bacteria from water samples. The bead-captured bacteria were co-encapsulated in pico-liter droplets with fluorescently-labeled anti-E. coli antibodies, and imaged with an automated custom designed fluorescence microscope. The entire water quality diagnostic process required 8 hours from sampl

  • cloud enabled microscopy and droplet microfluidic platform for specific detection of escherichia coli in water
    PLOS ONE, 2014
    Co-Authors: Alexander Golberg, Gregory Linshiz, Ilia Kravets, Nina Stawski, Nathan J. Hillson
    Abstract:

    We report an all-in-one platform – ScanDrop – for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging system, and cloud-based control software and data storage. The cloud-based control software and data storage enables robotic image acquisition, remote image processing, and rapid data sharing. These features form a “cloud” network for water quality monitoring. We have demonstrated the capability of ScanDrop to perform water quality monitoring via the detection of an indicator Coliform Bacterium, Escherichia coli, in drinking water contaminated with feces. Magnetic beads conjugated with antibodies to E. coli antigen were used to selectively capture and isolate specific bacteria from water samples. The bead-captured bacteria were co-encapsulated in pico-liter droplets with fluorescently-labeled anti-E. coli antibodies, and imaged with an automated custom designed fluorescence microscope. The entire water quality diagnostic process required 8 hours from sample collection to online-accessible results compared with 2–4 days for other currently available standard detection methods.

Zhu Bing - One of the best experts on this subject based on the ideXlab platform.

  • Packaging, Amplification, and Appraisal of the Recombinant Tumor-Selective Type I Herpes Simplex Virus Carrying GALV.fus Gene
    Cell biochemistry and biophysics, 2014
    Co-Authors: Zhu Bing, Yang Jian-ru, Jiang Yue-quan, Chen Shifeng, Fu Xinping
    Abstract:

    The aim of the study was to successfully construct three plasmids, which include the GALV.fus gene plasmid regulated by the herpes simplex virus type 1 (HSV-1) late expression gene-UL38 promoter and induced by HSV-1 (HSV-UL38P-GALV.fus), the cytomegalovirus promoter without tumor specificity (CMVP) GALV.fus plasmid (HSV-CMVP-GALV.fus), and the control plasmid in which the GALV.fus gene fragment was replaced by the enhanced green fluorescent protein (EGFP) gene fragment (HSV-CMVP-EGFP). The three constructed plasmids were all packaged and named as Synco-2, Synco-1, and Baco-1. The plasmids were amplified in Coliform Bacterium and transfected into Vero cells using lipofectamine. These recombinant HSV-1 were amplified in Vero cells and purified by conventional methods of cesium chloride, TCID50 method is used to measure virus titers. The total RNA was then extracted from the HepG2 cells transfected by Synco-1 and Synco-2, and the expression of GALV.fus mRNA was detected by RT-PCR. The three recombinant HSV-1 vectors were propagated in Vero cells and purified by cesium chloride density gradient centrifugation, titrated by TCID50 method, and packaged. The titers of Baco-1, Synco-1, and Synco-2 were 3 × 1010, 1 × 1011, and 4 × 1010 pfu/ml. The GALV.fus gene was identified in the infected HepG2 cells by RT-PCR method.

  • Packaging,amplification and identification of recombinant tumor-selective type I herpes simplex virus vector containing GALV.fus gene
    Journal of Chongqing Medical University, 2010
    Co-Authors: Zhu Bing
    Abstract:

    Objective:Package of constructed recombinant tumor-selective type I Herpes simplex virus vector plasmid containing UL38 and CMV promoter and GALV.fus gene (HSV-UL38P-GALV.fus、HSV-CMVP-GALV.fus),GALV.fus gene was replaced by EGFP in control plasmid (HSV-CMVP-EGFP). The three recombinant viruses were named Synco-2、Synco-1 and Baco-1 respectively。Methods: At first the three constructed plasmids were amplificated in Coliform Bacterium and transfected into Vero cells by using lipofectamine respectively. Then,these three recombinant type I Herpes simplex viruses were propagated in Vero cells and purified by cesium chloride density purification,titrated by TCID50 method. In the end,Total RNA was extracted from the HepG2 cells which were transfected by Synco-1 and Synco-2 and GALV.fus mRNA was detected by RT-PCR. Results:The three recombinant HSV-1 vectors were packaged successfully,which were propagated in Vero cells and purified by cesium chloride density purification,titrated by TCID50 method. The titre of Baco-1、Synco-1 and Synco-2 were 3×10 10 pfu/ml,1×10 11 pfu/ml and 4×10 10 pfu/ml respectively.GALV.fus gene were identified in the infected HepG2 cells. Conclusion:We packed,amplificated and purified Recombinant HSV-I virus successfully and the expression of GALV.fus gene was identified in the infected HepG2 cells by RT-PCR.

Timothy J Hughes - One of the best experts on this subject based on the ideXlab platform.

  • An electrochemical investigation of the fouling of a model surface by a Coliform Bacterium
    Biofouling, 1997
    Co-Authors: Rebecca A Illsley, Sharon G Roscoe, Eric D Jackson, Timothy J Hughes
    Abstract:

    An electrochemical investigation of the fouling of metal surfaces by Enterobacter aerogenes was made using the technique of cyclic voltammetry with platinum electrodes. The platinum electrodes were incubated in a tryptic soy broth inoculated with the test organism under conditions of culture age, cell concentration, temperature and time which were predetermined to optimize attachment. Light microscopy and image analysis software was used to verify attachment of the bacteria to the electrode surface. Cyclic voltammetric measurements were made on the electrodes transferred to the electrochemical cell containing phosphate buffer, pH 7.0, at 25°C. The surface charge density resulting from oxidation of the bacterial cell attachments was determined from the difference in the anodic oxidation and reduction areas by computer integration. A decreasing trend in surface charge density of the electrodes during 30 min of potential cycling was partially attributed to the removal of 11 to 38% of the bacterial cells, whi...