The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform

Yong-hwan Lee - One of the best experts on this subject based on the ideXlab platform.

  • two Conidiation related zn ii 2cys6 transcription factor genes in the rice blast fungus
    Fungal Genetics and Biology, 2013
    Co-Authors: Hyunjung Chung, Jaehyuk Choi, Sookyoung Park, Junhyun Jeon, Yong-hwan Lee
    Abstract:

    Regulation of gene expression by transcription factors (TFs) helps plant pathogens to interact with the host plants and to sustain a pathogenic lifestyle in the environmental changes. Elucidating novel functions of TFs is, therefore, crucial for understanding pathogenesis mechanisms of plant pathogens. Magnaporthe oryzae, the rice blast pathogen, undergoes a series of developmental morphogenesis to complete its infection cycle. To understand TF genes implicated in pathogenic development of this fungus, two Zn(II)2Cys6 TF genes, MoCOD1 and MoCOD2, whose expression was notably induced during Conidiation, were functionally characterized. Targeted deletion of MoCOD1 resulted in defects in Conidiation and pathogenicity due to defects in appressorium formation and invasive growth within the host cells. MoCOD2 was also a critical regulator in Conidiation and pathogenicity, but not in conidial germination and appressorium formation. When rice plants were inoculated with conidia of the ΔMocod2 mutant, rapid accumulation of dark brown granules was observed around the infection sites in the plant cells and no visible disease symptom was incited. Taken together, both MoCOD1 and MoCOD2 play important roles in Conidiation and pathogenicity of the rice blast fungus.

  • Gene Expression Profiling during Conidiation in the Rice Blast Pathogen Magnaporthe oryzae
    PloS one, 2012
    Co-Authors: Kyoung Su Kim, Yong-hwan Lee
    Abstract:

    Conidiation of phytopathogenic fungi is a key developmental process that plays a central role in their life cycles and in epidemics. However, there is little information on Conidiation-induced molecular changes in the rice blast fungus Magnaporthe oryzae. As a first step to understand conidiogenesis in this fungus, we measured genome-wide gene expression profiles during Conidiation using a whole genome oligonucleotide microarray. At a two-fold expression difference, approximately 4.42% and 4.08% of genes were upregulated and downregulated, respectively, during Conidiation. The differentially expressed genes were functionally categorized by gene ontology (GO) term analysis, which demonstrated that the gene set encoded proteins that function in metabolism, cell wall biosynthesis, transcription, and molecule transport. To define the events of the complicated process of conidiogenesis, another set of microarray experiments was performed using a deletion mutant for MoHOX2, a stage-specific transcriptional regulator essential for conidial formation, which was expressed de novo in a Conidiation-specific manner in M. oryzae. Gene expression profiles were compared between the wild-type and the ΔMohox2 mutant during Conidiation. This analysis defined a common gene set that was upregulated in the wild-type and downregulated in the ΔMohox2 mutant during Conidiation; this gene set is expected to include Conidiation-related downstream genes of MoHOX2. We identified several hundred genes that are differentially-expressed during Conidiation; our results serve as an important resource for understanding the Conidiation, a process in M. oryzae, which is critical for disease development.

Chaoxi Luo - One of the best experts on this subject based on the ideXlab platform.

  • Development of rice Conidiation media for Ustilaginoidea virens
    PloS one, 2019
    Co-Authors: Yufu Wang, Junbin Huang, Fei Wang, Songlin Xie, Yi Liu, Weixiao Yin, Chaoxi Luo
    Abstract:

    Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate Conidiation of U. virens. Among the tested media, 0.10 g/ml panicle medium was most efficient for Conidiation. Whereas, some rice leaf media more effectively increased Conidiation than panicle media except 0.10 g/ml panicle medium, and certain non-filtered tissue media were better than their filtered counterparts. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. The rice tissue medium is also workable in inducing conidia for Conidiation-defective isolates. This method provides a foundation for the production of conidia by U. virens that will be widely applicable in pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

  • Development of rice Conidiation media for Ustilaginoidea virens
    2019
    Co-Authors: Yufu Wang, Junbin Huang, Fei Wang, Songlin Xie, Yi Liu, Weixiao Yin, Chaoxi Luo
    Abstract:

    Abstract Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate Conidiation of U. virens. Generally, rice leaf media more effectively increased Conidiation than panicle media, and certain non-filtered tissue media were better than their filtered counterparts. Among the tested media, the Indica rice leaf medium with 0.06 g/ml of Wanxian 98 leaf was most efficient for inducing Conidiation, and it was also usable for Conidiation-defective isolates. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. This method provides a foundation for the production of conidia in U. virens that will be widely applied in the pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

Junbin Huang - One of the best experts on this subject based on the ideXlab platform.

