The Experts below are selected from a list of 228 Experts worldwide ranked by ideXlab platform
Androulla Efstratiou - One of the best experts on this subject based on the ideXlab platform.
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toxigenic Corynebacterium ulcerans in human and non toxigenic Corynebacterium diphtheriae in cat
new microbes and new infections, 2013Co-Authors: Liselot Detemmerman, Androulla Efstratiou, C Schirvel, Kristof Emmerechts, Ingrid Wybo, Oriane Soetens, Dimitri Rousseaux, Denis PierardAbstract:Corynebacterium diphtheriae and Corynebacterium ulcerans are rarely isolated from clinical samples in Belgium. A case of toxigenic C. ulcerans in a woman is described, which confirms that this pathogen is still present. During investigation of the patient's cats, only a non-toxigenic toxin-bearing C. diphtheriae strain was detected.
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Toxigenic Corynebacterium ulcerans in human and non‐toxigenic Corynebacterium diphtheriae in cat
new microbes and new infections, 2013Co-Authors: Liselot Detemmerman, Androulla Efstratiou, C Schirvel, Kristof Emmerechts, Ingrid Wybo, Oriane Soetens, Dimitri Rousseaux, Denis PierardAbstract:Corynebacterium diphtheriae and Corynebacterium ulcerans are rarely isolated from clinical samples in Belgium. A case of toxigenic C. ulcerans in a woman is described, which confirms that this pathogen is still present. During investigation of the patient's cats, only a non-toxigenic toxin-bearing C. diphtheriae strain was detected.
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International nomenclature for Corynebacterium diphtheriae ribotypes.
Research in Microbiology, 2004Co-Authors: Patrick A. D. Grimont, Androulla Efstratiou, Francine Grimont, Aruni De Zoysa, Izabella K. Mazurova, Corinne Ruckly, Monique Lejay-collin, Sylvie Martin-delautre, Béatrice RegnaultAbstract:The nomenclature of Corynebacterium diphtheriae ribotypes is presented. A total of 86 ribotypes obtained after BstEII digestion were given a geographic name chosen to reflect the place where one of the strains was isolated or studied.
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nontoxigenic Corynebacterium diphtheriae an emerging pathogen in england and wales
Emerging Infectious Diseases, 2000Co-Authors: Mark Reacher, Androulla Efstratiou, Mary Ramsay, J M White, A De Zoysa, Ginder Mann, A Mackay, R C GeorgeAbstract:Confirmed isolates of nontoxigenic Corynebacterium diphtheriae in England and Wales increased substantially from 1986 to 1994. Ribotyping of 121 isolates confirmed in 1995 showed that 90 were of a single strain isolated exclusively from the throat; none had previously been identified in toxigenic strains from U.K. or non-U.K. residents. The upward trend in nontoxigenic C. diphtheriae probably represented increased ascertainment, although dissemination of a particular strain or clone may have been a factor.
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Use of Amplified Fragment Length Polymorphisms for Typing Corynebacterium diphtheriae
Journal of clinical microbiology, 2000Co-Authors: Aruni De Zoysa, Androulla EfstratiouAbstract:Amplified fragment length polymorphism (AFLP) was investigated for the differentiation of Corynebacterium diphtheriae isolates. Analysis using Taxotron revealed 10 distinct AFLP profiles among 57 isolates. Strains with ribotype patterns D1, D4, and D12 could not be distinguished; however, the technique discriminated isolates of ribotype patterns D3, D6, and D7 further. AFLP was rapid, fairly inexpensive, and reproducible and could be used as an alternative to ribotyping.
Denis Pierard - One of the best experts on this subject based on the ideXlab platform.
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toxigenic Corynebacterium ulcerans in human and non toxigenic Corynebacterium diphtheriae in cat
new microbes and new infections, 2013Co-Authors: Liselot Detemmerman, Androulla Efstratiou, C Schirvel, Kristof Emmerechts, Ingrid Wybo, Oriane Soetens, Dimitri Rousseaux, Denis PierardAbstract:Corynebacterium diphtheriae and Corynebacterium ulcerans are rarely isolated from clinical samples in Belgium. A case of toxigenic C. ulcerans in a woman is described, which confirms that this pathogen is still present. During investigation of the patient's cats, only a non-toxigenic toxin-bearing C. diphtheriae strain was detected.
