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Lucília Domingues - One of the best experts on this subject based on the ideXlab platform.
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Consolidated bioprocessing of Corn Cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts
Biotechnology for Biofuels, 2020Co-Authors: Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo, Lucília DominguesAbstract:Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which contains xylose as a major component in concentrations that can reach up to 40% of the total biomass in hardwoods and agricultural residues. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae—the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of Corn Cob-derived hemicellulose. These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of Corn Cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 g/g of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated Corn Cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.
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Consolidated bioprocessing of Corn Cob-derived hemicellulose: engineered industrial Saccharomyces cerevisiae as efficient whole cell biocatalysts
2020Co-Authors: Joana T. Cunha, Aloia Romaní, Kentaro Inokuma, Björn Johansson, Tomohisa Hasunuma, Akihiko Kondo, Lucília DominguesAbstract:Consolidated bioprocessing, which combines saccharolytic and fermentative abilities in a single microorganism, is receiving increased attention to decrease environmental and economic costs in lignocellulosic biorefineries. Nevertheless, the economic viability of lignocellulosic ethanol is also dependent of an efficient utilization of the hemicellulosic fraction, which is mainly composed of xylose and may comprise up to 40 % of the total biomass. This major bottleneck is mainly due to the necessity of chemical/enzymatic treatments to hydrolyze hemicellulose into fermentable sugars and to the fact that xylose is not readily consumed by Saccharomyces cerevisiae - the most used organism for large-scale ethanol production. In this work, industrial S. cerevisiae strains, presenting robust traits such as thermotolerance and improved resistance to inhibitors, were evaluated as hosts for the cell-surface display of hemicellulolytic enzymes and optimized xylose assimilation, aiming at the development of whole-cell biocatalysts for consolidated bioprocessing of Corn Cob-derived hemicellulose. These modifications allowed the direct production of ethanol from non-detoxified hemicellulosic liquor obtained by hydrothermal pretreatment of Corn Cob, reaching an ethanol titer of 11.1 g/L corresponding to a yield of 0.328 gram per gram of potential xylose and glucose, without the need for external hydrolytic catalysts. Also, consolidated bioprocessing of pretreated Corn Cob was found to be more efficient for hemicellulosic ethanol production than simultaneous saccharification and fermentation with addition of commercial hemicellulases. These results show the potential of industrial S. cerevisiae strains for the design of whole-cell biocatalysts and paves the way for the development of more efficient consolidated bioprocesses for lignocellulosic biomass valorization, further decreasing environmental and economic costs.
Thallada Bhaskar - One of the best experts on this subject based on the ideXlab platform.
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pyrolysis of agricultural biomass residues comparative study of Corn Cob wheat straw rice straw and rice husk
Bioresource Technology, 2017Co-Authors: Bijoy Biswas, Nidhi Pandey, Yashasvi Bisht, Rawel Singh, Jitendra Kumar, Thallada BhaskarAbstract:Abstract Pyrolysis studies on conventional biomass were carried out in fixed bed reactor at different temperatures 300, 350, 400 and 450 °C. Agricultural residues such as Corn Cob, wheat straw, rice straw and rice husk showed that the optimum temperatures for these residues are 450, 400, 400 and 450 °C respectively. The maximum bio-oil yield in case of Corn Cob, wheat straw, rice straw and rice husk are 47.3, 36.7, 28.4 and 38.1 wt% respectively. The effects of pyrolysis temperature and biomass type on the yield and composition of pyrolysis products were investigated. All bio-oils contents were mainly composed of oxygenated hydrocarbons. The higher area percentages of phenolic compounds were observed in the Corn Cob bio-oil than other bio-oils. From FT-IR and 1H NMR spectra showed a high percentage of aliphatic functional groups for all bio-oils and distribution of products is different due to differences in the composition of agricultural biomass.
Gerard Cornelissen - One of the best experts on this subject based on the ideXlab platform.
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the sorption and desorption of phosphate p ammonium n and nitrate n in cacao shell and Corn Cob biochars
Chemosphere, 2013Co-Authors: Sarah E Hale, Vanja Alling, Vegard Martinsen, Jan Mulder, Gijs D Breedveld, Gerard CornelissenAbstract:The sorption of PO4-P, NH4-N and NO3-N to cacao shell and Corn Cob biochars produced at 300-350°C was quantified. The biochars were used; (i) as received (unwashed), (ii) after rinsing with Millipore water and (iii) following leaching with Millipore water. In addition to sorption, desorption of PO4-P from the unwashed biochars was quantified. There was no sorption of PO4-P to either washed or rinsed biochars, but following leaching, both biochars adsorbed PO4-P and distribution coefficients (Kd L kg(-1)) were very similar for both materials (10(1.1±0.5) for cacao shell biochar and 10(1.0±0.2) for Corn Cob biochar). The BET surface area and micropore volume increased 80% and 60% for the cacao shell and Corn Cob biochars following leaching. After 60 d, 1483±45 mg kg(-1) and 172±1 mg kg(-1) PO4-P was released from the cacao shell and Corn Cob biochars. NH4-N was sorbed by both unwashed biochars, albeit weakly with Kd values around 10(2) L kg(-1). We speculate that NH4-N could bind via an electrostatic exchange with other cationic species on the surface of the biochar. There was no significant release or sorption of NO3-N from or to either of the biochars.
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the sorption and desorption of phosphate p ammonium n and nitrate n in cacao shell and Corn Cob biochars
Chemosphere, 2013Co-Authors: Sarah E Hale, Vanja Alling, Vegard Martinsen, Jan Mulder, Gijs D Breedveld, Gerard CornelissenAbstract:The sorption of PO4-P, NH4-N and NO3-N to cacao shell and Corn Cob biochars produced at 300-350 degrees C was quantified. The biochars were used; (i) as received (unwashed), (ii) after rinsing with ...
J.manuel Dominguez - One of the best experts on this subject based on the ideXlab platform.
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Extrusion and enzymatic hydrolysis as pretreatments on Corn Cob for biogas production
Renewable Energy, 2017Co-Authors: Noelia Pérez-rodríguez, Diana Garcia-bernet, J.manuel DominguezAbstract:Although agricultural wastes such as Corn Cob could be employed as substrates for biogas production, their lignocellulosic nature entails difficulties for their use. For this reason, a pretreatment of the biomass is recommended. Considering that there is limited information about the effects of extrusion as pretreatment on biogas production from biomass, this pretreatment alone or in combination with alkali and/or with the enzymatic hydrolysis was evaluated to improve the production of methane by anaerobic digestion of Corn Cob. The lignocellulosic composition, methane yields and biogas composition of the Corn Cob before and after pretreatments were compared. Among all the pretreatments studied, the sequential alkali extrusion and enzymatic hydrolysis (A+FE+H) pretreatment accomplished the major improvement with an increase of the methane volume produced of 22.3% in regards to the anaerobic digestion of the raw Corn Cob.
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Effects of enzymatic hydrolysis and ultrasounds pretreatments on Corn Cob and vine trimming shoots for biogas production
Bioresource Technology, 2016Co-Authors: Noelia Pérez-rodríguez, Diana Garcia-bernet, J.manuel DominguezAbstract:Due to their lignocellulosic nature, Corn Cob and vine trimming shoots (VTS) could be valorized by anaerobic digestion for biogas production. To enhance the digestibility of substrates, pretreatments of lignocellulosic materials are recommended. The effect of enzymatic hydrolysis, ultrasounds pretreatments (US) and the combination of both was assayed in lignocellulosic composition, methane, and biogas yields. The pretreatments leaded to a reduction in lignin and an increase in neutral detergent soluble compounds making Corn Cob and VTS more amendable for biogas conversion. The US were negative for biogas production from both substrates and in particular strongly detrimental for VTS. On the opposite side, the enzymatic hydrolysis was certainly beneficial increasing 59.8% and 14.6% the methane production from VTS and Corn Cob, respectively. The prior application of US did not potentiate (or not sufficiently) the improvement in the methane production reflected by the enzymatic hydrolysis pretreatment of VTS and Corn Cob.
