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Chiaki Kamei - One of the best experts on this subject based on the ideXlab platform.
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Effect of topical application of Brazilian propolis on scratching behavior induced by Compound 48/80 in mice.
Immunopharmacology and immunotoxicology, 2010Co-Authors: Yoshifumi Shinmei, Maria Alejandra Hossen, Yoto Kagawa, Haruna Yano, Chiaki KameiAbstract:Background and aim: We investigated the effect of topical application of Brazilian propolis on scratching behavior induced by Compound 48/80 in mice.Results: Propolis inhibited Compound 48/80-induced scratching behavior when applied immediately after treatment with propolis at a dose of 3 mg/site. Dibucaine 0.3 mg/site also significantly inhibited Compound 48/80-induced scratching behavior immediately after application. On the other hand, propolis inhibited Compound 48/80-induced scratching behavior even 15, 30 and 60 min after application; however, dibucaine showed no significant inhibition of Compound 48/80-induced scratching behavior 15, 30 and 60 min after application. In addition, propolis had no effect on increased vascular permeability just after application, but the drug had a significant effect 15, 30 and 60 min after application. On the contrary, histamine-induced scratching behavior was inhibited significantly by propolis just after application. On the other hand, propolis significantly inhibit...
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Caffeic acid inhibits Compound 48/80-induced allergic symptoms in mice.
Biological & pharmaceutical bulletin, 2006Co-Authors: Maria Alejandra Hossen, Toshio Inoue, Yoshifumi Shinmei, Kazuhisa Minami, Yoko Fujii, Chiaki KameiAbstract:The effect of caffeic acid on scratching behavior and vascular permeability changes induced by Compound 48/80 in ICR mice were investigated. An oral dose of 500 mg/kg of caffeic acid significantly inhibited scratching behavior and vascular permeability induced by Compound 48/80. The inhibitory effects of daily administration of lower doses of caffeic acid, 100 and 200 mg/kg, were also investigated; and it was found that 200 mg/kg significantly inhibited Compound 48/80-induced scratching behavior after the second week of consecutive administration. The effect of 200 mg/kg of caffeic acid on scratching behavior was observed up to the third week of the treatment. The decrease in histamine content induced by Compound 48/80 was significantly antagonized by 200 mg/kg. The findings suggest that caffeic acid may be effective for treating itch and edema in allergic dermatitis.
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Effect of Brazilian propolis on scratching behavior induced by Compound 48/80 and histamine in mice.
International Immunopharmacology, 2004Co-Authors: Yoshifumi Shinmei, Maria Alejandra Hossen, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada, Chiaki KameiAbstract:We studied the effect of Brazilian propolis on scratching behavior induced by Compound 48/80 and histamine in ICR mice. Propolis granular A.P.C dose-related inhibited scratching behavior induced by Compound 48/80 and significant inhibition were observed at 1000 mg/kg. However, histamine-induced scratching behavior was not inhibited by propolis granular A.P.C even at 1000 mg/kg. Propolis ethanol extract at 10 μg/ml or more inhibited histamine release from rat mast cells induced by Compound 48/80. In addition, it blocked increased vascular permeability induced by Compound 48/80. The inhibitory effect of propolis on scratching behavior induced by Compound 48/80 was gradually enhanced by repeated administration, and 500 mg/kg propolis granular A.P.C, which caused no effect through single administration, significantly inhibited scratching behavior after repeated administration for 4 weeks. From these findings, it is assumed that the inhibition of scratching behavior induced by propolis occurs through a mast cell-dependent mechanism.
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Changes in membrane potential induced by Compound 48/80 in the peritoneal mast cells of rats.
