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Zhongyi Zhang - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of 40 isolated rehmannia glutinosa myb family genes and their expression profiles in response to shading and Continuous Cropping
International Journal of Molecular Sciences, 2015Co-Authors: Fengqing Wang, Xinjian Chen, Mingjie Li, Lina Wang, Zhongyi ZhangAbstract:The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under Continuous Cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa.
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Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping
MDPI AG, 2015Co-Authors: Fengqing Wang, Xinjian Chen, Lina Wang, Yanfei Suo, He Wei, Caixia Xie, Zhongyi ZhangAbstract:The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under Continuous Cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa
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differential mirna expression in rehmannia glutinosa plants subjected to Continuous Cropping
BMC Plant Biology, 2011Co-Authors: Yanhui Yang, Xinjian Chen, Haixia Xu, Junying Chen, Juan Li, Zhongyi ZhangAbstract:Background The productivity of the medicinally significant perennial herb Rehmannia glutinosa is severely affected after the first year of Cropping. While there is some information available describing the physiological and environmental causes of this yield decline, there is as yet no data regarding the changes in gene expression which occur when the species is Continuously cropped.
Zhihui Cheng - One of the best experts on this subject based on the ideXlab platform.
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garlic allelochemical diallyl disulfide alleviates autotoxicity in the root exudates caused by long term Continuous Cropping of tomato
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Muhammad Ali, Fang Cheng, Ce Liu, Rui Deng, Zhihui ChengAbstract:Continuous Cropping obstacles seriously affect the sustainable production of tomatoes (Solanum lycopersicum L.). Researchers have found that interCropping with garlic (Allium sativum L.) could alleviate tomato Continuous Cropping obstacles. Diallyl disulfide (DADS) is the main allelochemical in garlic. However, the mechanism of DADS in alleviating tomato Continuous Cropping obstacles is still unknown. In this research, aqueous extracts of tomato Continuous Cropping soil were used to simulate the Continuous Cropping condition of tomato. Our results showed that DADS increased root activity and chlorophyll content and improved the activity of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and phenylalanine ammonia-lyase (PAL)) and the metabolism of nonenzymatic antioxidants (glutathione (GSH) and oxidized glutathione (GSSG)) in tomato plants. DADS treatment reduced the content of fatty acid esters in tomato root exudates (e.g., palmitate methyl ester, palmitoleic acid methyl ester, oleic acid methyl ester) and increased the level of substances such as dibutyl phthalate and 2,2'-methylenebis(6-tert-butyl-4-methylphenol). The higher concentrations of palmitate methyl ester inhibited tomato hypocotyl growth, while oleic acid methyl ester inhibited tomato root growth. Moreover, the application of DADS significantly inhibited the secretion of these esters in the root exudates. Therefore, it suggests that DADS may increase tomato resistance and promote tomato plant growth by increasing root activity and photosynthetic capacity and development to reduce autotoxicity of tomato.
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a green garlic allium sativum l based interCropping system reduces the strain of Continuous monoCropping in cucumber cucumis sativus l by adjusting the micro ecological environment of soil
PeerJ, 2019Co-Authors: Zhihui Cheng, Xuemei Xiao, Jianming XieAbstract:The Continuous Cropping obstacle of cucumber (Cucumis sativus L.) under facility cultivation is more prevalent in China. This is associated with an imbalance in soil microbial and ecological environment in long-term monocultures. It was postulated that interCropping with green garlic would relieve the Continuous Cropping obstacle of cucumber by altering the soil micro-ecology status. A pot-based experiment was conducted to investigate the green garlic-cucumber interCropping and cucumber monoCropping systems. The results showed that the cucumber shoot biomass was improved by interCropping with green garlic. However, the population of soil bacteria and actinomycetes increased, while the fungal population decreased. The fatty acid methyl ester (FAME) profiles indicated that soil contained more fungal FAME biomarkers (18:1ω9c, 18:2ω6, 9) and higher fungal:bacterial ratio in the monoculture system, whereas clustering of more bacterial FAME biomarkers (cy17:0, cy19:0, 16:1ω7c10, Me16:0, 10Me17:0, 10Me18:0) was observed under interCropping conditions. Moreover, significantly (P < 0.05) higher soil invertase and alkaline phosphatase activities, organic matter, and available N, P and K contents were observed under interCropping systems. These were high in both bulk and rhizosphere soils in the interCropping system when compared to monoCropping system. These findings suggest that interCropping with green garlic can alleviate Continuous Cropping obstacle of cucumber by improving the diverse composition of soil microbial community, enzyme activities, and nutrient availability.