  • Development of rice Conidiation media for Ustilaginoidea virens
    PloS one, 2019
    Co-Authors: Yufu Wang, Junbin Huang, Fei Wang, Songlin Xie, Yi Liu, Weixiao Yin, Chaoxi Luo
    Abstract:

    Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate Conidiation of U. virens. Among the tested media, 0.10 g/ml panicle medium was most efficient for Conidiation. Whereas, some rice leaf media more effectively increased Conidiation than panicle media except 0.10 g/ml panicle medium, and certain non-filtered tissue media were better than their filtered counterparts. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. The rice tissue medium is also workable in inducing conidia for Conidiation-defective isolates. This method provides a foundation for the production of conidia by U. virens that will be widely applicable in pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

  • Development of rice Conidiation media for Ustilaginoidea virens
    2019
    Co-Authors: Yufu Wang, Junbin Huang, Fei Wang, Songlin Xie, Yi Liu, Weixiao Yin, Chaoxi Luo
    Abstract:

    Abstract Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate Conidiation of U. virens. Generally, rice leaf media more effectively increased Conidiation than panicle media, and certain non-filtered tissue media were better than their filtered counterparts. Among the tested media, the Indica rice leaf medium with 0.06 g/ml of Wanxian 98 leaf was most efficient for inducing Conidiation, and it was also usable for Conidiation-defective isolates. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. This method provides a foundation for the production of conidia in U. virens that will be widely applied in the pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

  • phosphoribosylamidotransferase the first enzyme for purine de novo synthesis is required for Conidiation in the sclerotial mycoparasite coniothyrium minitans
    Fungal Genetics and Biology, 2011
    Co-Authors: Li Qin, Xiaoyan Gong, Daohong Jiang, Jiatao Xie, Jiasen Cheng, Junbin Huang
    Abstract:

    Coniothyrium minitans is an important sclerotial parasite of the fungal phytopathogen, Sclerotinia sclerotiorum. Previously, we constructed a T-DNA insertional library, and screened for many Conidiation-deficient mutants from this library. Here, we report a T-DNA insertional mutant ZS-1T21882 that completely lost Conidiation. In mutant ZS-1T21882, the T-DNA was integrated into a gene (CmPrat-1) which encodes phosphoribosylamidotransferase (PRAT, EC 2.4.2.14), an enzyme catalyzing the first committed step in de novo purine nucleotide synthesis. Gene replacement and complementation experiments confirmed that phosphoribosylamidotransferase is essential for Conidiation of C. minitans. Mutant ZS-1T21882 did not grow on modified Czapek-Dox broth (MCD), but it grew well on MCD amended with IMP or AMP. The conidial production of this mutant was dependent on the dosage of IMP amended. At low concentrations, such as 0.1 mM and 0.25 mM, the mutant produced very few pycnidia, while up to 0.75 mM or higher, the Conidiation of this mutant was restored completely. cAMP could not restore the Conidiation of mutant ZS-1T21882 when amended into MCD, but could when amended into PDA. Neither GMP nor cGMP could restore the Conidiation in MCD or in PDA. Our findings suggest that phosphoribosylamidotransferase is essential for Conidiation of C. minitans via adenosine related molecules. Furthermore, when dual cultured with its host, this mutant produced conidia in the host mycelium and on the sclerotia of S. sclerotiorum, but not in dead mycelium or on dead sclerotia, suggesting that C. minitans is likely to able to obtain adenosine or related components from its host during parasitization.

Xianyun Sun - One of the best experts on this subject based on the ideXlab platform.

