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Julio Galvez - One of the best experts on this subject based on the ideXlab platform.
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differential intestinal anti inflammatory effects of lactobacillus fermentum and lactobacillus salivarius in dss mouse Colitis impact on micrornas expression and microbiota composition
Molecular Nutrition & Food Research, 2017Co-Authors: Alba Rodrigueznogales, Mónica Olivares, Francesca Algieri, Jose Garridomesa, Teresa Vezza, Pilar M Utrilla, Natalia Chueca, Federico Garcia, Elena M Rodriguezcabezas, Julio GalvezAbstract:cope To compare the intestinal anti-inflammatory effects of two probiotics Lactobacillus fermentum and Lactobacillus salivarius in mouse Colitis, focusing on their impact on selected miRNAs and microbiota composition. Methods and results Male C57BL/6J mice were randomly assigned to four groups (n = 10): non-colitic, DSS colitic and two colitic groups treated with probiotics (5 × 108 CFU/mouse/day). Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223. L. fermentum also restored miR-150 and miR-143 expression, also linked to the preservation of the intestinal barrier function. Besides, these beneficial effects were associated with the amelioration of the microbiota dysbiosis and a recovery of the SCFAs- and lactic acid-producing bacterial populations, although only L. fermentum improved Chao richness, Pielou evenness and Shannon diversity. Moreover, L. fermentum also restored the Treg cell population in MLNs and the Th1/Th2 cytokine balance. Conclusion Both probiotics exerted intestinal anti-inflammatory effects in DSS-mouse Colitis, maybe due to their ability to restore the intestinal microbiota homeostasis and modulate the immune response. L. fermentum showed a greater beneficial effect compared to L. salivarius, which makes it more interesting for future studies.
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antiinflammatory and immunomodulatory activity of an ethanolic extract from the stem bark of terminalia catappa l combretaceae in vitro and in vivo evidences
Journal of Ethnopharmacology, 2016Co-Authors: Oyindamola O Abiodun, Alba Rodrigueznogales, Francesca Algieri, Pilar M Utrilla, Elena M Rodriguezcabezas, Ana Maria Gomezcaravaca, Antonio Seguracarretero, Julio GalvezAbstract:Abstract Ethnopharmocological relevance Terminalia catappa Linn (Combretaceae) is a medicinal plant with anti-inflammatory, anti-diarrhoeal and antioxidant properties, frequently found in tropical regions. Considering its characteristics, it could be useful for the treatment of inflammatory bowel disease, which is associated with inflammation, oxidative stress and an immune dysfunction. Thus this study evaluates the immunomodulatory properties and the intestinal anti-inflammatory effect of an ethanolic extract of the stem bark of T. catappa (ETCB) both in vitro (in RAW 264.7 macrophages) and in vivo, in the trinitrobenzenesulfonic acid (TNBS) model of rat Colitis. Materials and methods The phenolic compounds in ETCB were identified and quantified using HPLC-DAD-qTOF-MS. The immunomodulatory activity ETCB was tested in vitro by determining the macrophage production of IL-1β and nitrites. In vivo studies were performed in the TNBS model of rat Colitis. ETCB was given (25, 50 and 100 mg/kg/day) orally for two days prior to Colitis induction and thereafter for 7 days. Response to treatment was assessed by scoring the gross appearance of the colon, and determining myeloperoxidase activity, gene expression of pro-inflammatory cytokines like TNF-α, IL-23 and IL-6, chemokines, inducible nitric oxide synthase and proteins crucial in the maintenance of the intestinal mucosal barrier integrity like mucins (MUC-2, MUC-3) and villin. Results ETCB was able to inhibit IL-1β and nitrite production in vitro in RAW 264.7 macrophages. Moreover, treatment of TNBS colitic rats with ETCB resulted in a decreased colonic damage score and weight/length ratio. It also reduced the colonic neutrophil infiltration indicated by a lower myeloperoxidase activity and prevented the depletion of colonic glutathione levels in colitic rats. In addition, treatment with ETCB down-regulated the gene expression of pro-inflammatory mediators (TNF-α, IL-23, IL-6 and CINC-1) and iNOS in colitic rats. Moreover, the gene expression of mucosal barrier proteins like MUC-2, MUC-3 and villin were up-regulated in colitic rats treated with ETCB. The dose of ETCB that produced the most significant beneficial effect was 100 mg/kg. Regarding the chemical composition of ETCB, 31 phenolic compounds were identified, including ellagic acid, catalagin and gallic acid. Conclusion The beneficial effect of ETCB in the TNBS induced Colitis in rats could be related to its antioxidant, immunomodulatory and anti-inflammatory activities, which could be attributed to the phenolic compounds identified.