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transcriptomic insights into the allelopathic effects of the garlic allelochemical diallyl disulfide on tomato roots
Scientific Reports, 2016Co-Authors: Fang Cheng, Zhihui Cheng, Huanwen MengAbstract:Garlic is an allelopathic crop that can alleviate the obstacles to Continuous Cropping of vegetable crops. Diallyl disulfide (DADS), one of the most important allelochemicals in garlic, promotes tomato root growth. Therefore, the global transcriptome profiles of DADS-treated tomato roots over time were investigated to reveal the potential growth-promoting mechanisms. We detected 1828, 1296 and 1190 differentially expressed genes (DEGs) in the 4, 24 and 48 h samples, respectively. Most DEGs involved in assimilatory sulfate reduction and glutathione metabolism were up-regulated after short-term (4 h) DADS treatment. In addition, increased activity of defensive enzymes and up-regulation of six peroxidase genes were observed, suggesting that DADS could induce tomato resistance. In plant-pathogen interactions, DEGs related to calcium signaling were primarily inhibited, while those encoding pathogenesis-related proteins were primarily up-regulated. Although plant hormone synthesis and signal transduction were both significantly affected by DADS, the expression trends of the genes in these two pathways were conflicting. This research provides comprehensive information concerning the changes in the tomato root transcriptome affected by DADS and may help direct further studies on DADS-responsive genes to enhance the current understanding of the mechanisms by which DADS alleviates the obstacles to Continuous Cropping.
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soil chemical property changes in eggplant garlic relay interCropping systems under Continuous Cropping
PLOS ONE, 2014Co-Authors: Mengyi Wang, Cuinan Wu, Zhihui Cheng, Huanwen Meng, Mengru Zhang, Hongjing ZhangAbstract:Soil sickness is a critical problem for eggplant (Solanum melongena L.) under Continuous Cropping that affects sustainable eggplant production. Relay interCropping is a significant technique on promoting soil quality, improving eco-environment, and raising output. Field experiments were conducted from September 2010 to November 2012 in northwest China to determine the effects of relay interCropping eggplant with garlic (Allium sativum L.) on soil enzyme activities, available nutrient contents, and pH value under a plastic tunnel. Three treatments were in triplicate using randomized block design: eggplant monoculture (CK), eggplant relay interCropping with normal garlic (NG) and eggplant relay interCropping with green garlic (GG). The major results are as follows: (1) the activities of soil invertase, urease, and alkaline phosphatase were generally enhanced in NG and GG treatments; (2) relay interCropping significantly increased the soil available nutrient contents, and they were mostly higher in GG than NG. On April 11, 2011, the eggplant/garlic co-growth stage, the available nitrogen content in GG was 76.30 mg·kg−1, significantly higher than 61.95 mg·kg−1 in NG. For available potassium on April 17, 2012, they were 398.48 and 387.97 mg·kg−1 in NG and GG, both were significantly higher than 314.84 mg·kg−1 in CK; (3) the soil pH showed a significantly higher level in NG treatment, but lower in GG treatment compared with CK. For the last samples in 2012, soil pH in NG and GG were 7.70 and 7.46, the highest and lowest one among them; (4) the alkaline phosphatase activity and pH displayed a similar decreasing trend with Continuous Cropping. These findings indicate that relay interCropping eggplant with garlic could be an ideal farming system to effectively improve soil nutrient content, increase soil fertility, and alleviate soil sickness to some extent. These findings are important in helping to develop sustainable eggplant production.
Xinjian Chen - One of the best experts on this subject based on the ideXlab platform.
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identification and characterization of 40 isolated rehmannia glutinosa myb family genes and their expression profiles in response to shading and Continuous Cropping
International Journal of Molecular Sciences, 2015Co-Authors: Fengqing Wang, Xinjian Chen, Mingjie Li, Lina Wang, Zhongyi ZhangAbstract:The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under Continuous Cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa.
