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Ibrahim Elsohaby - One of the best experts on this subject based on the ideXlab platform.
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assessment of brix refractometry to estimate immunoglobulin g concentration in beef Cow Colostrum
Journal of Veterinary Internal Medicine, 2020Co-Authors: Lisa Gamsjäger, Ibrahim Elsohaby, Deborah M Haines, Jennifer M Pearson, Michel Levy, Edmond A. Pajor, Claire M WindeyerAbstract:BACKGROUND Brix refractometry can be used to assess colostral immunoglobulin G (IgG) concentration, but studies identifying Brix percentages to detect high- and low-IgG Colostrum are lacking for beef Cows and interlaboratory agreement is unknown. OBJECTIVES Evaluate Brix refractometer performance and interlaboratory agreement for assessing beef Cow Colostrum IgG concentration, including determination of thresholds to identify Colostrum containing IgG concentrations <100 g/L and ≥150 g/L. ANIMALS Beef Cows (n = 416) from 11 Cow-calf operations in Alberta, Canada. METHODS Colostral IgG concentrations were measured using radial immunodiffusion (RID) and estimated by Brix refractometry for this retrospective study. Spearman correlation coefficients were assessed between RID and Brix refractometry. Likelihood ratios and misclassification cost-term analysis were used to determine optimal Brix percentages for detecting Colostrum containing IgG concentrations <100 g/L and ≥150 g/L. Concordance correlation coefficient (CCC) and Bland-Altman analyses were performed for Brix percentages obtained at 3 different laboratories. RESULTS Brix percentages obtained at 3 laboratories were positively correlated with IgG results (r = 0.72, 0.68, and 0.76, respectively). Colostrum Brix percentages of <24% and ≥30% were optimal for indicating IgG concentrations of <100 g/L and ≥150 g/L, respectively. Interlaboratory agreement was substantial, with CCC ranging from 0.89 to 0.96 and Bland-Altman analysis showing small mean differences (-1.2% to 0.09% Brix) and narrow limits of agreements (-4.8% to 2.4% Brix) among laboratories. CONCLUSIONS AND CLINICAL IMPORTANCE Brix refractometry shows good potential for reliably estimating IgG concentrations in beef Cow Colostrum across multiple laboratories and can be recommended to aid Colostrum management decisions on farms.
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Assessment of Brix refractometry to estimate immunoglobulin G concentration in beef Cow Colostrum.
Journal of veterinary internal medicine, 2020Co-Authors: Lisa Gamsjäger, Ibrahim Elsohaby, Deborah M Haines, Jennifer M Pearson, Michel Levy, Edmond A. Pajor, M. Claire WindeyerAbstract:BACKGROUND Brix refractometry can be used to assess colostral immunoglobulin G (IgG) concentration, but studies identifying Brix percentages to detect high- and low-IgG Colostrum are lacking for beef Cows and interlaboratory agreement is unknown. OBJECTIVES Evaluate Brix refractometer performance and interlaboratory agreement for assessing beef Cow Colostrum IgG concentration, including determination of thresholds to identify Colostrum containing IgG concentrations
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application of transmission infrared spectroscopy and partial least squares regression to predict immunoglobulin g concentration in dairy and beef Cow Colostrum
Journal of Animal Science, 2018Co-Authors: Ibrahim Elsohaby, Claire M Windeyer, Deborah M Haines, Elizabeth R Homerosky, Jennifer M Pearson, Trenton J Mcclure, Greg P KeefeAbstract:The objective of this study was to explore the potential of transmission infrared (TIR) spectroscopy in combination with partial least squares regression (PLSR) for quantification of dairy and beef Cow colostral immunoglobulin G (IgG) concentration and assessment of Colostrum quality. A total of 430 Colostrum samples were collected from dairy (n = 235) and beef (n = 195) Cows and tested by a radial immunodiffusion (RID) assay and TIR spectroscopy. Colostral IgG concentrations obtained by the RID assay were linked to the preprocessed spectra and divided into combined and prediction data sets. Three PLSR calibration models were built: one for the dairy Cow Colostrum only, the second for beef Cow Colostrum only, and the third for the merged dairy and beef Cow Colostrum. The predictive performance of each model was evaluated separately using the independent prediction data set. The Pearson correlation coefficients between IgG concentrations as determined by the TIR-based assay and the RID assay were 0.84 for dairy Cow Colostrum, 0.88 for beef Cow Colostrum, and 0.92 for the merged set of dairy and beef Cow Colostrum. The average of the differences between colostral IgG concentrations obtained by the RID- and TIR-based assays were -3.5, 2.7, and 1.4 g/L for dairy, beef, and merged Colostrum samples, respectively. Further, the average relative error of the colostral IgG predicted by the TIR spectroscopy from the RID assay was 5% for dairy Cow, 1.2% for beef Cow, and 0.8% for the merged data set. The average intra-assay CV% of the IgG concentration predicted by the TIR-based method were 3.2%, 2.5%, and 6.9% for dairy Cow, beef Cow, and merged data set, respectively.The utility of TIR method for assessment of Colostrum quality was evaluated using the entire data set and showed that TIR spectroscopy accurately identified the quality status of 91% of dairy Cow Colostrum, 95% of beef Cow Colostrum, and 89% and 93% of the merged dairy and beef Cow Colostrum samples, respectively. The results showed that TIR spectroscopy demonstrates potential as a simple, rapid, and cost-efficient method for use as an estimate of IgG concentration in dairy and beef Cow Colostrum samples and assessment of Colostrum quality. The results also showed that merging the dairy and beef Cow Colostrum sample data sets improved the predictive ability of the TIR spectroscopy.
