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Mahua Ghosh - One of the best experts on this subject based on the ideXlab platform.
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protective effect of Conjugated Linolenic Acid isomers present in vegetable oils against arsenite induced renal toxicity in rat model
Nutrition, 2013Co-Authors: Siddhartha Sankar Saha, Mahua GhoshAbstract:Abstract Objective The aim of the present study was to investigate the protective effect of Conjugated Linolenic Acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress. Methods Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid, respectively, along with Sa by oral gavage once daily. Results Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly ( P P Conclusion CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity.
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Conjugated Linolenic Acid nanoparticles inhibit hypercholesterolemia induced by feeding a high-fat diet in male albino rats
Journal of Food Science and Technology, 2013Co-Authors: Avery Sengupta, Surashree Sen Gupta, Isita Nandi, Mahua GhoshAbstract:Conjugated Linolenic Acid (CLnA) refers to a group of octadecatrienoic Acid isomers that have three double bonds in conjugation. However, CLnA is highly prone to oxidative damage due to the presence of unsaturation in CLnA. Nanoencapsulation offers increased stability of the CLnA molecule so that CLnA can show its full activity. The present study was designed to clarify the effects of two types of CLnA rich oil nanocapsules (CLnA rich oil nanocapsules with lipoic Acid and CLnA rich oil nanocapsules with tert-butylhydroquinone [TBHQ]) on hypercholesterolemia induced by feeding a high-fat diet in male albino rats. CLnA rich oil nanocapsules were prepared by the ultrasonication method and the freeze-drying technique. The mean size of CLnA rich oil nanocapsules varied from 150 to 160 nm. The feeding experiment was carried out for a period of 30 days. Results showed that CLnA rich oil nanocapsules reduced the blood lipids, tissue lipids and plasma viscosity significantly. This paper is the first report of the lipid-lowering effects of CLnA rich oil nanocapsules suggesting that the CLnA rich oil nanocapsules could be used for the treatment of hypercholesterolemia. Lipoic Acid showed better protective effect than TBHQ.
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Protective effect of Conjugated Linolenic Acid isomers present in vegetable oils against arsenite-induced renal toxicity in rat model
Nutrition, 2013Co-Authors: S S Saha, Mahua GhoshAbstract:OBJECTIVE: The aim of the present study was to investigate the protective effect of Conjugated Linolenic Acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress. METHODS: Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid, respectively, along with Sa by oral gavage once daily. RESULTS: Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly (P < 0.05) in arsenite-treated rats compared with control. Fatty-Acid composition of renal lipids showed significant decrease in monounsaturated fatty Acid, polyunsaturated fatty Acid (PUFA) content, and increase in saturated fatty Acid content due to oxidative stress. PUFA such as γ-Linolenic Acid, eicosapentaenoic Acid, and docosahexaenoic Acid decreased significantly with significant (P < 0.05) increase in arachidonic Acid content after Sa treatment. Administration of blended product of both the isomers caused better restoration of renal fatty Acids and other altered parameters. CONCLUSION: CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity.
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Protective effect of Conjugated Linolenic Acid isomers present in vegetable oils against arsenite-induced renal toxicity in rat model
Nutrition (Burbank Los Angeles County Calif.), 2013Co-Authors: Siddhartha Sankar Saha, Mahua GhoshAbstract:The aim of the present study was to investigate the protective effect of Conjugated Linolenic Acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress. Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid, respectively, along with Sa by oral gavage once daily. Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly (P < 0.05) in arsenite-treated rats compared with control. Fatty-Acid composition of renal lipids showed significant decrease in monounsaturated fatty Acid, polyunsaturated fatty Acid (PUFA) content, and increase in saturated fatty Acid content due to oxidative stress. PUFA such as γ-Linolenic Acid, eicosapentaenoic Acid, and docosahexaenoic Acid decreased significantly with significant (P < 0.05) increase in arachidonic Acid content after Sa treatment. Administration of blended product of both the isomers caused better restoration of renal fatty Acids and other altered parameters. CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity. Copyright © 2013 Elsevier Inc. All rights reserved.