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Identification and Characterization of 40 Isolated Rehmannia glutinosa MYB Family Genes and Their Expression Profiles in Response to Shading and Continuous Cropping
MDPI AG, 2015Co-Authors: Fengqing Wang, Xinjian Chen, Lina Wang, Yanfei Suo, He Wei, Caixia Xie, Zhongyi ZhangAbstract:The v-myb avian myeloblastosis viral oncogene homolog (MYB) superfamily constitutes one of the most abundant groups of transcription factors (TFs) described in plants. To date, little is known about the MYB genes in Rehmannia glutinosa. Forty unique MYB genes with full-length cDNA sequences were isolated. These 40 genes were grouped into five categories, one R1R2R3-MYB, four TRFL MYBs, four SMH MYBs, 25 R2R3-MYBs, and six MYB-related members. The MYB DNA-binding domain (DBD) sequence composition was conserved among proteins of the same subgroup. As expected, most of the closely related members in the phylogenetic tree exhibited common motifs. Additionally, the gene structure and motifs of the R. glutinosa MYB genes were analyzed. MYB gene expression was analyzed in the leaf and the tuberous root under two abiotic stress conditions. Expression profiles showed that most R. glutinosa MYB genes were expressed in the leaf and the tuberous root, suggesting that MYB genes are involved in various physiological and developmental processes in R. glutinosa. Seven MYB genes were up-regulated in response to shading in at least one tissue. Two MYB genes showed increased expression and 13 MYB genes showed decreased expression in the tuberous root under Continuous Cropping. This investigation is the first comprehensive study of the MYB gene family in R. glutinosa
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differential mirna expression in rehmannia glutinosa plants subjected to Continuous Cropping
BMC Plant Biology, 2011Co-Authors: Yanhui Yang, Xinjian Chen, Haixia Xu, Junying Chen, Juan Li, Zhongyi ZhangAbstract:Background The productivity of the medicinally significant perennial herb Rehmannia glutinosa is severely affected after the first year of Cropping. While there is some information available describing the physiological and environmental causes of this yield decline, there is as yet no data regarding the changes in gene expression which occur when the species is Continuously cropped.
Qirong Shen - One of the best experts on this subject based on the ideXlab platform.
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the effect of long term Continuous Cropping of black pepper on soil bacterial communities as determined by 454 pyrosequencing
PLOS ONE, 2015Co-Authors: Wu Xiong, Ruifu Zhang, Zhigang Li, Huasong Wu, Rong Li, Qirong ShenAbstract:In the present study, 3 replanted black pepper orchards with Continuously Cropping histories for 10, 21, and 55 years in tropical China, were selected for investigating the effect of monoculture on soil physiochemical properties, enzyme activities, bacterial abundance, and bacterial community structures. Results showed long-term Continuous Cropping led to a significant decline in soil pH, organic matter contents, enzymatic activities, and resulted in a decrease in soil bacterial abundance. 454 pyrosequencing analysis of 16S rRNA genes revealed that the Acidobacteria and Proteobacteria were the main phyla in the replanted black pepper orchard soils, comprising up to 73.82% of the total sequences; the relative abundances of Bacteroidetes and Firmicutes phyla decreased with long-term Continuous Cropping; and at genus level, the Pseudomonas abundance significantly depleted after 21 years Continuous Cropping. In addition, bacterial diversity significantly decreased after 55 years black pepper Continuous Cropping; obvious variations for community structures across the 3 time-scale replanted black pepper orchards were observed, suggesting monoculture duration was the major determinant for bacterial community structure. Overall, Continuous Cropping during black pepper cultivation led to a significant decline in soil pH, organic matter contents, enzymatic activities, resulted a decrease in soil bacterial abundance, and altered soil microbial community membership and structure, which in turn resulted in black pepper poor growth in the Continuous Cropping system.
