The Experts below are selected from a list of 975 Experts worldwide ranked by ideXlab platform
Aparecido Almeida Conceição - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in Cottonseed Cake and fungal-treated Cottonseed Cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed Cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify Cottonseed Cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including Cottonseed Cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in Cottonseed Cake and fungal treated Cottonseed Cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed Cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify Cottonseed Cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including Cottonseed Cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol Cottonseed treated with a macrofungus.
S K Khare - One of the best experts on this subject based on the ideXlab platform.
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Secretome Analysis and Bioprospecting of Lignocellulolytic Fungal Consortium for Valorization of Waste Cottonseed Cake by Hydrolase Production and Simultaneous Gossypol Degradation
Waste and Biomass Valorization, 2019Co-Authors: Jasneet Grewal, Rameshwar Tiwari, S K KhareAbstract:PurposeThe utilisation of agro-wastes as feedstocks for biorefinery development is a promising strategy to combat the looming energy challenges with minimal environmental impact. Deoiled Cottonseed Cake is an abundant lignocellulosic residual agro-waste with restricted usage due to presence of toxic gossypol. The utilisation of this waste biomass for production of hydrolases (cellulase and xylanase) and simultaneous degradation of toxic gossypol was attempted by fungal mediated bioprocess development. The secretome profiling was carried out to gain insights into the diversity of proteins expressed by the fungal consortium under solid-state fermentation (SSF) conditions.MethodsThe three potential lignocellulolytic fungal strains, Aspergillus niger, Trichoderma reesei, Phanerochaete chrysosporium and their consortium were exploited for SSF of Cottonseed Cake. The secretome profiling was carried out by LC-ESI MS/MS based proteomic approach. The efficiency of hydrolases produced from seed Cake was verified by saccharification of wheat straw.ResultsThe consortium produced highest titre of cellulase (FPase: 21.62 IU; CMCase: 155.41 IU; β -glucosidase: 29.73 IU) and xylanase (2008.05 IU) per gram Cake with 90% reduction in gossypol content. The secretome analysis revealed versatile mixture of 81 proteins comprising of hemicellulases (36%), cellulases (17%), amylases (7%), esterases (6%), proteases (5%), hypothetical proteins (20%) and other proteins (9%). The Glycosyl hydrolase (GH) proteins constituted 73% of the total secretome. The high yield of reducing sugars (402.38 mg/g) was obtained from saccharification of wheat straw.ConclusionsThe present study highlights the sustainable valorization of an agro-waste by microbiological transformation for production of value-added products in biorefinery.
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2 pyrrolidone synthesis from γ aminobutyric acid produced by lactobacillus brevis under solid state fermentation utilizing toxic deoiled Cottonseed Cake
Bioprocess and Biosystems Engineering, 2017Co-Authors: Jasneet Grewal, S K KhareAbstract:There is an increasing demand of γ-aminobutyric acid (GABA) as drug and food additive, as well as feedstock to produce 2-pyrrolidone, a precursor for the synthesis of nylon 4. 2-Pyrrolidone is a petrochemical and depleting reserve which raises concern for its bio-based production. The study herein describes bio-based economical GABA production from Lactobacillus brevis by solid-state fermentation (SSF) using toxic deoiled Cottonseed Cake (CSC) as substrate. In general, the use of Cottonseed Cake remains restricted due to the presence of toxic gossypols. Thus, simultaneous detoxification observed during fermentation also widens the scope of utilization of this residual seedCake for feed use vis-a-vis production of other value added chemicals. The SSF conditions were optimized for maximum GABA production, viz., 19.7 mg/g, CSC of GABA was obtained at 6th day of fermentation with 70 % degradation of gossypols simultaneously. The potential of this bio-based GABA as a platform chemical is demonstrated in the synthesis of 2-pyrrolidone. Thus, a simple and cost-effective strategy for utilizing toxic biomass has been developed as an alternate to chemical synthetic route.
Simone Mendonça - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in Cottonseed Cake and fungal-treated Cottonseed Cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed Cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify Cottonseed Cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including Cottonseed Cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in Cottonseed Cake and fungal treated Cottonseed Cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed Cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify Cottonseed Cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including Cottonseed Cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol Cottonseed treated with a macrofungus.
Clemente Batista Soares Neto - One of the best experts on this subject based on the ideXlab platform.
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Development of an RP-UHPLC-PDA method for quantification of free gossypol in Cottonseed Cake and fungal-treated Cottonseed Cake.