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Microencapsulation of Conjugated Linolenic Acid-rich pomegranate seed oil by an emulsion method
Food science and technology international = Ciencia y tecnologia de los alimentos internacional, 2012Co-Authors: Surashree Sen Gupta, Santinath Ghosh, P.k. Maiti, Mahua GhoshAbstract:Controlled release of food ingredients and their protection from oxidation are the key functionality provided by microencapsulation. In the present study, pomegranate seed oil, rich in Conjugated Linolenic Acid, was microencapsulated. As encapsulating agent, sodium alginate or trehalose was used. Calcium caseinate was used as the emulsifier. Performances of the two encapsulants were compared in respect of the rate of release of core material from the microcapsules and stability of microcapsules against harsh conditions. Microencapsulation was carried out by preparation of an emulsion containing calcium caseinate as the emulsion stabilizer and a water-soluble carbohydrate (either sodium alginate or trehalose) as the encapsulant. An oil-in-water emulsion was prepared with pomegranate seed oil as the inner core material. The emulsion was thereby freeze-dried and the dried product pulverized. External morphology of the microcapsules was studied under scanning electron microscope. Micrographs showed that both types of microcapsules had uneven surface morphology. Release rate of the microcapsules was studied using UV-spectrophotometer. Trehalose-based microcapsules showed higher release rate. On subjecting the microcapsules at 110 � C for specific time periods, it was observed that sodium alginate microcapsules retained their original properties. Hence, we can say that sodium alginate microcapsules are more heat resistant than trehalose microcapsules.
Gaofeng Yuan - One of the best experts on this subject based on the ideXlab platform.
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Modulation of peroxisome proliferator-activated receptor gamma (PPAR γ) by Conjugated fatty Acid in obesity and inflammatory bowel disease.
Journal of Agricultural and Food Chemistry, 2015Co-Authors: Gaofeng Yuan, Xiaoe Chen, Duo LiAbstract:Conjugated fatty Acids including Conjugated linoleic Acid (CLA) and Conjugated Linolenic Acid (CLNA) have drawn significant attention for their variety of biologically beneficial effects. Evidence suggested that CLA and CLNA could play physiological roles by regulating the expression and activity of PPAR γ. This review summarizes the current understanding of evidence of the role of CLA (cis-9,trans-11 CLA and trans-10,cis-12 CLA) and CLNA (punicic Acid and α-eleostearic Acid) in modulating the expression or activity of PPAR γ that could in turn be employed as complementary treatment for obesity and inflammatory bowel disease.
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Conjugated Linolenic Acids and their bioactivities: a review.
Food & function, 2014Co-Authors: Gaofeng Yuan, Xiaoe ChenAbstract:Conjugated Linolenic Acid (CLNA) is a mixture of positional and geometric isomers of octadecatrienoic Acid (α-Linolenic Acid, cis9,cis12,cis15-18:3 n-3) found in plant seeds. Three 8,10,12-18:3 isomers and four 9,11,13-18:3 isomers have been reported to occur naturally. CLNA isomers such as punicic Acid, α-eleostearic Acid and jacaric Acid have been attributed to exhibit several health benefits that are largely based on animal and in vitro studies. This review has summarized and updated the evidence regarding the metabolism and bioactivities of CLNA isomers, and comprehensively discussed the recent studies on the effects of anti-carcinogenic, lipid metabolism regulation, anti-inflammatory, anti-obese and antioxidant activities of CLNA isomers. The available results may provide a potential application for CLNA isomers from natural sources, especially edible plant seeds, as effective functional food ingredients and dietary supplements for the above mentioned disease management. Further research, especially human randomized clinical trials, is warranted to investigate the detailed physiological effects, bioactivity and molecular mechanism of CLNA.