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pea pisum sativum l seed albumin extracts show anti inflammatory effect in the dss model of mouse Colitis
Molecular Nutrition & Food Research, 2015Co-Authors: Mᵃ Pilar Utrilla, Julio Galvez, Alba Rodrigueznogales, Francesca Algieri, Mᵃ Jesus Peinado, Raquel Ruiz, Mᵃ Elena Rodriguezcabezas, Alfonso Clemente, Luis A RubioAbstract:cope This study investigates the preventive effects of two pea (Pisum sativum) seed albumin extracts, either in the presence (pea seed extract [PSE]) or absence (albumin fraction from PSE [AF-PSE]) of soluble polysaccharides, in the dextran sodium sulfate (DSS) induced Colitis in mice. Methods and results Male C57BL/6J mice were assigned to five groups: one noncolitic and four colitic. Colitis was induced by incorporating DSS (3.5%) in the drinking water for 4 days, after which DSS was removed. Treated groups received orally PSE (15 g/kg⋅day), or AF-PSE (1.5 g/kg⋅day), or pure soy Bowman–Birk inhibitor (BBI; 50 mg/kg⋅day), starting 2 wk before Colitis induction, and maintained for 9 days after. All treated groups showed intestinal anti-inflammatory effect, evidenced by reduced microscopic histological damage in comparison with untreated colitic mice. The treatments ameliorated the colonic mRNA expression of different proinflammatory markers: cytokines, inducible enzymes, metalloproteinases, adhesion molecules, and toll-like receptors, as well as proteins involved in maintaining the epithelial barrier function. Furthermore, the administration of PSE, AF-PSE, or soy BBI restored bacterial counts, partially or totally, to values in healthy mice. Conclusion PSE and AF-PSE ameliorated DSS-induced damage to mice, their effects being due, at least partially, to the presence of active BBI.
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Effect of kale and papaya supplementation in Colitis induced by trinitrobenzenesulfonic acid in the rat
E-spen The European E-journal of Clinical Nutrition and Metabolism, 2010Co-Authors: Cibele Lima De Albuquerque, Anderson Luiz-ferreira, Alba Regina Monteiro De Souza Brito, Maria Elena Rodriguez-cabezas, Desiree Camuesco, Monica Comalada, Ana Nieto, Antonio Zarzuelo, Julio GalvezAbstract:Summary Background & aims Papaya and kale are usual vegetables in the Brazilian diet that have antioxidant activity. This study proposed to evaluate the effect of dried vegetables as a prebiotic and as an intestinal anti-inflammatory in the rat Colitis model. Methods Rats received, orally, 500 mg/kg of rat weight of three treatments of dried vegetables: papaya, kale and the mixture of both vegetables (60% of kale plus 40% of papaya). In the prebiotic study, after two weeks of treatment, bacteria counts were determined. In the anti-inflammatory study, after the two weeks of treatment, Colitis was induced by intracolonic administration of trinitrobenzenesulfonic acid (TNBS), and one week after, damage score and biochemical parameters were evaluated. Results Only the administration of the mixture was able to modulate the bacterial flora in healthy rats, as well as in rats with Colitis induced by TNBS. In addition, the mixture showed intestinal antiinflammatory effect in the colitic rats. This effect was evidenced by a reduction in damage score, by the colonic iNOS expression downregulated, by the decrease in the production of the TNFα and IL-1β and by the decrease in the MPO activity. Conclusion The combination of both vegetables showed prebiotic and anti-inflammatory effects in the TNBS model of rat Colitis, when compared to each single vegetable alone.
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intestinal antiinflammatory activity of a lyophilized infusion of turnera ulmifolia in tnbs rat Colitis
Fitoterapia, 2006Co-Authors: Julio Galvez, Desiree Camuesco, J S Gracioso, Wagner Vilegas, Alba Regina Monteiro Souza Brito, Antonio ZarzueloAbstract:Turnera ulmifolia is a plant popularly known in Brazil and South America as chanana. Some species of Turnera are widely used in folk medicine for different types of inflammatory diseases. In this study, the preventive intestinal antiinflammatory activity of a lyophilized infusion obtained from the aerial parts of T. ulmifolia was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat Colitis. The results obtained revealed that pretreatment to colitic rats with the extract, at 250 and 500 mg/kg, significantly attenuated the colonic damage induced by TNBS. This beneficial effect was associated with an improvement in the colonic oxidative status, since the infusion prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. On the other hand, this antioxidant activity was confirmed in in vitro studies. In conclusion, the preventive effect exerted by the lyophilized infusion of T. ulmifolia in the TNBS model of rat Colitis is probably related to its antioxidant properties, due to its flavonoids content.
