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Maher Gtari - One of the best experts on this subject based on the ideXlab platform.
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frankia Coriariae sp nov an infective and effective microsymbiont isolated from Coriaria japonica
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Imen Nouioui, Faten Ghodhbanegtari, Manfred Rohde, Hanspeter Klenk, Maher GtariAbstract:The taxonomic description of a nitrogen-fixing actinobacterium, strain BMG5.1T, as a novel species within the genus Frankia was based on a polyphasic approach. The strain was isolated from the root nodules of Coriaria japonica, and it fulfilled Koch’s postulates by inducing effective nodules on Coriaria spp. and Datisca spp. Based on phenotypic and chemotaxonomic features, strain BMG5.1T is distinguishable from all other species of the genus Frankia . It is characterized by the presence of phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and glycophospholipids in its polar lipids; galactose, glucose, mannose and a trace of ribose as cellular sugars; meso-diaminopimelic acid as cell-wall peptidoglycan; C18 : 1ω9c as major fatty acid (>30 %); and MK-9(H6) (44.7 %) as predominant isoprenolog (>30 %). The 16S rRNA gene sequence similarities and the digital DNA–DNA hybridization values between the described novel strain and strains of the other species of the genus Frankia correspond to a range of 97–98.4 % and 22.1–24 %, respectively. The DNA G+C content is 70.2 mol%. On the basis of these results, strain BMG5.1T (=CECT 9032T=DSM 100624T) is proposed as the type strain of a novel species of the genus Frankia , named Frankia Coriariae sp. nov.
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Proteogenomics data for deciphering Frankia Coriariae interactions with root exudates from three host plants
Elsevier, 2017Co-Authors: Guylaine Miotello, Imen Nouioui, Amir Ktari, Abdellatif Gueddou, Faten Ghodhbane-gtari, Jean Armengaud, Maher GtariAbstract:Frankia Coriariae BMG5.1 cells were incubated with root exudates derived from compatible (Coriaria myrtifolia), incompatible (Alnus glutinosa) and non-actinorhizal (Cucumis melo) host plants. Bacteria cells and their exoproteomes were analyzed by high-throughput proteomics using a Q-Exactive HF high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. MS/MS spectra were assigned with two protein sequence databases derived from the closely-related genomes from strains BMG5.1 andDg1, the Frankia symbiont of Datisca glomerata. The tandem mass spectrometry data accompanying the manuscript describing the database searches and comparative analysis (Ktari et al., 2017, doi.org/10.3389/fmicb.2017.00720) [1] have been deposited to the ProteomeXchange with identifiers PXD005979 (whole cell proteomes) and PXD005980 (exoproteome data)
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Host Plant Compatibility Shapes the Proteogenome of Frankia Coriariae
Frontiers Media S.A., 2017Co-Authors: Maher Gtari, Imen Nouioui, Guylaine Miotello, Amir Ktari, Abdellatif Gueddou, Faten Ghodhbane-gtari, Indrani Sarkar, Arnab Sen, Jean ArmengaudAbstract:Molecular signaling networks in the actinorhizal rhizosphere select host-compatible Frankia strains, trigger the infection process and eventually the genesis of nitrogen-fixing nodules. The molecular triggers involved remain difficult to ascertain. Root exudates (RE) are highly dynamic substrates that play key roles in establishing the rhizosphere microbiome. RE are known to induce the secretion by rhizobia of Nod factors, polysaccharides, and other proteins in the case of legume symbiosis. Next-generation proteomic approach was here used to decipher the key bacterial signals matching the first-step recognition of host plant stimuli upon treatment of Frankia Coriariae strain BMG5.1 with RE derived from compatible (Coriaria myrtifolia), incompatible (Alnus glutinosa), and non-actinorhizal (Cucumis melo) host plants. The Frankia proteome dynamics were mainly driven by host compatibility. Both metabolism and signal transduction were the dominant activities for BMG5.1 under the different RE conditions tested. A second set of proteins that were solely induced by C. myrtifolia RE and were mainly linked to cell wall remodeling, signal transduction and host signal processing activities. These proteins may footprint early steps in receptive recognition of host stimuli before subsequent events of symbiotic recruitment
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Absence of Cospeciation between the Uncultured Frankia Microsymbionts and the Disjunct Actinorhizal Coriaria Species
BioMed research international, 2014Co-Authors: Imen Nouioui, Philippe Normand, Faten Ghodhbane-gtari, Maria P. Fernandez, Abdellatif Boudabous, Maher GtariAbstract:Coriaria is an actinorhizal plant that forms root nodules in symbiosis with nitrogen-fixing actinobacteria of the genus Frankia. This symbiotic association has drawn interest because of the disjunct geographical distribution of Coriaria in four separate areas of the world and in the context of evolutionary relationships between host plants and their uncultured microsymbionts. The evolution of Frankia-Coriaria symbioses was examined from a phylogenetic viewpoint using multiple genetic markers in both bacteria and host-plant partners. Total DNA extracted from root nodules collected from five species: C. myrtifolia, C. arborea, C. nepalensis, C. japonica, and C. microphylla, growing in the Mediterranean area (Morocco and France), New Zealand, Pakistan, Japan, and Mexico, respectively, was used to amplify glnA gene (glutamine synthetase), dnaA gene (chromosome replication initiator), and the nif DK IGS (intergenic spacer between nifD and nifK genes) in Frankia and the matK gene (chloroplast-encoded maturase K) and the intergenic transcribed spacers (18S rRNA-ITS1-5.8S rRNA-ITS2-28S rRNA) in Coriaria species. Phylogenetic reconstruction indicated that the radiations of Frankia strains and Coriaria species are not congruent. The lack of cospeciation between the two symbiotic partners may be explained by host shift at high taxonomic rank together with wind dispersal and/or survival in nonhost rhizosphere.