Methods and findings in experimental and clinical pharmacology, 2002Co-Authors: Yukinobu Nakayama, Yoko Fujii, Mitsunobu Mio, Yukio Sugimoto, Chiaki KameiAbstract:The changes in membrane potential induced by Compound 48/80 were studied using rat peritoneal mast cells. The mean resting membrane potential of rat mast cells was 12.3 ± 0.7 mM. When Compound 48/80 was added to the mast cells the cells were degranulated approximately 120 sec after the addition of the drug, after which immediate depolarization occurred. Degranulation of mast cells was not observed, even under the depolarization or hyperpolarization conditions caused by the replacement of a high K + medium or the removal of K + from the medium, respectively Under both conditions, when Compound 48/80 was added to the mast cells, degranulation was observed. Abrupt and marked depolarization was induced 30-60 sec after Compound 48/80 was added. In addition, repolarization followed by gradual depolarization was observed without degranulation in mast cells treated with cytochalasin D after the addition of Compound 48/80. These results suggest that the mast cells were depolarized by Compound 48/80 independently of degranulation. It is also feasible that the gradual depolarization and repolarization induced by Compound 48/80 in mast cell pretreated with cytochalasin D participated in the extracellular Na + and Na + /K + -pump, respectively.
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Effects of histamine H1 receptor antagonists on Compound 48/80-induced scratching behavior in mice
European journal of pharmacology, 1998Co-Authors: Yukio Sugimoto, Keiko Umakoshi, Nao Nojiri, Chiaki KameiAbstract:Abstract The effects of histamine H 1 receptor antagonists on Compound 48/80-induced scratching behavior were studied in mice. Classical histamine H 1 receptor antagonists such as diphenhydramine and chlorpheniramine caused a potent depressant effect on Compound 48/80-induced scratching behavior. Histamine H 1 receptor antagonists having antiallergic activity (an inhibition of mast cell degranulation), such as azelastine and oxatomide and nonsedative histamine H 1 receptor antagonists such as terfenadine, epinastine and astemizole, also showed a relatively potent effect. On the other hand, the effects of tranilast and cromolyn sodium—antiallergic drugs without histamine H 1 receptor antagonistic activity—were extremely weak. Diazepam had weak or no depressant effects on Compound 48/80-induced scratching behavior. These results suggest that inhibition of Compound 48/80-induced scratching behavior is mainly due to histamine H 1 receptor antagonistic activity and not to the sedative action of the drugs.
Chang Ho Song - One of the best experts on this subject based on the ideXlab platform.
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Lipoic acid suppresses Compound 48/80-induced anaphylaxis-like reaction
Anatomy & cell biology, 2010Co-Authors: Yun Ho Choi, Ok Hee Chai, Eui-hyeog Han, Su-young Choi, Hyoung Tae Kim, Chang Ho SongAbstract:Alpha-lipoic acid (LA), a naturally occurring dithiol Compound, is an essential cofactor in metabolic reactions involved in energy utilization. LA improves glycemic control, reduces diabetic polyneuropathies, atherosclerosis, and allergic inflammation. The effects of LA on mast cell-mediated anaphylactic reactions, however, are unknown. LA dose-dependently inhibited systemic and passive cutaneous anaphylaxis-like reactions in mice induced by Compound 48/80, a condensation product of N-methyl-p-methoxyphenethylamine and formaldehyde. Pretreatment with LA, prior to induction of the systemic anaphylaxis-like reaction with Compound 48/80, reduced plasma histamine levels in a dose-dependent manner. In our in vitro study, LA decreased histamine release from rat peritoneal mast cells (RPMCs) triggered by Compound 48/80. Moreover, an increase in calcium uptake activated by Compound 48/80 was inhibited by LA. LA also significantly elevated intracellular cyclic adenosine-3',5' monophosphate (cAMP) levels in RPMCs. This inhibition of mediator release from RPMCs may be due to inhibition of calcium uptake and augmentation of intracellular cAMP levels. Based on these results, we suggest that LA may be a potential remedy for allergy-related diseases.
