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P. Bartoš - One of the best experts on this subject based on the ideXlab platform.
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reaction of wheat alternative wheat and triticale cultivars to Common Bunt
Czech Journal of Genetics and Plant Breeding, 2018Co-Authors: V. Dumalasová, P. BartošAbstract:Seventeen winter wheat cultivars registered in the Czech Republic were tested for reaction to Common Bunt in 2–3 year field trials. Bunt infection of resistant checks Globus and Bill varied between 4.1% and 10.6%; the highest infection in cv. Pitbull reached 85.9%. Of the recently registered cultivars Nikol has a relatively low Bunt incidence (26.9%). In addition to bread wheat seventeen triticale, seven durum wheat cultivars, two spelt wheat cultivars and one emmer wheat cultivar were tested in the field and some of them also in the greenhouse. Bunt infection of durum wheats was lower than that of bread wheat cultivars. All seventeen tested triticale cultivars were resistant. The reaction of emmer wheat cultivar and spelt wheat cultivars to Common Bunt was lower than that of susceptible bread wheat checks.
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Common Bunt resistance of czech and european winter wheat cultivars and breeder lines
Czech Journal of Genetics and Plant Breeding, 2018Co-Authors: Veronika Dumalasova, Leona Leisovasvobodova, P. BartošAbstract:Dumalasova V., Leisova-Svobodova L., Bartos P. (2014): Common Bunt resistance of Czech and European winter wheat cultivars and breeder lines. Czech J. Genet. Plant Breed., 50: 201–207. Winter wheat cultivars recently registered in the Czech Republic were tested in three-year field tests for resistance to Common Bunt. Seeds were inoculated with a mixture of local strains of Tilletia tritici and T. laevis. None of the cultivars displayed a higher level of resistance compared with the resistant checks. The mean percentage of Bunted ears in the three test series including checks was 39%. Mean Bunt infection in resistant and susceptible checks was 2% and 63%, respectively. In the European Tilletia cooperative test performed in Prague-Ruzyně, thirty-five winter wheat cultivars from six countries were tested during 2007–2013. The cultivars Bill, Nadro, Quebon, Samurai, Stava and Tommi exhibited infection levels below 10% in the respective years of the test. Additionally, 75 breeding lines from six countries were tested. Infection levels below 1% were recorded in 56% of the lines and 1–10% levels in 19% of the lines. A close relationship between the resistant cvs. Tommi and Globus was confirmed using SSR allelic markers.
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reaction of spring wheat cultivars to Common Bunt caused by tilletia tritici bjerk wint and tilletia laevis kuhn
Czech Journal of Genetics and Plant Breeding, 2018Co-Authors: Veronika Dumalasova, P. BartošAbstract:In 2005, 2006 and 2007 nineteen, eight and nine spring wheat cultivars, respectively, were tested in field trials for resistance to Common Bunt after inoculation with Bunt teliospores. Nine spring wheat cultivars were tested in a greenhouse under favourable conditions for the Bunt infection. Bunt incidence in the field trials varied between 0% and 38.7%, in the greenhouse between 52.9% and 100%. The results of individual cultivars and years fluctuated. A reduction in plant height, ear length, root system and increased tillering were regis - tered in the inoculated plants. Spots on the leaves of inoculated plants were observed in three out of the four greenhouse experiments.
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reaction of wheat to Common Bunt and dwarf Bunt and reaction of triticale to dwarf Bunt
Czech Journal of Genetics and Plant Breeding, 2016Co-Authors: Veronika Dumalasova, P. BartošAbstract:Resistance to Common Bunt and to dwarf Bunt in winter wheat cultivars recently registered in the Czech Republic was studied in artificially inoculated field trials in the years 2013−2015. In trials with Common Bunt, seeds of each experimental series were inoculated with a different mixture of isolates. In dwarf Bunt trials, the soil surface was inoculated with a natural Bunt population from a single locality. Several selected unregistered wheat cultivars, triticale cultivars and cultivars/lines known as sources of dwarf Bunt resistance were also included in the trials with dwarf Bunt. Out of the recently registered winter wheat cultivars only cv. Genius showed resistance to Common Bunt in both test years. Cv. Sailor was highly resistant to Common Bunt only in one trial, but not in other trials with different inoculum. Cv. Saturnus and the registered cv. Potenzial showed the lowest incidence of dwarf Bunt in both years. The triticale cultivars were highly resistant to dwarf Bunt compared to the wheat cultivars used as checks. High resistance to dwarf Bunt in the tested sources of resistance was confirmed.