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First report on the occurrence of the uncultivated cluster 2 Frankia microsymbionts in soil outside the native actinorhizal host range area
Journal of Biosciences, 2013Co-Authors: Imen Nouioui, Philippe Normand, Faten Ghodhbane-gtari, Kawtar Fikri Benbrahim, Imed Sbissi, Maher GtariAbstract:The occurrence of uncultivated Frankia was evaluated in Tunisian soils by a plant-trapping assay using Coriaria myrtifolia seedlings. Despite the lack of this compatible host plant for more than two centuries, soil-borne Frankia cells were detected in one sampled soil as shown by the development of root nodules on 2-year-old seedlings. Based on gln A sequences, Tunisian trapped Frankia strains belong to the uncultivated cluster 2 strains that associate with other Coriaria species and also with Ceanothus , Datisca and Rosaceae actinorhizal species. This is the first report on the occurrence of Frankia cluster 2 strains in soils from areas lacking compatible host plant groups.
Imen Nouioui - One of the best experts on this subject based on the ideXlab platform.
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frankia Coriariae sp nov an infective and effective microsymbiont isolated from Coriaria japonica
International Journal of Systematic and Evolutionary Microbiology, 2017Co-Authors: Imen Nouioui, Faten Ghodhbanegtari, Manfred Rohde, Hanspeter Klenk, Maher GtariAbstract:The taxonomic description of a nitrogen-fixing actinobacterium, strain BMG5.1T, as a novel species within the genus Frankia was based on a polyphasic approach. The strain was isolated from the root nodules of Coriaria japonica, and it fulfilled Koch’s postulates by inducing effective nodules on Coriaria spp. and Datisca spp. Based on phenotypic and chemotaxonomic features, strain BMG5.1T is distinguishable from all other species of the genus Frankia . It is characterized by the presence of phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and glycophospholipids in its polar lipids; galactose, glucose, mannose and a trace of ribose as cellular sugars; meso-diaminopimelic acid as cell-wall peptidoglycan; C18 : 1ω9c as major fatty acid (>30 %); and MK-9(H6) (44.7 %) as predominant isoprenolog (>30 %). The 16S rRNA gene sequence similarities and the digital DNA–DNA hybridization values between the described novel strain and strains of the other species of the genus Frankia correspond to a range of 97–98.4 % and 22.1–24 %, respectively. The DNA G+C content is 70.2 mol%. On the basis of these results, strain BMG5.1T (=CECT 9032T=DSM 100624T) is proposed as the type strain of a novel species of the genus Frankia , named Frankia Coriariae sp. nov.