  • Table_1_The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa.XLS
    2019
    Co-Authors: Xianyun Sun, Bo Liu, Fei Wang, Nan Lan, Wei Xue, Zhenying Zhang
    Abstract:

    Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to Conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in Conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δada-6 mutant and wild type during Conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several Conidiation related genes are positively regulated by ADA-6, including genes that positively regulate Conidiation (fluffy and acon-3), and genes preferentially expressed during conidial development (eas, con-6, con-8, con-10, con-13, pcp-1, and NCU9357), as the expression of these genes were lower in the Δada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2, poi-2, and NCU05832, three key genes participating in sexual development. In both Conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H2O2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in Conidiation, sexual development, and oxidative stress response of N. crassa.

  • The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa
    Frontiers Media S.A., 2019
    Co-Authors: Xianyun Sun, Bo Liu, Fei Wang, Nan Lan, Wei Xue
    Abstract:

    Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to Conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in Conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δada-6 mutant and wild type during Conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several Conidiation related genes are positively regulated by ADA-6, including genes that positively regulate Conidiation (fluffy and acon-3), and genes preferentially expressed during conidial development (eas, con-6, con-8, con-10, con-13, pcp-1, and NCU9357), as the expression of these genes were lower in the Δada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2, poi-2, and NCU05832, three key genes participating in sexual development. In both Conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H2O2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in Conidiation, sexual development, and oxidative stress response of N. crassa

  • Analysis of the role of transcription factor VAD-5 in Conidiation of Neurospora crassa
    Fungal genetics and biology : FG & B, 2012
    Co-Authors: Xianyun Sun, Hanxing Zhang, Nan Lan, Shiping Wei, Xinyu Zhang
    Abstract:

    Conidiation is the major mode of reproduction in many filamentous fungi. The Neurospora crassa gene vad-5, which encodes a GAL4-like Zn2Cys6 transcription factor, was suggested to contribute to Conidiation in a previous study using a knockout mutant. In this study, we confirmed the positive contribution of vad-5 to Conidiation by gene complementation. To understand the role of vad-5 in Conidiation, transcriptomic profiles generated by digital gene expression profiling from the vad-5 deletion mutant and the wild-type strain were compared. Among 7559 detected genes, 176 genes were found to be transcriptionally down-regulated and 277 genes transcriptionally upregulated in the vad-5 deletion mutant, using ≥1-fold change as a cutoff threshold. Among the down-regulated genes, four which were already known to be involved in Conidiation -fluffy, ada-6, rca-1, and eas - were examined further in a time course experiment. Transcription of each of the four genes in the vad-5 deletion mutant was lower than in the wild-type strain during conidial development. Phenotypic observation of deletion mutants for 132 genes down-regulated by vad-5 deletion revealed that deletion mutants for 17 genes, including fluffy, ada-6, and eas, produced fewer conidia than the wild type. By phenotypic observation of deletion mutants for 211 genes upregulated in the vad-5 deletion mutant, two types of deletion mutants were found. One type, which produced more conidia than the wild-type strain, includes deletion mutants for previously characterized genes cat-2, cat-3, and sah-1 and for a non-characterized gene NCU07221. Deletion mutants of NCU06302 and NCU11090, representing the second type, produced conidia earlier than the wild-type strain. Based on these Conidiation phenotypes, we designated NCU07221 as high conidial production-1 (hcp-1) and named NCU06302 and NCU11090 as early conidial development-1 (ecd-1) and ecd-2, respectively. Given the collective results from this study, we propose that vad-5 exerts an effect on Conidiation by activating genes that positively contribute to Conidiation as well as by repressing genes that negatively influence conidial development.

  • involvement of a helix loop helix transcription factor chc 1 in co2 mediated Conidiation suppression in neurospora crassa
    Fungal Genetics and Biology, 2011
    Co-Authors: Xianyun Sun, Hanxing Zhang, Zhenying Zhang, Yong Wang
    Abstract:

    The morphological switch from vegetative growth to Conidiation in filamentous fungi is highly regulated, but the understanding of the regulatory mechanisms is limited. In this study, by screening a set of knock-out mutants corresponding to 103 transcription factor encoding genes in Neurospora crassa, a mutant was found to produce abundant conidia in race tubes in which Conidiation in the wild-type strain was suppressed. The corresponding gene NCU00749 encodes a protein containing a helix-loop-helix DNA binding region. Unlike enhanced Conidiation in ras-1 and sod-1 mutants, which was completely suppressed by antioxidant N-acetyl cysteine, enhanced Conidiation in the NCU00749 mutant was only slightly affected by N-acetyl cysteine. When grown on slants, the NCU00749 deletion mutant exhibited earlier conidial formation than the wild-type strain, and this was more evident at a higher (5%) CO(2) concentration. Therefore, we named NCU00749 as Conidiation at high carbon dioxide-1 (chc-1). Genes that are highly expressed during conidial development, eas, con-6, con-8 and con-10, were transcribed at a higher rate in the chc-1 deletion mutant than the wild-type strain in response to Conidiation induction. To determine the mechanisms by which CHC-1 regulates Conidiation, we conducted a RNA sequencing analysis and found that 404 genes exhibited ≥ 2 fold changes in transcription in response to chc-1 deletion. Among them, fluffy and ada-6, two transcription factor genes that positively regulate Conidiation in N. crassa, and rca-1, whose homolog flbD in Aspergillus nidulans is essential for Conidiation, were upregulated in the chc-1 deletion mutant. Results of RNA sequencing also suggest that signal transduction via the cAMP and the MAK-2 mediated signal pathways, and ROS generation and removal, mechanisms known to regulate Conidiation, are not involved in chc-1 mediated control of Conidiation. In addition, chc-1 also influences expression of genes involved in other important biological processes besides Conidiation such as carbon metabolism, sphingolipid synthesis, cell wall synthesis, and calcium signaling.

  • Involvement of a helix–loop–helix transcription factor CHC-1 in CO2-mediated Conidiation suppression in Neurospora crassa
    Fungal genetics and biology : FG & B, 2011
    Co-Authors: Xianyun Sun, Hanxing Zhang, Zhenying Zhang, Yong Wang
    Abstract:

    The morphological switch from vegetative growth to Conidiation in filamentous fungi is highly regulated, but the understanding of the regulatory mechanisms is limited. In this study, by screening a set of knock-out mutants corresponding to 103 transcription factor encoding genes in Neurospora crassa, a mutant was found to produce abundant conidia in race tubes in which Conidiation in the wild-type strain was suppressed. The corresponding gene NCU00749 encodes a protein containing a helix-loop-helix DNA binding region. Unlike enhanced Conidiation in ras-1 and sod-1 mutants, which was completely suppressed by antioxidant N-acetyl cysteine, enhanced Conidiation in the NCU00749 mutant was only slightly affected by N-acetyl cysteine. When grown on slants, the NCU00749 deletion mutant exhibited earlier conidial formation than the wild-type strain, and this was more evident at a higher (5%) CO(2) concentration. Therefore, we named NCU00749 as Conidiation at high carbon dioxide-1 (chc-1). Genes that are highly expressed during conidial development, eas, con-6, con-8 and con-10, were transcribed at a higher rate in the chc-1 deletion mutant than the wild-type strain in response to Conidiation induction. To determine the mechanisms by which CHC-1 regulates Conidiation, we conducted a RNA sequencing analysis and found that 404 genes exhibited ≥ 2 fold changes in transcription in response to chc-1 deletion. Among them, fluffy and ada-6, two transcription factor genes that positively regulate Conidiation in N. crassa, and rca-1, whose homolog flbD in Aspergillus nidulans is essential for Conidiation, were upregulated in the chc-1 deletion mutant. Results of RNA sequencing also suggest that signal transduction via the cAMP and the MAK-2 mediated signal pathways, and ROS generation and removal, mechanisms known to regulate Conidiation, are not involved in chc-1 mediated control of Conidiation. In addition, chc-1 also influences expression of genes involved in other important biological processes besides Conidiation such as carbon metabolism, sphingolipid synthesis, cell wall synthesis, and calcium signaling.

Fei Wang - One of the best experts on this subject based on the ideXlab platform.

  • Development of rice Conidiation media for Ustilaginoidea virens
    PloS one, 2019
    Co-Authors: Yufu Wang, Junbin Huang, Fei Wang, Songlin Xie, Yi Liu, Weixiao Yin, Chaoxi Luo
    Abstract:

    Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate Conidiation of U. virens. Among the tested media, 0.10 g/ml panicle medium was most efficient for Conidiation. Whereas, some rice leaf media more effectively increased Conidiation than panicle media except 0.10 g/ml panicle medium, and certain non-filtered tissue media were better than their filtered counterparts. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. The rice tissue medium is also workable in inducing conidia for Conidiation-defective isolates. This method provides a foundation for the production of conidia by U. virens that will be widely applicable in pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

