The Experts below are selected from a list of 297 Experts worldwide ranked by ideXlab platform
Claire Quentel - One of the best experts on this subject based on the ideXlab platform.
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EROD activity and antioxidant defenses of sea bass (Dicentrarchus labrax) after an in vivo chronic hydrocarbon pollution followed by a post-exposure period
Environmental science and pollution research international, 2014Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Claire QuentelAbstract:Chronic concentrations of polycyclic aromatic hydrocarbons (PAHs) have been commonly detected in international estuaries ecosystems. Reliable indicators still need to be found in order to properly assess the impact of PAHs in Fish. After an in vivo chronic exposure to hydrocarbons, the enzymatic activity of 7-ethoxyresorufin O-deethylase (EROD) and the antioxidant defense system were assessed in sea bass, Dicentrarchus labrax. A total of 45 Fish were exposed to the water-soluble fraction of Arabian crude oil, similar to a complex pollution by hydrocarbons chronically observed in situ, while 45 other control Fish sustained the same experimental conditions in clean seawater. Fish samples were made after a 21-day exposure period and after a 15-day recovery period in clean fresh water. Throughout the experiment, liver EROD activity was significantly higher in Contaminated Fish than in control Fish. In addition, nonenzymatic (total glutathione) and enzymatic (GPx, SOD, and CAT) antioxidant defense parameters measured in liver were not significantly different in Fish. Furthermore, in gills, glutathione content had significantly increased while SOD activity had significantly decreased in Contaminated Fish compared to controls. On the other hand, CAT and GPx activities were not affected. Chronic exposure to PAHs disturbing the first step (SOD) and inhibiting the second step (GPx and CAT) could induce oxidative stress in tissues by the formation of oxygen radicals. After the postexposure period, there was no significant difference between control and Contaminated Fish in any of the antioxidant defense parameters measured in gills, attesting to the reversibility of the effects.
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effects of in vivo chronic exposure to pendimethalin prowl 400 on sanitary status and the immune system in rainbow trout oncorhynchus mykiss
Science of The Total Environment, 2012Co-Authors: Morgane Danion, François Lamour, Stephane Le Floch, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Effects of in vivo chronic exposure to pendimethalin/Prowl 400® on sanitary status and the immune system in rainbow trout (Oncorhynchus mykiss)
The Science of the total environment, 2012Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Bioconcentration and immunotoxicity of an experimental oil spill in European sea bass (Dicentrarchus labrax L.).
Ecotoxicology and Environmental Safety, 2011Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Julien Guyomarch, Claire QuentelAbstract:The effects of Polycyclic Aromatic Hydrocarbons (PAHs) resulting from a water soluble fraction (WSF) of an Arabian crude oil were tested in vivo on the bioconcentration in muscles and on immune parameters in sea bass, Dicentrarchus labrax. After 15 days of acclimation, Fish were acutely exposed (48 h) to the WSF of 25 g of oil, and then returned to clean sea water for a 15 day recovery period. PAH concentration in the WSF at the beginning of the exposure was estimated to 773±187 ng L⁻¹ similar to that observed in the marine environment after an oil spill. The WSF in the experimental system was composed by lightest PAH compounds and did not remain constant during the two days of exposure. Just after exposure to the WSF, a total mean concentration of 148±46 μg kg⁻¹ of PAHs was found in Contaminated Fish muscle, composed of parent and alkylated naphthalene compounds (86.5%), benzo[a]pyrene (10.1%) and benzo[b+k]fluoranthene (3.4%). In addition, a decrease of leucocytes counts due to a lymphopenia and granulopenia and an increase of the haemolytic activity of the alternative pathway (ACH₅₀) were noted. After a 15 day recovery period, haematocrit was decreased whereas effects on the blood granulocytes of Fish seemed to be reversible, contrary to the specific immune system and quality of flesh. In fact, Contaminated Fish had still less lymphocyte cells compared to controls Fish and their flesh were still Contaminated by naphthalene and benzo[a]pyrene creating a risk for human consumers.