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Effects of Geographical Origin on the Conjugated Linolenic Acid of Trichosanthes kirilowii Maxim Seed Oil
Journal of the American Oil Chemists' Society, 2011Co-Authors: Jing Yang, Gaofeng Yuan, Ci Qin Zhou, Duo LiAbstract:Trichosanthes kirilowii Maxim (T. kirilowii) seeds from four geographical locations (Changxing, Quzhou, Yuexi, Dongzhi) contained 26.15–49.41% oil and 28.68–37.90% protein. The seed oil was distinguished by the Conjugated Linolenic Acids, punicic Acid (PA) and α-eleostearic Acid (α-ESA). The main fatty Acids in T. kirilowii seed oils were ranked in the following order: punicic Acid (33.09–39.15%), Linolenic Acid (33.77–38.66%), oleic Acid (15.15–24.88%), palmitic Acid (2.36–4.86%). PA was the main isomer of CLNA (33.09–39.15%). No significant differences were found either in PA content or in α-ESA content of T. kirilowii seed from these geographical locations. Little difference was observed in the quantitative composition of the lipid contents of seeds from different geographical locations. The α-tocopherol content of T. kirilowii seed ranged from 6.34 to 31.74 mg/100 g, with the highest levels in Changxing seeds. The present results showed that T. kirilowii seeds were especially rich in PA, and their contents were not influenced by the geographical locations. Variation in some proximate compositions by geographical locations may be caused by ecological conditions, temperature, climate condition, technical and cultural conditions.
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punicic Acid from trichosanthes kirilowii seed oil is rapidly metabolized to Conjugated linoleic Acid in rats
Journal of Medicinal Food, 2009Co-Authors: Gaofeng Yuan, Jingqun Yuan, Duo LiAbstract:ABSTRACT The incorporation and metabolism of orally administered punicic Acid (PA), one isomer of Conjugated Linolenic Acid (CLNA), in rat tissues and plasma were studied over a 24-hour period. The punic Acid was derived from Trichosanthes kirilowii Maxim seed oil, a unique PA-containing material, and identified and analyzed by high-performance liquid chromatography and gas chromatography-mass spectrometry. The results show that PA was incorporated and metabolized to 9c,11t-Conjugated linoleic Acid (CLA) in rat plasma, liver, kidney, heart, brain, and adipose tissue. The level of PA and CLA in liver and plasma was higher than in brain, heart, kidney, and adipose tissue, and the lowest accumulation occurred in the brain. The observation that PA can be converted into 9c,11t-CLA has gained increased importance since it has been demonstrated that 9c,11t-CLA exerts many biological activities. Therefore natural resources containing CLNA, especially edible T. kirilowii seed, could be a potential dietary source o...
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Effects of Conjugated Linolenic Acid and Conjugated linoleic Acid on lipid metabolism in mice.
European Journal of Lipid Science and Technology, 2009Co-Authors: Gaofeng Yuan, Haiyan Sun, Andrew J. SinclairAbstract:The effects of two isomers of Conjugated Linolenic Acid (CLnA), α-eleostearic Acid (α-ESA) and punicic Acid (PA), on body fat and lipid metabolism were investigated, compared with a Conjugated linoleic Acid (CLA) mixture (primarily cis9,trans11- and trans10,cis12-18:2) and α-Linolenic Acid (ALA), a non-Conjugated octadecatrienoic Acid, in the present study. ICR mice were fed either a control diet or one of four experimental diets supplemented with 1 % α-ESA, 1 % PA, 1 % CLA mixture and 1 % ALA in the form of triacylglycerols (TAG) for 6 weeks. The weights of perirenal and epididymal adipose tissues were significantly decreased while the liver weight was significantly increased in mice fed CLA, compared with the control. In contrast to CLA, the tissue weights in α―ESA-, PA- and ALA-fed mice were not affected. No significant differences were observed in TAG, total cholesterol, high-density lipoprotein and low-density lipoprotein cholesterol levels among the five groups. The liver TAG level was significantly decreased in mice fed α-ESA and PA while it was significantly increased in mice fed the CLA mixture. These results indicate that CLnA and CLA have differential effects on body fat mass and liver TAG levels in mice.
Yi-chun Chou - One of the best experts on this subject based on the ideXlab platform.