  • Development of rice Conidiation media for Ustilaginoidea virens
    2019
    Co-Authors: Yufu Wang, Junbin Huang, Fei Wang, Songlin Xie, Yi Liu, Weixiao Yin, Chaoxi Luo
    Abstract:

    Abstract Rice false smut, caused by the ascomycete Ustilaginoidea virens, is a serious disease of rice worldwide. Conidia are very important infectious propagules of U. virens, but the ability of pathogenic isolates to produce conidia frequently decreases in culture, which influences pathogenicity testing. Here, we developed tissue media with rice leaves or panicles that stimulate Conidiation of U. virens. Generally, rice leaf media more effectively increased Conidiation than panicle media, and certain non-filtered tissue media were better than their filtered counterparts. Among the tested media, the Indica rice leaf medium with 0.06 g/ml of Wanxian 98 leaf was most efficient for inducing Conidiation, and it was also usable for Conidiation-defective isolates. Although the conidia induced in rice tissue media were smaller, they were able to germinate on potato sucrose agar medium and infect rice normally. This method provides a foundation for the production of conidia in U. virens that will be widely applied in the pathogenicity testing as well as in genetic analyses for false smut resistance in rice cultivars.

  • Table_1_The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa.XLS
    2019
    Co-Authors: Xianyun Sun, Bo Liu, Fei Wang, Nan Lan, Wei Xue, Zhenying Zhang
    Abstract:

    Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to Conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in Conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δada-6 mutant and wild type during Conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several Conidiation related genes are positively regulated by ADA-6, including genes that positively regulate Conidiation (fluffy and acon-3), and genes preferentially expressed during conidial development (eas, con-6, con-8, con-10, con-13, pcp-1, and NCU9357), as the expression of these genes were lower in the Δada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2, poi-2, and NCU05832, three key genes participating in sexual development. In both Conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H2O2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in Conidiation, sexual development, and oxidative stress response of N. crassa.

  • The Zn(II)2Cys6-Type Transcription Factor ADA-6 Regulates Conidiation, Sexual Development, and Oxidative Stress Response in Neurospora crassa
    Frontiers Media S.A., 2019
    Co-Authors: Xianyun Sun, Bo Liu, Fei Wang, Nan Lan, Wei Xue
    Abstract:

    Conidiation and sexual development are critical for reproduction, dispersal and better-adapted survival in many filamentous fungi. The Neurospora crassa gene ada-6 encodes a Zn(II)2Cys6-type transcription factor, whose deletion resulted in reduced conidial production and female sterility. In this study, we confirmed the positive contribution of ada-6 to Conidiation and sexual development by detailed phenotypic characterization of its deletion mutant and the complemented mutant. To understand the regulatory mechanisms of ADA-6 in Conidiation and sexual development, transcriptomic profiles generated by RNA-seq from the Δada-6 mutant and wild type during Conidiation and sexual development were compared. During conidial development, differential expressed genes (DEGs) between the Δada-6 mutant and wild type are mainly involved in oxidation-reduction process and single-organism metabolic process. Several Conidiation related genes are positively regulated by ADA-6, including genes that positively regulate Conidiation (fluffy and acon-3), and genes preferentially expressed during conidial development (eas, con-6, con-8, con-10, con-13, pcp-1, and NCU9357), as the expression of these genes were lower in the Δada-6 mutant compared to wild type during conidial development. Phenotypic observation of deletion mutants for other genes with unknown function down-regulated by ada-6 deletion revealed that deletion mutants for four genes (NCU00929, NCU05260, NCU00116, and NCU04813) produced less conidia than wild type. Deletion of ada-6 resulted in female sterility, which might be due to that ADA-6 affects oxidation-reduction process and transmembrane transport process, and positively regulates the transcription of pre-2, poi-2, and NCU05832, three key genes participating in sexual development. In both Conidiation and the sexual development process, ADA-6 regulates the transcription of cat-3 and other genes participating in reactive oxygen species production according to RNA-seq data, indicating a role of ADA-6 in oxidative stress response. This was further confirmed by the results that deletion of ada-6 led to hypersensitivity to oxidants H2O2 and menadione. Together, these results proved that ADA-6, as a global regulator, plays a crucial role in Conidiation, sexual development, and oxidative stress response of N. crassa