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Toxigenic Corynebacterium ulcerans in human and non‐toxigenic Corynebacterium diphtheriae in cat
new microbes and new infections, 2013Co-Authors: Liselot Detemmerman, Androulla Efstratiou, C Schirvel, Kristof Emmerechts, Ingrid Wybo, Oriane Soetens, Dimitri Rousseaux, Denis PierardAbstract:Corynebacterium diphtheriae and Corynebacterium ulcerans are rarely isolated from clinical samples in Belgium. A case of toxigenic C. ulcerans in a woman is described, which confirms that this pathogen is still present. During investigation of the patient's cats, only a non-toxigenic toxin-bearing C. diphtheriae strain was detected.
Hiroshi Nishimasu - One of the best experts on this subject based on the ideXlab platform.
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structural basis for the promiscuous pam recognition by Corynebacterium diphtheriae cas9
Nature Communications, 2019Co-Authors: Seiichi Hirano, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Omar O Abudayyeh, Jonathan S Gootenberg, Hiroshi NishimasuAbstract:The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets bearing a protospacer adjacent motif (PAM) and complementarity to an RNA guide. Unlike other Cas9 orthologs, Corynebacterium diphtheriae Cas9 (CdCas9) recognizes the promiscuous NNRHHHY PAM. However, the CdCas9-mediated PAM recognition mechanism remains unknown. Here, we report the crystal structure of CdCas9 in complex with the guide RNA and its target DNA at 2.9 A resolution. The structure reveals that CdCas9 recognizes the NNRHHHY PAM via a combination of van der Waals interactions and base-specific hydrogen bonds. Moreover, we find that CdCas9 exhibits robust DNA cleavage activity with the optimal 22-nucleotide length guide RNAs. Our findings highlight the mechanistic diversity of the PAM recognition by Cas9 orthologs, and provide a basis for the further engineering of the CRISPR-Cas9 genome-editor nucleases. The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 A crystal structure of CdCas9 in complex with the guide RNA and its target DNA, which is of interest for engineering of CRISPR-Cas9 genome-editor nucleases.
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Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
Nature Publishing Group, 2019Co-Authors: Seiichi Hirano, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Omar O Abudayyeh, Jonathan S Gootenberg, Hiroshi Nishimasu, Osamu NurekiAbstract:The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 Å crystal structure of CdCas9 in complex with the guide RNA and its target DNA, which is of interest for engineering of CRISPR-Cas9 genome-editor nucleases
Aleksandra A Zasada - One of the best experts on this subject based on the ideXlab platform.
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Corynebacterium diphtheriae infections currently and in the past.
Przeglad epidemiologiczny, 2015Co-Authors: Aleksandra A ZasadaAbstract:Along with the introduction of common obligatory vaccinations against diphtheria, the disease has been limited in developed countries. However, diphtheria is still endemic in developing countries. Due to a growing popularity of visiting these countries, there is a risk of importation of the disease to Europe. Studies revealed that over 60% of persons aged >40 years in the Polish population do not have a protective level of antibodies against diphtheria. Furthermore, an access to diphtheria antitoxin, which is essential in diphtheria treatment, is now hardly accessible in Europe. On the other hand, in many countries, including Poland, new infections caused by non-toxigenic Corynebacterium diphtheriae have been emerged. Such infections are frequently manifested by bacteraemia and endocarditis with a high fatality rate, amounting even to 41%.
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The utility of the PCR melting profile technique for typing Corynebacterium diphtheriae isolates
Letters in applied microbiology, 2014Co-Authors: Aleksandra A Zasada, Edgar Badell, Kamila Formińska, Tomasz Wołkowicz, Nicole GuisoAbstract:UNLABELLED Selection of appropriate typing method depends on a number of factors, including the scale of the investigation, the rapidity required of the results and the financial and technical resources available. Several typing methods have been applied to Corynebacterium diphtheriae genotyping, but most are laborious and time-consuming or require expensive equipment. We report an evaluation of the utility of the PCR melting profile technique for simple and easy-to-perform genotyping of C. diphtheriae. We compared the method with ribotyping-the 'gold standard' for C. diphtheriae typing-and PFGE, MLST, AFLP, RAPD and spoligotyping. SIGNIFICANCE AND IMPACT OF THE STUDY Occurrence of Corynebacterium diphtheriae infections-in the form of diphtheria in endemic countries and in the form of invasive infections in countries with high antidiphtheria vaccination coverage-indicates the need for maintenance of ability to genotype this pathogen by laboratories. Application of an appropriate typing method is essential not only in outbreak investigations for understanding and predicting epidemics but also in monitoring of the evolution and spread of epidemic clones of C. diphtheriae. The PCR melting profile method presented in the study is a good alternative for C. diphtheriae typing.