Miquel Sans - One of the best experts on this subject based on the ideXlab platform.
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angiogenesis blockade as a new therapeutic approach to experimental Colitis
Gut, 2007Co-Authors: Silvio Danese, Miquel Sans, David M Spencer, Ivy Beck, Fernando Donate, Marian L Plunkett, Carol A De La Motte, Raymond W Redline, David E Shaw, Alan D LevineAbstract:Neoangiogenesis is a critical component of chronic inflammatory disorders. Inhibition of angiogenesis is an effective therapy in animal models of inflammation, but has not been tested in experimental Colitis. We investigated the effect of ATN-161, an anti-angiogenic compound, on the course of experimental murine Colitis. IL-10-/- mice and wild type (WT) mice were kept in ultra barrier facilities (UBF) or conventional housing, and used for experimental conditions. Dextran sodium sulphate (DSS)-treated mice were used as a model of acute Colitis. Mice were treated with ATN-161 or its scrambled peptide ATN-163. Mucosal neoangiogenesis and mean vascular density (MVD) were assessed by CD31 staining. A disease activity index (DAI) was determined, and severity of Colitis by a blinded histological score. Colonic cytokine production was measured by ELISA, and lamina propria mononuclear cell (LPMC) proliferation by thymidine incorporation. MVD increased in parallel with disease progression in IL-10-/- mice kept in conventional housing, but not in IL-10-/- mice kept in UBF. Angiogenesis also occurred in DSS-treated animals. IL-10-/- mice with established disease treated with ATN-161, but not ATN-163, showed a significant and progressive drop of the DAI. The histological Colitis score was significantly lower in ATN-161-treated compared to scrambled peptide-treated mice. Inhibition of angiogenesis was confirmed by a significant decrease of MVD in ATN-161- compared to ATN-163-treated animals. No therapeutic effects were observed in the DSS model of Colitis. ATN-161 showed no direct immunomodulatory activity in vitro. Active angiogenesis occurs in the gut of IL-10-/- and DSS-treated colitic mice and parallels disease progression. ATN-161 effectively decreases angiogenesis as well as clinical severity and histological inflammation in the IL-10-/- but not the DDS model of IBD. Our results provide the rational basis for considering anti-angiogenic strategies in the treatment of human IBD.
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superoxide dismutase ameliorates tnbs induced Colitis by reducing oxidative stress adhesion molecule expression and leukocyte recruitment into the inflamed intestine
Journal of Leukocyte Biology, 2004Co-Authors: Joaquim Segui, Meritxell Gironella, Miquel Sans, Susana Granell, Felix Gil, Mercedes Gimeno, Pilar Coronel, Josep M Pique, Julian PanesAbstract:Oxidant stress has been implicated in the pathogenesis of inflammatory bowel disease. Antioxidant enzymes, such as superoxide dis- mutase (SOD), are candidate drugs for modulat- ing this pathogenic factor. This study was de- signed to determine the therapeutic value of SOD in an experimental model of Colitis and to study the mechanisms underlying its effects on intestinal inflammation. For that purpose, colitic (trinitrobenzene sulfonic acid-induced) and con- trol rats were studied. Groups of colitic animals were treated with different doses of SOD (1, 4, or 13 mg/kg/day) or vehicle, starting after induc- tion of Colitis and during 7 days. Clinical and pathological markers of Colitis severity and lipid peroxidation in colonic tissue were measured. Leukocyte-endothelial cell interactions in co- lonic venules and expression of vascular cell ad- hesion molecule 1 (VCAM-1) were determined. Development of Colitis was associated with a sig- nificant loss in body weight, an increase in mac- roscopic and microscopic damage scores, and colonic myeloperoxidase activity. Administra- tion of SOD significantly attenuated these changes in a dose-dependent manner and re- duced lipid peroxidation in colonic tissue. The increase in leukocyte rolling and adhesion in co- lonic venules of colitic rats were significantly reduced by administration of SOD, 13 mg/kg/ day. Development of Colitis was associated with a marked increase in endothelial VCAM-1 expres- sion, which was significantly reduced by treat- ment with SOD. In conclusion, treatment with SOD significantly reduces peroxidation reactions in the inflamed colon and affords significant ame- lioration of colonic inflammatory changes in ex- perimental Colitis. This effect is related to a re- duction in VCAM-1 expression and leukocyte re- cruitment into the inflamed intestine. J. Leukoc. Biol. 76: 000 -0 00; 2004.
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vcam 1 and icam 1 mediate leukocyte endothelial cell adhesion in rat experimental Colitis
Gastroenterology, 1999Co-Authors: Miquel Sans, Julian Panes, Esther Ardite, Ignasi J Elizalde, Yolanda Arce, Montserrat Elena, Antonio Palacin, Carlos Fernandez J Checa, Donald C Anderson, Roy R LobbAbstract:Abstract Background & Aims: The molecular mechanisms responsible for leukocyte recruitment in experimental Colitis are poorly understood. The aims of this study were to measure expression of endothelial intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) and to determine their role in leukocyte recruitment in experimental Colitis. Methods: Rats with trinitrobenzene sulfonic acid (TNBS)-induced Colitis and control rats were studied 1, 7, or 21 days after treatment. ICAM-1 and VCAM-1 expressions were measured by the double radiolabeled antibody technique. Leukocyte-endothelial cell interactions were determined in colonic venules by fluorescence intravital microscopy. Therapeutic effects of treatment with anti–VCAM-1 antibodies were also assessed. Results: Colonic endothelial ICAM-1 was constitutively expressed and did not increase in colitic animals. In contrast, constitutive expression of VCAM-1 was low but markedly increased (6-fold) 1 and 7 days after induction of Colitis. Increased colonic expression of VCAM-1 paralleled macroscopic damage score, myeloperoxidase activity, and increased leukocyte adhesion in colonic venules. The latter was significantly decreased by immunoneutralization of ICAM-1 and completely abrogated by immunoneutralization of VCAM-1. Long-term administration of anti–VCAM-1 antibody resulted in significant attenuation of Colitis. Conclusions: Induction of Colitis in rats by TNBS is followed by up-regulation of endothelial VCAM-1. VCAM-1 and constitutive ICAM-1 are major determinants of leukocyte recruitment to the inflamed intestine. GASTROENTEROLOGY 1999;116:874-883
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vcam 1 and icam 1 mediate leukocyte endothelial cell adhesion in rat experimental Colitis
Gastroenterology, 1999Co-Authors: Miquel Sans, Julian Panes, Esther Ardite, Ignasi J Elizalde, Yolanda Arce, Montserrat Elena, Antonio Palacin, Carlos Fernandez J Checa, Donald C Anderson, Roy R LobbAbstract:Abstract Background & Aims: The molecular mechanisms responsible for leukocyte recruitment in experimental Colitis are poorly understood. The aims of this study were to measure expression of endothelial intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) and to determine their role in leukocyte recruitment in experimental Colitis. Methods: Rats with trinitrobenzene sulfonic acid (TNBS)-induced Colitis and control rats were studied 1, 7, or 21 days after treatment. ICAM-1 and VCAM-1 expressions were measured by the double radiolabeled antibody technique. Leukocyte-endothelial cell interactions were determined in colonic venules by fluorescence intravital microscopy. Therapeutic effects of treatment with anti–VCAM-1 antibodies were also assessed. Results: Colonic endothelial ICAM-1 was constitutively expressed and did not increase in colitic animals. In contrast, constitutive expression of VCAM-1 was low but markedly increased (6-fold) 1 and 7 days after induction of Colitis. Increased colonic expression of VCAM-1 paralleled macroscopic damage score, myeloperoxidase activity, and increased leukocyte adhesion in colonic venules. The latter was significantly decreased by immunoneutralization of ICAM-1 and completely abrogated by immunoneutralization of VCAM-1. Long-term administration of anti–VCAM-1 antibody resulted in significant attenuation of Colitis. Conclusions: Induction of Colitis in rats by TNBS is followed by up-regulation of endothelial VCAM-1. VCAM-1 and constitutive ICAM-1 are major determinants of leukocyte recruitment to the inflamed intestine. GASTROENTEROLOGY 1999;116:874-883
Charles O. Elson - One of the best experts on this subject based on the ideXlab platform.