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Different Continuous Cropping Spans Significantly Affect Microbial Community Membership and Structure in a Vanilla-Grown Soil as Revealed by Deep Pyrosequencing
Microbial ecology, 2014Co-Authors: Wu Xiong, Qingyun Zhao, Jun Zhao, Weibing Xun, Ruifu Zhang, Qirong ShenAbstract:In the present study, soil bacterial and fungal communities across vanilla Continuous Cropping time-series fields were assessed through deep pyrosequencing of 16S ribosomal RNA (rRNA) genes and internal transcribed spacer (ITS) regions. The results demonstrated that the long-term monoculture of vanilla significantly altered soil microbial communities. Soil fungal diversity index increased with consecutive Cropping years, whereas soil bacterial diversity was relatively stable. Bray-Curtis dissimilarity cluster and UniFrac-weighted principal coordinate analysis (PCoA) revealed that monoculture time was the major determinant for fungal community structure, but not for bacterial community structure. The relative abundances (RAs) of the Firmicutes, Actinobacteria, Bacteroidetes, and Basidiomycota phyla were depleted along the years of vanilla monoculture. Pearson correlations at the phyla level demonstrated that Actinobacteria, Armatimonadetes, Bacteroidetes, Verrucomicrobia, and Firmicutes had significant negative correlations with vanilla disease index (DI), while no significant correlation for fungal phyla was observed. In addition, the amount of the pathogen Fusarium oxysporum accumulated with increasing years and was significantly positively correlated with vanilla DI. By contrast, the abundance of beneficial bacteria, including Bradyrhizobium and Bacillus, significantly decreased over time. In sum, soil weakness and vanilla stem wilt disease after long-term Continuous Cropping can be attributed to the alteration of the soil microbial community membership and structure, i.e., the reduction of the beneficial microbes and the accumulation of the fungal pathogen.
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variation of rhizosphere bacterial community in watermelon Continuous mono Cropping soil by long term application of a novel bioorganic fertilizer
Microbiological Research, 2014Co-Authors: Ning Ling, Jun Zhao, Waseem Raza, Kaiying Deng, Yang Song, Yunchen Wu, Qiwei Huang, Qirong ShenAbstract:The application method for a novel bioorganic fertilizer (BIO) was developed to improve its biocontrol efficacy of Fusarium wilt (Ling et al. 2010). However, its efficacy on controlling Fusarium wilt and the variations of microbial community after long-term application for watermelon production had not been elucidated. To clarify, a 4-years pot experiment of mono-Cropping watermelon was conducted. The results revealed that though the disease incidences were increased in all treatments with the increase of Continuous Cropping years, the treatment of BIO application both in nursery and pot soil always maintained the lowest disease incidence. The real-time PCR results showed that the population of Paenibacillus polymyxa was decreased with Continuous Cropping years, but in all seasons, the treatment with BIO application both in nursery and pot soil had a highest population of P. polymyxa than the other treatments. On the other hand, the abundance of the pathogen FON was increased with the increase of Continuous Cropping years and the lowest rate of increase was found by BIO application in both nursery and pot soil. DGGE patterns showed that the bacterial diversity was weakened after mono-Cropping of watermelon for 4 years, but the consecutive applications of BIO at nursery and transplanting stage resulted in the minimal change of bacterial diversity. More detailed differences on bacterial diversity between control and double application of BIO treatment after 4-years monoculture were analyzed by 454 pyrosequencing, which showed the dominant phyla found in both samples were Firmicutes, Proteobacteria and Actinobacteria, and the consecutive applications of BIO recruited more beneficial bacteria than control, such as Bacillus, Paenibacillus, Haliangium, Streptomyces. Overall, these results, to a certain extent, approved that the consecutive applications of BIO at nursery and transplanting stage could effectively suppress watermelon Fusarium wilt by regulating the rhizosphere bacterial diversity. These results could give some clues that how to regulate the soil microbial community to an appropriate level which can keep the plant healthy and thus control the soil-borne diseases.