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Common Bunt resistance of Czech and European winter wheat cultivars and breeder lines
Czech Journal of Genetics and Plant Breeding, 2014Co-Authors: V. Dumalasová, L. Leišová-svobodová, P. BartošAbstract:Winter wheat cultivars recently registered in the Czech Republic were tested in three-year field tests for resistance to Common Bunt. Seeds were inoculated with a mixture of local strains of Tilletia tritici and T. laevis. None of the cultivars displayed a higher level of resistance compared with the resistant checks. The mean percentage of Bunted ears in the three test series including checks was 39%. Mean Bunt infection in resistant and susceptible checks was 2% and 63%, respectively. In the European Tilletia cooperative test performed in Prague-Ruzyně, thirty-five winter wheat cultivars from six countries were tested during 2007–2013. The cultivars Bill, Nadro, Quebon, Samurai, Stava and Tommi exhibited infection levels below 10% in the respective years of the test. Additionally, 75 breeding lines from six countries were tested. Infection levels below 1% were recorded in 56% of the lines and 1–10% levels in 19% of the lines. A close relationship between the resistant cvs. Tommi and Globus was confirmed using SSR allelic markers.
Anders Borgen - One of the best experts on this subject based on the ideXlab platform.
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Resistance to Common Bunt (Tilletia tritici) and rust (Puccinia sp.) in hulled wheat
2020Co-Authors: Anders BorgenAbstract:Common Bunt (Tilletia tritici) and rust diseases (Puccinia sp.) was scored in 123 varieties of wheat species other than bread wheat. The species included Triticum spelta (90 lines), T.macha (18 lines), T.dicoccon (6 lines), T.timopheevii (3 lines) and one representative of each of the species T.vavilovii, T.karamyshevii, T. polonicum, T.carthlicum and T.compactum. Huge differences in Bunt susceptibility were found in all species. Lines with low susceptibility were identified in Triticum spelta, T.macha, T.dicoccon, T.timopheevii and T.vavilovii but none in the few investigated lines of species T.karamyshevii, T. polonicum and T.carthlicum. All lines of T.macha, T.vavilovii and T.karamyshevii were susceptible to rust, were as resistance to this disease was frequent in T. spelta. The study hereby has identified candidates for future plant breeding within these species. The lines were also screened for their ability to be sown as spring crops, which can be used as a strategy to control Common Bunt. Only few lines of T.spelta were of this intermediate type, whereas half of the lines of T.macha, T.dicoccon, T.timopheevii were.
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managing Common Bunt in wheat seed lots liveseed practice abstract
2019Co-Authors: Anders Borgen, Stephanie Klaedtke, Angela ThueringerAbstract:Seed analyses: A seed analysis, as performed by state-accredited labs for example, will confirm and quantify the infection of a seed lot with Common Bunt. Thorough seed cleaning: Thoroughly cleaning an infected seed lot with an air stream or similar gravity cleaning equipment can remove most of the intact Bunt balls and some of the free spores. As a second step, brush-cleaning is very efficient to reduce the number of free spores in the seed lot. Seed treatments: Seed treatments are essential to prevent and control Common Bunt. Several seed treatments are authorized for organic farming, namely white vinegar, mustard powder, products based on antagonist microorganisms (e.g. Cerall (R)) and products based on copper (e.g. Copseed), depending on the country. When harvesting... If an infection with Common Bunt is suspected, harvest healthy wheat fields first and infected fields last. Then clean the harvester by harvesting crops which are not susceptible to Common Bunt, e.g. oats or any non-cereal crop (e.g. pea, soybean).
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identifying resistance genes in wheat against Common Bunt tilletia caries by use of virulence pattern of the pathogen
2019Co-Authors: Anders Borgen, Gunter Backes, Karl-josef Müller, Andrea Gallehr, Bettina Scherrer, Nanna Karkov Ytting, Hartmut SpiesAbstract:455 wheat varieties and breeding lines were grown in the field,contaminated with 7 to 11 different races of Common Bunt. Based on the reaction of the lines to the different virulence races, it was possible to group the lines by differential varieties with known resistance genes, indicating that they may have one or two of the resistance genes Bt1, Bt2, Bt5, Bt7, Bt13, BtZ or Quebon-resistance. Based hereof, genetic markers will be developed using a genome-wide association study (GWAS).
