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H K Parmentier - One of the best experts on this subject based on the ideXlab platform.

  • low molecular weight Cooperia oncophora antigens potential to discriminate between susceptible and resistant calves after infection
    International Journal for Parasitology, 1997
    Co-Authors: P M Van Diemen, A. Kloosterman, M G B Nieuwland, H W Ploeger, F W Rietveld, M Eysker, F N J Kooyman, H K Parmentier
    Abstract:

    Abstract The recognition of low molecular weight proteins by sera obtained during a single oral (primary) infection with 100 000 3rd-stage Cooperia oncophora larvae was studied in calves. Three groups of 6 or 7 calves were selected based on different egg excretion patterns. SDS-gel electrophoresis of adult Cooperia antigen under reducing conditions, followed by Western blotting, revealed that resistance of individual calves to C. oncophora might be related with antibody responses (42 days post infection) against at least 2 protein fragments (14–16 kDa and 27 kDa). The 14–16-kDa protein complex was bound, to some extent, by individual sera from all calves. The intensity of staining was negatively correlated with egg excretion on Day 42 p.i. Calves with high egg counts on Day 21 p.i. either did not or only weakly recognized the 27-kDa band. It has to be established whether the 14–16 kDa (or recombinant 14.2 kDa) provides a tool for immunodiagnostics and whether the 27-kDa fragment can help further unravel immune-mediated resistance to Cooperia .

  • recognition of low molecular weight Cooperia oncophora antigens after primary and trickle infection of calves with third stage infective larvae
    International Journal for Parasitology, 1996
    Co-Authors: P M Van Diemen, M G B Nieuwland, H W Ploeger, F W Rietveld, H K Parmentier
    Abstract:

    Abstract Recognition of low molecular protein fragments of adult Cooperia oncophora of calves was studied using sera obtained after a single oral (primary) infection with 100,000 infective 3rd-stage C. oncophora larvae, and after a 10-week period of trickle infection with moderate doses of these larvae. SDS-gel electrophoresis under reducing conditions, followed by western blotting, revealed that a complex of 14–16 kDa protein fragments, a 27 kDa and a 31.6 kDa band of Cooperia oncophora adult antigen were recognized by sera from calves both after primary and trickle infection. A 19 kDa and 30 kDa protein fragment were additionally bound by sera from calves after trickle infection. The data suggest that the systemic humoral immune response of calves to Cooperia oncophora after trickle infection is more pronounced (14–16, 27, 31.6 kDa) and extended (19, 30 kDa) than after primary infection with Cooperia .

  • systemic antibody responses of calves to low molecular weight Cooperia oncophora antigens
    Veterinary Parasitology, 1995
    Co-Authors: M G B Nieuwland, A. Kloosterman, H W Ploeger, H K Parmentier
    Abstract:

    Abstract The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12–15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.

  • low molecular weight Cooperia oncophora antigens characterization and humoral immune responses in calves mono infected with 100 000 infective larvae
    Veterinary Parasitology, 1995
    Co-Authors: H K Parmentier, M G B Nieuwland, H W Ploeger, F W Rietveld, P J E Souren, L A H Van Pinxteren, G De Vries Reilingh, A. Kloosterman
    Abstract:

    Abstract Characteristics of the humoral immune response of Cooperia oncophora -infected calves to low molecular weight antigens of C. oncophora were studied. Immunoblotting with sera obtained from calves 6 weeks after a single oral infection with 100 000 third-stage (L 3 ) C. oncophora larvae revealed several corresponding antigenic fragments between adult worms and the fourth-stage (L 4 ) larvae. No reactivity in the immune sera was found against the L 3 stage. A previously defined complex of low molecular weight proteins (12–15 kDa) was found on both L 4 and adult Cooperia stages, but not on the L 3 stage. C. oncophora adults differed from the L 4 larvae at the 31 32 and 37 kDa level. Several adult and L 4 proteins were bound by biotinylated Concanavalin A, as was also true for L 3 proteins. A 31 32 kDa glycoproprotein of adult worms was recognized by a monoclonal antibody with specificity for phosphorylcholine. Using monoclonal antibodies in ELISA and Western blotting, the serum antibody response of C. oncophora -infected calves to adult worm antigen was almost entirely IgG1. Binding of the IgG1 antibodies was restricted to a complex of reduced 12–15 kDa protein(s) and a 27 kDa fragment of adult worms. The data suggest that the systemic humoral immune response of calves during a primary infection with C. oncophora consists mainly of an IgG1 response, and is directed to a non-glycoslyated Cooperia protein (molecular weight estimated at 12–15 kDa under reducing conditions and 18 kDa under nonreducing conditions). This protein is probably present in both L 4 larvae and adults. Since it was not bound by immune sera from calves mono-infected with several other nematodes, the 12–15 kDa protein complex may represent a Cooperia -specific component that can be used for serodiagnosis.

A. Kloosterman - One of the best experts on this subject based on the ideXlab platform.

  • low molecular weight Cooperia oncophora antigens potential to discriminate between susceptible and resistant calves after infection
    International Journal for Parasitology, 1997
    Co-Authors: P M Van Diemen, A. Kloosterman, M G B Nieuwland, H W Ploeger, F W Rietveld, M Eysker, F N J Kooyman, H K Parmentier
    Abstract:

    Abstract The recognition of low molecular weight proteins by sera obtained during a single oral (primary) infection with 100 000 3rd-stage Cooperia oncophora larvae was studied in calves. Three groups of 6 or 7 calves were selected based on different egg excretion patterns. SDS-gel electrophoresis of adult Cooperia antigen under reducing conditions, followed by Western blotting, revealed that resistance of individual calves to C. oncophora might be related with antibody responses (42 days post infection) against at least 2 protein fragments (14–16 kDa and 27 kDa). The 14–16-kDa protein complex was bound, to some extent, by individual sera from all calves. The intensity of staining was negatively correlated with egg excretion on Day 42 p.i. Calves with high egg counts on Day 21 p.i. either did not or only weakly recognized the 27-kDa band. It has to be established whether the 14–16 kDa (or recombinant 14.2 kDa) provides a tool for immunodiagnostics and whether the 27-kDa fragment can help further unravel immune-mediated resistance to Cooperia .

  • systemic antibody responses of calves to low molecular weight Cooperia oncophora antigens
    Veterinary Parasitology, 1995
    Co-Authors: M G B Nieuwland, A. Kloosterman, H W Ploeger, H K Parmentier
    Abstract:

    Abstract The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12–15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.

  • low molecular weight Cooperia oncophora antigens characterization and humoral immune responses in calves mono infected with 100 000 infective larvae
    Veterinary Parasitology, 1995
    Co-Authors: H K Parmentier, M G B Nieuwland, H W Ploeger, F W Rietveld, P J E Souren, L A H Van Pinxteren, G De Vries Reilingh, A. Kloosterman
    Abstract:

    Abstract Characteristics of the humoral immune response of Cooperia oncophora -infected calves to low molecular weight antigens of C. oncophora were studied. Immunoblotting with sera obtained from calves 6 weeks after a single oral infection with 100 000 third-stage (L 3 ) C. oncophora larvae revealed several corresponding antigenic fragments between adult worms and the fourth-stage (L 4 ) larvae. No reactivity in the immune sera was found against the L 3 stage. A previously defined complex of low molecular weight proteins (12–15 kDa) was found on both L 4 and adult Cooperia stages, but not on the L 3 stage. C. oncophora adults differed from the L 4 larvae at the 31 32 and 37 kDa level. Several adult and L 4 proteins were bound by biotinylated Concanavalin A, as was also true for L 3 proteins. A 31 32 kDa glycoproprotein of adult worms was recognized by a monoclonal antibody with specificity for phosphorylcholine. Using monoclonal antibodies in ELISA and Western blotting, the serum antibody response of C. oncophora -infected calves to adult worm antigen was almost entirely IgG1. Binding of the IgG1 antibodies was restricted to a complex of reduced 12–15 kDa protein(s) and a 27 kDa fragment of adult worms. The data suggest that the systemic humoral immune response of calves during a primary infection with C. oncophora consists mainly of an IgG1 response, and is directed to a non-glycoslyated Cooperia protein (molecular weight estimated at 12–15 kDa under reducing conditions and 18 kDa under nonreducing conditions). This protein is probably present in both L 4 larvae and adults. Since it was not bound by immune sera from calves mono-infected with several other nematodes, the 12–15 kDa protein complex may represent a Cooperia -specific component that can be used for serodiagnosis.

  • Acquired immunity against Cooperia spp and Ostertagia spp in calves: Effect of level of exposure and timing of the midsummer increase.
    Veterinary parasitology, 1995
    Co-Authors: Harm W. Ploeger, A. Kloosterman, F W Rietveld
    Abstract:

    Abstract In two experiments, groups of calves were exposed to different levels and patterns of infection with Ostertagia spp. and Cooperia spp. The experimental design simulated the stereotypic pattern of herbage infestation, including both a normal and a delayed midsummer increase, under conditions of set-stocking. After this simulated ‘first grazing season’, calves were followed during the subsequent winter housing. At the end of that housing period some calves were collected with 100 000 L3 Cooperia spp. and 40 000 L3 Ostertagia spp. and slaughtered 23 days later. All previously infected calves were protected against the establishment of the challenge infection with Cooperia spp., but not against Ostertagia spp. For the latter a significant negative correlation was found between worm count and previous level of exposure to infection. During the simulated first grazing season, changes in the ratio of Cooperia to Ostertagia eggs in the faecal egg output and the genus-specific egg count were influenced by both the level of exposure and the timing of the midsummer increase. It is concluded that acquired immunity against both parasite genera develops depending on the level of exposure to infection during a first grazing season, and that delaying the midsummer increase results in a delay of the acquisition of an effective immunity as measured by faecal egg counts and the ratio of Cooperia to Ostertagia egg output.

  • Age resistance in calves to Ostertagia ostertagi and Cooperia oncophora
    Veterinary parasitology, 1991
    Co-Authors: A. Kloosterman, Harm W. Ploeger, K. Frankena
    Abstract:

    Abstract Calves were infected repeatedly during a period of 6 weeks with Ostertagia ostertagi and Cooperia oncophora, at an age of 3, 6 or 9 months. The inoculations were performed during three periods, February–March, May–June and August–September, to account for possible seasonal effects or effects of larval batches. Observations were done on faecal egg output, antibody titres and weight gains. Calves were slaughtered for post mortem examinations 9 weeks after the start of infections. The influence of age on worm populations and egg output was significant for C. oncophora but not for O. ostertagi. The effect of season or larval batch on worm populations was significant for O. ostertagi but not for C. oncophora. The correlations between worm numbers and several other parameters found for Cooperia were strongly indicative of a process of worm expulsion taking place at the stage of infection (9 weeks after the start of infections) when post mortem examinations were done. Such correlations were absent for Ostertagia. It is concluded that within the range of ages examined here (the range to which first season grazing calves belong), there is no influence of age on Ostertagia populations but a clear effect of age on Cooperia. This difference strongly influences the total faecal egg output of grazing calves and its interpretation.

H W Ploeger - One of the best experts on this subject based on the ideXlab platform.

  • b cells and antibody response in calves primary infected or re infected with Cooperia oncophora influence of priming dose and host responder types
    International Journal for Parasitology, 2003
    Co-Authors: K Kanobana, H W Ploeger, F N J Kooyman, Ad P Koets, N Bakker, Lonneke Vervelde
    Abstract:

    We investigated whether the generation of protective memory humoral immunity in Cooperia oncophora infected calves occurs in a dose-dependent way and whether it depends on the animal responder types. To this end, serum and mucus antibody responses were measured in animals primary-infected with 30000 or 100000 L3, treated with anthelmintics and subsequently challenged with 100000 L3. A detailed phenotypic and functional analysis of B cells was done in animals infected once or twice with 100,000 L3. Based on the similarity in parasitological variables of animals primed with 30000 or 100000 L3, we concluded that with these doses priming conferred protection in a dose-independent way. Upon challenge significant increases in Cooperia-specific serum and mucus IgG1 and IgA and total serum IgE titres were induced in primed animals in a dose-independent way. In contrast, intermediate and low responders differed in the onset of the production of Cooperia-specific serum IgG1. Furthermore, not only the onset but also the level of total serum IgE significantly differed between intermediate and low responders. Phenotypic and functional analysis of B lymphocytes revealed that (i). priming induced the generation of memory B cells which upon challenge readily differentiated into antibody secreting cells; (ii). sensitised B cells were more efficiently recruited to the intestinal effector sites; (iii). based on the expression of CD62L and CD86 two distinct B cell subpopulation could be differentiated. CD62L(+)CD86(-) B cells that were likely lymphocytes not yet activated and with an enhanced recirculation capacity, and CD62L(-)CD86(+) B cells that were activated B cells with a reduced recirculation ability; and finally (iv). the increased expression of CD86 and subsequent correlations with parameters of the T helper 2 immune response induced by C. oncophora, suggested that CD86- interactions are involved in the generation of protective immunity against Cooperia.

  • low molecular weight Cooperia oncophora antigens potential to discriminate between susceptible and resistant calves after infection
    International Journal for Parasitology, 1997
    Co-Authors: P M Van Diemen, A. Kloosterman, M G B Nieuwland, H W Ploeger, F W Rietveld, M Eysker, F N J Kooyman, H K Parmentier
    Abstract:

    Abstract The recognition of low molecular weight proteins by sera obtained during a single oral (primary) infection with 100 000 3rd-stage Cooperia oncophora larvae was studied in calves. Three groups of 6 or 7 calves were selected based on different egg excretion patterns. SDS-gel electrophoresis of adult Cooperia antigen under reducing conditions, followed by Western blotting, revealed that resistance of individual calves to C. oncophora might be related with antibody responses (42 days post infection) against at least 2 protein fragments (14–16 kDa and 27 kDa). The 14–16-kDa protein complex was bound, to some extent, by individual sera from all calves. The intensity of staining was negatively correlated with egg excretion on Day 42 p.i. Calves with high egg counts on Day 21 p.i. either did not or only weakly recognized the 27-kDa band. It has to be established whether the 14–16 kDa (or recombinant 14.2 kDa) provides a tool for immunodiagnostics and whether the 27-kDa fragment can help further unravel immune-mediated resistance to Cooperia .

  • recognition of low molecular weight Cooperia oncophora antigens after primary and trickle infection of calves with third stage infective larvae
    International Journal for Parasitology, 1996
    Co-Authors: P M Van Diemen, M G B Nieuwland, H W Ploeger, F W Rietveld, H K Parmentier
    Abstract:

    Abstract Recognition of low molecular protein fragments of adult Cooperia oncophora of calves was studied using sera obtained after a single oral (primary) infection with 100,000 infective 3rd-stage C. oncophora larvae, and after a 10-week period of trickle infection with moderate doses of these larvae. SDS-gel electrophoresis under reducing conditions, followed by western blotting, revealed that a complex of 14–16 kDa protein fragments, a 27 kDa and a 31.6 kDa band of Cooperia oncophora adult antigen were recognized by sera from calves both after primary and trickle infection. A 19 kDa and 30 kDa protein fragment were additionally bound by sera from calves after trickle infection. The data suggest that the systemic humoral immune response of calves to Cooperia oncophora after trickle infection is more pronounced (14–16, 27, 31.6 kDa) and extended (19, 30 kDa) than after primary infection with Cooperia .

  • systemic antibody responses of calves to low molecular weight Cooperia oncophora antigens
    Veterinary Parasitology, 1995
    Co-Authors: M G B Nieuwland, A. Kloosterman, H W Ploeger, H K Parmentier
    Abstract:

    Abstract The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12–15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.

  • low molecular weight Cooperia oncophora antigens characterization and humoral immune responses in calves mono infected with 100 000 infective larvae
    Veterinary Parasitology, 1995
    Co-Authors: H K Parmentier, M G B Nieuwland, H W Ploeger, F W Rietveld, P J E Souren, L A H Van Pinxteren, G De Vries Reilingh, A. Kloosterman
    Abstract:

    Abstract Characteristics of the humoral immune response of Cooperia oncophora -infected calves to low molecular weight antigens of C. oncophora were studied. Immunoblotting with sera obtained from calves 6 weeks after a single oral infection with 100 000 third-stage (L 3 ) C. oncophora larvae revealed several corresponding antigenic fragments between adult worms and the fourth-stage (L 4 ) larvae. No reactivity in the immune sera was found against the L 3 stage. A previously defined complex of low molecular weight proteins (12–15 kDa) was found on both L 4 and adult Cooperia stages, but not on the L 3 stage. C. oncophora adults differed from the L 4 larvae at the 31 32 and 37 kDa level. Several adult and L 4 proteins were bound by biotinylated Concanavalin A, as was also true for L 3 proteins. A 31 32 kDa glycoproprotein of adult worms was recognized by a monoclonal antibody with specificity for phosphorylcholine. Using monoclonal antibodies in ELISA and Western blotting, the serum antibody response of C. oncophora -infected calves to adult worm antigen was almost entirely IgG1. Binding of the IgG1 antibodies was restricted to a complex of reduced 12–15 kDa protein(s) and a 27 kDa fragment of adult worms. The data suggest that the systemic humoral immune response of calves during a primary infection with C. oncophora consists mainly of an IgG1 response, and is directed to a non-glycoslyated Cooperia protein (molecular weight estimated at 12–15 kDa under reducing conditions and 18 kDa under nonreducing conditions). This protein is probably present in both L 4 larvae and adults. Since it was not bound by immune sera from calves mono-infected with several other nematodes, the 12–15 kDa protein complex may represent a Cooperia -specific component that can be used for serodiagnosis.

M G B Nieuwland - One of the best experts on this subject based on the ideXlab platform.

  • low molecular weight Cooperia oncophora antigens potential to discriminate between susceptible and resistant calves after infection
    International Journal for Parasitology, 1997
    Co-Authors: P M Van Diemen, A. Kloosterman, M G B Nieuwland, H W Ploeger, F W Rietveld, M Eysker, F N J Kooyman, H K Parmentier
    Abstract:

    Abstract The recognition of low molecular weight proteins by sera obtained during a single oral (primary) infection with 100 000 3rd-stage Cooperia oncophora larvae was studied in calves. Three groups of 6 or 7 calves were selected based on different egg excretion patterns. SDS-gel electrophoresis of adult Cooperia antigen under reducing conditions, followed by Western blotting, revealed that resistance of individual calves to C. oncophora might be related with antibody responses (42 days post infection) against at least 2 protein fragments (14–16 kDa and 27 kDa). The 14–16-kDa protein complex was bound, to some extent, by individual sera from all calves. The intensity of staining was negatively correlated with egg excretion on Day 42 p.i. Calves with high egg counts on Day 21 p.i. either did not or only weakly recognized the 27-kDa band. It has to be established whether the 14–16 kDa (or recombinant 14.2 kDa) provides a tool for immunodiagnostics and whether the 27-kDa fragment can help further unravel immune-mediated resistance to Cooperia .

  • recognition of low molecular weight Cooperia oncophora antigens after primary and trickle infection of calves with third stage infective larvae
    International Journal for Parasitology, 1996
    Co-Authors: P M Van Diemen, M G B Nieuwland, H W Ploeger, F W Rietveld, H K Parmentier
    Abstract:

    Abstract Recognition of low molecular protein fragments of adult Cooperia oncophora of calves was studied using sera obtained after a single oral (primary) infection with 100,000 infective 3rd-stage C. oncophora larvae, and after a 10-week period of trickle infection with moderate doses of these larvae. SDS-gel electrophoresis under reducing conditions, followed by western blotting, revealed that a complex of 14–16 kDa protein fragments, a 27 kDa and a 31.6 kDa band of Cooperia oncophora adult antigen were recognized by sera from calves both after primary and trickle infection. A 19 kDa and 30 kDa protein fragment were additionally bound by sera from calves after trickle infection. The data suggest that the systemic humoral immune response of calves to Cooperia oncophora after trickle infection is more pronounced (14–16, 27, 31.6 kDa) and extended (19, 30 kDa) than after primary infection with Cooperia .

  • systemic antibody responses of calves to low molecular weight Cooperia oncophora antigens
    Veterinary Parasitology, 1995
    Co-Authors: M G B Nieuwland, A. Kloosterman, H W Ploeger, H K Parmentier
    Abstract:

    Abstract The systemic antibody responses to adult Cooperia oncophora antigen were studied using sera obtained from calves during a 6-week period following a single oral infection with either 20 000 or 100 000 third-stage C. oncophora larvae. Dose dependent increasing titres of IgG binding complete adult Cooperia antigen were found in the sera of Cooperia-infected calves. SDS-gel electrophoresis under reducing conditions, followed by Western blotting, revealed that the increase of IgG binding Cooperia antigens could be attributed mainly to specific binding of IgG to a complex of 12–15 kDa protein fragments of Cooperia adult antigen. This protein may represent a Cooperia oncophora-specific component that can be used for sero-diagnosis.

  • low molecular weight Cooperia oncophora antigens characterization and humoral immune responses in calves mono infected with 100 000 infective larvae
    Veterinary Parasitology, 1995
    Co-Authors: H K Parmentier, M G B Nieuwland, H W Ploeger, F W Rietveld, P J E Souren, L A H Van Pinxteren, G De Vries Reilingh, A. Kloosterman
    Abstract:

    Abstract Characteristics of the humoral immune response of Cooperia oncophora -infected calves to low molecular weight antigens of C. oncophora were studied. Immunoblotting with sera obtained from calves 6 weeks after a single oral infection with 100 000 third-stage (L 3 ) C. oncophora larvae revealed several corresponding antigenic fragments between adult worms and the fourth-stage (L 4 ) larvae. No reactivity in the immune sera was found against the L 3 stage. A previously defined complex of low molecular weight proteins (12–15 kDa) was found on both L 4 and adult Cooperia stages, but not on the L 3 stage. C. oncophora adults differed from the L 4 larvae at the 31 32 and 37 kDa level. Several adult and L 4 proteins were bound by biotinylated Concanavalin A, as was also true for L 3 proteins. A 31 32 kDa glycoproprotein of adult worms was recognized by a monoclonal antibody with specificity for phosphorylcholine. Using monoclonal antibodies in ELISA and Western blotting, the serum antibody response of C. oncophora -infected calves to adult worm antigen was almost entirely IgG1. Binding of the IgG1 antibodies was restricted to a complex of reduced 12–15 kDa protein(s) and a 27 kDa fragment of adult worms. The data suggest that the systemic humoral immune response of calves during a primary infection with C. oncophora consists mainly of an IgG1 response, and is directed to a non-glycoslyated Cooperia protein (molecular weight estimated at 12–15 kDa under reducing conditions and 18 kDa under nonreducing conditions). This protein is probably present in both L 4 larvae and adults. Since it was not bound by immune sera from calves mono-infected with several other nematodes, the 12–15 kDa protein complex may represent a Cooperia -specific component that can be used for serodiagnosis.

Gilson Pereira De ,oliveira - One of the best experts on this subject based on the ideXlab platform.

  • Fauna helmintológica de ovinos provenientes da microrregião de Jaboticabal, estado de São Paulo, Brasil
    Univ Federal Santa Maria, 2014
    Co-Authors: Maciel, Willian Giquelin, Felippelli Gustavo, Zanetti Lopes, Welber Daniel, Pires Teixeira, Weslen Fabricio, Cruz, Breno Cayeiro, Santos, Thais Rabelo Dos, Buzzulini Carolina, Favero Flavia, Gomes, Lucas Costa, Gilson Pereira De ,oliveira
    Abstract:

    This study evaluated the prevalence and counting parasitism of different species of helminths of sheep from the micro-region of Jaboticabal of Sao Paulo state. For this, 66 animals naturally infected, four to 36 months of age, raised in pasture, were selected. The results of necropsy revealed the presence of seven genera and 12 species with the following prevalence and mean count: Haemonchus contortus: 100.0% (2947.2); Trichostrongylus colubriformis: 90.9% (3048.8); Cooperia curticei: 56.0% (256.5); Oesophagostomum columbianum: 48.4% (36.0); Cooperia punctata: 30.3% (94.5); Trichostrongylus axei: 22.7% (26.5); Strongyloides papillosus: 19.6% (83.0), Haemonchus contortus (L4): 7.5% (17.2), Cooperia pectinata: 10.6% (12.9), Trichuris ovis: 10.6 % (0.6); Cooperia spatulata 4.5% (0.3); Capillaria bovis: 4.5% (0.1). The mean parasitism of helminthswas 6524.7 per animal. Haemonchus contortus (adults and L4) and Trichostrongylus colubriformis corresponded to 45.4% and 46.7% of the average worm burden totally, respectively. Based in the results obtained in this study, can be concluded that the two most abundant species of helminths and important, the micro-region of Jaboticabal are Trichostrongylus colubriformis and Haemonchus contortus, and these two species amounted to 92.1% of the distribution percentage of helminths collected from all animals. These results demonstrate the importance of conducting a counts of eggs per gram of feces (EPG) in the herds of this region when FAMACHA is used on a particular property, since this method control does not allow to diagnostic the damage/clinical signs in animals infected by T. colubriformis, because this specie does not have hematophagism habit on animals

  • Fauna helmintológica de ovinos provenientes da microrregião de Jaboticabal, estado de São Paulo, Brasil
    Universidade Federal de Santa Maria, 2014
    Co-Authors: Willian Giquelin Maciel, Gustavo Felippelli, Welber Daniel Zanetti Lopes, Weslen Fabricio Pires Teixeira, Breno Cayeiro Cruz, Thais Rabelo Dos Santos, Carolina Buzzulini, Flavia Favero, Lucas Costa Gomes, Gilson Pereira De ,oliveira
    Abstract:

    Avaliou-se a prevalência e a contagem parasitária das diferentes espécies de helmintos de ovinos provenientes da microrregião de Jaboticabal, região Noroeste de São Paulo. Para tanto, foram utilizados 66 animais naturalmente infectados, de quatro a 36 meses de idade, criados em regime extensivo. Os resultados necroscópicos revelaram a presença de sete gêneros e 12 espécies, com a seguinte prevalência e a média de parasitismo: Haemonchus contortus: 100,0% (2947,2); Trichostrongylus colubriformis: 90,9% (3048,8); Cooperia curticei: 56,0% (256,5); Oesophagostomum columbianum: 48,4% (36,0); Cooperia punctata: 30,3% (94,5); Trichostrongylus axei: 22,7% (26,5); Strongyloides papillosusi: 19,6% (83,0); Haemonchus contortus (L4): 7,5% (17,2); Cooperia pectinatai: 10,6% (12,9); Trichuris ovis: 10,6% (0,6); Cooperia spatulata 4,5% (0,3); Capillaria bovis: 4,5% (0,1). A carga parasitária média foi de 6.524,7 helmintos por animal. Haemonchus contortus (Adultos e L4) e Trichostrongylus colubriformis corresponderam a 45,4% e 46,7% da carga parasitária média total, respectivamente. Pode-se concluir que as duas espécies de helmintos mais abundantes e importantes da microrregião de Jaboticabal/São Paulo foram Trichostrongylus colubriformis e Haemonchus contortus, sendo que essas duas espécies perfizeram 92,1% da distribuição percentual dos helmintos recolhidos de todos os animais. Tais resultados demonstram a importância em se realizar um monitoramento das contagens de ovos por grama de fezes (OPG) dos rebanhos desta região, quando o método FAMACHA for empregado em uma determinada propriedade, uma vez que este método de controle, geralmente, não permite diagnosticar os danos/sinais clínicos desencadeados nos animais pelo T. colubriformis, em função de essa espécie não possuir hábito de hematofagismo sobre os hospedeiros

  • Prevalence and intensity of infection by helminths in bovines from the dairy basin of São Carlos, SP, Brazil
    'Embrapa Agroindustria Tropical', 2014
    Co-Authors: Gilson Pereira De ,oliveira, Matsumoto Takashi
    Abstract:

    Para estudo preliminar de helmintos de bovinos da bacia leiteira de São Carlos, SP, que abrange os municípios de São Carlos, Ribeirão Bonito e Dourado, foram necropsiados 74 animais mestiços Holandês-Zebu, na faixa etária de 8 a 20 meses. Do material coletado do tubo digestivo, pulmões e pâncreas, foram observadas as espécies de helmintos com as seguintes prevalências e intensidades médias de infecção: Haemonchus contortus, 100% e 988,06; Haemonchus similis 43,24% e 50,01; Trichostrongylus axei, 24,32% e 16,00; Cooperia punctata 100% e 3649,45 ;Cooperia pectinata 44,59% e 436,70; Bunostomum phlebotomum, 9,45% e 3,17; Oesophagostomum radiatum, 86,48% e  138,35; Trichuris discolor, 21,62% e 7,44;Agriostomum wryburgi, 2,70% e 0,22; Dictyocaulus viviparus 13,51% e 51,62; Eurytrema coelomatlum, 14,86% e 27,67.On a preliminary study of bovine helminths on dairy herds of São Carlos, SP, Brazil, that includes the counties of São Carlos, Ribeirão Bonito and Dourado, 74 holstein-zebu mixed breeding animals, 8 to 20-month old, were necropsied. From the gastrointestinal tract, lung and pancreas material was collected, and the prevalence of infection and average worm burden of species of helminths observed were: Haemonchus contortus 100% and 988.06 Haemonchus similis, 43.24% and 50.01; Trichostrongylus axei, 24.32% and 16.00; Cooperia punctata 100% and 3,649.45; Cooperia pectinata, 44.59% and 436.70; Bunostomum phlebotomum, 9.45% and 3.17; Oesophagostonum radiatum, 86,48% and 138.35; Trichuris discolor, 21.62% and 7.44; Agriostomum wryburgi, 2.70% and 0.22; Dictyocaulus viviparus, 13.51% and 51.62; Eurytrema coelomatium, 14.86% and 27.67

  • Helminth fauna of bovines from the Central-Western region, Minas Gerais State, Brazil
    Universidade Federal de Santa Maria (UFSM), 2010
    Co-Authors: Santos, Thais Rabelo Dos, Gilson Pereira De ,oliveira, Lopes, Welber Daniel Zanetti, Buzulini Carolina, Borges, Fernando De Almeida, Sakamoto, Cláudio Alessandro Massamitsu, Lima, Roberto Cesar De Araújo, Alvimar Jose Da ,costa
    Abstract:

    A prevalência e a intensidade de parasitismo por diferentes espécies de helmintos foram estudadas em bovinos da microrregião de Formiga, região Centro-oeste de Minas Gerais. Para tanto, foram necropsiados 76 bovinos naturalmente infectados, machos e fêmeas, SRD (sem raça definida) e de oito a 12 meses de idade. Os resultados necroscópicos revelaram a presença de nove gêneros e 16 espécies de helmintos, com a seguinte prevalência e média de intensidade de infecção: Haemonchus placei (100,0%; 3895,5); Haemonchus similis (29,0%; 159,6); Cooperia punctata (100,0%; 5595,0); Cooperia spatulata (32,9%; 137,8); Cooperia pectinata (34,2%; 1010,5); Trichostrongylus axei (69,7%; 239,2); Trichostrongylus colubriformis (10,5%; 10,8); Trichostrongylus longyspicularis (2,6%; 0,5); Ostertagia ostertagi (2,6%; 3,1); Ostertagia lyrata (2,6%; 1,5); Ostertagia trifurcata (1,3%; 0,3); Oesophagostomum radiatum (94,7%; 470,9); Trichuris discolor (47,4%; 32,5); Strongyloides papillosus (1,3%; 0,1); Capillaria bovis (9,2%; 10) e Bunostomum phlebotomum (2,6%; 0,3). A carga parasitária média foi de 11.558,5 helmintos por animal. Dos 76 bovinos necropsiados, 92,1% estavam infectados por três a sete espécies de helmintos. Apenas 7,9% dos hospedeiros mostravam-se parasitados por oito espécies diferentes de helmintos. Este estudo mostra o primeiro relato das espécies Ostertagia lyrata e Ostertagia trifurcata no Estado de Minas Gerais. É importante ressaltar que, no presente trabalho, por meio da identificação dos helmintos colhidos nas necropsias, foi possível observar que está ocorrendo uma inversão na intensidade parasitária média, com uma diminuição no número de Cooperia e um aumento nos valores de Haemonchus, em comparação com os valores relatados na literatura.Seventy-six naturally infected bovines, males and females of mixed breed, aged 8 to 12 months-old, were necropsied. The results of necropsy revealed the presence of 9 helminth genera and 16 species, with the following prevalence and mean infection intensity: Haemonchus placei (100.0%; 3895.5); Haemonchus similis (29.0%; 159.6); Cooperia punctata (100.0%; 5595.0); Cooperia spatulata (32.9%; 137.8); Cooperia pectinata (34.2%; 1010.5); Trichostrongylus axei (69.7%; 239.2); Trichostrongylus colubriformis (10.5%; 10.8); Trichostrongylus longyspicularis (2.6%; 0.5); Ostertagia ostertagi (2.6%; 3.1); Ostertagia lyrata (2.6%; 1.5); Ostertagia trifurcata (1.3%; 0.3); Oesophagostomum radiatum (94.7%; 470.9); Trichuris discolor (47.4%; 32.5); Strongyloides papillosus (1.3%; 0.1); Capillaria bovis (9.2%; 1.0) and Bunostomum phlebotomum (2.6%; 0.3). The mean parasitic load was 11,558.5 helminths per bovine. of the 76 necropsied bovine, 92.1% were infected by 3 to 7 helminth species. Only 7.9% of hosts were parasitized by 8 different helminth species. This study includes the first report of the species Ostertagia lyrata and Ostertagia trifurcata in Minas Gerais state. It should be emphasized that while identifying the helminths collected during necropsy in the present work, observation revealed that an inversion in the mean parasitic intensity is occurring, showing diminishing numbers of Cooperia and an increase in Haemonchus compared to the values reported in the literature

  • Helminth fauna of bovines from the Central-Western region, Minas Gerais State, Brazil
    Universidade Federal de Santa Maria, 2010
    Co-Authors: Santos, Thais Rabelo Dos, Gilson Pereira De ,oliveira, Lopes, Welber Daniel Zanetti, Borges, Fernando De Almeida, Sakamoto, Cláudio Alessandro Massamitsu, Lima, Roberto Cesar De Araújo, Alvimar Jose Da ,costa
    Abstract:

    Seventy-six naturally infected bovines, males and females of mixed breed, aged 8 to 12 months-old, were necropsied. The results of necropsy revealed the presence of 9 helminth genera and 16 species, with the following prevalence and mean infection intensity: Haemonchus placei (100.0%; 3895.5); Haemonchus similis (29.0%; 159.6); Cooperia punctata (100.0%; 5595.0); Cooperia spatulata (32.9%; 137.8); Cooperia pectinata (34.2%; 1010.5); Trichostrongylus axei (69.7%; 239.2); Trichostrongylus colubriformis (10.5%; 10.8); Trichostrongylus longyspicularis (2.6%; 0.5); Ostertagia ostertagi (2.6%; 3.1); Ostertagia lyrata (2.6%; 1.5); Ostertagia trifurcata (1.3%; 0.3); Oesophagostomum radiatum (94.7%; 470.9); Trichuris discolor (47.4%; 32.5); Strongyloides papillosus (1.3%; 0.1); Capillaria bovis (9.2%; 1.0) and Bunostomum phlebotomum (2.6%; 0.3). The mean parasitic load was 11,558.5 helminths per bovine. Of the 76 necropsied bovine, 92.1% were infected by 3 to 7 helminth species. Only 7.9% of hosts were parasitized by 8 different helminth species. This study includes the first report of the species Ostertagia lyrata and Ostertagia trifurcata in Minas Gerais state. It should be emphasized that while identifying the helminths collected during necropsy in the present work, observation revealed that an inversion in the mean parasitic intensity is occurring, showing diminishing numbers of Cooperia and an increase in Haemonchus compared to the values reported in the literature