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th17 cells induce Colitis and promote th1 cell responses through il 17 induction of innate il 12 and il 23 production
Journal of Immunology, 2011Co-Authors: Ting Feng, Charles O. Elson, Hongwei Qin, Lanfang Wang, Etty N Benveniste, Yingzi CongAbstract:Both Th1 and Th17 cells have been implicated in the pathogenesis of inflammatory bowel disease and experimental Colitis. However, the complex relationship between Th1 and Th17 cells and their relative contributions to the pathogenesis of inflammatory bowel disease have not been completely analyzed. Although it has been recently shown that Th17 cells can convert into Th1 cells, the underlying in vivo mechanisms and the role of Th1 cells converted from Th17 cells in the pathogenesis of Colitis are still largely unknown. In this study, we report that Th17 cells from CBir1 TCR transgenic mice, which are specific for an immunodominant microbiota Ag, are more potent than Th1 cells in the induction of Colitis, as Th17 cells induced severe Colitis, whereas Th1 cells induced mild Colitis when transferred into TCRβxδ−/− mice. High levels of IL-12 and IL-23 and substantial numbers of IFN-γ+ Th1 cells emerged in the colons of Th17 cell recipients. Administration of anti–IL-17 mAb abrogated Th17 cell-induced Colitis development, blocked colonic IL-12 and IL-23 production, and inhibited IFN-γ+ Th1 cell induction. IL-17 promoted dendritic cell production of IL-12 and IL-23. Furthermore, conditioned media from colonic tissues of colitic Th17 cell recipients induced IFN-γ production by Th17 cells, which was inhibited by blockade of IL-12 and IL-23. Collectively, these data indicate that Th17 cells convert to Th1 cells through IL-17 induction of mucosal innate IL-12 and IL-23 production.
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cd4 t cells reactive to enteric bacterial antigens in spontaneously colitic c3h hejbir mice increased t helper cell type 1 response and ability to transfer disease
Journal of Experimental Medicine, 1998Co-Authors: Yingzi Cong, Steven L Brandwein, Robert P Mccabe, Edward H Birkenmeier, John P Sundberg, Audrey J Lazenby, Charles O. ElsonAbstract:C3H/HeJBir mice are a new substrain that spontaneously develop Colitis early in life. This study was done to determine the T cell reactivity of C3H/HeJBir mice to candidate antigens that might be involved in their disease. C3H/HeJBir CD4+ T cells were strongly reactive to antigens of the enteric bacterial flora, but not to epithelial or food antigens. The stimulatory material in the enteric bacteria was trypsin sensitive and restricted by class II major histocompatibility complex molecules, but did not have the properties of a superantigen. The precursor frequency of interleuken (IL)-2–producing, bacterial-reactive CD4+ T cells in colitic mice was 1 out of 2,000 compared to 1 out of 20,000–25,000 in noncolitic control mice. These T cells produced predominately IL-2 and interferon γ, consistent with a T helper type 1 cell response and were present at 3–4 wk, the age of onset of the Colitis. Adoptive transfer of bacterial-antigen–activated CD4+ T cells from colitic C3H/HeJBir but not from control C3H/HeJ mice into C3H/HeSnJ scid/scid recipients induced Colitis. These data represent a direct demonstration that T cells reactive with conventional antigens of the enteric bacterial flora can mediate chronic inflammatory bowel disease.
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spontaneously colitic c3h hejbir mice demonstrate selective antibody reactivity to antigens of the enteric bacterial flora
Journal of Immunology, 1997Co-Authors: Steven L Brandwein, Yingzi Cong, Robert P Mccabe, Ken B Waites, Ben U Ridwan, Phillip Dean, Toshifumi Ohkusa, Edward H Birkenmeier, John P Sundberg, Charles O. ElsonAbstract:The idiopathic inflammatory bowel diseases, ulcerative Colitis and Crohn's disease, are chronic disorders that appear to arise from an aberrant interaction of environmental, genetic, and immunologic factors. The aim of this study was to examine the immune reactivity of a spontaneously colitic mouse strain, C3H/HeJBir, to epithelial, food, and enteric bacterial Ags. Serum Ab responses of colitic C3H/HeJBir and noncolitic parental C3H/HeJ mice were measured by enhanced chemiluminescence Western blotting. No reactivity to epithelial or food Ags was detected. However, the sera from C3H/HeJBir mice had a reproducible banding pattern on Western blot to bacterial Ags, whereas sera from C3H/HeJ mice did not. Only a small, highly selected number of enteric bacterial Ags were recognized. There were major differences in the degree of recognition of different bacterial strains, marked by remarkably few Abs to Ags of the major anaerobes of the bacterial flora. The serum Abs detected on immunoblot were primarily IgG2a, suggesting a Th1 response. Comparison of sera reactivity to histopathologic severity showed an inverse relationship: one third of young C3H/HeJBir mice during the peak of Colitis produced Abs to bacterial Ags, while later in life, when the Colitis had resolved, 96% produced Abs. These data are consistent with an abnormal immune reactivity to enteric bacterial flora in C3H/HeJBir mice, a reactivity that is highly selective considering the abundant bacterial Ags present in the colon lumen. We postulate that this reactivity plays a role in the pathogenesis of Colitis in these mice.