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Inhibitory effects of curcumin on passive cutaneous anaphylactoid response and Compound 48/80-induced mast cell activation
Anatomy & cell biology, 2010Co-Authors: Yun Ho Choi, Ok Hee Chai, Guang-hai Yan, Chang Ho SongAbstract:Mast cells participate in allergies and inflammation by secreting a variety of pro-inflammatory mediators. Curcumin, the active component of turmeric, is a polyphenolic phytochemical with anti-tumor, anti-inflammatory, anti-oxidative, and anti-allergic properties. The effects of curcumin on Compound 48/80-induced mast cell activation and passive cutaneous anaphylactoid reactions are unknown. In this report, we investigated the influences of curcumin on the passive cutaneous anaphylactoid response in vivo and Compound 48/80-induced mast cell activation in vitro. The mechanism of action was examined by calcium uptake measurements and cAMP assays in mast cells. Curcumin significantly attenuated the mast cell-mediated passive cutaneous anaphylactoid reaction in an animal model. In agreement with this in vivo activity, curcumin suppressed Compound 48/80-induced rat peritoneal mast cell (RPMC) degranulation and histamine release from RPMCs. Moreover, Compound 48/80-elicited calcium uptake into RPMCs was reduced in a dose-dependent manner by curcumin. Furthermore, curcumin increased the level of intracellular cAMP and significantly inhibited the Compound 48/80-induced reduction of cAMP in RPMCs. These results corroborate the finding that curcumin may have anti-allergic activity.
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Inhibitory effects of epigallocatechin gallate on Compound 48/80-induced mast cell activation and passive cutaneous anaphylaxis.
Experimental & molecular medicine, 2005Co-Authors: Ok Hee Chai, Chang Ho SongAbstract:Epigallocatechin gallate (EGCG) is a principle phenolic antioxidant found in a variety of plants, including green and black tea. The anti-allergic effect of EGCG is unknown. The purpose of this study is to investigate the effects of EGCG on Compound 48/80-induced mast cell activation and passive cutaneous anaphylaxis. For this, the influences of EGCG on the Compound 48/80-induced cutaneous reaction were measured in vivo and the effects of EGCG on the Compound 48/80-induced mast cell activations were examined in vitro. Results are below: as 1) EGCG significantly inhibited Compound 48/80-induced passive cutaneous anaphylaxis, 2) the Compound 48/80-induced degranulation, calcium influx and histamine release of rat peritoneal mast cells (RPMCs) were significantly inhibited by the pretreatment with EGCG, and 3) the Compound 48/80-mediated inhibition of cAMP level in RPMCs was significantly increased by the pretreatment with EGCG. These results suggested that EGCG, the most abundant polyphenol in green tea, inhibits the Compound 48/80-induced mast cell activation and the increase of vascular permeability, and potentially serve as effective therapeutic tools for allergic diseases.
Hannu Uusitalo - One of the best experts on this subject based on the ideXlab platform.
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Intraocular effects of ruthenium red in responses to Compound 48/80 and topical formaldehyde in rabbit.
Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and Therapeutics, 1996Co-Authors: H. Stenbäck, Kari Krootila, Arto Palkama, Hannu UusitaloAbstract:ABSTRACT We have previously shown the presence and localization of mast cells and the intraocular effects of Compound 48/80 in the rabbit eye. In the present study we have evaluated the mechanism of action of Compound 48/80 using ruthenium red as a blocker of sensory axon reflexes in the rabbit eye and by measuring the intraocular pressure (IOP), the pupil size, the blood pressure, the protein and cAMP content in the aqueous humour. Topical neutral formaldehyde was used as a topical inducer of neuronally mediated response in a separate series of experiment. Intracamerally-injected ruthenium red suppressed the Compound 48/80-induced elevation intraocular pressure and prevented miosis, while having little if any effect on the breakdown of the blood-aqueous barrier and on the increase in the cAMP concentration in aqueous humour. Ruthenium red also inhibited the irritative response in eyes treated with topical 1% formaldehyde. As the blood-aqueous barrier in the rabbit is an extremely sensitive system higher ...