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evaluation of nordic heritage varieties and nils for resistance to Common Bunt tilletia caries syn t tritici
2018Co-Authors: Anders Borgen, Jan Svensson, Lars WiikAbstract:A number og wheat varieties from NordGen and Allkorn were tested fro susceptibility to Common Bunt. Most were susceptible to Common Bunt which is in line with other screening experiments of randomly selected varieties. However, a few lines not previously known to be resistant were shown to have different resistance genes. None of them however were resistant to all the virulence races tested. NILs developed by MacKey were evaluated and there seem to be a potential for using some of them as differential lines for resistance gene Bt1, Bt5, and Bt9. Purification based on resistant head rows may also develop NILs with resistance to Bt6 and Bt10 and possible also Bt7 from NGB16160. A crossing program of the remaining Bt-genes was started in 2016. The NILs may be used also to support development of genetic markers of the resistance genes.
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strategic use of virulence pattern to develop genetic markers for resistance to Common Bunt tilletia caries in wheat
2018Co-Authors: Anders Borgen, Gunter Backes, Karl-josef Müller, Hartmut SpiesAbstract:When assesssing races of Common Bunt for virulens pattern within a region, it is important to take into account that collected spores may represent a diverse population of different virulence races. When screening spores on a differential set of wheat lines with known resistance genes, a low infection rate on a resistant wheat variety does not necessarily demonstrate that virulence is absent in the spore collection, but could be a sign that virulence is present, but only present in a low frequency among the spores. If just a few spores within a spore sample are indeed virulent, they may infect some plants and from there multiply the virulence quite rapidly next years. Previous studies have shown that virulence against most resistance genes were present in Denmark after purifying races of Common Bunt (Tilletia caries) on resistant varieties. So far, only wheat differential varieties with Bt4, Bt6, Bt9, Bt11 and Bt12 cannot be infected with Bunt races purified from Danish collections [1, and later own unpublished data]. Virulence against Bt4, Bt6 and Bt9 has been found in other European studies [2], and Bt11 may not be only one gene but a combination of at least two genes [3]. Therefore, Bt12 seems to be the only gene for which virulence have not been found in European population of Common Bunt. This leads to the conclusion that if resistance breeding shall safely control Common Bunt in wheat, we need not only one effective gene, but a combination of pyramided genes. Since it is very difficult to test if a resistant line has only one gene or more genes, the most effective tool to achieve this at present are genetic markers. Using Genome Wide Association Studies (GWAS) to find QTLs and markers for the major resistance genes in wheat have so far led to only few commercial useful markers. Till now, only markers for Bt9 [6] and Bt10 are used in practice, but a marker for Bt12 [4] and Blizzard [7] have also been found. One of the problems in developing markers for Bunt resistance have been that spores used in GWAS trials have been divers or unknown in virulence, and that phenotypic results not distinguishes between different resistance genes. Therefore, the most successful studies have used segregating populations of single crosses where the resistance gene is known on before hand [5]. In the LIVESEED project, we have the ambition to develop genetic markers on several different resistance genes at the same time. We will do so by testing segregating populations of several different crosses between varieties with 7 different resistance genes, and infect them with 7-11 different virulence races of Common Bunt able to distinguish between the resistance genes. A total of 300 varieties will be pheno- and genotyped. Using this experimental design, we attempt during 2018 and ‘19 to develop markers for Bt1, Bt2, Bt5, Bt7, Bt13, BtZ and Quebon-resistance, and hopefully also a couple of minor QTLs.
Arti Singh - One of the best experts on this subject based on the ideXlab platform.