Roy R Lobb - One of the best experts on this subject based on the ideXlab platform.
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vcam 1 and icam 1 mediate leukocyte endothelial cell adhesion in rat experimental Colitis
Gastroenterology, 1999Co-Authors: Miquel Sans, Julian Panes, Esther Ardite, Ignasi J Elizalde, Yolanda Arce, Montserrat Elena, Antonio Palacin, Carlos Fernandez J Checa, Donald C Anderson, Roy R LobbAbstract:Abstract Background & Aims: The molecular mechanisms responsible for leukocyte recruitment in experimental Colitis are poorly understood. The aims of this study were to measure expression of endothelial intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) and to determine their role in leukocyte recruitment in experimental Colitis. Methods: Rats with trinitrobenzene sulfonic acid (TNBS)-induced Colitis and control rats were studied 1, 7, or 21 days after treatment. ICAM-1 and VCAM-1 expressions were measured by the double radiolabeled antibody technique. Leukocyte-endothelial cell interactions were determined in colonic venules by fluorescence intravital microscopy. Therapeutic effects of treatment with anti–VCAM-1 antibodies were also assessed. Results: Colonic endothelial ICAM-1 was constitutively expressed and did not increase in colitic animals. In contrast, constitutive expression of VCAM-1 was low but markedly increased (6-fold) 1 and 7 days after induction of Colitis. Increased colonic expression of VCAM-1 paralleled macroscopic damage score, myeloperoxidase activity, and increased leukocyte adhesion in colonic venules. The latter was significantly decreased by immunoneutralization of ICAM-1 and completely abrogated by immunoneutralization of VCAM-1. Long-term administration of anti–VCAM-1 antibody resulted in significant attenuation of Colitis. Conclusions: Induction of Colitis in rats by TNBS is followed by up-regulation of endothelial VCAM-1. VCAM-1 and constitutive ICAM-1 are major determinants of leukocyte recruitment to the inflamed intestine. GASTROENTEROLOGY 1999;116:874-883
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vcam 1 and icam 1 mediate leukocyte endothelial cell adhesion in rat experimental Colitis
Gastroenterology, 1999Co-Authors: Miquel Sans, Julian Panes, Esther Ardite, Ignasi J Elizalde, Yolanda Arce, Montserrat Elena, Antonio Palacin, Carlos Fernandez J Checa, Donald C Anderson, Roy R LobbAbstract:Abstract Background & Aims: The molecular mechanisms responsible for leukocyte recruitment in experimental Colitis are poorly understood. The aims of this study were to measure expression of endothelial intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) and to determine their role in leukocyte recruitment in experimental Colitis. Methods: Rats with trinitrobenzene sulfonic acid (TNBS)-induced Colitis and control rats were studied 1, 7, or 21 days after treatment. ICAM-1 and VCAM-1 expressions were measured by the double radiolabeled antibody technique. Leukocyte-endothelial cell interactions were determined in colonic venules by fluorescence intravital microscopy. Therapeutic effects of treatment with anti–VCAM-1 antibodies were also assessed. Results: Colonic endothelial ICAM-1 was constitutively expressed and did not increase in colitic animals. In contrast, constitutive expression of VCAM-1 was low but markedly increased (6-fold) 1 and 7 days after induction of Colitis. Increased colonic expression of VCAM-1 paralleled macroscopic damage score, myeloperoxidase activity, and increased leukocyte adhesion in colonic venules. The latter was significantly decreased by immunoneutralization of ICAM-1 and completely abrogated by immunoneutralization of VCAM-1. Long-term administration of anti–VCAM-1 antibody resulted in significant attenuation of Colitis. Conclusions: Induction of Colitis in rats by TNBS is followed by up-regulation of endothelial VCAM-1. VCAM-1 and constitutive ICAM-1 are major determinants of leukocyte recruitment to the inflamed intestine. GASTROENTEROLOGY 1999;116:874-883
Antonio Zarzuelo - One of the best experts on this subject based on the ideXlab platform.