Morgane Danion - One of the best experts on this subject based on the ideXlab platform.
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EROD activity and antioxidant defenses of sea bass (Dicentrarchus labrax) after an in vivo chronic hydrocarbon pollution followed by a post-exposure period
Environmental science and pollution research international, 2014Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Claire QuentelAbstract:Chronic concentrations of polycyclic aromatic hydrocarbons (PAHs) have been commonly detected in international estuaries ecosystems. Reliable indicators still need to be found in order to properly assess the impact of PAHs in Fish. After an in vivo chronic exposure to hydrocarbons, the enzymatic activity of 7-ethoxyresorufin O-deethylase (EROD) and the antioxidant defense system were assessed in sea bass, Dicentrarchus labrax. A total of 45 Fish were exposed to the water-soluble fraction of Arabian crude oil, similar to a complex pollution by hydrocarbons chronically observed in situ, while 45 other control Fish sustained the same experimental conditions in clean seawater. Fish samples were made after a 21-day exposure period and after a 15-day recovery period in clean fresh water. Throughout the experiment, liver EROD activity was significantly higher in Contaminated Fish than in control Fish. In addition, nonenzymatic (total glutathione) and enzymatic (GPx, SOD, and CAT) antioxidant defense parameters measured in liver were not significantly different in Fish. Furthermore, in gills, glutathione content had significantly increased while SOD activity had significantly decreased in Contaminated Fish compared to controls. On the other hand, CAT and GPx activities were not affected. Chronic exposure to PAHs disturbing the first step (SOD) and inhibiting the second step (GPx and CAT) could induce oxidative stress in tissues by the formation of oxygen radicals. After the postexposure period, there was no significant difference between control and Contaminated Fish in any of the antioxidant defense parameters measured in gills, attesting to the reversibility of the effects.
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effects of in vivo chronic exposure to pendimethalin prowl 400 on sanitary status and the immune system in rainbow trout oncorhynchus mykiss
Science of The Total Environment, 2012Co-Authors: Morgane Danion, François Lamour, Stephane Le Floch, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Effects of in vivo chronic exposure to pendimethalin/Prowl 400® on sanitary status and the immune system in rainbow trout (Oncorhynchus mykiss)
The Science of the total environment, 2012Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Bioconcentration and immunotoxicity of an experimental oil spill in European sea bass (Dicentrarchus labrax L.).
Ecotoxicology and Environmental Safety, 2011Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Julien Guyomarch, Claire QuentelAbstract:The effects of Polycyclic Aromatic Hydrocarbons (PAHs) resulting from a water soluble fraction (WSF) of an Arabian crude oil were tested in vivo on the bioconcentration in muscles and on immune parameters in sea bass, Dicentrarchus labrax. After 15 days of acclimation, Fish were acutely exposed (48 h) to the WSF of 25 g of oil, and then returned to clean sea water for a 15 day recovery period. PAH concentration in the WSF at the beginning of the exposure was estimated to 773±187 ng L⁻¹ similar to that observed in the marine environment after an oil spill. The WSF in the experimental system was composed by lightest PAH compounds and did not remain constant during the two days of exposure. Just after exposure to the WSF, a total mean concentration of 148±46 μg kg⁻¹ of PAHs was found in Contaminated Fish muscle, composed of parent and alkylated naphthalene compounds (86.5%), benzo[a]pyrene (10.1%) and benzo[b+k]fluoranthene (3.4%). In addition, a decrease of leucocytes counts due to a lymphopenia and granulopenia and an increase of the haemolytic activity of the alternative pathway (ACH₅₀) were noted. After a 15 day recovery period, haematocrit was decreased whereas effects on the blood granulocytes of Fish seemed to be reversible, contrary to the specific immune system and quality of flesh. In fact, Contaminated Fish had still less lymphocyte cells compared to controls Fish and their flesh were still Contaminated by naphthalene and benzo[a]pyrene creating a risk for human consumers.
François Lamour - One of the best experts on this subject based on the ideXlab platform.
