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Ronald D Cave - One of the best experts on this subject based on the ideXlab platform.

  • efecto del hongo isaria fumosorosea wize sobre la herbivoria por los adultos del escarabajo de margen amarillo microtheca ochroloma stal coleoptera chrysomelidae
    Ceiba: A Scientific and Technical Journal, 2017
    Co-Authors: Cecilia Gamez Herrera, Angie Nino, Pasco B. Avery, Ronald D Cave
    Abstract:

    The yellowmargined leaf beetle, Microtheca ochroloma Stal (Coleoptera: Chrysomelidae), is a pest that causes economic losses in the production of crucifers (Brassicaceae) in Argentina and the southeastern USA. Biological control using the entomopathogenic fungus Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) is an option for suppressing pest populations. This study evaluated the effect of this fungus on herbivory by adult beetles when they were fed leaves treated with I. fumosorosea. Four concentrations (0.1, 0.5, 1.0, and 2.0 g) of the formulation PFR-97 20% WDG Apopka strain 97 per 100 ml of distilled water and a distilled water only control were evaluated. Two trials each with 10 bok choy plants per treatment were performed, and each plant was infested with six adult beetles. On the seventh day, leaf area consumed by the beetles was measured. In the first trial, plants applied with 1.0 and 2.0 g/100 ml suffered significantly less damage than the control. In the second trial, there was a significant difference between the control and concentration of 2.0 g/100 ml. The control had 3.7 and 2.0% more damage compared to the other treatments in the first and second trials, respectively.Ceiba, 2016. Volumen 54(2):118-126

  • infection and mortality of microtheca ochroloma coleoptera chrysomelidae by isaria fumosorosea hypocreales Cordycipitaceae under laboratory conditions
    Biocontrol Science and Technology, 2016
    Co-Authors: Cecil O Montemayor, Pasco B. Avery, Ronald D Cave
    Abstract:

    ABSTRACTMicrotheca ochroloma Stal, the yellowmargined leaf beetle, is a pest in crucifer crops during the late fall and winter months in Florida. On organic farms, it is difficult to control due to the restricted use of insecticides, in addition to the lack of specific natural enemies. The objective of this study was to evaluate a blastospore-formulated product of Isaria fumosorosea (PFR-97TM 20% WDG) against this beetle. In the first experiment, four of the beetle's life stages were treated with a suspension of 3 × 107 blastospores/ml. Mean corrected mortality of treated insects was significantly higher in 1st and 3rd instars than in the egg, pupal, and adult stages. Larvae infected by I. fumosorosea exhibited reduced growth and unsuccessful molting. The second experiment quantified mortality of first instars of M. ochroloma by four concentrations of PFR-97TM. Mean corrected larval infection/treatment was significantly (2.6 times) higher with a concentration of 4 g of product per 100 ml of water compared...

Hugo C Arredondobernal - One of the best experts on this subject based on the ideXlab platform.

  • selection of beauveria bassiana hypocreales Cordycipitaceae strains to control xyleborus affinis curculionidae scolytinae females
    PeerJ, 2020
    Co-Authors: Jesus E Castrejonantonio, Roberto Montesinosmatias, Patricia Tamezguerra, Maria Julissa Ekramos, Paul Misael Garzalopez, Hugo C Arredondobernal
    Abstract:

    Background Xyleborus affinis Eichhoff (Coleoptera: Curculionidae) is an ambrosia beetle reported to affect avocado trees (Persea americana Mill.). The use of the entomopathogenic fungus (EPF) Beauveria bassiana (Bals.-Criv.) Vuill. for ambrosia beetle control represents an alternative to insecticides. Methods This study was designed in two stages to select B. bassiana strains with potential to control X. affinis females. In the first stage, 19 B. bassiana Mexican strains from EPF collection, isolated from Coleoptera (CHE-CNRCB, http://www.gob.mx/senasica/documentos/coleccion-de-hongos-entomopatogenos), were tested. Analyses included radial growth rate, conidial yield, spore germination, and germ tube length. Results were analysed by Principal Component Analysis (PCA) to identify clusters within favourable growth phenotypes. For the second stage, 10 selected strains were re-analysed for virulence-related metabolic characteristic, including cell wall-bound cuticle-degrading enzymes-Pr1-like proteases and β-N-acetyl glucosaminidases (NAGase) chitinases, conidial hydrophobicity and monopolar germination parameters. A second PCA analysis was run for those virulence parameters analysed, and upon results strains CHE-CNRCB 44, 171, 431 and 485 were selected and tested against X. affinis females. Females were treated with a 1 × 108 conidia mL-1 suspension (recommended rate), using a Potter Tower. Results All strains showed insecticidal activity, inducing up to 58% mortality; about 30% dead beetles developed aerial mycelia (CHE-CNRCB 485) and the fastest mortality rate was t0 = 1.95 (CHE-CNRCB 44). Conclusion Since all selected strains showed virulence against X. affinis females, results indicated the possibility of selecting B. bassiana strains based on multiple metabolic attributes, as a preliminary test to perform bioassays against order-related target insects.