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Nontoxigenic highly pathogenic clone of Corynebacterium diphtheriae, Poland, 2004-2012.
Emerging infectious diseases, 2013Co-Authors: Aleksandra A ZasadaAbstract:Twenty-five cases of nontoxigenic Corynebacterium diphtheriae infection were recorded in Poland during 2004–2012, of which 18 were invasive. Alcoholism, homelessness, hepatic cirrhosis, and dental caries were predisposing factors for infection. However, for 17% of cases, no concomitant diseases or predisposing factors were found.
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Characterization and Comparison of Invasive Corynebacterium diphtheriae Isolates from France and Poland
Journal of clinical microbiology, 2011Co-Authors: E. Farfour, Edgar Badell, Aleksandra A Zasada, Helmut Hotzel, Herbert Tomaso, Sophie Guillot, Nicole GuisoAbstract:Corynebacterium diphtheriae, the agent of diphtheria, is rarely responsible for bacteremia. However, high numbers of bacteremia have been reported in countries with extensive immunization coverage. Here, we used molecular and phenotypic tools to characterize and compare 42 invasive isolates collected in France (including New Caledonia) and Poland over a 23-year period.
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Types of Corynebacterium diphtheriae strains isolated in Poland in 2004-2008
Medycyna doswiadczalna i mikrobiologia, 2008Co-Authors: Aleksandra A Zasada, Milena Baczewska-rejAbstract:Biotyping and genotyping were performed for Corynebacterium diphtheriae strains isolated from humans in Poland during last several years. All the strains belonged to the same biotype and genetic clone. Similar situation has not yet been described in international literature, although nontoxigenic C. diphtheriae strains have been isolated more frequently in developed countries. However, strains isolated and investigated in other countries revealed heterogeneity of biotypes and genotypes.
Omar O Abudayyeh - One of the best experts on this subject based on the ideXlab platform.
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structural basis for the promiscuous pam recognition by Corynebacterium diphtheriae cas9
Nature Communications, 2019Co-Authors: Seiichi Hirano, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Omar O Abudayyeh, Jonathan S Gootenberg, Hiroshi NishimasuAbstract:The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets bearing a protospacer adjacent motif (PAM) and complementarity to an RNA guide. Unlike other Cas9 orthologs, Corynebacterium diphtheriae Cas9 (CdCas9) recognizes the promiscuous NNRHHHY PAM. However, the CdCas9-mediated PAM recognition mechanism remains unknown. Here, we report the crystal structure of CdCas9 in complex with the guide RNA and its target DNA at 2.9 A resolution. The structure reveals that CdCas9 recognizes the NNRHHHY PAM via a combination of van der Waals interactions and base-specific hydrogen bonds. Moreover, we find that CdCas9 exhibits robust DNA cleavage activity with the optimal 22-nucleotide length guide RNAs. Our findings highlight the mechanistic diversity of the PAM recognition by Cas9 orthologs, and provide a basis for the further engineering of the CRISPR-Cas9 genome-editor nucleases. The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 A crystal structure of CdCas9 in complex with the guide RNA and its target DNA, which is of interest for engineering of CRISPR-Cas9 genome-editor nucleases.
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Structural basis for the promiscuous PAM recognition by Corynebacterium diphtheriae Cas9
Nature Publishing Group, 2019Co-Authors: Seiichi Hirano, Takuro Horii, Ryuichiro Ishitani, Izuho Hatada, Feng Zhang, Omar O Abudayyeh, Jonathan S Gootenberg, Hiroshi Nishimasu, Osamu NurekiAbstract:The RNA-guided DNA endonuclease Cas9 from Corynebacterium diphtheriae (CdCas9) recognizes a promiscuous protospacer adjacent motif (PAM). Here the authors provide insights into the CdCas9-mediated PAM recognition mechanism by determining the 2.9 Å crystal structure of CdCas9 in complex with the guide RNA and its target DNA, which is of interest for engineering of CRISPR-Cas9 genome-editor nucleases