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EROD activity and antioxidant defenses of sea bass (Dicentrarchus labrax) after an in vivo chronic hydrocarbon pollution followed by a post-exposure period
Environmental science and pollution research international, 2014Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Claire QuentelAbstract:Chronic concentrations of polycyclic aromatic hydrocarbons (PAHs) have been commonly detected in international estuaries ecosystems. Reliable indicators still need to be found in order to properly assess the impact of PAHs in Fish. After an in vivo chronic exposure to hydrocarbons, the enzymatic activity of 7-ethoxyresorufin O-deethylase (EROD) and the antioxidant defense system were assessed in sea bass, Dicentrarchus labrax. A total of 45 Fish were exposed to the water-soluble fraction of Arabian crude oil, similar to a complex pollution by hydrocarbons chronically observed in situ, while 45 other control Fish sustained the same experimental conditions in clean seawater. Fish samples were made after a 21-day exposure period and after a 15-day recovery period in clean fresh water. Throughout the experiment, liver EROD activity was significantly higher in Contaminated Fish than in control Fish. In addition, nonenzymatic (total glutathione) and enzymatic (GPx, SOD, and CAT) antioxidant defense parameters measured in liver were not significantly different in Fish. Furthermore, in gills, glutathione content had significantly increased while SOD activity had significantly decreased in Contaminated Fish compared to controls. On the other hand, CAT and GPx activities were not affected. Chronic exposure to PAHs disturbing the first step (SOD) and inhibiting the second step (GPx and CAT) could induce oxidative stress in tissues by the formation of oxygen radicals. After the postexposure period, there was no significant difference between control and Contaminated Fish in any of the antioxidant defense parameters measured in gills, attesting to the reversibility of the effects.
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effects of in vivo chronic exposure to pendimethalin prowl 400 on sanitary status and the immune system in rainbow trout oncorhynchus mykiss
Science of The Total Environment, 2012Co-Authors: Morgane Danion, François Lamour, Stephane Le Floch, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Effects of in vivo chronic exposure to pendimethalin/Prowl 400® on sanitary status and the immune system in rainbow trout (Oncorhynchus mykiss)
The Science of the total environment, 2012Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Bioconcentration and immunotoxicity of an experimental oil spill in European sea bass (Dicentrarchus labrax L.).
Ecotoxicology and Environmental Safety, 2011Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Julien Guyomarch, Claire QuentelAbstract:The effects of Polycyclic Aromatic Hydrocarbons (PAHs) resulting from a water soluble fraction (WSF) of an Arabian crude oil were tested in vivo on the bioconcentration in muscles and on immune parameters in sea bass, Dicentrarchus labrax. After 15 days of acclimation, Fish were acutely exposed (48 h) to the WSF of 25 g of oil, and then returned to clean sea water for a 15 day recovery period. PAH concentration in the WSF at the beginning of the exposure was estimated to 773±187 ng L⁻¹ similar to that observed in the marine environment after an oil spill. The WSF in the experimental system was composed by lightest PAH compounds and did not remain constant during the two days of exposure. Just after exposure to the WSF, a total mean concentration of 148±46 μg kg⁻¹ of PAHs was found in Contaminated Fish muscle, composed of parent and alkylated naphthalene compounds (86.5%), benzo[a]pyrene (10.1%) and benzo[b+k]fluoranthene (3.4%). In addition, a decrease of leucocytes counts due to a lymphopenia and granulopenia and an increase of the haemolytic activity of the alternative pathway (ACH₅₀) were noted. After a 15 day recovery period, haematocrit was decreased whereas effects on the blood granulocytes of Fish seemed to be reversible, contrary to the specific immune system and quality of flesh. In fact, Contaminated Fish had still less lymphocyte cells compared to controls Fish and their flesh were still Contaminated by naphthalene and benzo[a]pyrene creating a risk for human consumers.
Stéphane Le Floch - One of the best experts on this subject based on the ideXlab platform.
