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Andrew C. Comrie - One of the best experts on this subject based on the ideXlab platform.
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Modeled response of the West Nile Virus Vector Culex quinquefasciatus to changing climate using the dynamic mosquito simulation model
International Journal of Biometeorology, 2010Co-Authors: Cory W. Morin, Andrew C. ComrieAbstract:Climate can strongly influence the population dynamics of disease Vectors and is consequently a key component of disease ecology. Future climate change and variability may alter the location and seasonality of many disease Vectors, possibly increasing the risk of disease transmission to humans. The mosquito species Culex quinquefasciatus is a concern across the southern United States because of its role as a West Nile Virus Vector and its affinity for urban environments. Using established relationships between atmospheric variables (temperature and precipitation) and mosquito development, we have created the Dynamic Mosquito Simulation Model (DyMSiM) to simulate Cx. quinquefasciatus population dynamics. The model is driven with climate data and validated against mosquito count data from Pasco County, Florida and Coachella Valley, California. Using 1-week and 2-week filters, mosquito trap data are reproduced well by the model ( P
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modeled response of the west nile Virus Vector culex quinquefasciatus to changing climate using the dynamic mosquito simulation model
International Journal of Biometeorology, 2010Co-Authors: Cory W. Morin, Andrew C. ComrieAbstract:Climate can strongly influence the population dynamics of disease Vectors and is consequently a key component of disease ecology. Future climate change and variability may alter the location and seasonality of many disease Vectors, possibly increasing the risk of disease transmission to humans. The mosquito species Culex quinquefasciatus is a concern across the southern United States because of its role as a West Nile Virus Vector and its affinity for urban environments. Using established relationships between atmospheric variables (temperature and precipitation) and mosquito development, we have created the Dynamic Mosquito Simulation Model (DyMSiM) to simulate Cx. quinquefasciatus population dynamics. The model is driven with climate data and validated against mosquito count data from Pasco County, Florida and Coachella Valley, California. Using 1-week and 2-week filters, mosquito trap data are reproduced well by the model (P < 0.0001). Dry environments in southern California produce different mosquito population trends than moist locations in Florida. Florida and California mosquito populations are generally temperature-limited in winter. In California, locations are water-limited through much of the year. Using future climate projection data generated by the National Center for Atmospheric Research CCSM3 general circulation model, we applied temperature and precipitation offsets to the climate data at each location to evaluate mosquito population sensitivity to possible future climate conditions. We found that temperature and precipitation shifts act interdependently to cause remarkable changes in modeled mosquito population dynamics. Impacts include a summer population decline from drying in California due to loss of immature mosquito habitats, and in Florida a decrease in late-season mosquito populations due to drier late summer conditions.
Cory W. Morin - One of the best experts on this subject based on the ideXlab platform.
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Modeled response of the West Nile Virus Vector Culex quinquefasciatus to changing climate using the dynamic mosquito simulation model
International Journal of Biometeorology, 2010Co-Authors: Cory W. Morin, Andrew C. ComrieAbstract:Climate can strongly influence the population dynamics of disease Vectors and is consequently a key component of disease ecology. Future climate change and variability may alter the location and seasonality of many disease Vectors, possibly increasing the risk of disease transmission to humans. The mosquito species Culex quinquefasciatus is a concern across the southern United States because of its role as a West Nile Virus Vector and its affinity for urban environments. Using established relationships between atmospheric variables (temperature and precipitation) and mosquito development, we have created the Dynamic Mosquito Simulation Model (DyMSiM) to simulate Cx. quinquefasciatus population dynamics. The model is driven with climate data and validated against mosquito count data from Pasco County, Florida and Coachella Valley, California. Using 1-week and 2-week filters, mosquito trap data are reproduced well by the model ( P
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modeled response of the west nile Virus Vector culex quinquefasciatus to changing climate using the dynamic mosquito simulation model
International Journal of Biometeorology, 2010Co-Authors: Cory W. Morin, Andrew C. ComrieAbstract:Climate can strongly influence the population dynamics of disease Vectors and is consequently a key component of disease ecology. Future climate change and variability may alter the location and seasonality of many disease Vectors, possibly increasing the risk of disease transmission to humans. The mosquito species Culex quinquefasciatus is a concern across the southern United States because of its role as a West Nile Virus Vector and its affinity for urban environments. Using established relationships between atmospheric variables (temperature and precipitation) and mosquito development, we have created the Dynamic Mosquito Simulation Model (DyMSiM) to simulate Cx. quinquefasciatus population dynamics. The model is driven with climate data and validated against mosquito count data from Pasco County, Florida and Coachella Valley, California. Using 1-week and 2-week filters, mosquito trap data are reproduced well by the model (P < 0.0001). Dry environments in southern California produce different mosquito population trends than moist locations in Florida. Florida and California mosquito populations are generally temperature-limited in winter. In California, locations are water-limited through much of the year. Using future climate projection data generated by the National Center for Atmospheric Research CCSM3 general circulation model, we applied temperature and precipitation offsets to the climate data at each location to evaluate mosquito population sensitivity to possible future climate conditions. We found that temperature and precipitation shifts act interdependently to cause remarkable changes in modeled mosquito population dynamics. Impacts include a summer population decline from drying in California due to loss of immature mosquito habitats, and in Florida a decrease in late-season mosquito populations due to drier late summer conditions.