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cis 9 trans 11 trans 13 Conjugated Linolenic Acid induces apoptosis and sustained erk phosphorylation in 3t3 l1 preadipocytes
Nutrition, 2012Co-Authors: Yi-chun Chou, Tzu-wen Lai, Jong-ho Chyuan, Pei-min ChaoAbstract:Abstract Objective The aim of this study was to investigate the adipogenic effect of cis -9, trans -11, trans -13-Conjugated Linolenic Acid ( c 9, t 11, t 13 - CLN), a fatty Acid naturally present in bitter melon. Methods The 3T3-L1 murine preadipocyte cell line was used to test the effect of saponifiables from whole bitter melon and of commercially prepared pure c 9 ,t 11 ,t 13 - CLN on adipocyte differentiation. The effect of c 9 ,t 11 ,t 13 - CLN on 3T3-L1 cell viability was also tested at proliferation, mitotic clonal expansion, and terminal differentiation stages. Results Compared to the free fatty Acid control mixture, the proadipogenic effect on 3T3-L1 was less potent using saponifiables obtained from bitter melon. C 9 ,t 11 ,t 13 - CLN, unlike its non-Conjugated counterpart Linolenic Acid (LN) or other common fatty Acids such as oleic Acid or linoleic Acid, exerted no proadipogenic effect on 3T3-L1. In contrast to LN displaying no cytotoxic effect at a concentration ≤100 μM, c 9 ,t 11 ,t 13 - CLN caused a dose-dependent reduction in the viability of pre- and postconfluent preadipocytes associated with apoptosis. Sustained ERK/MAPK activation, accompanied by increased peroxisome proliferator-activated receptor γ phosphorylation, was seen in c 9 ,t 11 ,t 13 - CLN-treated cells at initiation of differentiation. Conclusion C9 ,t 11 ,t 13 - CLN is less adipogenic for 3T3-L1 cells than LN and this is partly due to its apoptotic effect on proliferating preadipocytes and to the sustained ERK phosphorylation seen during mitotic clonal expansion.
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cis-9, trans-11, trans-13-Conjugated Linolenic Acid induces apoptosis and sustained ERK phosphorylation in 3T3-L1 preadipocytes.
Nutrition (Burbank Los Angeles County Calif.), 2012Co-Authors: Yi-chun Chou, Tzu-wen Lai, Jong-ho Chyuan, Pei-min ChaoAbstract:The aim of this study was to investigate the adipogenic effect of cis-9, trans-11, trans-13-Conjugated Linolenic Acid (c9,t11,t13-CLN), a fatty Acid naturally present in bitter melon. The 3T3-L1 murine preadipocyte cell line was used to test the effect of saponifiables from whole bitter melon and of commercially prepared pure c9,t11,t13-CLN on adipocyte differentiation. The effect of c9,t11,t13-CLN on 3T3-L1 cell viability was also tested at proliferation, mitotic clonal expansion, and terminal differentiation stages. Compared to the free fatty Acid control mixture, the proadipogenic effect on 3T3-L1 was less potent using saponifiables obtained from bitter melon. C9,t11,t13-CLN, unlike its non-Conjugated counterpart Linolenic Acid (LN) or other common fatty Acids such as oleic Acid or linoleic Acid, exerted no proadipogenic effect on 3T3-L1. In contrast to LN displaying no cytotoxic effect at a concentration ≤100 μM, c9,t11,t13-CLN caused a dose-dependent reduction in the viability of pre- and postconfluent preadipocytes associated with apoptosis. Sustained ERK/MAPK activation, accompanied by increased peroxisome proliferator-activated receptor γ phosphorylation, was seen in c9,t11,t13-CLN-treated cells at initiation of differentiation. C9,t11,t13-CLN is less adipogenic for 3T3-L1 cells than LN and this is partly due to its apoptotic effect on proliferating preadipocytes and to the sustained ERK phosphorylation seen during mitotic clonal expansion. Copyright © 2012 Elsevier Inc. All rights reserved.
S S Saha - One of the best experts on this subject based on the ideXlab platform.