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Mapping quantitative trait loci associated with Common Bunt resistance in a spring wheat (Triticum aestivum L.) variety Lillian
Theoretical and Applied Genetics, 2019Co-Authors: Firdissa E. Bokore, Ron E. Knox, Heather L. Campbell, Amidou N’diaye, Arti Singh, Richard D. Cuthbert, Andrew G Sharpe, Curtis J. Pozniak, Yuefeng RuanAbstract:Key messageBased on their consistency over environments, two QTL identified in Lillian on chromosomes 5A and 7A could be useful targets for marker assisted breeding of Common Bunt resistance.AbstractCommon Bunt of wheat ( Triticum aestivum L.) caused by Tilletia tritici and T. laevis is an economically important disease because of losses in grain yield and reduced grain quality. Resistance can be quantitative, under the control of multiple small effect genes. The Canada Western Red Spring wheat variety Lillian is moderately resistant to Common Bunt races found on the Canadian prairies. This study was conducted to identify and map quantitative trait loci (QTL) conferring resistance against Common Bunt in Lillian. A doubled haploid population comprising 280 lines was developed from F _1 plants of the cross of Lillian by Vesper. The lines were inoculated at seeding with the two races L16 ( T. laevis ) and T19 ( T. tritici ), grown in field near Swift Current, SK, in 2014, 2015 and 2016 and assessed for disease incidence. The lines were genotyped with the 90 K iSelect SNP genotyping assay, and a high-density genetic map was constructed. Quantitative trait locus analysis was performed with MapQTL.6^® software. Two relatively stable Common Bunt resistance QTL, detected in two of the 3 years, were identified on chromosomes 5A and 7A from Lillian. In addition, three less stable QTL, appearing in one out of 3 years, were identified: one was contributed by Lillian on chromosome 3D and two were contributed by Vesper on chromosomes 1D and 2A. Epistatic interaction was identified for the Bunt incidence between 3D and 7A resulting in greater Bunt resistance. Future Bunt resistance breeding will benefit from combining these QTL through gene pyramiding.
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mapping quantitative trait loci associated with Common Bunt resistance in a spring wheat triticum aestivum l variety lillian
Theoretical and Applied Genetics, 2019Co-Authors: Firdissa E. Bokore, Arti Singh, Richard D. Cuthbert, Andrew G Sharpe, Curtis J. Pozniak, H. L. Campbell, R E Knox, Amidou Ndiaye, Yuefeng RuanAbstract:Based on their consistency over environments, two QTL identified in Lillian on chromosomes 5A and 7A could be useful targets for marker assisted breeding of Common Bunt resistance. Common Bunt of wheat (Triticum aestivum L.) caused by Tilletia tritici and T. laevis is an economically important disease because of losses in grain yield and reduced grain quality. Resistance can be quantitative, under the control of multiple small effect genes. The Canada Western Red Spring wheat variety Lillian is moderately resistant to Common Bunt races found on the Canadian prairies. This study was conducted to identify and map quantitative trait loci (QTL) conferring resistance against Common Bunt in Lillian. A doubled haploid population comprising 280 lines was developed from F1 plants of the cross of Lillian by Vesper. The lines were inoculated at seeding with the two races L16 (T. laevis) and T19 (T. tritici), grown in field near Swift Current, SK, in 2014, 2015 and 2016 and assessed for disease incidence. The lines were genotyped with the 90 K iSelect SNP genotyping assay, and a high-density genetic map was constructed. Quantitative trait locus analysis was performed with MapQTL.6® software. Two relatively stable Common Bunt resistance QTL, detected in two of the 3 years, were identified on chromosomes 5A and 7A from Lillian. In addition, three less stable QTL, appearing in one out of 3 years, were identified: one was contributed by Lillian on chromosome 3D and two were contributed by Vesper on chromosomes 1D and 2A. Epistatic interaction was identified for the Bunt incidence between 3D and 7A resulting in greater Bunt resistance. Future Bunt resistance breeding will benefit from combining these QTL through gene pyramiding.