Aids Control - One of the best experts on this subject based on the ideXlab platform.
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Construction of Vaccinia Virus Vector for Vaccine Screening
Chinese journal of virology, 2004Co-Authors: Zhou Xiao-yun, Liu Ying, Aids ControlAbstract:In order to construct an ideal vaccinia Virus Vector that can facilitate isolation of recombinant Viruses and delete the marker genes more effectively at the process of screening recombinant Virus,vaccinia Virus Vector pSC65 with a single selectable marker was rebuilt to construct vaccinia Virus Vector pVI75 containing neo and lacZ as selectable markers on the basis of transient marker stabilization.To demonstrate the usefulness of Vector pVI75 as an expression Vector,HIV-1 synthetic gene syngpnef was inserted into the special restriction enzyme site to generate transfer plasmid pVI75-syngpnef,then the pVI75-syngpnef co-transfected CEFs with vaccinia Virus Tiantan strain.PCR and Dot blot confirmed the deletion of selectable markers and the insertion of foreign gene HIV-1 syngpnef into the vaccinia Virus genome.Western blot ascertained the expression of target protein in CEFs.All results indicate that vaccinia Virus Vector pVI75 containing two selectable markers facilitates the screening of vaccine and can be used for construction of recombinant vaccinia Virus vaccine.
Rodrigo P. P. Almeida - One of the best experts on this subject based on the ideXlab platform.
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Mealybug transmission of Grapevine leafroll Viruses: an analysis of Virus-Vector specificity.
Phytopathology, 2010Co-Authors: Chi-wei Tsai, Adib Rowhani, Deborah A. Golino, Kent M Daane, Rodrigo P. P. AlmeidaAbstract:Tsai, C.-W., Rowhani, A., Golino, D. A., Daane, K. M., and Almeida, R. P. P. 2010. Mealybug transmission of grapevine leafroll Viruses: An analysis of Virus–Vector specificity. Phytopathology 100:830-834. To understand ecological factors mediating the spread of insect-borne plant pathogens, Vector species for these pathogens need to be identified. Grapevine leafroll disease is caused by a complex of phylogenetically related closteroViruses, some of which are transmitted by insect Vectors; however, the specificities of these complex Virus–Vector interactions are poorly understood thus far. Through biological assays and phylogenetic analyses, we studied the role of Vector-pathogen specificity in the transmission of several grapevine leafroll-associated Viruses (GLRaVs) by their mealybug Vectors. Using plants with multiple Virus infections, several Virus species were screened for Vector transmission by the mealybug species Planococcus ficus and Pseudococcus longispinus. We report that two GLRaVs (-4 and -9), for which no Vector transmission evidence was available, are mealybug-borne. The analyses performed indicated no evidence of mealybug–GLRaV specificity; for example, different Vector species transmitted GLRaV-3 and one Vector species, Planococcus ficus, transmitted five GLRaVs. Based on available data, there is no compelling evidence of Vector–Virus specificity in the mealybug transmission of GLRaVs. However, more studies aimed at increasing the number of mealybug species tested as Vectors of different GLRaVs are necessary. This is especially important given the increasing importance of grapevine leafroll disease spread by mealybugs in vineyards worldwide.
Zhou Xiao-yun - One of the best experts on this subject based on the ideXlab platform.
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Construction of Vaccinia Virus Vector for Vaccine Screening
Chinese journal of virology, 2004Co-Authors: Zhou Xiao-yun, Liu Ying, Aids ControlAbstract:In order to construct an ideal vaccinia Virus Vector that can facilitate isolation of recombinant Viruses and delete the marker genes more effectively at the process of screening recombinant Virus,vaccinia Virus Vector pSC65 with a single selectable marker was rebuilt to construct vaccinia Virus Vector pVI75 containing neo and lacZ as selectable markers on the basis of transient marker stabilization.To demonstrate the usefulness of Vector pVI75 as an expression Vector,HIV-1 synthetic gene syngpnef was inserted into the special restriction enzyme site to generate transfer plasmid pVI75-syngpnef,then the pVI75-syngpnef co-transfected CEFs with vaccinia Virus Tiantan strain.PCR and Dot blot confirmed the deletion of selectable markers and the insertion of foreign gene HIV-1 syngpnef into the vaccinia Virus genome.Western blot ascertained the expression of target protein in CEFs.All results indicate that vaccinia Virus Vector pVI75 containing two selectable markers facilitates the screening of vaccine and can be used for construction of recombinant vaccinia Virus vaccine.