The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Tsuyoshi Kimura - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Shuji Sasaki, Shinya Hattori, Takako Honda, Yoshihide Hashimoto, Seiichi Funamoto, Tsuyoshi KimuraAbstract:Abstract To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 °C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Yoshihide Hashimoto, Shuji Sasaki, Shinya Hattori, Takako Honda, Seiichi Funamoto, Tsuyoshi Kimura, Kwangwoo NamAbstract:To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 degrees C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
Yoshihide Hashimoto - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Shuji Sasaki, Shinya Hattori, Takako Honda, Yoshihide Hashimoto, Seiichi Funamoto, Tsuyoshi KimuraAbstract:Abstract To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 °C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Yoshihide Hashimoto, Shuji Sasaki, Shinya Hattori, Takako Honda, Seiichi Funamoto, Tsuyoshi Kimura, Kwangwoo NamAbstract:To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 degrees C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
Seiichi Funamoto - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Shuji Sasaki, Shinya Hattori, Takako Honda, Yoshihide Hashimoto, Seiichi Funamoto, Tsuyoshi KimuraAbstract:Abstract To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 °C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Yoshihide Hashimoto, Shuji Sasaki, Shinya Hattori, Takako Honda, Seiichi Funamoto, Tsuyoshi Kimura, Kwangwoo NamAbstract:To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 degrees C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
Shuji Sasaki - One of the best experts on this subject based on the ideXlab platform.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Shuji Sasaki, Shinya Hattori, Takako Honda, Yoshihide Hashimoto, Seiichi Funamoto, Tsuyoshi KimuraAbstract:Abstract To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 °C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
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preparation and characterization of decellularized Cornea using high hydrostatic pressurization for Corneal tissue engineering
Biomaterials, 2010Co-Authors: Yoshihide Hashimoto, Shuji Sasaki, Shinya Hattori, Takako Honda, Seiichi Funamoto, Tsuyoshi Kimura, Kwangwoo NamAbstract:To prepare acellular Corneal scaffold, we used high-hydrostatic pressurization (HHP) to decellularize porcine Cornea. The HHP method disrupts cells by hydrostatic pressurization, and then the disrupted cells' components are removed by washing with a cell culture medium. Porcine Corneas were hydrostatically pressed at 980 MPa at 10 or 30 degrees C for 10 min to make them opaque. There was no change in the thickness of the Corneas immediately after the pressurization, but they swelled during the washing process. The Cornea swelling caused by HHP was suppressed when medium containing 3.5% w/v dextran was used. For H-E staining of the Cornea decellularized with the HHP method, the complete removal of Corneal cells was confirmed. Furthermore, when the Corneas were immersed in glycerol for 1 hour, their optical properties were restored to those of native Corneas. In an animal study, when acellular porcine Corneas were implanted into rabbit Cornea, no immune reaction occurred and the turbid Corneas became clear. The decellularized Corneas obtained through HHP could be useful as a Corneal scaffold for tissue regeneration.
Thibaud Garcin - One of the best experts on this subject based on the ideXlab platform.
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ex vivo model of herpes simplex virus type i dendritic and geographic keratitis using a Corneal active storage machine
PLOS ONE, 2020Co-Authors: Emilie Courrier, Corantin Maurin, Victor Lambert, Didier Renault, Thomas Bourlet, Sylvie Pillet, Paul O Verhoeven, Fabien Forest, Chantal Perrache, Thibaud GarcinAbstract:Background Herpetic keratitis (HK) models using whole human Corneas are essential for studying virus-host relationships, because of high species specificity and the role of interactions between Corneal cell populations that cell culture cannot reproduce. Nevertheless, the two current Corneal storage methods (hypothermia and organ culture (OC)) do not preserve Corneas in good physiological condition, as they are characterized by epithelial abrasion, stromal oedema, and excessive endothelial mortality. Methods To rehabilitate human Corneas intended for scientific use, we used an active storage machine (ASM) that restores two physiological parameters that are essential for Corneal homeostasis: intraocular pressure and storage medium renewal (21mmHg and 2.6 μL/min, respectively). ASM storage regenerates a normal multilayer epithelium in 2 weeks. We infected six pairs of Corneas unsuitable for graft by inoculating the epithelium with herpes simplex virus type 1 (HSV-1), and compared each ASM-stored Cornea with the other Cornea stored in the same medium using the conventional OC method. Results Only Corneas in the ASM developed a dendritic (n = 3) or geographic (n = 2) epithelial ulcer reproducing typical HSV-1-induced clinical lesions. Corneas in OC showed only extensive desquamations. None of the uninfected controls showed epithelial damage. Histology, immunohistochemistry, transmission electron microscopy and polymerase chain reaction on Corneal tissue confirmed infection in all cases (excluding negative controls). Conclusions The ASM provides an innovative ex vivo model of HK in whole human Cornea that reproduces typical epithelial lesions.