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Mast cells in the anterior uvea of the rabbit. Intraocular effects of Compound 48/80 in the rabbit
Experimental Eye Research, 1992Co-Authors: H. Stenbäck, Kari Krootila, Arto Palkama, Hannu UusitaloAbstract:In the present study, the presence and localization of mast cells and the intraocular effects of Compound 48/80 have been studied in detail in the rabbit eye using histochemical and physiological methods. In histochemical studies mast cells were localized in the anterior uvea, especially in the ciliary and iridial processes. Intracamerally injected, Compound 48/80 caused an increase in the intraocular pressure, disruption of the blood-aqueous barrier and an increase in the cAMP content in the aqueous humour. Miosis was observed only after higher doses of Compound 48/80 (> 100 μ g)and even then only one-half of the eyes responded. The intraocular effects, excluding miosis, of Compound 48/80 resembled an on/off-type of response, where 20 μ g caused only minorchanges, if any, and 50 μ g gave a maximal response. The ocular hypertensive reaction developed a tachyphylaxis so that the second and third consecutive dose of Compound 48/80 (100 μ g) produced no significant change in IOP. The results indicate that mast cells, which are present in the anterior uvea in an extent not knownpreviously, might be involved in certain inflammatory reactions in the rabbit eye. The inconsistent and slight miosis after the intracameral application of Compound 48/80 indicates that the mechanism is different from that caused by sensory nerve stimulation. The rapid development of tachyphylaxis after consecutive application of Compound 48/80 suggests that mast cells are easily depleted which might be useful for further studies to evaluate the functional role of mast cellsin different pathophysiological conditions in the rabbit eye.
Kurt J. Bloch - One of the best experts on this subject based on the ideXlab platform.
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Ocular anaphylaxis induced in the rat by topical application of Compound 48/80. Dose response and time course study.
Acta Ophthalmologica, 2009Co-Authors: Mathea R. Allansmith, Robert S. Baird, Robert N. Ross, Neal P. Barney, Kurt J. BlochAbstract:In the present study we sought to develop a model of ocular anaphylaxis based on the topical application of Compound 48/80 to the surface of the rat eye. Doses ranging from 50 to 1000 micrograms were found to produce graded edema of the conjunctiva and swelling of the lid. On histologic examination, 50 microns Compound 48/80 produced no changes distinguishable from those in PBS-treated controls, 150 microns produced mild alterations, and 250, 500, and 1000 micrograms Compound 48/80 produced a marked increase in degranulated mast cells and a mild influx of neutrophils. The time course of the response to 250 micrograms and 1000 micrograms of Compound 48/80 was evaluated over a 72-h period. Both doses elicited epithelial damage. A mild reduction in the number of mast cell was seen at 6 h in rats receiving 250 or 1000 micrograms. The reduction persisted to 72 h in rats receiving 1000 micrograms. The number of neutrophils was increased at 1 and 6 h in eyes treated with 250 micrograms and at 1, 6, and 24 h in eyes treated with 1000 micrograms Compound 48/80. The clinical and histologic changes induced by application of 250 micrograms Compound 48/80 resemble those seen in patients with allergic conjunctivitis suggesting that a model of ocular anaphylaxis based on the topical application Compound 48/80 will be clinically relevant and experimentally practical.
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Effect of multiple applications of Compound 48/80 on mast cells of rat conjunctiva.
Acta ophthalmologica, 2009Co-Authors: Mathea R. Allansmith, Robert S. Baird, Robert N. Ross, Neal P. Barney, Kurt J. BlochAbstract:We sought to determine the effect on rat conjunctival mast cells of chronic exposure to a degranulating agent. Compound 48/80 (250 micrograms in 10 microliter) was applied daily to the ocular surface of rats for a total of 13 treatments administered in 17 days. A single application of Compound 48/80 was given to rats for comparison. Four groups of animals (6 in each group) were evaluated 1 or 48 h after the last application of Compound 48/80 in both chronic exposure and single exposure studies. Although a single exposure to Compound 48/80 induced significant degranulation of mast cells in animals observed 1 h after treatment, no significant degranulation of mast cells was observed 1 h after the last dose of Compound 48/80 was given to rats whose conjunctiva had been chronically exposed to this degranulating agent. Thus, multiple applications of Compound 48/80 attenuated the mast cell response to Compound 48/80. This acquired tolerance to Compound 48/80 was reflected in the lessened clinical signs in rats receiving repeated applications compared with those given a single application.