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EROD activity and antioxidant defenses of sea bass (Dicentrarchus labrax) after an in vivo chronic hydrocarbon pollution followed by a post-exposure period
Environmental science and pollution research international, 2014Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Claire QuentelAbstract:Chronic concentrations of polycyclic aromatic hydrocarbons (PAHs) have been commonly detected in international estuaries ecosystems. Reliable indicators still need to be found in order to properly assess the impact of PAHs in Fish. After an in vivo chronic exposure to hydrocarbons, the enzymatic activity of 7-ethoxyresorufin O-deethylase (EROD) and the antioxidant defense system were assessed in sea bass, Dicentrarchus labrax. A total of 45 Fish were exposed to the water-soluble fraction of Arabian crude oil, similar to a complex pollution by hydrocarbons chronically observed in situ, while 45 other control Fish sustained the same experimental conditions in clean seawater. Fish samples were made after a 21-day exposure period and after a 15-day recovery period in clean fresh water. Throughout the experiment, liver EROD activity was significantly higher in Contaminated Fish than in control Fish. In addition, nonenzymatic (total glutathione) and enzymatic (GPx, SOD, and CAT) antioxidant defense parameters measured in liver were not significantly different in Fish. Furthermore, in gills, glutathione content had significantly increased while SOD activity had significantly decreased in Contaminated Fish compared to controls. On the other hand, CAT and GPx activities were not affected. Chronic exposure to PAHs disturbing the first step (SOD) and inhibiting the second step (GPx and CAT) could induce oxidative stress in tissues by the formation of oxygen radicals. After the postexposure period, there was no significant difference between control and Contaminated Fish in any of the antioxidant defense parameters measured in gills, attesting to the reversibility of the effects.
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Effects of in vivo chronic exposure to pendimethalin/Prowl 400® on sanitary status and the immune system in rainbow trout (Oncorhynchus mykiss)
The Science of the total environment, 2012Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Rami Kanan, Claire QuentelAbstract:Abstract The in vivo effects of the herbicide active substance (AS) pendimethalin (alone and with Prowl 400® adjuvant) were evaluated on sanitary status i.e. the health status with regard to chemical pollution and on the physiological state via the immune system in rainbow trout, Oncorhynchus mykiss . Four nominal exposure conditions were tested: i) control (C), ii) AS at 500 ng L − 1 (P500), iii) AS at 800 ng L − 1 (P800) and iv) Prowl 400® at 500 ng L − 1 (Pw). After a 28 day exposure period (D28), 10 Fish were sampled for each condition and 10 other after a 15 day recovery period in clean fresh water (D43). Pendimethalin concentrations in the exposure water and muscles were followed. White blood cell counts, differential leucocyte counts, cell mortality and phagocytosis activity were measured. Haemolytic alternative complement activity, lysozyme concentration and stress parameters were analyzed. The resulting concentration of pendimethalin in the exposure water was lower than the expected concentration. At D28, the concentration quantified in the Contaminated Fish was negligible in comparison with the Reference Dose for Oral Exposure estimated by US-EPA's Integrated Risk Information System. Leucopenia was noted in all Contaminated Fish. A decrease in phagocytosis activity and ACH 50 was also observed in Contaminated Fish by P800 and Pw. Disturbed lysozyme activity was noted only in Fish exposed to Pw. Furthermore, during exposure to a similar concentration of pendimethalin, the commercial product seemed to be more immunotoxic than the AS alone. Finally, at D43, the effects proved reversible for sanitary status while immunity was still disturbed in Contaminated Fish by P800 and Pw.
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Bioconcentration and immunotoxicity of an experimental oil spill in European sea bass (Dicentrarchus labrax L.).