  • species clarification of isaria isolates used as biocontrol agents against diaphorina citri hemiptera liviidae in mexico
    Fungal Biology, 2016
    Co-Authors: Adrien Gallou, Maria G Sernadominguez, Angelica Maria Berlangapadilla, Miguel Angel Ayalazermeno, Marco A Mellinrosas, Roberto Montesinosmatias, Hugo C Arredondobernal
    Abstract:

    Entomopathogenic fungi belonging to the genus Isaria (Hypocreales: Cordycipitaceae) are promising candidates for microbial control of insect pests. Currently, the Mexican government is developing a biological control program based on extensive application of Isaria isolates against Diaphorina citri (Hemiptera: Liviidae), a vector of citrus huanglongbing disease. Previous research identified three promising Isaria isolates (CHE-CNRCB 303, 305, and 307; tentatively identified as Isaria fumosorosea) from Mexico. The goal of this work was to obtain a complete morphological and molecular characterization of these isolates. Comparative analysis of morphology established that the isolates showed similar characteristics to Isaria javanica. Multi-gene analysis confirmed the morphological identification by including the three isolates within the I. javanica clade. Additionally, this work demonstrated the misidentifications of three other Isaria isolates (CHE-CNRCB 310 and 324: I. javanica, formerly I. fumosorosea; CHE-CNRCB 393: I. fumosorosea, formerly Isaria farinosa), underlying the need for a full and correct characterization of an isolate before developing a biological control program. Finally, the inter-simple sequence repeat (ISSR) genotyping method revealed that the CHE-CNRCB 303, 305, and 307 isolates belong to three different genotypes. This result indicates that ISSR markers could be used as a tool to monitor their presence in field conditions.

Cabello, Marta Noemí - One of the best experts on this subject based on the ideXlab platform.

  • Cordyceps locustiphila (Hypocreales: Cordycipitaceae) infecting the grasshopper pest Tropidacris collaris (Orthoptera: Acridoidea: Romaleidae)
    2018
    Co-Authors: Pelizza, Sebastian A., Scattolini, María C., Bardi Cristian, Lange, Carlos Ernesto, Stenglein Sebastián, Cabello, Marta Noemí
    Abstract:

    Cordyceps locustiphila is described, illustrated, and compared with a previous finding. This species was collected infecting Tropidacris collaris in the Yabotí biosphere reserve, Misiones province, Argentina. Until now, this entomopathogenic fungus has not been reported affecting T. collaris, and it is the first report for Argentina. From the cordyceps-like stromata of C. locustiphila it was possible to isolate for the first time the acremonium-like anamorph of this fungus. The identity of both, anamorph and teleomorph, was determined by morphological and molecular taxonomic studies, which challenges the recent new combination into Beauveria

  • Cordyceps locustiphila (Hypocreales: Cordycipitaceae) infecting the grasshopper pest tropidacris collaris (orthoptera: Acridoidea: Romaleidae)
    'Schweizerbart', 2018
    Co-Authors: Pelizza, Sebastian Alberto, Bardi Cristian, Lange, Carlos Ernesto, Scattolini, María Celeste, Stenglein, Sebastian Alberto, Cabello, Marta Noemí
    Abstract:

    Cordyceps locustiphila is described, illustrated, and compared with a previous finding. This species was collected infecting Tropidacris collaris in the Yabotí biosphere reserve, Misiones province, Argentina. Until now, this entomopathogenic fungus has not been reported affecting T. collaris, and it is the first report for Argentina. From the cordyceps-like stromata of C. locustiphila it was possible to isolate for the first time the acremonium-like anamorph of this fungus. The identity of both, anamorph and teleomorph, was determined by morphological and molecular taxonomic studies, which challenges the recent new combination into Beauveria.Fil: Pelizza, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Scattolini, María Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Bardi, Cristian. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Lange, Carlos Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: Stenglein, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnolológico Mar del Plata. Instituto de Investigaciones en Biodiversidad y Biotecnología. Laboratorio de Biología Funcional y Biotecnología; ArgentinaFil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentin

Cave, Ronald D. - One of the best experts on this subject based on the ideXlab platform.

  • Efecto del Hongo Isaria fumosorosea Wize Sobre la Herbivoría por los Adultos del Escarabajo de Margen Amarillo, Microtheca ochroloma Stal (Coleoptera: Chrysomelidae)
    'Latin America Journals Online', 2017
    Co-Authors: Gámez Herrera Cecilia, Niño, Angie A., Avery, Pasco B., Cave, Ronald D.
    Abstract:

    The yellowmargined leaf beetle, Microtheca ochroloma Stål (Coleoptera: Chrysomelidae), is a pest that causes economic losses in the production of crucifers (Brassicaceae) in Argentina and the southeastern USA. Biological control using the entomopathogenic fungus Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) is an option for suppressing pest populations. This study evaluated the effect of this fungus on herbivory by adult beetles when they were fed leaves treated with I. fumosorosea. Four concentrations (0.1, 0.5, 1.0, and 2.0 g) of the formulation PFR-97 20% WDG Apopka strain 97 per 100 ml of distilled water and a distilled water only control were evaluated. Two trials each with 10 bok choy plants per treatment were performed, and each plant was infested with six adult beetles. On the seventh day, leaf area consumed by the beetles was measured. In the first trial, plants applied with 1.0 and 2.0 g/100 ml suffered significantly less damage than the control. In the second trial, there was a significant difference between the control and concentration of 2.0 g/100 ml. The control had 3.7 and 2.0% more damage compared to the other treatments in the first and second trials, respectively.Ceiba, 2016. Volumen 54(2):118-126El escarabajo de margen amarillo, Microtheca ochroloma Stål (Coleoptera: Chrysomelidae) es una plaga que causa pérdidas económicas en la producción de crucíferas (Brassicaceae) en Argentina y el sureste de los Estados Unidos. El control biológico utilizando el hongo entomopatógeno Isaria fumosorosea Wize (Hypocreales: Cordycipitaceae) es una alternativa para reducir las poblaciones de esta plaga. Este estudio evaluó el efecto que tiene este hongo en la cantidad de herbivoría por los adultos del escarabajo al ser alimentados con hojas aplicadas con I. fumosorosea. Se evaluaron cuatro concentraciones (0.1, 0.5, 1.0 y 2.0 g) de la formulación PFR-97 20% WDG cepa Apopka 97 por 100 ml de agua destilada y se usó como tratamiento control únicamente agua destilada. Se realizaron dos ensayos, cada ensayo con 10 plantas de bok choy por tratamiento, cada planta con seis escarabajos. A los 7 días se midió el área foliar consumida por los escarabajos. En el primer ensayo, las plantas aplicadas con 1.0 y 2.0 g/100 ml sufrieron significativamente menos daño que el control. Para el segundo ensayo hubo diferencia significativa entre el control y la concentración 2.0 g/100 ml. El control tuvo 3.7 y 2.0% más daño comparado con los demás tratamientos en el primer y segundo ensayo, respectivamente.Ceiba, 2016. Volumen 54(2):118-12

Zongqi Liang - One of the best experts on this subject based on the ideXlab platform.

  • taxonomic and phylogenetic characterizations reveal four new species of simplicillium Cordycipitaceae hypocreales from guizhou china
    Scientific Reports, 2021
    Co-Authors: Wanhao Chen, Yanfeng Han, Jiandong Liang, Zongqi Liang
    Abstract:

    Simplicillium species are commonly found from soil, seawater, rock surface, decayed wood, air and as symbiotic, endophytic, entomopathogenic and mycoparasitic fungi. Minority insect-associated species was reported. Simplicillium coccinellidae, S. hymenopterorum, S. neolepidopterorum and S. scarabaeoidea were introduced as the newly insect-associated species. The phylogenetic analyses of two combined datasets (LSU + RPB1 + TEF and SSU + ITS + LSU) revealed that S. coccinellidae and S. hymenopterorum were both nested in an independent clade. S. neolepidopterorum and S. scarabaeoidea have a close relationship with S. formicidae and S. lepidopterorum, respectively. S. neolepidopterorum can be easily distinguished from S. formicidae by ellipsoidal to cylindrical, solitary conidia which occasionally gather in short imbricate chains. S. scarabaeoidea could be easily distinguished from S. lepodopterorum by having longer phialides and larger conidia. Based on the morphological and phylogenetic conclusion, we determine the four newly generated isolates as new species of Simplicillium and a new combination is proposed in the genus Leptobacillium.

  • multi gene phylogenetic evidence indicates that pleurodesmospora belongs in Cordycipitaceae hypocreales hypocreomycetidae and pleurodesmospora lepidopterorum sp nov on pupa from china
    MycoKeys, 2021
    Co-Authors: Wanhao Chen, Yanfeng Han, Jiandong Liang, Weiyi Tian, Zongqi Liang
    Abstract:

    A new species, Pleurodesmospora lepidopterorum, isolated from a pupa, is introduced. Morphological comparisons and phylogenetic analyses based on multigene datasets (ITS+RPB1+RPB2+TEF) support the establishment of the new species. Pleurodesmospora lepidopterorum is distinguished from P. coccorum by its longer conidiogenous pegs located in the terminal or lateral conidiophores, and smaller subglobose or ellipsoidal conidia. A combined dataset of RPB1, RPB2, and TEF confirmed the taxonomic placement of Pleurodesmospora in Cordycipitaceae for the first time.

  • morphological and phylogenetic characterisations reveal three new species of samsoniella Cordycipitaceae hypocreales from guizhou china
    MycoKeys, 2020
    Co-Authors: Wanhao Chen, Yanfeng Han, Jiandong Liang, Weiyi Tian, Zongqi Liang
    Abstract:

    Samsoniella species have been found on lepidopteran larvae or pupae buried in soil or leaf litter. Three new species, Samsoniella hymenopterorum, S. coleopterorum and S. lepidopterorum, parasitic on hymenopteran larvae, coleopteran larvae and lepidopteran pupae, respectively, are reported. Morphological comparisons with extant species and DNA-based phylogenies from analysis of a multigene (ITS, RPB1, RPB2 and TEF) dataset supported the establishment of the new species. Unusually, all three new species have mononematous conidiophores. The new species are clearly distinct from other species in Samsoniella occurring in separate subclades.

  • Three novel insect-associated species of Simplicillium (Cordycipitaceae, Hypocreales) from Southwest China.
    MycoKeys, 2019
    Co-Authors: Wanhao Chen, Yanfeng Han, Jiandong Liang, Weiyi Tian, Chang Liu, Zongqi Liang
    Abstract:

    In this paper, we introduce three new species of Simplicillium, viz. S. cicadellidae, S. formicidae and S. lepidopterorum, which were isolated from an infected leafhopper, ant and carpenterworm, respectively. Morphological comparisons and phylogenetic analyses based on multigene datasets (LSU+RPB1+RPB2+TEF and ITS+LSU) support the establishment of the three new species. Simplicillium cicadellidae was distinguished from other species in morphological characteristics by having smaller phialides and ellipsoidal conidia, and lacking octahedral crystals. The reverse of colonies were yellowish (#FFBF00), especially in the middle, and radially sulcate. Simplicillium formicidae was morphologically distinguished from other by having longer phialides and filiform to fusoid conidia, and by lacking octahedral crystals. Simplicillium lepidopterorum was morphologically distinguished from other species by having smaller, ellipsoidal to fusiform conidia, and by lacking octahedral crystals. The reverse of the colony was pale white. The three new species are likely to be nourished by plant to animal (especially insect) nutrients based on the evolutionary pattern of the Hypocreales, and they are described herein as being clearly distinct from other species in Simplicillium.