The Experts below are selected from a list of 1785 Experts worldwide ranked by ideXlab platform

Mingwu Wang - One of the best experts on this subject based on the ideXlab platform.

  • Expression patterns of Conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress.
    PloS one, 2017
    Co-Authors: Dhruva Bhattacharya, Mingwu Wang
    Abstract:

    The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the Conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse Conjunctiva Epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that Conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.

Dhruva Bhattacharya - One of the best experts on this subject based on the ideXlab platform.

  • Expression patterns of Conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress.
    PloS one, 2017
    Co-Authors: Dhruva Bhattacharya, Mingwu Wang
    Abstract:

    The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the Conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse Conjunctiva Epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that Conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.

Min Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Vascular endothelial growth factor and chemokine receptor-3 in the pathogenesis of pterygium
    Press of International Journal of Ophthalmology (IJO PRESS), 2019
    Co-Authors: Hui Liu, Min Zhang
    Abstract:

    AIM: To investigate the expression of vascular endothelial growth factor(VEGF)and chemokine receptor 3(CCR3)in pterygium.METHODS: The expression of VEGF and CCR3 were detected in 18 patients(18 eyes)with pterygium and compared with the normal Conjunctiva Epithelium in other 14 cases(14 eyes). The histological differences between the two groups were compared by hematoxylin and eosin(HE)staining. The expression of VEGF and CCR3 in pterygium and normal Conjunctival tissues were analyzed by immunofluorescence and immunohistochemical. The mRNA expression of VEGF and CCR3 were assessed by Real-time PCR.RESULTS: Compared with normal Conjunctival tissue, the epithelial layer of pterygium was obvious thickening, the stromal layer was more disordered, and the expression levels of VEGF(0.69±0.0875 vs 0.05±0.0024)and CCR3(0.45±0.0248 vs 0.03±0.0074)were significantly elevated(all P

  • Vascular endothelial growth factor and chemokine receptor-3 in the pathogenesis of pterygium
    Press of International Journal of Ophthalmology (IJO PRESS), 2019
    Co-Authors: Hui Liu, Min Zhang
    Abstract:

    AIM: To investigate the expression of vascular endothelial growth factor(VEGF)and chemokine receptor 3(CCR3)in pterygium.METHODS: The expression of VEGF and CCR3 were detected in 18 patients(18 eyes)with pterygium and compared with the normal Conjunctiva Epithelium in other 14 cases(14 eyes). The histological differences between the two groups were compared by hematoxylin and eosin(HE)staining. The expression of VEGF and CCR3 in pterygium and normal Conjunctival tissues were analyzed by immunofluorescence and immunohistochemical. The mRNA expression of VEGF and CCR3 were assessed by Real-time PCR.RESULTS: Compared with normal Conjunctival tissue, the epithelial layer of pterygium was obvious thickening, the stromal layer was more disordered, and the expression levels of VEGF(0.69±0.0875 vs 0.05±0.0024)and CCR3(0.45±0.0248 vs 0.03±0.0074)were significantly elevated(all Pvs 1.00±0.08), and the expression level of CCR3 mRNA was about 160 times as many as Conjunctival tissue(159.60±34.15 vs 1.00±0.09).CONCLUSION: The expression of VEGF and CCR3 in pterygium was significantly increased, suggesting that they may be involved in the occurrence of pterygium and promote the development of the disease

Hui Liu - One of the best experts on this subject based on the ideXlab platform.

  • Vascular endothelial growth factor and chemokine receptor-3 in the pathogenesis of pterygium
    Press of International Journal of Ophthalmology (IJO PRESS), 2019
    Co-Authors: Hui Liu, Min Zhang
    Abstract:

    AIM: To investigate the expression of vascular endothelial growth factor(VEGF)and chemokine receptor 3(CCR3)in pterygium.METHODS: The expression of VEGF and CCR3 were detected in 18 patients(18 eyes)with pterygium and compared with the normal Conjunctiva Epithelium in other 14 cases(14 eyes). The histological differences between the two groups were compared by hematoxylin and eosin(HE)staining. The expression of VEGF and CCR3 in pterygium and normal Conjunctival tissues were analyzed by immunofluorescence and immunohistochemical. The mRNA expression of VEGF and CCR3 were assessed by Real-time PCR.RESULTS: Compared with normal Conjunctival tissue, the epithelial layer of pterygium was obvious thickening, the stromal layer was more disordered, and the expression levels of VEGF(0.69±0.0875 vs 0.05±0.0024)and CCR3(0.45±0.0248 vs 0.03±0.0074)were significantly elevated(all P

  • Vascular endothelial growth factor and chemokine receptor-3 in the pathogenesis of pterygium
    Press of International Journal of Ophthalmology (IJO PRESS), 2019
    Co-Authors: Hui Liu, Min Zhang
    Abstract:

    AIM: To investigate the expression of vascular endothelial growth factor(VEGF)and chemokine receptor 3(CCR3)in pterygium.METHODS: The expression of VEGF and CCR3 were detected in 18 patients(18 eyes)with pterygium and compared with the normal Conjunctiva Epithelium in other 14 cases(14 eyes). The histological differences between the two groups were compared by hematoxylin and eosin(HE)staining. The expression of VEGF and CCR3 in pterygium and normal Conjunctival tissues were analyzed by immunofluorescence and immunohistochemical. The mRNA expression of VEGF and CCR3 were assessed by Real-time PCR.RESULTS: Compared with normal Conjunctival tissue, the epithelial layer of pterygium was obvious thickening, the stromal layer was more disordered, and the expression levels of VEGF(0.69±0.0875 vs 0.05±0.0024)and CCR3(0.45±0.0248 vs 0.03±0.0074)were significantly elevated(all Pvs 1.00±0.08), and the expression level of CCR3 mRNA was about 160 times as many as Conjunctival tissue(159.60±34.15 vs 1.00±0.09).CONCLUSION: The expression of VEGF and CCR3 in pterygium was significantly increased, suggesting that they may be involved in the occurrence of pterygium and promote the development of the disease

Wang Mingwu - One of the best experts on this subject based on the ideXlab platform.

  • Expression patterns of Conjunctival mucin 5AC and aquaporin 5 in response to acute dry eye stress
    'Public Library of Science (PLoS)', 2017
    Co-Authors: Bhattacharya Dhruva, Wang Mingwu
    Abstract:

    The relationship between aquaporin (AQP) 5 and mucin (MUC) 5AC in the Conjunctiva was investigated in response to acute dry eye (DE) stress. A mixed-mechanism rabbit DE model, in which the main lacrimal gland, Harderian gland, and nictitating membrane were resected, was further explored in this study. Conjunctival impression cytology specimens were harvested before excision (BE) and up to 3 months after excision (AE) in 8 (16 eyes) male New Zealand White rabbits, and immunoblotting was employed to assess the expression of AQP5 and MUC5AC. It was observed that AQP5 and MUC5AC showed a positive, synchronous expression pattern with progressive upregulation at protein level up to 2 months AE. At 3 months, the expression of both proteins decreased, but was still higher than that of BE. Such a synchronous relationship was further observed in mouse Conjunctiva Epithelium primary cells under hyperosmotic condition. Moreover, the co-immunoprecipitation of AQP5 and MUC5AC suggested a possible physical interaction between the two molecules. Our data indicates that Conjunctival AQP5 and MUC5AC act synchronously in response to acute DE stress.Arizona Biomedical Research Commission Research [ADHS14-082988]; Department of Ophthalmology and Vision Science at University of ArizonaOpen access journal.This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu