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Xian-le Yang - One of the best experts on this subject based on the ideXlab platform.
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Effect of Copper Sulfate on Bdellovibrio growth and bacteriolytic activity towards gibel carp-pathogenic Aeromonas hydrophila.
Canadian Journal of Microbiology, 2018Co-Authors: Haipeng Cao, Yang Yibin, Xian-le YangAbstract:The use of bdellovibrios has been regarded as an alternative to control multidrug-resistant pathogens and fish bacteriosis. However, scarce information is available on the potential of bdellovibrios in the presence of Copper Sulfate, which is an algicide widely used to treat cyanobacterial blooms in aquaculture. In the present study, the effects of Copper Sulfate at sublethal and lethal levels (0.1 and 1.0 mg·L−1) on Bdellovibrio sp. strain BDF-H16 were evaluated. The growth of Bdellovibrio sp. strain BDF-H16 was significantly promoted by both concentrations of Copper Sulfate, but less so by the lethal concentration. The bacteriolysis of gibel carp-pathogenic Aeromonas hydrophila by Bdellovibrio sp. strain BDF-H16 was also stimulated by Copper Sulfate in both solid and liquid media. However, Bdellovibrio sp. strain BDF-H16 with 0.1 mg·L−1 Copper Sulfate clearly inhibited infection of gibel carps by A. hydrophila better than Bdellovibrio sp. strain BDF-H16 with 1.0 mg·L−1 Copper Sulfate did.
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Analysis of Saprolegnia parasitica Transcriptome following Treatment with Copper Sulfate
PLOS ONE, 2016Co-Authors: Cheng Junming, Qi Sun, Hai-lan Yuan, Liang Nan, Wenhong Fang, Li Haoran, Xian-le YangAbstract:Background Massive infection caused by oomycete fungus Saprolegnia parasitica is detrimental to freshwater fish. Recently, we showed that Copper Sulfate demonstrated good efficacy for controlling S. parasitica infection in grass carp. In this study, we investigated the mechanism of inhibition of S. parasitica growth by Copper Sulfate by analyzing the transcriptome of Copper Sulfate—treated S. parasitica. To examine the mechanism of Copper Sulfate inhibiting S. parasitica, we utilized RNA-seq technology to compare differential gene expression in S. parasitica treated with or without Copper Sulfate. Results The total mapped rates of the reads with the reference genome were 90.50% in the control group and 73.50% in the experimental group. In the control group, annotated splice junctions, partial novel splice junctions and complete novel splice junctions were about 83%, 3% and 14%, respectively. In the treatment group, the corresponding values were about 75%, 6% and 19%. Following Copper Sulfate treatment, a total 310 genes were markedly upregulated and 556 genes were markedly downregulated in S. parasitica. Material metabolism related GO terms including cofactor binding (33 genes), 1,3-beta-D-glucan synthase complex (4 genes), carboxylic acid metabolic process (40 genes) were the most significantly enriched. KEGG pathway analysis also determined that the metabolism-related biological pathways were significantly enriched, including the metabolic pathways (98 genes), biosynthesis of secondary metabolites pathways (42 genes), fatty acid metabolism (13 genes), phenylalanine metabolism (7 genes), starch and sucrose metabolism pathway (12 genes). The qRT-PCR results were largely consistent with the RNA-Seq results. Conclusion Our results indicate that Copper Sulfate inhibits S. parasitica growth by affecting multiple biological functions, including protein synthesis, energy biogenesis, and metabolism.