Roger Ruan - One of the best experts on this subject based on the ideXlab platform.
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microwave assisted catalytic pyrolysis of torrefied Corn Cob for phenol rich bio oil production over fe modified bio char catalyst
Journal of Analytical and Applied Pyrolysis, 2019Co-Authors: Leilei Dai, Roger Ruan, Yunpu Wang, Yuhuan Liu, Lin Jiang, Zihong Zeng, Xiaojie TianAbstract:Abstract Microwave-assisted catalytic pyrolysis of torrefied Corn Cob into phenol-rich bio-oil on Fe modified bio-char catalyst was investigated in this study. The well-developed surface pore was confirmed by scanning electronic microscope (SEM) images and nitrogen adsorption/desorption isotherms, indicating that the porous structure of bio-char depended on the Fe modification to a large extent. Temperature-programmed desorption of NH3 (NH3-TPD) analysis showed that the Fe modified bio-char catalyst mainly presented strong acid sites. The use of bio-char catalyst can decrease the bio-oil yield and increase the gas yield but the biochar did not experience any significant change because of the ex-situ catalysis mode. Catalytic pyrolysis of torrefied Corn Cob using Fe modified bio-char catalyst was able to produce the higher yields and selectivities of phenol and cresol, where the catalytic performance of the bio-char catalyst was superior to commercial activated carbon. In addition, the catalyst deactivation and regenation tests were also conducted to evaluate the service life of the catalyst.
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catalytic fast pyrolysis of torrefied Corn Cob to aromatic hydrocarbons over ni modified hierarchical zsm 5 catalyst
Bioresource Technology, 2019Co-Authors: Leilei Dai, Roger Ruan, Yunpu Wang, Yuhuan Liu, Dengle Duan, Yunfeng Zhao, Lin JiangAbstract:Abstract Catalytic fast pyrolysis (CFP) of torrefied Corn Cob using Ni-modified hierarchical ZSM-5 catalyst was conducted in this study. The prepared catalysts were characterized by N2 adsorption and desorption (N2-BET), X-ray diffraction (XRD), and temperature-programmed desorption of NH3 (NH3-TPD). NaOH solution treatment resulted in the lower peak intensities of hierarchical ZSM-5 catalyst in the XRD patterns while Ni modification improved the catalyst framework. In addition, NaOH solution treatment created some mesopores or macropores, but the incorporation of Ni reduced BET surface area and volume of micropores. Though the addition of Ni lowered the acidity of catalyst, Ni-modified hierarchical ZSM-5 catalyst led to higher yields and of aromatic hydrocarbons. What is more, hierarchical ZSM-5 catalysts significantly improved the selectivities of mono-aromatics. Kinetic analysis shows that CFP of torrefied Corn Cob was second-order reaction and the addition of Ni can obtain a lower activation energy compared with hierarchical ZSM-5 catalyst.
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microwave pyrolysis of Corn Cob and characteristics of the pyrolytic chars
Energy Sources Part A-recovery Utilization and Environmental Effects, 2010Co-Authors: Phillip Steele, Roger RuanAbstract:Abstract Microwave pyrolysis is a new process for converting biomass to bio-oil, syngas, and solid char. In this study, pyrolysis of Corn Cob was performed in an inert environment at atmospheric pressure and temperatures ranging from 300 to 600°C. The aim of this work was to study the effect of pyrolysis conditions on the characteristics of the solid char residue. The char was characterized using scanning electron microscopy, Fourier transform infrared spectroscopy, elemental analyzer, BET surface area analysis, and inductive coupled plasma. The char yield from pyrolysis decreased significantly to 23% with an increase in temperature to 600°C. SEM analysis indicated that pyrolysis of Corn Cob led to a stepwise accumulation of inorganic matter onto the exposed surface, and some organic matter melted, resulting in the formation of hollow cavities by the evolving volatiles. Fourier transform infrared spectroscopy results showed a continuous decrease in the intensity of the hydroxyl group stretch with temperat...