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Genetic mapping of Common Bunt resistance and plant height QTL in wheat
Theoretical and Applied Genetics, 2016Co-Authors: Arti Singh, Ron E. Knox, Richard D. Cuthbert, R. M. Depauw, A. K. Singh, S. Kumar, H. L. CampbellAbstract:Key message Breeding for field resistance to Common Bunt in wheat will need to account for multiple genes and epistatic and QTL by environment interactions. Loci associated with quantitative resistance to Common Bunt are co-localized with other beneficial traits including plant height and rust resistance. Abstract Common Bunt, also known as stinking smut, is caused by seed borne fungi Tilletia tritici (Bjerk.) Wint. [syn. Tilletia caries (DC.) Tul.] and Tilletia laevis Kühn [syn. Tilletia foetida (Wallr.) Liro.]. Common Bunt is known to cause grain yield and quality losses in wheat due to Bunt ball formation and infestation of the grain. The objectives of this research were to identify and map quantitative trait loci (QTL) for Common Bunt resistance, to study the epistatic interactions between the identified QTL, and investigate the co-localization of Bunt resistance with plant height. A population of 261 doubled haploid lines from the cross Carberry/AC Cadillac and checks were genotyped with polymorphic genome wide microsatellite and DArT^® markers. The lines were grown in 2011, 2012, and 2013 in separate nurseries for Common Bunt incidence and height evaluation. AC Cadillac contributed a QTL ( QCbt.spa - 6D ) for Common Bunt resistance on chromosome 6D at markers XwPt - 1695 , XwPt - 672044 , and XwPt - 5114. Carberry contributed QTL for Bunt resistance on chromosomes 1B ( QCbt.spa - 1B at XwPt743523 ) 4B ( QCbt.spa - 4B at XwPt - 744434 - Xwmc617 ), 4D ( QCbt.spa - 4D at XwPt - 9747), 5B ( QCbt.spa - 5B at XtPt - 3719 ) and 7D ( QCbt.spa - 7D at Xwmc273 ). Significant epistatic interactions were identified for percent Bunt incidence between QCbt.spa - 1B × QCbt.spa - 4B and QCbt.spa - 1B × QCbt.spa - 6D, and QTL by environment interaction between QCbt.spa - 1B × QCbt.spa - 6D . Plant height QTL were found on chromosomes 4B ( QPh.spa - 4B ) and 6D ( QPh.spa - 6D ) that co-located with Bunt resistance QTL. The identification of previously unreported Common Bunt resistance QTL (on chromosomes 4B, 4D and 7D), and new understanding of QTL × QTL interactions will facilitate marker-assisted breeding for Common Bunt resistance.
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genetic mapping of Common Bunt resistance and plant height qtl in wheat
Theoretical and Applied Genetics, 2016Co-Authors: Arti Singh, Richard D. Cuthbert, R. M. Depauw, A. K. Singh, S. Kumar, R E Knox, H. L. CampbellAbstract:Key message Breeding for field resistance to Common Bunt in wheat will need to account for multiple genes and epistatic and QTL by environment interactions. Loci associated with quantitative resistance to Common Bunt are co-localized with other beneficial traits including plant height and rust resistance.
Yuefeng Ruan - One of the best experts on this subject based on the ideXlab platform.
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Mapping quantitative trait loci associated with Common Bunt resistance in a spring wheat (Triticum aestivum L.) variety Lillian
Theoretical and Applied Genetics, 2019Co-Authors: Firdissa E. Bokore, Ron E. Knox, Heather L. Campbell, Amidou N’diaye, Arti Singh, Richard D. Cuthbert, Andrew G Sharpe, Curtis J. Pozniak, Yuefeng RuanAbstract:Key messageBased on their consistency over environments, two QTL identified in Lillian on chromosomes 5A and 7A could be useful targets for marker assisted breeding of Common Bunt resistance.AbstractCommon Bunt of wheat ( Triticum aestivum L.) caused by Tilletia tritici and T. laevis is an economically important disease because of losses in grain yield and reduced grain quality. Resistance can be quantitative, under the control of multiple small effect genes. The Canada Western Red Spring wheat variety Lillian is moderately resistant to Common Bunt races found on the Canadian prairies. This study was conducted to identify and map quantitative trait loci (QTL) conferring resistance against Common Bunt in Lillian. A doubled haploid population comprising 280 lines was developed from F _1 plants of the cross of Lillian by Vesper. The lines were inoculated at seeding with the two races L16 ( T. laevis ) and T19 ( T. tritici ), grown in field near Swift Current, SK, in 2014, 2015 and 2016 and assessed for disease incidence. The lines were genotyped with the 90 K iSelect SNP genotyping assay, and a high-density genetic map was constructed. Quantitative trait locus analysis was performed with MapQTL.6^® software. Two relatively stable Common Bunt resistance QTL, detected in two of the 3 years, were identified on chromosomes 5A and 7A from Lillian. In addition, three less stable QTL, appearing in one out of 3 years, were identified: one was contributed by Lillian on chromosome 3D and two were contributed by Vesper on chromosomes 1D and 2A. Epistatic interaction was identified for the Bunt incidence between 3D and 7A resulting in greater Bunt resistance. Future Bunt resistance breeding will benefit from combining these QTL through gene pyramiding.
