The Experts below are selected from a list of 15939 Experts worldwide ranked by ideXlab platform
Alexander J. Krynitsky - One of the best experts on this subject based on the ideXlab platform.
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rapid determination of para phenylenediamine by gas chromatography mass spectrometry with selected ion monitoring in henna containing Cosmetic Products
Journal of Chromatography A, 2011Co-Authors: Perry G Wang, Alexander J. KrynitskyAbstract:A rapid method for the determination of para-phenylenediamine (PPD) in Cosmetic Products, such as henna tattoos has been developed and evaluated. This analytical procedure involved extracting a 10mg test portion of Cosmetic Product in 10mL of ethyl acetate, followed by determination by gas chromatography–mass spectrometry in the selected ion monitoring mode (GC/MS-SIM). 1,4-Phenylenediamine-2,3,5,6-d₄ was selected as an internal standard that was added at the beginning of the extraction procedure and used to correct for recovery and matrix effects. The linearity ranged from 1.0 to 1275μg/mL with a coefficient of determination (r²) greater than 0.999. LOQ and LOD were 1.0 and 0.10μg/mL, respectively. The recovery in a tattoo Product containing PPD was 94% and that for a tattoo Product containing no PPD reached 105%. Extraction efficiency of 98% was obtained. This method has been successfully applied to henna temporary tattoo and other henna-related Cosmetic Products for the determination and quantitation of PPD.
Anavella Gaitan Herrera - One of the best experts on this subject based on the ideXlab platform.
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Microbiological analysis of Cosmetics.
Methods in molecular biology (Clifton, N.J.), 2004Co-Authors: Anavella Gaitan HerreraAbstract:Cosmetics are Products of chemical or natural origin dedicated specifically for use in skin and mucosa. The constant development of the Cosmetic industry has generated the necessity to carry out microbiological analysis on the raw materials used in the industrial Production of Cosmetics as well as the final Products, with the purpose of obtaining Products of good microbiological quality. Cosmetic Products are recognized to be substrates for the survival and development of a large variety of microorganisms, since they possess some of the nutrients that facilitate growth such as: lipids, polysaccharides, alcohol, proteins, amino acids, glucosides, esteroids, peptides, and vitamins. Also, the conditions of readiness (oxygenation, pH, temperature, osmotic degree, superficial activity, perfume, and essential oils) present in the Cosmetic Products favor microbial multiplication. Routine analyses to determine the microbiological quality of a Cosmetic Product include the following: Count of mesophilic aerobic microorganisms. Most probable number (MPN) of total coliforms. Count of molds and yeasts. Absence/presence of Staphylococcus aureus probe. Absence/presence of Pseudomonas aeruginosa probe.
J.w. Kirn - One of the best experts on this subject based on the ideXlab platform.
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Determination of salicylate- and benzophenone-type sunscreen agents in Cosmetic Products by gas chromatography-mass spectrometry
Journal of Chromatography A, 1994Co-Authors: J.b. Choi, M H Lee, J.w. KirnAbstract:Abstract A novel simple method to detect salicylate- and benzophenone-type sunscreen agents in Cosmetic Products by gas chromatography-mass spectrometry (GC-MS) has been developed. Seven sunscreen agents (two salicylate-type and five benzophenone-type) were used for this study. Sunscreen agents and Cosmetic Product solutions were prepared by dissolving in dimethylformamide, and silylated with bis-trimethylsilyltrifluoroacetamide-trichloro-methylsilane (BSTFA). Silylated sunscreen agents were separated on a cross-linked methyl silicone gum column. The identification of each sunscreen agent was accomplished by retention time and mass spectrum library search with a computer, and the quantitation was made in the selected-ion monitoring (SIM) mode of GC-MS. Silylation increased the detection limits of all sunscreen agents about 20–170-fold. Linearity was maintained over the range 1–300 μg ml for each sunscreen agent. Each Cosmetic Product (i.e. sun lotion, sun cream and sun cream foundation) was found to contain amounts of the sunscreen agents. This method was sensitive and gave 95.2–104.1% recovery of each sunscreen agent from these Cosmetic Products. From these results, we concluded that silylation with BSTFA followed by GC-MS analysis allows the simple, convenient and exact determination of sunscreen agents from Cosmetic Products.
Stephanie Mathes - One of the best experts on this subject based on the ideXlab platform.
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Skin Models for Drug Development and Biopharmaceutical Industry
Skin Tissue Engineering and Regenerative Medicine, 2016Co-Authors: Heinz Ruffner, Ursula Graf-hausner, Stephanie MathesAbstract:Human skin models are widely used for safety testing of Cosmetic Product ingredients. These tissue models are also gaining increased importance for drug development in pharmaceutical research, as there is a significant potential for new compounds in the dermatological field. As other systems, such as xenografts, are deployable only for a limited number of investigations due to differences in permeability, hairiness, and occurrence of skin abnormalities, there is a strong demand for relevant and organotypic human in vitro skin models. In this chapter we present the most relevant skin diseases and appropriate models that have already been developed. We discuss prerequisites that are critical in drug development processes in the pharmaceutical industry such as model validation, gating studies to enable translation to clinical studies, and biological as well as system-dependent limitations. With the growing establishment of new Production techniques and increased knowledge of biological interactions, a new generation of human skin equivalents is emerging that facilitate drug development for dermatological applications.
Perry G Wang - One of the best experts on this subject based on the ideXlab platform.
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rapid determination of para phenylenediamine by gas chromatography mass spectrometry with selected ion monitoring in henna containing Cosmetic Products
Journal of Chromatography A, 2011Co-Authors: Perry G Wang, Alexander J. KrynitskyAbstract:A rapid method for the determination of para-phenylenediamine (PPD) in Cosmetic Products, such as henna tattoos has been developed and evaluated. This analytical procedure involved extracting a 10mg test portion of Cosmetic Product in 10mL of ethyl acetate, followed by determination by gas chromatography–mass spectrometry in the selected ion monitoring mode (GC/MS-SIM). 1,4-Phenylenediamine-2,3,5,6-d₄ was selected as an internal standard that was added at the beginning of the extraction procedure and used to correct for recovery and matrix effects. The linearity ranged from 1.0 to 1275μg/mL with a coefficient of determination (r²) greater than 0.999. LOQ and LOD were 1.0 and 0.10μg/mL, respectively. The recovery in a tattoo Product containing PPD was 94% and that for a tattoo Product containing no PPD reached 105%. Extraction efficiency of 98% was obtained. This method has been successfully applied to henna temporary tattoo and other henna-related Cosmetic Products for the determination and quantitation of PPD.