Ecotoxicology and Environmental Safety, 2011Co-Authors: Morgane Danion, Stéphane Le Floch, François Lamour, Julien Guyomarch, Claire QuentelAbstract:The effects of Polycyclic Aromatic Hydrocarbons (PAHs) resulting from a water soluble fraction (WSF) of an Arabian crude oil were tested in vivo on the bioconcentration in muscles and on immune parameters in sea bass, Dicentrarchus labrax. After 15 days of acclimation, Fish were acutely exposed (48 h) to the WSF of 25 g of oil, and then returned to clean sea water for a 15 day recovery period. PAH concentration in the WSF at the beginning of the exposure was estimated to 773±187 ng L⁻¹ similar to that observed in the marine environment after an oil spill. The WSF in the experimental system was composed by lightest PAH compounds and did not remain constant during the two days of exposure. Just after exposure to the WSF, a total mean concentration of 148±46 μg kg⁻¹ of PAHs was found in Contaminated Fish muscle, composed of parent and alkylated naphthalene compounds (86.5%), benzo[a]pyrene (10.1%) and benzo[b+k]fluoranthene (3.4%). In addition, a decrease of leucocytes counts due to a lymphopenia and granulopenia and an increase of the haemolytic activity of the alternative pathway (ACH₅₀) were noted. After a 15 day recovery period, haematocrit was decreased whereas effects on the blood granulocytes of Fish seemed to be reversible, contrary to the specific immune system and quality of flesh. In fact, Contaminated Fish had still less lymphocyte cells compared to controls Fish and their flesh were still Contaminated by naphthalene and benzo[a]pyrene creating a risk for human consumers.
Christel Lefrancois - One of the best experts on this subject based on the ideXlab platform.
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PCB contamination does not alter aerobic metabolism and tolerance to hypoxia of juvenile sole (Solea solea L. 1758)
Aquatic Toxicology, 2013Co-Authors: Marcella Cannas, Fabrizio Atzori, François Rupsard, Paco Bustamante, Véronique Loizeau, Christel LefrancoisAbstract:Coastal habitats play a major role as nurseries for many Fish species; however, they are also submitted to pollutants and oxygen fluctuations. Fry's concept of metabolic scope for activity was used to evaluate the effect of polychlorinated biphenyls (PCBs) on the aerobic metabolism in juvenile common sole (0-1 year old). Aerobic metabolic scope (AMS) in control and PCB-Contaminated Fish via food pathway was determined using respirometry techniques. Furthermore, the hypoxia tolerance in control and PCB-Contaminated Fish was evaluated by assessing their critical oxygen concentration (O2crit). Our results showed that while PCB-Contaminated Fish were able to maintain a constant AMS, PCBs tend to affect their aerobic metabolism by acting on standard metabolic rate, maximal oxygen consumption (MO2max) in hypoxia and O2crit. In conclusion, we can hypothesise that a long-term exposure to PCBs may decrease their tolerance to hypoxia and consequently impair the survival and/or development of soles in their natural environment.
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PCBs contamination does not alter aerobic metabolism and tolerance to hypoxia of juvenile sole (Solea solea L. 1758)
Aquatic toxicology (Amsterdam Netherlands), 2012Co-Authors: Marcella Cannas, Fabrizio Atzori, François Rupsard, Paco Bustamante, Véronique Loizeau, Christel LefrancoisAbstract:Coastal habitats play a major role as nurseries for many Fish species; however, they are also submitted to pollutants and oxygen fluctuations. Fry's concept of metabolic scope for activity was used to evaluate the effect of polychlorinated biphenyls (PCBs) on the aerobic metabolism in juvenile common sole (0–1 year old). Aerobic metabolic scope (AMS) in control and PCB-Contaminated Fish via food pathway was determined using respirometry techniques. Furthermore, the hypoxia tolerance in control and PCB-Contaminated Fish was evaluated by assessing their critical oxygen concentration (O2crit). Our results showed that while PCB-Contaminated Fish were able to maintain a constant AMS and O2crit, PCBs tend to affect their aerobic metabolism by acting on maximal oxygen consumption (MO2max) in hypoxia and standard metabolic rate, but only at the highest PCB concentration between 30 and 60 days of exposure. In conclusion, we can hypothetise that the tested PCB-exposures may not impair the tolerance to hypoxia and the survival of common sole in their natural environment.