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mapping quantitative trait loci associated with Common Bunt resistance in a spring wheat triticum aestivum l variety lillian
Theoretical and Applied Genetics, 2019Co-Authors: Firdissa E. Bokore, Arti Singh, Richard D. Cuthbert, Andrew G Sharpe, Curtis J. Pozniak, H. L. Campbell, R E Knox, Amidou Ndiaye, Yuefeng RuanAbstract:Based on their consistency over environments, two QTL identified in Lillian on chromosomes 5A and 7A could be useful targets for marker assisted breeding of Common Bunt resistance. Common Bunt of wheat (Triticum aestivum L.) caused by Tilletia tritici and T. laevis is an economically important disease because of losses in grain yield and reduced grain quality. Resistance can be quantitative, under the control of multiple small effect genes. The Canada Western Red Spring wheat variety Lillian is moderately resistant to Common Bunt races found on the Canadian prairies. This study was conducted to identify and map quantitative trait loci (QTL) conferring resistance against Common Bunt in Lillian. A doubled haploid population comprising 280 lines was developed from F1 plants of the cross of Lillian by Vesper. The lines were inoculated at seeding with the two races L16 (T. laevis) and T19 (T. tritici), grown in field near Swift Current, SK, in 2014, 2015 and 2016 and assessed for disease incidence. The lines were genotyped with the 90 K iSelect SNP genotyping assay, and a high-density genetic map was constructed. Quantitative trait locus analysis was performed with MapQTL.6® software. Two relatively stable Common Bunt resistance QTL, detected in two of the 3 years, were identified on chromosomes 5A and 7A from Lillian. In addition, three less stable QTL, appearing in one out of 3 years, were identified: one was contributed by Lillian on chromosome 3D and two were contributed by Vesper on chromosomes 1D and 2A. Epistatic interaction was identified for the Bunt incidence between 3D and 7A resulting in greater Bunt resistance. Future Bunt resistance breeding will benefit from combining these QTL through gene pyramiding.
Curtis J. Pozniak - One of the best experts on this subject based on the ideXlab platform.
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Mapping quantitative trait loci associated with Common Bunt resistance in a spring wheat (Triticum aestivum L.) variety Lillian
Theoretical and Applied Genetics, 2019Co-Authors: Firdissa E. Bokore, Ron E. Knox, Heather L. Campbell, Amidou N’diaye, Arti Singh, Richard D. Cuthbert, Andrew G Sharpe, Curtis J. Pozniak, Yuefeng RuanAbstract:Key messageBased on their consistency over environments, two QTL identified in Lillian on chromosomes 5A and 7A could be useful targets for marker assisted breeding of Common Bunt resistance.AbstractCommon Bunt of wheat ( Triticum aestivum L.) caused by Tilletia tritici and T. laevis is an economically important disease because of losses in grain yield and reduced grain quality. Resistance can be quantitative, under the control of multiple small effect genes. The Canada Western Red Spring wheat variety Lillian is moderately resistant to Common Bunt races found on the Canadian prairies. This study was conducted to identify and map quantitative trait loci (QTL) conferring resistance against Common Bunt in Lillian. A doubled haploid population comprising 280 lines was developed from F _1 plants of the cross of Lillian by Vesper. The lines were inoculated at seeding with the two races L16 ( T. laevis ) and T19 ( T. tritici ), grown in field near Swift Current, SK, in 2014, 2015 and 2016 and assessed for disease incidence. The lines were genotyped with the 90 K iSelect SNP genotyping assay, and a high-density genetic map was constructed. Quantitative trait locus analysis was performed with MapQTL.6^® software. Two relatively stable Common Bunt resistance QTL, detected in two of the 3 years, were identified on chromosomes 5A and 7A from Lillian. In addition, three less stable QTL, appearing in one out of 3 years, were identified: one was contributed by Lillian on chromosome 3D and two were contributed by Vesper on chromosomes 1D and 2A. Epistatic interaction was identified for the Bunt incidence between 3D and 7A resulting in greater Bunt resistance. Future Bunt resistance breeding will benefit from combining these QTL through gene pyramiding.