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Protective effect of Conjugated Linolenic Acid isomers present in vegetable oils against arsenite-induced renal toxicity in rat model
Nutrition, 2013Co-Authors: S S Saha, Mahua GhoshAbstract:OBJECTIVE: The aim of the present study was to investigate the protective effect of Conjugated Linolenic Acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress. METHODS: Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid, respectively, along with Sa by oral gavage once daily. RESULTS: Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly (P < 0.05) in arsenite-treated rats compared with control. Fatty-Acid composition of renal lipids showed significant decrease in monounsaturated fatty Acid, polyunsaturated fatty Acid (PUFA) content, and increase in saturated fatty Acid content due to oxidative stress. PUFA such as γ-Linolenic Acid, eicosapentaenoic Acid, and docosahexaenoic Acid decreased significantly with significant (P < 0.05) increase in arachidonic Acid content after Sa treatment. Administration of blended product of both the isomers caused better restoration of renal fatty Acids and other altered parameters. CONCLUSION: CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity.
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Synergistic effect of Conjugated Linolenic Acid isomers against induced oxidative stress, inflammation and erythrocyte membrane disintegrity in rat model
Biochimica et Biophysica Acta, 2012Co-Authors: S S Saha, Pritha Dasgupta, Sumita Sengupta, Mahua GhoshAbstract:Abstract Background α-Eleostearic Acid and punicic Acid, two typical Conjugated Linolenic Acid (CLnA) isomers present in bitter gourd and snake gourd oil respectively, exhibit contrasting cis-trans configuration which made them biologically important. Methods Rats were divided into six groups. Group 1 was control and group 2 was treated control. Rats in the groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0% respectively) while rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid respectively along with sodium arsenite by oral gavage once per day. Results Results showed that increase in nitric oxide synthase (NOS) activity, inflammatory markers expression, platelet aggregation, lipid peroxidation, protein oxidation, DNA damage and altered expression of liver X receptor-α (LXR-α) after arsenite treatment were restored with the supplementation of oils containing CLnA isomers. Altered activities of different antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase and ferric reducing ability of plasma (FRAP) also restored after oil supplementation. Altered morphology and fluidity of erythrocyte membrane studied by atomic force and scanning electron microscopy, after stress induction were significantly improved due to amelioration in cholesterol/phospholipid ratio and fatty Acid profile of membrane. Oils treatment also improved morphology of liver and fatty Acid composition of hepatic lipid. Conclusions Overall two isomers showed synergistic antioxidant and anti-inflammatory effect against induced perturbations and membrane disintegrity. General significance Synergistic antioxidant and anti-inflammatory role of these CLnA isomers were established by this study.
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Antioxidant and anti-inflammatory effect of Conjugated Linolenic Acid isomers against streptozotocin-induced diabetes.
British Journal of Nutrition, 2011Co-Authors: S S Saha, Mahua GhoshAbstract:The present study was undertaken to evaluate the effect of α-eleostearic Acid and punicic Acid, two isomers of Conjugated Linolenic Acid (CLnA) present in bitter gourd ( Momordica charantia ) and snake gourd oil ( Trichosanthes anguina ), respectively, against oxidative stress, inflammatory challenge and aberration in erythrocyte morphology due to streptozotocin (STZ)-induced diabetes. Male albino rats were divided into four groups consisting of eight animals in each group. The first group served as control and diabetes was induced in rats in groups 2–4 by a single intraperitoneal injection of STZ. Moreover, rats in groups 3 and 4 were treated with 0·5 % of α-eleostearic Acid and 0·5 % of punicic Acid of the total lipid given, respectively, by oral administration once per d. After administration, CLnA isomers had significantly reduced oxidative stress, lipid peroxidation and restored antioxidant and pro-inflammatory enzymes such as superoxide dismutase, catalase, and glutathione peroxidase, reduced glutathione, NO synthase level in pancreas, blood and erythrocyte lysate. The ferric reducing antioxidant power (FRAP) assay of plasma showed that CLnA treatment caused improvement in the FRAP value which was altered after STZ treatment due to an increased level of free radicals. Expression of inflammatory cytokines such as TNF-α and IL-6 in blood and expression of hepatic NF-κB (p65) increased significantly after STZ treatment due to increased inflammation which was restored with the administration of CLnA isomers. From the obtained results, it could be concluded that α-eleostearic Acid and punicic Acid showed potent antioxidant and anti-inflammatory activity with varying effectivity.
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Antioxidant effect of vegetable oils containing Conjugated Linolenic Acid isomers against induced tissue lipid peroxidation and inflammation in rat model.