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Effect of kale and papaya supplementation in Colitis induced by trinitrobenzenesulfonic acid in the rat
E-spen The European E-journal of Clinical Nutrition and Metabolism, 2010Co-Authors: Cibele Lima De Albuquerque, Anderson Luiz-ferreira, Alba Regina Monteiro De Souza Brito, Maria Elena Rodriguez-cabezas, Desiree Camuesco, Monica Comalada, Ana Nieto, Antonio Zarzuelo, Julio GalvezAbstract:Summary Background & aims Papaya and kale are usual vegetables in the Brazilian diet that have antioxidant activity. This study proposed to evaluate the effect of dried vegetables as a prebiotic and as an intestinal anti-inflammatory in the rat Colitis model. Methods Rats received, orally, 500 mg/kg of rat weight of three treatments of dried vegetables: papaya, kale and the mixture of both vegetables (60% of kale plus 40% of papaya). In the prebiotic study, after two weeks of treatment, bacteria counts were determined. In the anti-inflammatory study, after the two weeks of treatment, Colitis was induced by intracolonic administration of trinitrobenzenesulfonic acid (TNBS), and one week after, damage score and biochemical parameters were evaluated. Results Only the administration of the mixture was able to modulate the bacterial flora in healthy rats, as well as in rats with Colitis induced by TNBS. In addition, the mixture showed intestinal antiinflammatory effect in the colitic rats. This effect was evidenced by a reduction in damage score, by the colonic iNOS expression downregulated, by the decrease in the production of the TNFα and IL-1β and by the decrease in the MPO activity. Conclusion The combination of both vegetables showed prebiotic and anti-inflammatory effects in the TNBS model of rat Colitis, when compared to each single vegetable alone.
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a comparative study of the preventative effects exerted by two probiotics lactobacillus reuteri and lactobacillus fermentum in the trinitrobenzenesulfonic acid model of rat Colitis
British Journal of Nutrition, 2007Co-Authors: Laura Peran, Monica Comalada, Ana Nieto, Antonio Zarzuelo, Elvira Bailon, Saleta Sierra, Ángel Concha, Mónica Olivares, Federico Laravilloslada, Jordi XausAbstract:The intestinal anti-inflammatory effects of two probiotics isolated from breast milk, Lactobacillus reuteri and L. fermentum, were evaluated and compared in the trinitrobenzenesulfonic acid (TNBS) model of rat Colitis. Colitis was induced in rats by intracolonic administration of 10 mg TNBS dissolved in 50% ethanol (0.25 ml). Either L. reuteri or L. fermentum was daily administered orally (5 x 10(8) colony-forming units suspended in 0.5 ml skimmed milk) to each group of rats (n 10) for 3 weeks, starting 2 weeks before Colitis induction. Colonic damage was evaluated histologically and biochemically, and the colonic luminal contents were used for bacterial studies and for SCFA production. Both probiotics showed intestinal anti-inflammatory effects in this model of experimental Colitis, as evidenced histologically and by a significant reduction of colonic myeloperoxidase activity (P<0.05). L. fermentum significantly counteracted the colonic glutathione depletion induced by the inflammatory process. In addition, both probiotics lowered colonic TNFalpha levels (P<0.01) and inducible NO synthase expression when compared with non-treated rats; however, the decrease in colonic cyclo-oxygenase-2 expression was only achieved with L.fermentum administration. Finally, the two probiotics induced the growth of Lactobacilli species in comparison with control colitic rats, but the production of SCFA in colonic contents was only increased when L. fermentum was given. In conclusion, L. fermentum can exert beneficial immunomodulatory properties in inflammatory bowel disease, being more effective than L. reuteri, a probiotic with reputed efficacy in promoting beneficial effects on human health.
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intestinal antiinflammatory activity of a lyophilized infusion of turnera ulmifolia in tnbs rat Colitis
Fitoterapia, 2006Co-Authors: Julio Galvez, Desiree Camuesco, J S Gracioso, Wagner Vilegas, Alba Regina Monteiro Souza Brito, Antonio ZarzueloAbstract:Turnera ulmifolia is a plant popularly known in Brazil and South America as chanana. Some species of Turnera are widely used in folk medicine for different types of inflammatory diseases. In this study, the preventive intestinal antiinflammatory activity of a lyophilized infusion obtained from the aerial parts of T. ulmifolia was tested in the trinitrobenzenesulphonic acid (TNBS) model of rat Colitis. The results obtained revealed that pretreatment to colitic rats with the extract, at 250 and 500 mg/kg, significantly attenuated the colonic damage induced by TNBS. This beneficial effect was associated with an improvement in the colonic oxidative status, since the infusion prevented the glutathione depletion that occurred as a consequence of the colonic inflammation. On the other hand, this antioxidant activity was confirmed in in vitro studies. In conclusion, the preventive effect exerted by the lyophilized infusion of T. ulmifolia in the TNBS model of rat Colitis is probably related to its antioxidant properties, due to its flavonoids content.