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mapping quantitative trait loci associated with Common Bunt resistance in a spring wheat triticum aestivum l variety lillian
Theoretical and Applied Genetics, 2019Co-Authors: Firdissa E. Bokore, Arti Singh, Richard D. Cuthbert, Andrew G Sharpe, Curtis J. Pozniak, H. L. Campbell, R E Knox, Amidou Ndiaye, Yuefeng RuanAbstract:Based on their consistency over environments, two QTL identified in Lillian on chromosomes 5A and 7A could be useful targets for marker assisted breeding of Common Bunt resistance. Common Bunt of wheat (Triticum aestivum L.) caused by Tilletia tritici and T. laevis is an economically important disease because of losses in grain yield and reduced grain quality. Resistance can be quantitative, under the control of multiple small effect genes. The Canada Western Red Spring wheat variety Lillian is moderately resistant to Common Bunt races found on the Canadian prairies. This study was conducted to identify and map quantitative trait loci (QTL) conferring resistance against Common Bunt in Lillian. A doubled haploid population comprising 280 lines was developed from F1 plants of the cross of Lillian by Vesper. The lines were inoculated at seeding with the two races L16 (T. laevis) and T19 (T. tritici), grown in field near Swift Current, SK, in 2014, 2015 and 2016 and assessed for disease incidence. The lines were genotyped with the 90 K iSelect SNP genotyping assay, and a high-density genetic map was constructed. Quantitative trait locus analysis was performed with MapQTL.6® software. Two relatively stable Common Bunt resistance QTL, detected in two of the 3 years, were identified on chromosomes 5A and 7A from Lillian. In addition, three less stable QTL, appearing in one out of 3 years, were identified: one was contributed by Lillian on chromosome 3D and two were contributed by Vesper on chromosomes 1D and 2A. Epistatic interaction was identified for the Bunt incidence between 3D and 7A resulting in greater Bunt resistance. Future Bunt resistance breeding will benefit from combining these QTL through gene pyramiding.
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Mapping of QTLs associated with resistance to Common Bunt, tan spot, leaf rust, and stripe rust in a spring wheat population
Molecular Breeding, 2017Co-Authors: Kassa Semagn, Curtis J. Pozniak, Hua Chen, Muhammad Iqbal, Mohammad Asif, Amidou N’diaye, Alireza Navabi, Enid Perez-lara, Rong-cai Yang, Robert J. GrafAbstract:Spring wheat ( Triticum aestivum L.) breeding goals in western Canada include good agronomic characteristics and good end-use quality, and also moderate to elevated resistance to diseases of economic importance. In this study, we aimed to identify quantitative trait loci (QTL) associated with resistance to Common Bunt ( Tilletia tritici and Tilletia laevis ), tan spot ( Pyrenophora tritici-repentis ), leaf rust ( Puccinia triticina ), and stripe rust ( Puccinia striiformis f. sp. tritici ). A total of 167 recombinant inbred lines (RILs) derived from a cross between two spring wheat cultivars, ‘Attila’ and ‘CDC Go’, were evaluated for reactions to the four diseases in nurseries from three to eight environments, and genotyped with the Wheat 90K SNP array and three gene-specific markers ( Ppd-D1 , Vrn-A1 , and Rht-B1 ). The RILs exhibited transgressive segregation for all four diseases, and we observed several lines either superior or inferior to the parents. Broad-sense heritability varied from 0.25 for leaf rust to 0.48 for Common Bunt. Using a subset of 1203 informative markers (1200 SNPs and 3 gene-specific markers) and average disease scores across all environments, we identified two QTLs ( QCbt.dms-1B.2 and QCbt.dms-3A ) for Common Bunt, and three QTLs each for tan spot ( QTs.dms-2B , QTs.dms-2D , and QTs.dms-6B ), leaf rust ( QLr.dms-2D.1 , QLr.dms-2D.2 , and QLr.dms-3A ), and stripe rust ( QYr.dms-3A , QYr.dms-4A , and QYr.dms-5B ). Each QTL individually explained between 5.9 and 18.7% of the phenotypic variation, and altogether explained from 21.5 to 26.5% of phenotypic and from 52.2 to 86.0% of the genetic variation. The resistance alleles for all QTLs except one for stripe rust ( QYr.dms-5B ) were from CDC Go. Some of the QTLs are novel, while others mapped close to QTLs and/or genes reported in other studies.