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Proteogenomics data for deciphering Frankia Coriariae interactions with root exudates from three host plants
Elsevier, 2017Co-Authors: Guylaine Miotello, Imen Nouioui, Amir Ktari, Abdellatif Gueddou, Faten Ghodhbane-gtari, Jean Armengaud, Maher GtariAbstract:Frankia Coriariae BMG5.1 cells were incubated with root exudates derived from compatible (Coriaria myrtifolia), incompatible (Alnus glutinosa) and non-actinorhizal (Cucumis melo) host plants. Bacteria cells and their exoproteomes were analyzed by high-throughput proteomics using a Q-Exactive HF high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. MS/MS spectra were assigned with two protein sequence databases derived from the closely-related genomes from strains BMG5.1 andDg1, the Frankia symbiont of Datisca glomerata. The tandem mass spectrometry data accompanying the manuscript describing the database searches and comparative analysis (Ktari et al., 2017, doi.org/10.3389/fmicb.2017.00720) [1] have been deposited to the ProteomeXchange with identifiers PXD005979 (whole cell proteomes) and PXD005980 (exoproteome data)
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Host Plant Compatibility Shapes the Proteogenome of Frankia Coriariae
Frontiers Media S.A., 2017Co-Authors: Maher Gtari, Imen Nouioui, Guylaine Miotello, Amir Ktari, Abdellatif Gueddou, Faten Ghodhbane-gtari, Indrani Sarkar, Arnab Sen, Jean ArmengaudAbstract:Molecular signaling networks in the actinorhizal rhizosphere select host-compatible Frankia strains, trigger the infection process and eventually the genesis of nitrogen-fixing nodules. The molecular triggers involved remain difficult to ascertain. Root exudates (RE) are highly dynamic substrates that play key roles in establishing the rhizosphere microbiome. RE are known to induce the secretion by rhizobia of Nod factors, polysaccharides, and other proteins in the case of legume symbiosis. Next-generation proteomic approach was here used to decipher the key bacterial signals matching the first-step recognition of host plant stimuli upon treatment of Frankia Coriariae strain BMG5.1 with RE derived from compatible (Coriaria myrtifolia), incompatible (Alnus glutinosa), and non-actinorhizal (Cucumis melo) host plants. The Frankia proteome dynamics were mainly driven by host compatibility. Both metabolism and signal transduction were the dominant activities for BMG5.1 under the different RE conditions tested. A second set of proteins that were solely induced by C. myrtifolia RE and were mainly linked to cell wall remodeling, signal transduction and host signal processing activities. These proteins may footprint early steps in receptive recognition of host stimuli before subsequent events of symbiotic recruitment
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Absence of Cospeciation between the Uncultured Frankia Microsymbionts and the Disjunct Actinorhizal Coriaria Species
BioMed research international, 2014Co-Authors: Imen Nouioui, Philippe Normand, Faten Ghodhbane-gtari, Maria P. Fernandez, Abdellatif Boudabous, Maher GtariAbstract:Coriaria is an actinorhizal plant that forms root nodules in symbiosis with nitrogen-fixing actinobacteria of the genus Frankia. This symbiotic association has drawn interest because of the disjunct geographical distribution of Coriaria in four separate areas of the world and in the context of evolutionary relationships between host plants and their uncultured microsymbionts. The evolution of Frankia-Coriaria symbioses was examined from a phylogenetic viewpoint using multiple genetic markers in both bacteria and host-plant partners. Total DNA extracted from root nodules collected from five species: C. myrtifolia, C. arborea, C. nepalensis, C. japonica, and C. microphylla, growing in the Mediterranean area (Morocco and France), New Zealand, Pakistan, Japan, and Mexico, respectively, was used to amplify glnA gene (glutamine synthetase), dnaA gene (chromosome replication initiator), and the nif DK IGS (intergenic spacer between nifD and nifK genes) in Frankia and the matK gene (chloroplast-encoded maturase K) and the intergenic transcribed spacers (18S rRNA-ITS1-5.8S rRNA-ITS2-28S rRNA) in Coriaria species. Phylogenetic reconstruction indicated that the radiations of Frankia strains and Coriaria species are not congruent. The lack of cospeciation between the two symbiotic partners may be explained by host shift at high taxonomic rank together with wind dispersal and/or survival in nonhost rhizosphere.
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First report on the occurrence of the uncultivated cluster 2 Frankia microsymbionts in soil outside the native actinorhizal host range area
Journal of Biosciences, 2013Co-Authors: Imen Nouioui, Philippe Normand, Faten Ghodhbane-gtari, Kawtar Fikri Benbrahim, Imed Sbissi, Maher GtariAbstract:The occurrence of uncultivated Frankia was evaluated in Tunisian soils by a plant-trapping assay using Coriaria myrtifolia seedlings. Despite the lack of this compatible host plant for more than two centuries, soil-borne Frankia cells were detected in one sampled soil as shown by the development of root nodules on 2-year-old seedlings. Based on gln A sequences, Tunisian trapped Frankia strains belong to the uncultivated cluster 2 strains that associate with other Coriaria species and also with Ceanothus , Datisca and Rosaceae actinorhizal species. This is the first report on the occurrence of Frankia cluster 2 strains in soils from areas lacking compatible host plant groups.
Hamdard Nagar - One of the best experts on this subject based on the ideXlab platform.