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goat milk oligosaccharides are anti inflammatory in rats with hapten induced Colitis
Journal of Nutrition, 2006Co-Authors: Abdelali Daddaoua, Victor Puerta, Pilar Requena, A Martinezferez, Emilia M Guadix, Fermin Sanchez De Medina, Antonio Zarzuelo, Maria Dolores Suarez, Julio Boza, Olga MartinezaugustinAbstract:Oligosaccharides are included among the anti-inflammatory components of milk because of their prebiotic properties and their capacity to act as receptors of microorganisms. Here the intestinal anti-inflammatory effect of goat milk oligosaccharides (O) was assessed in trinitrobenzenesulfonic (T) acid-induced Colitis in rats. Rats were randomly assigned to three different groups. Two groups (T and OS) of colitic rats and a control group (C) were studied. Group OS received 500 mg/(kg.d) of goat milk oligosaccharides orally, starting 2 d before the Colitis induction until d 6, and groups T and C received the vehicle. When compared with the T group, the OS group showed decreased anorexia and body weight loss; reduced bowel wall thickening and longitudinal extension of necrotic lesions; downregulated colonic expression of interleukin 1beta, inducible nitric oxide synthase, cyclooxygenase 2, and mucin 3; and increased trefoil factor 3. Thus, goat milk oligosaccharides have anti-inflammatory effects in rats with experimental Colitis and may be useful in the management of inflammatory bowel disease.
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preventative effects of a probiotic lactobacillus salivarius ssp salivarius in the tnbs model of rat Colitis
World Journal of Gastroenterology, 2005Co-Authors: Laura Peran, Desiree Camuesco, Monica Comalada, Ana Nieto, Antonio Zarzuelo, Ángel Concha, Mónica Olivares, Jordi Xaus, Maria Paz Diazropero, Julio GalvezAbstract:AIM: To investigate the intestinal anti-inflammatory effect and mechanism of a probiotic Lactobacillus salivarius ssp. salivarius CECT5713 in the TNBS model of rat Colitis. METHODS: Female Wistar rats (180-200 g) were used in this study. A group of rats were administered orally the probiotic L. salivarius ssp. salivarius (5×108 CFU suspended in 0.5 mL of skimmed milk) daily for 3 wk. Two additional groups were used for reference, a non-colitic and a control colitic without probiotic treatment, which received orally the vehicle used to administer the probiotic. Two weeks after starting the experiment, the rats were rendered colitic by intracolonic administration of 10 mg of TNBS dissolved in 0.25 mL of 500 mL/L ethanol. One week after Colitis induction, all animals were killed and colonic damage was evaluated both histologically and biochemically. The biochemical studies performed in colonic homogenates include determination of myeloperoxidase (MPO) activity, glutathione (GSH) content, leukotriene B4 (LTB4) and tumor necrosis factor α (TNF-α) levels, as well as inducible nitric oxide synthase (iNOS) expression. In addition, the luminal contents obtained from colonic samples were used for microbiological studies, in order to determine Lactobacilli and Bifidobacteria counts. RESULTS: Treatment of colitic rats with L. salivarius ssp. salivarius resulted in amelioration of the inflammatory response in colitic rats, when compared with the corresponding control group without probiotic treatment. This anti-inflammatory effect was evidenced macroscopically by a significant reduction in the extent of colonic necrosis and/or inflammation induced by the administration of TNBS/ethanol (2.3±0.4 cm vs 3.4±0.3 cm in control group, P<0.01) and histologically by improvement of the colonic architecture associated with a reduction in the neutrophil infiltrate in comparison with non-treated colitic rats. The latter was confirmed biochemically by a significant reduction of colonic MPO activity (105.3±26.0 U/g vs 180.6±21.9 U/g, P<0.05), a marker of neutrophil infiltration. The beneficial effect was associated with an increase of the colonic GSH content (1 252±42 nmol/g vs 1 087±51 nmol/g, P<0.05), which is depleted in colitic rats, as a consequence of the oxidative stress induced by the inflammatory process. In addition, the treatment of colitic rats with L. salivarius resulted in a significant reduction of colonic TNF-α levels (509.4±68.2 pg/g vs 782.9±60.1 pg/g, P<0.01) and in a lower colonic iNOS expression, when compared to TNBS control animals without probiotic administration. Finally, treated colitic rats showed higher counts of Lactobacilli species in colonic contents than control colitic rats, whereas no differences were observed in Bifidobacteria counts. CONCLUSION: Administration of the probiotic L. salivarius ssp. salivarius CECT5713 facilitates the recovery of the inflamed tissue in the TNBS model of rat Colitis, an effect associated with amelioration of the production of some of the mediators involved in the inflammatory response in the intestine, such as cytokines, including TNF-α and NO. This beneficial effect could be ascribed to its effect on the altered immune response that occurs in this inflammatory condition.