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The Efficacy of Copper Sulfate in Controlling Infection of Saprolegnia parasitica
Journal of The World Aquaculture Society, 2014Co-Authors: Qi Sun, Xian-le YangAbstract:Saprolegnia parasitica is a severe fish pathogen that causes important economic losses worldwide. Copper is an important additive in the aquaculture industry for control of algal growth, ectoparasites, and fungal disease. However, at present no data is available on the specific interaction of Copper Sulfate with oomycete. In our study, the efficacy of Copper Sulfate on the mycelium and zoospore of S. parasitica was assessed in vitro, and S. parasitica infection experiment was conducted to assess its performance in vivo. The results indicated that Copper Sulfate at ≥0.5 mg/L inhibited the growth of mycelium, no primary zoospores were released at ≥1.0 mg/L. Additionally, 0.5 mg/L Copper Sulfate could reduce the infection rate of S. parasitica in the grass carp, Ctenopharyngodon idellus. This study demonstrates the good efficacy of Copper Sulfate on the control of S. parasitica infection in grass carps, and suggests that Copper Sulfate could be used as a drug additive to control the S. parasitica infection in the aquaculture industry.
Claude E. Boyd - One of the best experts on this subject based on the ideXlab platform.
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Slow‐release Coated Copper Sulfate as an Algicide for Aquaculture
Journal of The World Aquaculture Society, 2016Co-Authors: Rawee Viriyatum, Claude E. BoydAbstract:A coated Copper Sulfate algicide designed for controlled release of Copper was evaluated for its effectiveness in controlling phytoplankton in hybrid catfish, ♀Ictalurus punctatus × ♂Ictalurus furcatus, ponds. Copper concentrations were greater in ponds receiving weekly treatments with Copper Sulfate crystals than in ponds in which the coated Copper Sulfate was suspended in porous bags and left in ponds during the study. However, the coated Copper Sulfate treatment provided a similar degree of phytoplankton control for a period of about 4 mo. Copper additions did not negatively affect catfish survival, production, or feed conversion in either the Copper Sulfate crystal treatment or in the coated Copper Sulfate treatment as compared with the control (P > 0.05). Flavor scores for fish did not differ between control and treatments (P > 0.05). The coated Copper Sulfate appeared to be a potentially effective method for controlling phytoplankton in aquaculture ponds. It would be easier to apply and require fewer applications, and the coated Copper algicide would not present a fish toxicity issue that can arise from high Copper concentration immediately following Copper Sulfate crystal treatment.
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Copper Concentrations in Channel Catfish Ictalurus punctatus Ponds Treated with Copper Sulfate
Journal of The World Aquaculture Society, 2004Co-Authors: Aaron A. Mcnevin, Claude E. BoydAbstract:Copper Sulfate (CuSO45H2O) is used to reduce the abundance of blue-green algae and combat off-flavor in channel catfish culture. Copper Sulfate usually is applied at a concentration of one-one hundredth of the total alkalinity. A study was performed at the Auburn University Fisheries Research Unit to determine the duration of elevated Copper (Cu) concentration following Copper Sulfate applications. Two alkalinity treatments, 20-40 mg/L and 110-130 mg/L (as CaCO3), were examined. Copper Sulfate was applied biweekly for 14 wk at 03 mg/L for the low alkalinity treatment and 1.2 mg/L for the high alkalinity treatment. Total Copper concentrations in pond waters declined to the background level by 48-h post treatment. In addition, total Copper concentrations were determined in waters of 38 catfish production ponds located in west central Alabama. The mean and standard deviation were 0.0092 ± 0.0087 mg Cu/L. Copper quickly precipitates from the water or is absorbed by sediments following Copper Sulfate treatment. Although concentrations of Copper in pond waters increase immediately following Copper Sulfate treatment, they rapidly decrease and seldom exceed the United States Environmental Protection Agency's National Recommended Water Quality Criteria for Priority Toxic Pollutants of 0.013-mg Cu/L. Findings of this study suggest that Copper Sulfate treatment will not contaminate effluent from catfish ponds because of the short time that applied Copper remains in the water column. Furthermore, the most frequent applications of Copper Sulfate occur in late summer months when rainfall is minimal and pond overflow is rare.