Chemico-biological interactions, 2011Co-Authors: S S Saha, Mahua GhoshAbstract:The purpose of the present study was to examine the antioxidant activity of two typical oils obtained from two vegetables, bitter gourd seed and snake gourd seed, containing two different isomers of Conjugated Linolenic Acid (CLnA) against oxidative stress induced by sodium arsenite in relation to tissue lipid peroxidation and inflammation. Male albino rats were taken as subject and divided into six groups: Group 1 was control and Group 2 was treated with sodium arsenite (Sa; 10mg/Kg BW); Groups 3-6 were orally treated with different doses of seed oils maintaining definite concentration of CLnA isomers (0.5% and 1.0% of total lipid for each CLnA isomer) along with sodium arsenite. There was significant increase in lipid peroxidation, pro-oxidant enzyme activity and decrease in antioxidant enzyme activity in brain due to Sa administration. Decrease in total protein content was also observed in plasma, liver and brain of Sa treated group. Significant decrease in phospholipid content and increase in total lipid content and cholesterol content were observed in arsenite treated group. There was significant increase in relative organ weight of liver due to Sa administration. Fatty Acid profile of liver and brain lipid shows significant (P
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Ameliorative role of Conjugated Linolenic Acid isomers against oxidative DNA damage induced by sodium arsenite in rat model.
Food and Chemical Toxicology, 2010Co-Authors: S S Saha, Mahua GhoshAbstract:Abstract The present study was undertaken to evaluate the ameliorative role of α-eleostearic Acid and punicic Acid, isomers of Conjugated Linolenic (CLnA) Acid, against oxidative stress induced DNA damage. Male albino rats were divided into six groups. Group 1 and 2 were normal control and sodium arsenite treated (Sa; 10 mg/kg BW) control respectively. Group 3–6 were orally treated with different doses of two fatty Acids (0.5% and 1.0% of total lipid given for each isomer) along with sodium arsenite (Sa; 10 mg/kg BW). Comet assay of blood leukocytes showed that administration of CLnA reduced DNA damage significantly ( P
Siddhartha Sankar Saha - One of the best experts on this subject based on the ideXlab platform.
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protective effect of Conjugated Linolenic Acid isomers present in vegetable oils against arsenite induced renal toxicity in rat model
Nutrition, 2013Co-Authors: Siddhartha Sankar Saha, Mahua GhoshAbstract:Abstract Objective The aim of the present study was to investigate the protective effect of Conjugated Linolenic Acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress. Methods Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid, respectively, along with Sa by oral gavage once daily. Results Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly ( P P Conclusion CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity.
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Protective effect of Conjugated Linolenic Acid isomers present in vegetable oils against arsenite-induced renal toxicity in rat model
Nutrition (Burbank Los Angeles County Calif.), 2013Co-Authors: Siddhartha Sankar Saha, Mahua GhoshAbstract:The aim of the present study was to investigate the protective effect of Conjugated Linolenic Acid (CLnA), present in vegetable oils against arsenite-induced renal oxidative stress. Albino rats were divided into six groups. Group 1 was control and group 2 was treated with sodium arsenite (Sa; 10 mg/kg BW). Rats in groups 3 and 4 were treated with mixture of α-eleostearic Acid and punicic Acid (1:1) (0.5% and 1.0%, respectively), whereas rats in the groups 5 and 6 were treated with 0.5% of α-eleostearic Acid and 0.5% of punicic Acid, respectively, along with Sa by oral gavage once daily. Results revealed that activity of antioxidant enzymes and total reduced glutathione content, total protein content, and phospholipid content in kidney were decreased significantly in arsenite-treated group compared with control. Activity of nitric oxide synthase, peroxidation of lipid, protein oxidation, total cholesterol content, total lipid content of kidney, and plasma creatinine level were increased significantly (P < 0.05) in arsenite-treated rats compared with control. Fatty-Acid composition of renal lipids showed significant decrease in monounsaturated fatty Acid, polyunsaturated fatty Acid (PUFA) content, and increase in saturated fatty Acid content due to oxidative stress. PUFA such as γ-Linolenic Acid, eicosapentaenoic Acid, and docosahexaenoic Acid decreased significantly with significant (P < 0.05) increase in arachidonic Acid content after Sa treatment. Administration of blended product of both the isomers caused better restoration of renal fatty Acids and other altered parameters. CLnA isomers caused amelioration of renal oxidative stress and the isomers showed synergistic activity. Copyright © 2013 Elsevier Inc. All rights reserved.