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Antihyperglycemic, antidyslipidemic and antioxidant activity of Rhus Coriaria in STZ-induced type 2 diabetic rats
African Journal of Pharmacy and Pharmacology, 2012Co-Authors: Tarique Anwer, Manju Sharma, K. K. Pillai, Gyas Khan, Mohammad M. Safhi, Hamdard NagarAbstract:The potential role of methanolic extract of Rhus Coriaria L. on hyperglycemia, dyslipidemia and lipid peroxidation (LPO) was studied in type 2 diabetic rats. Type 2 diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ, 100 mg/kg) to 2 days old rat pups. R. Coriaria (200 and 400 mg/kg) was administered orally once a day for 5 weeks after the animals were confirmed diabetic (that is, 90 days after STZ injection). A group of citrate control rats were also maintained which has received citrate buffer on the 2 nd day of their birth. Administration of R. Coriaria extract showed a significant (P < 0.001) decrease in blood glucose and tissue MDA levels as compared to type 2 diabetic control rats. The levels of total cholesterol (TCh), triglycerides (TG), low density lipoprotein cholesterol (LDL-C) and very low density lipoproteins cholesterol VLDL-C were also significantly (P < 0.001) decreased by R. Coriaria treatment when compared with type 2 diabetic control rats. In contrast the high density lipoprotein cholesterol (HDL-C) levels were significantly (P < 0.001) increased after treatment with R. Coriaria extract when compared with type 2 diabetic control rats. These results revealed that R. Coriaria possesses significant antihyperglycemic and antidyslipidemic activity along with potent antioxidant potential in type 2 diabetic mellitus.
Ehsan Ghaedi - One of the best experts on this subject based on the ideXlab platform.
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effects of hydroalcoholic extract of rhus Coriaria seed on glucose and insulin related biomarkers lipid profile and hepatic enzymes in nicotinamide streptozotocin induced type ii diabetic male mice
Research in Pharmaceutical Sciences, 2017Co-Authors: Akram Ahangarpour, Hamid Heidari, Majid Salehizade Junghani, Reza Absari, Mehdi Khoogar, Ehsan GhaediAbstract:Type 2 diabetes often leads to dislipidemia and abnormal activity of hepatic enzymes. The purpose of this study was to evaluate the antidiabetic and hypolipidemic properties of Rhus Coriaria (R. Coriaria) seed extrac on nicotinamide-streptozotocin induced type 2 diabetic mice. In this experimental study, 56 male Naval Medical Research Institute mice (30-35 g) were randomly separated into seven groups: control, diabetic group, diabetic mice treated with glibenclamide (0.25 mg/kg, as standard antidiabetic drug) or R. Coriaria seed extract in doses of 200 and 300 mg/kg, and control groups received these two doses of extract orally for 28 days. Induction of diabetes was done by intraperitoneal injection of nicotinamide and streptozotocin. Ultimately, body weight of mice, blood levels of glucose, insulin, hepatic enzymes, leptin, and lipid profile were assayed. After induction of type 2 diabetes, level of glucose, cholesterol, low density lipoprotein, serum glutamic oxaloacetic transaminase, and serum glutamic pyruvic transaminase increased and level of insulin and high density lipoprotein decreased remarkably. Administration of both doses of extract decreased level of glucose and cholesterol significantly in diabetic mice. LDL level decreased in treated group with dose of 300 mg/kg of the extract. Although usage of the extract improved level of other lipid profiles, insulin and hepatic enzymes, changes weren't significant. This study showed R. Coriaria seeds administration has a favorable effect in controlling some blood parameters in type 2 diabetes. Therefore it may be beneficial in the treatment of diabetes.
Eustoquio Martinezmolina - One of the best experts on this subject based on the ideXlab platform.
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micromonospora Coriariae sp nov isolated from root nodules of Coriaria myrtifolia
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Martha E Trujillo, Lorena Carro, Reiner M Kroppenstedt, Peter Schumann, Eustoquio MartinezmolinaAbstract:An actinomycete strain, NAR01T, was isolated from root nodules of a Coriaria plant. The 16S rRNA gene sequence of strain NAR01T showed most similarity to the type strains of Micromonospora endolithica (98.94 %) and Micromonospora chersina (98.4 %). The chemotaxonomic results obtained confirmed the taxonomic position of the isolate within the genus Micromonospora, and revealed differences at the species level. Physiological and biochemical tests showed that strain NAR01T could be clearly distinguished from its closest phylogenetic neighbours, while DNA–DNA hybridization results indicated that the isolate represents a novel species. On the basis of these results, strain NAR01T (=DSM 44875T=LMG 23557T) is proposed as the type strain of the novel species Micromonospora Coriariae sp. nov.