Yoshifumi Shinmei - One of the best experts on this subject based on the ideXlab platform.
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Effect of topical application of Brazilian propolis on scratching behavior induced by Compound 48/80 in mice.
Immunopharmacology and immunotoxicology, 2010Co-Authors: Yoshifumi Shinmei, Maria Alejandra Hossen, Yoto Kagawa, Haruna Yano, Chiaki KameiAbstract:Background and aim: We investigated the effect of topical application of Brazilian propolis on scratching behavior induced by Compound 48/80 in mice.Results: Propolis inhibited Compound 48/80-induced scratching behavior when applied immediately after treatment with propolis at a dose of 3 mg/site. Dibucaine 0.3 mg/site also significantly inhibited Compound 48/80-induced scratching behavior immediately after application. On the other hand, propolis inhibited Compound 48/80-induced scratching behavior even 15, 30 and 60 min after application; however, dibucaine showed no significant inhibition of Compound 48/80-induced scratching behavior 15, 30 and 60 min after application. In addition, propolis had no effect on increased vascular permeability just after application, but the drug had a significant effect 15, 30 and 60 min after application. On the contrary, histamine-induced scratching behavior was inhibited significantly by propolis just after application. On the other hand, propolis significantly inhibit...
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Caffeic acid inhibits Compound 48/80-induced allergic symptoms in mice.
Biological & pharmaceutical bulletin, 2006Co-Authors: Maria Alejandra Hossen, Toshio Inoue, Yoshifumi Shinmei, Kazuhisa Minami, Yoko Fujii, Chiaki KameiAbstract:The effect of caffeic acid on scratching behavior and vascular permeability changes induced by Compound 48/80 in ICR mice were investigated. An oral dose of 500 mg/kg of caffeic acid significantly inhibited scratching behavior and vascular permeability induced by Compound 48/80. The inhibitory effects of daily administration of lower doses of caffeic acid, 100 and 200 mg/kg, were also investigated; and it was found that 200 mg/kg significantly inhibited Compound 48/80-induced scratching behavior after the second week of consecutive administration. The effect of 200 mg/kg of caffeic acid on scratching behavior was observed up to the third week of the treatment. The decrease in histamine content induced by Compound 48/80 was significantly antagonized by 200 mg/kg. The findings suggest that caffeic acid may be effective for treating itch and edema in allergic dermatitis.
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Effect of Brazilian propolis on scratching behavior induced by Compound 48/80 and histamine in mice.
International Immunopharmacology, 2004Co-Authors: Yoshifumi Shinmei, Maria Alejandra Hossen, Kiyoshi Okihara, Hiroyuki Sugimoto, Hideo Yamada, Chiaki KameiAbstract:We studied the effect of Brazilian propolis on scratching behavior induced by Compound 48/80 and histamine in ICR mice. Propolis granular A.P.C dose-related inhibited scratching behavior induced by Compound 48/80 and significant inhibition were observed at 1000 mg/kg. However, histamine-induced scratching behavior was not inhibited by propolis granular A.P.C even at 1000 mg/kg. Propolis ethanol extract at 10 μg/ml or more inhibited histamine release from rat mast cells induced by Compound 48/80. In addition, it blocked increased vascular permeability induced by Compound 48/80. The inhibitory effect of propolis on scratching behavior induced by Compound 48/80 was gradually enhanced by repeated administration, and 500 mg/kg propolis granular A.P.C, which caused no effect through single administration, significantly inhibited scratching behavior after repeated administration for 4 weeks. From these findings, it is assumed that the inhibition of scratching behavior induced by propolis occurs through a mast cell-dependent mechanism.