The Experts below are selected from a list of 3150 Experts worldwide ranked by ideXlab platform
Paul A Yates - One of the best experts on this subject based on the ideXlab platform.
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myh11 lineage Corneal endothelial cells and ascs populate Corneal Endothelium
Investigative Ophthalmology & Visual Science, 2019Co-Authors: Bruce A. Corliss, Corbin Mathews, Richard Doty, Chris M. Smolko, Hamzah Shariff, Shayn M. Peirce, Kathleen Fitzgerald, Paul A YatesAbstract:Purpose: To establish Myh11 as a marker of a subset of Corneal endothelial cells (CECs), and to demonstrate the feasibility of restoring the Corneal Endothelium with Myh11-lineage (Myh11-Lin[+]) adipose-derived stromal cells (ASCs). Methods: Intraperitoneal administration of tamoxifen and (Z)-4-hydroxytamoxifen eyedrops were used to trace the lineage of Myh11-expressing cells with the Myh11-Cre-ERT2-flox-tdTomato mouse model. Immunostaining and Western blot characterized marker expression and spatial distribution of Myh11-Lin(+) cells in the Cornea, and administration of 5-ethynyl-2'-deoxyuridine labeled proliferating cells. ASCs were isolated from epididymal adipose Myh11+ mural cells and treated with Cornea differentiation media to evaluate Corneal endothelial differentiation potential. Differentiated ASCs were injected into the anterior chamber to test for incorporation into Corneal Endothelium following scratch injury. Results: A subset of CECs express Myh11, a marker previously thought restricted to only mural cells. Myh11-Lin(+) CECs marked a stable subpopulation of cells in the Cornea Endothelium. Myh11-Lin(+) ASCs undergo CEC differentiation in vitro and incorporate into injured Corneal Endothelium. Conclusions: Dystrophy and dysfunction of the Corneal Endothelium accounts for almost half of all Corneal transplants, the maintenance of the Cornea Endothelium is poorly understood, and there are a lack of mouse models to study specific CEC populations. We establish a mouse model that can trace the cell fate of a subpopulation of CECs based on Myh11 expression. A subset of ASCs that share this Myh11 transcriptional lineage are capable of differentiating into CECs that can incorporate into injured Corneal Endothelium, revealing a potential cell source for creating engineered transplant material.
Jean-charles Pinoli - One of the best experts on this subject based on the ideXlab platform.
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Comparison and supervised learning of segmentation methods dedicated to specular microscope images of Corneal Endothelium
International Journal of Biomedical Imaging, 2014Co-Authors: Yann Gavet, Jean-charles PinoliAbstract:The Cornea is the front of the eye. Its inner cell layer, called the Endothelium, is important because it is closely related to the light transparency of the Cornea. An in vivo observation of this layer is performed by using specular microscopy to evaluate the health of the cells: a high spatial density will result in a good transparency. Thus, the main criterion required by ophthalmologists is the cell density of the Cornea Endothelium, mainly obtained by an image segmentation process. Different methods can perform the image segmentation of these cells, and the three most performing methods are studied here. The question for the ophthalmologists is how to choose the best algorithm and to obtain the best possible results with it. This paper presents a methodology to compare these algorithms together. Moreover, by the way of geometric dissimilarity criteria, the algorithms are tuned up, and the best parameter values are thus proposed to the expert ophthalmologists.
Bruce A. Corliss - One of the best experts on this subject based on the ideXlab platform.
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myh11 lineage Corneal endothelial cells and ascs populate Corneal Endothelium
Investigative Ophthalmology & Visual Science, 2019Co-Authors: Bruce A. Corliss, Corbin Mathews, Richard Doty, Chris M. Smolko, Hamzah Shariff, Shayn M. Peirce, Kathleen Fitzgerald, Paul A YatesAbstract:Purpose: To establish Myh11 as a marker of a subset of Corneal endothelial cells (CECs), and to demonstrate the feasibility of restoring the Corneal Endothelium with Myh11-lineage (Myh11-Lin[+]) adipose-derived stromal cells (ASCs). Methods: Intraperitoneal administration of tamoxifen and (Z)-4-hydroxytamoxifen eyedrops were used to trace the lineage of Myh11-expressing cells with the Myh11-Cre-ERT2-flox-tdTomato mouse model. Immunostaining and Western blot characterized marker expression and spatial distribution of Myh11-Lin(+) cells in the Cornea, and administration of 5-ethynyl-2'-deoxyuridine labeled proliferating cells. ASCs were isolated from epididymal adipose Myh11+ mural cells and treated with Cornea differentiation media to evaluate Corneal endothelial differentiation potential. Differentiated ASCs were injected into the anterior chamber to test for incorporation into Corneal Endothelium following scratch injury. Results: A subset of CECs express Myh11, a marker previously thought restricted to only mural cells. Myh11-Lin(+) CECs marked a stable subpopulation of cells in the Cornea Endothelium. Myh11-Lin(+) ASCs undergo CEC differentiation in vitro and incorporate into injured Corneal Endothelium. Conclusions: Dystrophy and dysfunction of the Corneal Endothelium accounts for almost half of all Corneal transplants, the maintenance of the Cornea Endothelium is poorly understood, and there are a lack of mouse models to study specific CEC populations. We establish a mouse model that can trace the cell fate of a subpopulation of CECs based on Myh11 expression. A subset of ASCs that share this Myh11 transcriptional lineage are capable of differentiating into CECs that can incorporate into injured Corneal Endothelium, revealing a potential cell source for creating engineered transplant material.
Yann Gavet - One of the best experts on this subject based on the ideXlab platform.
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Comparison and supervised learning of segmentation methods dedicated to specular microscope images of Corneal Endothelium
International Journal of Biomedical Imaging, 2014Co-Authors: Yann Gavet, Jean-charles PinoliAbstract:The Cornea is the front of the eye. Its inner cell layer, called the Endothelium, is important because it is closely related to the light transparency of the Cornea. An in vivo observation of this layer is performed by using specular microscopy to evaluate the health of the cells: a high spatial density will result in a good transparency. Thus, the main criterion required by ophthalmologists is the cell density of the Cornea Endothelium, mainly obtained by an image segmentation process. Different methods can perform the image segmentation of these cells, and the three most performing methods are studied here. The question for the ophthalmologists is how to choose the best algorithm and to obtain the best possible results with it. This paper presents a methodology to compare these algorithms together. Moreover, by the way of geometric dissimilarity criteria, the algorithms are tuned up, and the best parameter values are thus proposed to the expert ophthalmologists.
Shayn M. Peirce - One of the best experts on this subject based on the ideXlab platform.
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myh11 lineage Corneal endothelial cells and ascs populate Corneal Endothelium
Investigative Ophthalmology & Visual Science, 2019Co-Authors: Bruce A. Corliss, Corbin Mathews, Richard Doty, Chris M. Smolko, Hamzah Shariff, Shayn M. Peirce, Kathleen Fitzgerald, Paul A YatesAbstract:Purpose: To establish Myh11 as a marker of a subset of Corneal endothelial cells (CECs), and to demonstrate the feasibility of restoring the Corneal Endothelium with Myh11-lineage (Myh11-Lin[+]) adipose-derived stromal cells (ASCs). Methods: Intraperitoneal administration of tamoxifen and (Z)-4-hydroxytamoxifen eyedrops were used to trace the lineage of Myh11-expressing cells with the Myh11-Cre-ERT2-flox-tdTomato mouse model. Immunostaining and Western blot characterized marker expression and spatial distribution of Myh11-Lin(+) cells in the Cornea, and administration of 5-ethynyl-2'-deoxyuridine labeled proliferating cells. ASCs were isolated from epididymal adipose Myh11+ mural cells and treated with Cornea differentiation media to evaluate Corneal endothelial differentiation potential. Differentiated ASCs were injected into the anterior chamber to test for incorporation into Corneal Endothelium following scratch injury. Results: A subset of CECs express Myh11, a marker previously thought restricted to only mural cells. Myh11-Lin(+) CECs marked a stable subpopulation of cells in the Cornea Endothelium. Myh11-Lin(+) ASCs undergo CEC differentiation in vitro and incorporate into injured Corneal Endothelium. Conclusions: Dystrophy and dysfunction of the Corneal Endothelium accounts for almost half of all Corneal transplants, the maintenance of the Cornea Endothelium is poorly understood, and there are a lack of mouse models to study specific CEC populations. We establish a mouse model that can trace the cell fate of a subpopulation of CECs based on Myh11 expression. A subset of ASCs that share this Myh11 transcriptional lineage are capable of differentiating into CECs that can incorporate into injured Corneal Endothelium, revealing a potential cell source for creating engineered transplant material.