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trichoderma harzianum sqr t037 rapidly degrades allelochemicals in rhizospheres of Continuously cropped cucumbers
Applied Microbiology and Biotechnology, 2011Co-Authors: Lihua Chen, Xingming Yang, Waseem Raza, Junhua Li, Fengge Zhang, Qirong ShenAbstract:To alleviate the stress of Continuous Cropping for cucumber Continuous Cropping (CCC) system, a beneficial fungus Trichoderma harzianum SQR-T037 (SQR-T037) was isolated and applied to soil to degrade allelochemicals exuded from cucumber plants in a Rhizobox experiment. The following phenolic acids (PAs), classified as allelochemicals, were isolated and identified from cucumber rhizospheres: 4-hydroxybenzoic acid, vanillic acid, ferulic acid, benzoic acid, 3-phenylpropionic acid, and cinnamic acid. Mixed PAs added in potato dextrose broth, each with 0.2 gram per liter, were completely degraded by SQR-T037 after 170 h of incubation. In Rhizobox experiments, inoculation of SQR-T037 in the CCC soil also degraded the PAs exuded from cucumber plant roots. This degradation was 88.8% for 4-hydroxybenzoic acid, 90% for vanillic acid, 95% for benzoic acid, and 100% for ferulic acid, 3-phenylpropionic acid, and cinnamic acid at 45 days after plantation. Simultaneously, a significant (p ≥ 0.05) decrease in the disease index of Fusarium wilt and an increase in dry weights of cucumber plants were obtained in pot experiments by application of SQR-T037. This was mostly attributed to degradation of PAs exuded from cucumber roots in CCC soil by SQR-T037 and alleviation of the allelopathic stress. Application of beneficial microorganisms, such as SQR-T037 that biodegrades allelochemicals, is a highly efficient way to resolve the problems associated with Continuous Cropping system.
Fang Cheng - One of the best experts on this subject based on the ideXlab platform.
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garlic allelochemical diallyl disulfide alleviates autotoxicity in the root exudates caused by long term Continuous Cropping of tomato
Journal of Agricultural and Food Chemistry, 2020Co-Authors: Muhammad Ali, Fang Cheng, Ce Liu, Rui Deng, Zhihui ChengAbstract:Continuous Cropping obstacles seriously affect the sustainable production of tomatoes (Solanum lycopersicum L.). Researchers have found that interCropping with garlic (Allium sativum L.) could alleviate tomato Continuous Cropping obstacles. Diallyl disulfide (DADS) is the main allelochemical in garlic. However, the mechanism of DADS in alleviating tomato Continuous Cropping obstacles is still unknown. In this research, aqueous extracts of tomato Continuous Cropping soil were used to simulate the Continuous Cropping condition of tomato. Our results showed that DADS increased root activity and chlorophyll content and improved the activity of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and phenylalanine ammonia-lyase (PAL)) and the metabolism of nonenzymatic antioxidants (glutathione (GSH) and oxidized glutathione (GSSG)) in tomato plants. DADS treatment reduced the content of fatty acid esters in tomato root exudates (e.g., palmitate methyl ester, palmitoleic acid methyl ester, oleic acid methyl ester) and increased the level of substances such as dibutyl phthalate and 2,2'-methylenebis(6-tert-butyl-4-methylphenol). The higher concentrations of palmitate methyl ester inhibited tomato hypocotyl growth, while oleic acid methyl ester inhibited tomato root growth. Moreover, the application of DADS significantly inhibited the secretion of these esters in the root exudates. Therefore, it suggests that DADS may increase tomato resistance and promote tomato plant growth by increasing root activity and photosynthetic capacity and development to reduce autotoxicity of tomato.
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transcriptomic insights into the allelopathic effects of the garlic allelochemical diallyl disulfide on tomato roots
Scientific Reports, 2016Co-Authors: Fang Cheng, Zhihui Cheng, Huanwen MengAbstract:Garlic is an allelopathic crop that can alleviate the obstacles to Continuous Cropping of vegetable crops. Diallyl disulfide (DADS), one of the most important allelochemicals in garlic, promotes tomato root growth. Therefore, the global transcriptome profiles of DADS-treated tomato roots over time were investigated to reveal the potential growth-promoting mechanisms. We detected 1828, 1296 and 1190 differentially expressed genes (DEGs) in the 4, 24 and 48 h samples, respectively. Most DEGs involved in assimilatory sulfate reduction and glutathione metabolism were up-regulated after short-term (4 h) DADS treatment. In addition, increased activity of defensive enzymes and up-regulation of six peroxidase genes were observed, suggesting that DADS could induce tomato resistance. In plant-pathogen interactions, DEGs related to calcium signaling were primarily inhibited, while those encoding pathogenesis-related proteins were primarily up-regulated. Although plant hormone synthesis and signal transduction were both significantly affected by DADS, the expression trends of the genes in these two pathways were conflicting. This research provides comprehensive information concerning the changes in the tomato root transcriptome affected by DADS and may help direct further studies on DADS-responsive genes to enhance the current understanding of the mechanisms by which DADS alleviates the obstacles to Continuous Cropping.