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Comparative evaluation of the solubility and algal toxicity of Copper Sulfate and chelated Copper
Aquaculture, 1993Co-Authors: Kiyoshi Masuda, Claude E. BoydAbstract:Abstract Concentrations of total Copper in solutions of several pH values and concentrations of total alkalinity and total hardness were always higher when Copper was applied as chelated Copper than when Copper was added in equal amounts as Copper Sulfate. Equilibrium calculations revealed that the increase in Copper concentrations following chelated Copper treatment resulted from an increase in the concentration of complexed Copper; cupric ion (Cu2+) concentration was the same with both algicides. When Copper was applied to laboratory soil-water systems, Copper was lost from solution slightly faster when Copper Sulfate was the source of Copper than when chelated Copper was used. Results of this study suggest that somewhat larger and more frequent doses of Copper Sulfate should be just as effective as chelated Copper algicides.
Eleftherios Makris - One of the best experts on this subject based on the ideXlab platform.
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a Copper Sulfate and hydroxylysine treatment regimen for enhancing collagen cross linking and biomechanical properties in engineered neocartilage
The FASEB Journal, 2013Co-Authors: Regina F Macbarb, Donald J Responte, Eleftherios Makris, Jerry C. Hu, Kyriacos A AthanasiouAbstract:The objective of this study was to improve the biomechanical properties of engineered neotissues through promoting the development of collagen cross-links. It was hypothesized that supplementing medium with Copper Sulfate and the amino acid hydroxylysine would enhance the activity of lysyl oxidase enzyme to form collagen cross-links, increasing the strength and integrity of the neotissue. Neocartilage constructs were generated using a scaffoldless, self-assembling process and treated with Copper Sulfate and hydroxylysine, either alone or in combination, following a 2-factor, full-factorial study design. Following a 6-wk culture period, the biomechanical and biochemical properties of the constructs were measured. Results found Copper Sulfate to significantly increase pyridinoline (PYR) cross-links in all Copper Sulfate-containing groups over controls. When Copper Sulfate and hydroxylysine were combined, the result was synergistic, with a 10-fold increase in PYR content over controls. This increase in PYR cross-links manifested in a 3.3-fold significant increase in the tensile properties of the Copper Sulfate + hydroxylysine group. In addition, an 123% increase over control values was detected in the Copper Sulfate group in terms of the aggregate modulus. These data elucidate the role of Copper Sulfate and hydroxylysine toward improving the biomechanical properties of neotissues through collagen cross-linking enhancement.—Makris, E. A., MacBarb, R. F., Responte, D. J., Hu, J. C., Athanasiou, K. A. A Copper Sulfate and hydroxylysine treatment regimen for enhancing collagen cross-linking and biomechanical properties in engineered neocartilage.
Qi Sun - One of the best experts on this subject based on the ideXlab platform.
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Analysis of Saprolegnia parasitica Transcriptome following Treatment with Copper Sulfate
PLOS ONE, 2016Co-Authors: Cheng Junming, Qi Sun, Hai-lan Yuan, Liang Nan, Wenhong Fang, Li Haoran, Xian-le YangAbstract:Background Massive infection caused by oomycete fungus Saprolegnia parasitica is detrimental to freshwater fish. Recently, we showed that Copper Sulfate demonstrated good efficacy for controlling S. parasitica infection in grass carp. In this study, we investigated the mechanism of inhibition of S. parasitica growth by Copper Sulfate by analyzing the transcriptome of Copper Sulfate—treated S. parasitica. To examine the mechanism of Copper Sulfate inhibiting S. parasitica, we utilized RNA-seq technology to compare differential gene expression in S. parasitica treated with or without Copper Sulfate. Results The total mapped rates of the reads with the reference genome were 90.50% in the control group and 73.50% in the experimental group. In the control group, annotated splice junctions, partial novel splice junctions and complete novel splice junctions were about 83%, 3% and 14%, respectively. In the treatment group, the corresponding values were about 75%, 6% and 19%. Following Copper Sulfate treatment, a total 310 genes were markedly upregulated and 556 genes were markedly downregulated in S. parasitica. Material metabolism related GO terms including cofactor binding (33 genes), 1,3-beta-D-glucan synthase complex (4 genes), carboxylic acid metabolic process (40 genes) were the most significantly enriched. KEGG pathway analysis also determined that the metabolism-related biological pathways were significantly enriched, including the metabolic pathways (98 genes), biosynthesis of secondary metabolites pathways (42 genes), fatty acid metabolism (13 genes), phenylalanine metabolism (7 genes), starch and sucrose metabolism pathway (12 genes). The qRT-PCR results were largely consistent with the RNA-Seq results. Conclusion Our results indicate that Copper Sulfate inhibits S. parasitica growth by affecting multiple biological functions, including protein synthesis, energy biogenesis, and metabolism.