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed Cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify Cottonseed Cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including Cottonseed Cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)
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development of an rp uhplc pda method for quantification of free gossypol in Cottonseed Cake and fungal treated Cottonseed Cake
PLOS ONE, 2018Co-Authors: Aparecido Almeida Conceição, Clemente Batista Soares Neto, José Antônio De Aquino Ribeiro, Félix Gonçalves De Siqueira, Robert N.g. Miller, Simone MendonçaAbstract:Cottonseed Cake biomass, which is a residue of oil extraction, is potentially appropriate for use as animal feed, given the high mineral, fibre and protein content. The presence of free gossypol, however, a toxic pigment in the glands of the cotton plant, limits use of this biomass for monogastric livestock. A promising method to detoxify Cottonseed Cake relies on fermentation by fungi, which can eliminate up to 100% of gossypol. In order to quantify trace levels of free gossypol in different cotton materials, including Cottonseed Cake treated with macrofungi, a simple and rapid chromatographic detection method was developed and validated. Under optimized conditions, extraction was performed using 70% acetone. The extract was then analysed by Ultra High-Performance Liquid Chromatography (UHPLC), with gradient elution on a C18 reverse phase column KINETEX® (100 x 2.10 mm, 2.6 μm). Methanol-0.1% TFA aqueous solution was employed as mobile phase and PDA detection conducted at 254 nm. The optimized method was validated by analysis of specificity, linearity and range, limit of detection, limit of quantification, precision and accuracy. Detection and quantification limits were observed at 0.2 and 0.5 μg/mL, respectively. With good reproducibility, with precision (RSD)<10% and recovery greater than 94%, the developed assay was appropriate for quantification of low quantities of free gossypol. The validated method was successfully applied to determine trace levels of free gossypol Cottonseed treated with a macrofungus.
O.a. Amao - One of the best experts on this subject based on the ideXlab platform.
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Histological Changes in Liver and Kidney of Rabbit Buck Fed Diet Containing Cottonseed Cake Supplemented with Vitamin E.
Journal of Natural Sciences Research, 2016Co-Authors: O.a. AmaoAbstract:Sixty-four (64) weanling rabbit bucks, aged 5-6 weeks with an average weight of 511.98g, were randomly allocated to eight treatment combinations comprising four levels (0, 5, 10 and 15%) of Cottonseed Cake (CSC) and two levels (0, 30mg / kg diet) of vitamin E supplementation with eight replicates in a 2x4 factorial experiment. Animals were fed the treatment diets for 20 weeks. At the end of the feeding trial, 3 rabbits per treatment were sacrificed and their internal organs including liver and kidneys were carefully dissected out. Samples of organs were fixed in 10% formaldehyde solution and later processed for histological assessment. It was observed that CSC induced various degrees of damage to the architecture of the liver and kidney of the rabbit bucks. Histological examination revealed a dose-dependent degeneration, cellular infiltration and necrosis of liver hepatocytes. At all levels of the CSC, vitamin E (30mg/kg diet) corrected the damages done to the liver. Mild degenerative changes were observed for the kidney structure at 5% and 10% CSC levels. The renal degenerative changes became severe at 15% CSC inclusion level. Vitamin E supplementation ameliorated these adverse effects. Thus, vitamin E conferred some protection on the liver and kidney against the adverse effects of the CSC. It was concluded that if Cottonseed Cake is included in diets of rabbits at any level, it should be adequately supplemented with vitamin E. Keywords : Rabbit buck, Liver, Kidney, Histology, Cottonseed Cake, Vitamin E.
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testicular and epididymal characteristcs of rabbit bucks fed diets containing Cottonseed Cake supplemented with carrot daucus carota or ginger zingiber officinale roscoe
Journal of Biology Agriculture and Healthcare, 2016Co-Authors: O.a. Amao, P O OladeleAbstract:This experiment was conducted to investigate the reproductive characteristics of rabbit bucks fed Cottonseed Cake-based diets supplemented with carrot or ginger. Twenty four (24) cross bred (New Zealand White X Chinchilla) 6-8 weeks old rabbit bucks were involved in the study. The bucks were balanced for weight and allocated to four dietary treatments T1, T2, T3 and T4. Treatment T1 (control) contained soyabean meal (SBM) as the main plant protein source denoted as (SBM or 0% CSC). T2 had 100% SBM replaced with Cottonseed Cake (CSC), while T3 and T4 had T2 supplemented with carrot and ginger respectively. Except for testicular width, testicular characteristics were not significantly (P>0.05) affected. Testicular width decreased from 2.20cm in the control (T1) to 1.63cm in T2 (100% CSC). Mean epididymal weight and mean epididymal length were significantly (P<0.05) affected with mean epididymal length decreasing from 13.72cm in the control to 8.32cm in T2 (100% CSC). Supplementing T2 with either carrot or ginger increased the epididymal characteristics significantly (P<0.05). It could be concluded from this study that CSC with or without carrot or ginger supplementation may not have adverse effect on testicular characteristics. However CSC depressed epididymal characteristics which were corrected by carrot or ginger supplementation with ginger proving more effective. Keywords : Rabbit buck, Reproductive characteristics, Cottonseed Cake, Carrot and Ginger.