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In vitro antioxidant study of vegetable oils containing Conjugated Linolenic Acid isomers
LWT - Food Science and Technology, 2012Co-Authors: Siddhartha Sankar Saha, Maumita Patra, Mahua GhoshAbstract:Abstract The study aimed to evaluate the antioxidant activity ( in vitro ) of vegetable oils containing Conjugated Linolenic Acid (CLnA) isomers such as α-eleostearic and punicic Acid and also to evaluate the comparative effectiveness of these vegetable oils due to presence of cis – trans isomers in variable amount. Different in vitro methods were used to evaluate the free radical scavenging activity, hydroxyl radical scavenging activity, metal chelating activity and reducing activity of oils at different concentrations of CLnA isomers such as 125, 250 and 375 μg/mL. Inhibition on lipid peroxidation was evaluated by measuring thiobarbituric Acid responsive substance (TBARS) and Conjugated diene formation at 125 and 250 μg/mL concentrations of CLnA. Both the oils showed potent free radical scavenging activity at 375 μg/mL concentration. In contrary, these oils showed higher hydroxyl radical scavenging, metal chelation and reducing activity at lower concentration i.e. 125 μg/mL. TBARS formation and Conjugated diene formation was lower i.e. inhibition of lipid peroxidation was maximum at 125 μg/mL of both CLnA isomers. Overall, both the oils showed better antioxidant activity at lower concentration due to better oxidative stability and bitter gourd oil showed more prominent antioxidant activity than snake gourd oil due to presence of higher trans content.
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Comparative study of hypocholesterolemic and hypolipidemic effects of Conjugated Linolenic Acid isomers against induced biochemical perturbations and aberration in erythrocyte membrane fluidity.
European journal of nutrition, 2011Co-Authors: Siddhartha Sankar Saha, Anirban Chakraborty, Santinath Ghosh, Mahua GhoshAbstract:Aim of study The purpose of the study was to evaluate hypolipidemic and hypocholesterolemic activities of Conjugated Linolenic Acid (CLnA) isomers, present in bitter gourd and snake gourd seed, in terms of amelioration of plasma lipid profile, lipoprotein oxidation and erythrocyte membrane fluidity after oral administration.
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antioxidant effect of vegetable oils containing Conjugated Linolenic Acid isomers against induced tissue lipid peroxidation and inflammation in rat model
Chemico-Biological Interactions, 2011Co-Authors: Siddhartha Sankar Saha, Mahua GhoshAbstract:The purpose of the present study was to examine the antioxidant activity of two typical oils obtained from two vegetables, bitter gourd seed and snake gourd seed, containing two different isomers of Conjugated Linolenic Acid (CLnA) against oxidative stress induced by sodium arsenite in relation to tissue lipid peroxidation and inflammation. Male albino rats were taken as subject and divided into six groups: Group 1 was control and Group 2 was treated with sodium arsenite (Sa; 10mg/Kg BW); Groups 3-6 were orally treated with different doses of seed oils maintaining definite concentration of CLnA isomers (0.5% and 1.0% of total lipid for each CLnA isomer) along with sodium arsenite. There was significant increase in lipid peroxidation, pro-oxidant enzyme activity and decrease in antioxidant enzyme activity in brain due to Sa administration. Decrease in total protein content was also observed in plasma, liver and brain of Sa treated group. Significant decrease in phospholipid content and increase in total lipid content and cholesterol content were observed in arsenite treated group. There was significant increase in relative organ weight of liver due to Sa administration. Fatty Acid profile of liver and brain lipid shows significant (P<0.05) reduction in most of the polyunsaturated fatty Acids and increase in arachidonic Acid (20:4n-6) (75.23%) due to inflammation after arsenite treatment. Administration of experimental oils made almost complete restoration of those altered parameters. Overall, these two oils were effective in protecting tissue lipid profiles which were altered due to oxidative stress.