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The Efficacy of Copper Sulfate in Controlling Infection of Saprolegnia parasitica
Journal of The World Aquaculture Society, 2014Co-Authors: Qi Sun, Xian-le YangAbstract:Saprolegnia parasitica is a severe fish pathogen that causes important economic losses worldwide. Copper is an important additive in the aquaculture industry for control of algal growth, ectoparasites, and fungal disease. However, at present no data is available on the specific interaction of Copper Sulfate with oomycete. In our study, the efficacy of Copper Sulfate on the mycelium and zoospore of S. parasitica was assessed in vitro, and S. parasitica infection experiment was conducted to assess its performance in vivo. The results indicated that Copper Sulfate at ≥0.5 mg/L inhibited the growth of mycelium, no primary zoospores were released at ≥1.0 mg/L. Additionally, 0.5 mg/L Copper Sulfate could reduce the infection rate of S. parasitica in the grass carp, Ctenopharyngodon idellus. This study demonstrates the good efficacy of Copper Sulfate on the control of S. parasitica infection in grass carps, and suggests that Copper Sulfate could be used as a drug additive to control the S. parasitica infection in the aquaculture industry.
Kyriacos A Athanasiou - One of the best experts on this subject based on the ideXlab platform.
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a Copper Sulfate and hydroxylysine treatment regimen for enhancing collagen cross linking and biomechanical properties in engineered neocartilage
The FASEB Journal, 2013Co-Authors: Regina F Macbarb, Donald J Responte, Eleftherios Makris, Jerry C. Hu, Kyriacos A AthanasiouAbstract:The objective of this study was to improve the biomechanical properties of engineered neotissues through promoting the development of collagen cross-links. It was hypothesized that supplementing medium with Copper Sulfate and the amino acid hydroxylysine would enhance the activity of lysyl oxidase enzyme to form collagen cross-links, increasing the strength and integrity of the neotissue. Neocartilage constructs were generated using a scaffoldless, self-assembling process and treated with Copper Sulfate and hydroxylysine, either alone or in combination, following a 2-factor, full-factorial study design. Following a 6-wk culture period, the biomechanical and biochemical properties of the constructs were measured. Results found Copper Sulfate to significantly increase pyridinoline (PYR) cross-links in all Copper Sulfate-containing groups over controls. When Copper Sulfate and hydroxylysine were combined, the result was synergistic, with a 10-fold increase in PYR content over controls. This increase in PYR cross-links manifested in a 3.3-fold significant increase in the tensile properties of the Copper Sulfate + hydroxylysine group. In addition, an 123% increase over control values was detected in the Copper Sulfate group in terms of the aggregate modulus. These data elucidate the role of Copper Sulfate and hydroxylysine toward improving the biomechanical properties of neotissues through collagen cross-linking enhancement.—Makris, E. A., MacBarb, R. F., Responte, D. J., Hu, J. C., Athanasiou, K. A. A Copper Sulfate and hydroxylysine treatment regimen for enhancing collagen cross-linking and biomechanical properties in engineered neocartilage.