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Testicular and Epididymal Characteristcs of Rabbit Bucks Fed Diets Containing Cottonseed Cake Supplemented with Carrot (Daucus carota) or Ginger (Zingiber officinale Roscoe)
Journal of Biology Agriculture and Healthcare, 2016Co-Authors: O.a. Amao, P O OladeleAbstract:This experiment was conducted to investigate the reproductive characteristics of rabbit bucks fed Cottonseed Cake-based diets supplemented with carrot or ginger. Twenty four (24) cross bred (New Zealand White X Chinchilla) 6-8 weeks old rabbit bucks were involved in the study. The bucks were balanced for weight and allocated to four dietary treatments T1, T2, T3 and T4. Treatment T1 (control) contained soyabean meal (SBM) as the main plant protein source denoted as (SBM or 0% CSC). T2 had 100% SBM replaced with Cottonseed Cake (CSC), while T3 and T4 had T2 supplemented with carrot and ginger respectively. Except for testicular width, testicular characteristics were not significantly (P>0.05) affected. Testicular width decreased from 2.20cm in the control (T1) to 1.63cm in T2 (100% CSC). Mean epididymal weight and mean epididymal length were significantly (P
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Gonadal and Extra Gonadal Sperm Characteristics of Rabbit Bucks Fed Cottonseed Cake Based Diets Supplemented With Vitamin E
Journal of Animal Science Advances, 2012Co-Authors: O.a. Amao, Victor Adegboye Togun, David Olusoji AdejumoAbstract:The aim of this study was to investigate the effect on sperm characteristics of rabbit bucks fed Cottonseed Cake based diets supplemented with vitamin E. Sixty–four (64) weanling rabbit bucks, aged 5 – 6 weeks, with an average weight of 511.28g, were randomly allocated to eight treatment combinations comprising four levels (0, 5, 10 and 15%) of Cottonseed Cake (CSC) and two levels (0, and 30 mg / kg diet) of vitamin E supplementation, with eight replicates in a 2 x 4 factorial arrangement within a completely randomized design experiment. Animals were first fed the CSC-based diets for eight weeks in a pre-pubertal trial, after which the diets were slightly adjusted to suit their growing phase. They were then fed the test diets for another 12 weeks. At the end of this trial, 3 rabbits per treatment were slaughtered and their reproductive organs dissected out for gonadal and extra-gonadal sperm assessment. Gonadal sperm reserve (GSR), daily sperm production (DSP) and daily sperm production per gram testis (DSP/g) for the bucks fed 5, 10 and 15% CSC without vitamin E supplementation were significantly (P
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Testicular Morphometry and Histological Changes in Rabbit Bucks Fed Cottonseed Cake-Based Diets Supplemented with Vitamin E -
Journal of Animal Science Advances, 2012Co-Authors: O.a. Amao, David Olusoji Adejumo, Victor Adegboye TogunAbstract:Sixty-four(64) weanling rabbit bucks, aged 5-6 weeks and averagely weighing 511.98g, were randomly allocated to eight treatment combinations comprising four levels (0, 5, 10 and 15%) of Cottonseed Cake (CSC) and two levels (0, 30mg / kg diet) of vitamin E supplementation with eight replicates in a 2x4 factorial experiment. Animals were fed the treatment diets for a total of 20 weeks. At the end of the feeding trial, 3 rabbits per treatment were slaughtered and their reproductive organs carefully dissected out; separated into different components and measured. Left testis was fixed in Bouin’s fixative and later processed for histological assessment. Data on testicular morphometry were analyzed by ANOVA using the General Linear Model (GLM) appropriate for a 2 x 4 factorial experiment. Paired testis weight was significantly (P 0.05) influenced by the CSC and vitamin E interaction. Paired epididymal weight was not significantly (P>0.05) affected by the interaction. However, at 0% CSC, paired epididymal length was significantly (P
