The Experts below are selected from a list of 30534 Experts worldwide ranked by ideXlab platform
Suzanne Fredericq - One of the best experts on this subject based on the ideXlab platform.
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Characterization and phylogenetic affinities of the red alga Chondrophycus flagelliferus (Rhodomelaceae‚ Ceramiales) from Brazil on the basis of morphological and molecular evidence
Phycologia, 2006Co-Authors: Mutue T. Fujii, Silvia M. P. B. Guimarães, Carlos Frederico D. Gurgel, Suzanne FredericqAbstract:Abstract A detailed study of the vegetative and reproductive morphology ofChondrophycus flagelliferus from Brazil is provided. The species possesses axial segments, each bearing two periaxial Cells, a situation characteristic for the genusChondrophycus. WithinChondrophycus,C. flagelliferus belongs to the subgenusPalisadi, sectionPalisadi, on the basis of the presence of a palisade-like outer cortical Cell layer as seen in transverse sections of branchlets; the absence of secondary pit connections between cortical Cells; the fertile periaxial Cell with two pre-sporangial Cover Cells, the tetrasporangium initial and the post-sporangial Cover Cell that will develop into the corticating system; and the right-angled tetrasporangial arrangement. The phylogenetic position of this species withinLaurenciasensu lato is inferred from parsimony and Bayesian analyses of chloroplast-encodedrbcL sequences from 39 Rhodomelaceae using two Ceramiaceae as the out-group. This study corroborates the taxonomic decision to spli...
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Nitophyllum hommersandii sp. nov. (Delesseriaceae, Rhodophyta) from Taiwan
European Journal of Phycology, 2003Co-Authors: Showe-mei Lin, Suzanne FredericqAbstract:A new member of the Delesseriaceae (Ceramiales, Rhodophyta) is described from Kenting National Park, southern Taiwan. On the basis of comparative vegetative and reproductive morphology, and phylogenetic analysis inferred from chloroplast- encoded rbcL sequences, we conclude that it belongs in the genus Nitophyllum, tribe Nitophylleae, subfamily Nitophylloideae. The new taxon shares the following features with the type species of Nitophyllum, N. punctatum (Stackhouse) Greville: the absence of macro- and microscopic veins; diffuse growth by marginal and intercalary multinucleate meristematic Cells; discoid chloroplasts; mature procarps scattered along the thallus margin on both sides, each consisting of a supporting Cell bearing a straight, 4-Celled carpogonial branch flanked laterally by a Cover Cell and one sterile group, two connecting Cells cut off from the fertilized carpogonium, an auxiliary Cell cut off apically from the supporting Cell and diploidized by one of two connecting Cells; gonimoblasts irr...
Showe-mei Lin - One of the best experts on this subject based on the ideXlab platform.
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Nitophyllum hommersandii sp. nov. (Delesseriaceae, Rhodophyta) from Taiwan
European Journal of Phycology, 2003Co-Authors: Showe-mei Lin, Suzanne FredericqAbstract:A new member of the Delesseriaceae (Ceramiales, Rhodophyta) is described from Kenting National Park, southern Taiwan. On the basis of comparative vegetative and reproductive morphology, and phylogenetic analysis inferred from chloroplast- encoded rbcL sequences, we conclude that it belongs in the genus Nitophyllum, tribe Nitophylleae, subfamily Nitophylloideae. The new taxon shares the following features with the type species of Nitophyllum, N. punctatum (Stackhouse) Greville: the absence of macro- and microscopic veins; diffuse growth by marginal and intercalary multinucleate meristematic Cells; discoid chloroplasts; mature procarps scattered along the thallus margin on both sides, each consisting of a supporting Cell bearing a straight, 4-Celled carpogonial branch flanked laterally by a Cover Cell and one sterile group, two connecting Cells cut off from the fertilized carpogonium, an auxiliary Cell cut off apically from the supporting Cell and diploidized by one of two connecting Cells; gonimoblasts irr...
Hanno Steen - One of the best experts on this subject based on the ideXlab platform.
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Optimization of Cell Lysis and Protein Digestion Protocols for Protein Analysis by LC-MS/MS
Methods in molecular biology (Clifton N.J.), 2015Co-Authors: Dominic Winter, Alireza Dehghani, Hanno SteenAbstract:Mass spectrometry-based proteomics is the method of choice for analyzing (sub-) proteomes from virtually any source; with Cells grown in culture being the most frequently investigated samples. Using HeLa Cells, we describe a strategy for the optimization of protocols for whole proteome analysis. We Cover Cell lysis, protein precipitation and digestion, comparing the results obtained using different parameters and offering various possibilities for the optimization of a proteomic workflow which can be used for virtually any type of animal Cell grown in culture.
Brad J. Berron - One of the best experts on this subject based on the ideXlab platform.
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Hydrogel Patches on Live Cells through Surface-Mediated Polymerization.
Langmuir : the ACS journal of surfaces and colloids, 2017Co-Authors: Jacob L. Lilly, Roberto Arreaza, Brad J. BerronAbstract:Many naturally occurring Cells possess an intrinsic ability to cross biological barriers that block conventional drug delivery, and these Cells offer a possible mode of active transport across the blood–brain barrier or into the core of tumor masses. While many technologies for the formation of complete, nanoparticle-loaded coatings on Cells exist, a complete coating on the Cell surface would disrupt the interaction of Cells with their environments. To address this issue, Cell surface patches that partially Cover Cell surfaces might provide a superior approach for Cell-mediated therapeutic delivery. The goal of this study is to establish a simplified approach to producing polymeric patches of arbitrary shapes on a live Cell via surface-mediated photopolymerization. Cell surfaces were nonspecifically labeled with eosin, and polyethylene (glycol) diacrylate (PEGDA) coatings were directed to specific sites using 530 nm irradiation through a chrome-coated photomask. These coatings may entrap drug-loaded or im...
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Hydrogel Patches on Live Cells through Surface-Mediated Polymerization
2017Co-Authors: Jacob L. Lilly, Roberto Arreaza, Brad J. BerronAbstract:Many naturally occurring Cells possess an intrinsic ability to cross biological barriers that block conventional drug delivery, and these Cells offer a possible mode of active transport across the blood–brain barrier or into the core of tumor masses. While many technologies for the formation of complete, nanoparticle-loaded coatings on Cells exist, a complete coating on the Cell surface would disrupt the interaction of Cells with their environments. To address this issue, Cell surface patches that partially Cover Cell surfaces might provide a superior approach for Cell-mediated therapeutic delivery. The goal of this study is to establish a simplified approach to producing polymeric patches of arbitrary shapes on a live Cell via surface-mediated photopolymerization. Cell surfaces were nonspecifically labeled with eosin, and polyethylene (glycol) diacrylate (PEGDA) coatings were directed to specific sites using 530 nm irradiation through a chrome-coated photomask. These coatings may entrap drug-loaded or imaging particles. The extent of nonspecific formation of PEGDA hydrogel coatings increased with irradiation time, light intensity, and initiating species; 40 mW/cm2 irradiation for 5 min delivered high-resolution patterns on the surface of A549 Cells, and these Cells remained viable for 48 h postpatterning with fluorescent nanoparticle-loaded coatings. This work first demonstrated the feasibility of photopatterning polymer patches directly on the surface of Cells
Michael R. Frater - One of the best experts on this subject based on the ideXlab platform.
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a Cell loss concealment technique for mpeg 2 coded video
IEEE Transactions on Circuits and Systems for Video Technology, 2000Co-Authors: Jian Zhang, John F. Arnold, Michael R. FraterAbstract:Audio-visual and other multimedia services are seen as important sources of traffic for future telecommunication networks, including wireless networks. A major drawback with some wireless networks is that they introduce a significant number of transmission errors into the digital bitstream. For video, such errors can have the effect of degrading the quality of service to the point where it is unusable. We introduce a technique that allows for the concealment of the impact of these errors. Our work is based on MPEG-2 encoded video transmitted over a wireless network whose data structures are similar to those of asynchronous transfer mode (ATM) networks. Our simulations include the impact of the MPEG-2 systems layer and Cover Cell-loss rates up to 5%. This is substantially higher than those that have been discussed in the literature up to this time. We demonstrate that our new approach can significantly increase received video quality, but at the cost of a considerable computational overhead. We then extend our technique to allow for higher computational efficiency and demonstrate that a significant quality improvement is still possible.
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Video error concealment using decoder motion vector estimation
TENCON '97 Brisbane - Australia. Proceedings of IEEE TENCON '97. IEEE Region 10 Annual Conference. Speech and Image Technologies for Computing and Tel, 1Co-Authors: Jian Zhang, John F. Arnold, Michael R. Frater, Mark R. PickeringAbstract:Audio-visual and other multimedia services are seen as important sources of traffic for future wireless networks. An important characteristic of such networks is that they introduce a significant number of errors into the transmitted digital bitstream. For services' such as video, these errors can have the effect of degrading the quality of the received service to the point where it is unusable. We introduce a technique that allows the concealment of the impact of these errors. Our work is based on MPEG 2 encoded video (although our scheme would work equally well with other standards) to be transmitted over a wireless network whose data structures are similar to those of asynchronous transfer mode (ATM). Our simulations include the impact of the MPEG 2 Systems Layer and Cover Cell loss rates up to 5%.