S.t. Fernando - One of the best experts on this subject based on the ideXlab platform.
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Toxocara vitulorum: maternal transfer of antibodies from buffalo Cows (Bubalis bubalis) to calves and levels of infection with T vitulorum in the calves.
Research in veterinary science, 1994Co-Authors: R.p.v.j. Rajapakse, S. Lloyd, S.t. FernandoAbstract:The levels of antibody to the excretory/secretory antigens of the infective larvae and adults of Toxocara vitulorum were measured by gel precipitation and ELISAs in the serum and Colostrum of 12 buffalo Cows naturally infected with T vitulorum and in the serum of their calves. The antibody levels were compared with the extent of T vitulorum infection as judged by faecal egg counts in the calves. The patterns of bands of the larval antigens and gel precipitating antibodies in Cow serum taken one month before calving, in Cow Colostrum and in calf serum were very similar. Nine Cows and their calves had gel precipitating antibodies but the remaining three Cows and their calves did not. The ELISA detected anti-larval antibodies in the Colostrum of all 12 Cows and calves. With the exception of one calf there was a strong correlation (r = 0.902) between the antibody titre in Cow Colostrum and the titre of passively acquired antibody in calf serum. The titres of these passively acquired antibodies declined to their lowest levels in calves 12 to 25 days of age; the antibody concentrations then began to increase up to day 42 and remained stable for the remainder of the experiment (105 days). The titres of antibodies to the antigens of the adult worms, examined in four Cows and their calves, were lower than the titres to the larval antigens; the calves absorbed this anti-adult antibody from the Colostrum and the antibody levels reached a plateau between days 12 and 30 and remained stable for the rest of the experiment.(ABSTRACT TRUNCATED AT 250 WORDS)
Takusaburo Ebina - One of the best experts on this subject based on the ideXlab platform.
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production of anti hair keratin antibody and its characteristics utilization of Cow Colostrum and milk
Nihon Chikusan Gakkaiho, 1994Co-Authors: Takusaburo Ebina, Hideyo Uchiwa, Minoru Ohta, Umeji MurakamiAbstract:皮膚ケラチンとは反応せず,毛髪に特異的に結合し,毛髪の破断強度を上げる抗毛髪ケラチ ン抗体を含む初乳ならびに常乳を免疫牛に産生させた.人毛髪ケラチン50mgを出産60日前のホルスタイン牛に筋肉内に注射し,(2～3)週間後に同抗原を乳房内または乳房リンパ節附近に投与すると,血中抗体価ならびに初乳中の毛髪ケラチンに対する抗体価は105に上昇した.この時,免疫牛一頭の初乳中に(1～1.5)kgのIgGを産生することが出来た.さらにこれらの免疫牛に分娩2～3ヵ月後ケラチンを50mgずつ筋肉注射し,4週おきに同抗原を筋肉内液射又は腹腔内注射を3回行なったところ,常乳中に初乳と同等の比活性を持った抗体が出来るにとを見出した.常乳では300日間搾乳することができ,全体では(4～5)kgのIgGを得ることができる.にれより,初乳に加えて常乳を抗体生産に利用するにとが可能となった.
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Passive immunizations of suckling mice and infants with bovine Colostrum containing antibodies to human rotavirus.
Journal of Medical Virology, 1992Co-Authors: Takusaburo Ebina, Minoru Ohta, Yoshihiro Kanamaru, Yoshiko Yamamoto-osumi, Koichi BabaAbstract:After immunizing 8-month pregnant Holstein Cows with the human rotavirus MO strain, Cow Colostrum containing neutralizing antibody to four different serotypes of human rotavirus, designated Rota Colostrum, was obtained. Oral inoculation of human rotavirus MO strain into 5-day-old BALB/c mice causes gastroenteritis characterized by diarrhea. Using this small animal model, passive protection of suckling mice against human rotavirus infection was achieved with the use of Rota Colostrum. Rota Colostrums completely protected against rotavirus infection, but purified IgG and IgA obtained from Rota Colostrum were unable to protect against infection. After grouping randomly 20 infants from a baby care center, 10 infants received 20 ml of Rota Colostrum per day for 2 weeks and 10 control infants did not. Rotavirus-associated diarrhea developed in 7 of 10 infants in the control group. None of the three infants in the every day recipient group of Rota Colostrum had such symptoms, and one of three infants in the every other day recipient group developed rotavirus-induced diarrhea. All four infants who received Rota Colostrum after symptoms appeared developed diarrhea. Oral administration of Rota Colostrum seems to be an effective and safe means of preventing diarrhea caused by human rotavirus infection. © 1992 Wiley-Liss, Inc.
Lisa Gamsjäger - One of the best experts on this subject based on the ideXlab platform.
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assessment of brix refractometry to estimate immunoglobulin g concentration in beef Cow Colostrum
Journal of Veterinary Internal Medicine, 2020Co-Authors: Lisa Gamsjäger, Ibrahim Elsohaby, Deborah M Haines, Jennifer M Pearson, Michel Levy, Edmond A. Pajor, Claire M WindeyerAbstract:BACKGROUND Brix refractometry can be used to assess colostral immunoglobulin G (IgG) concentration, but studies identifying Brix percentages to detect high- and low-IgG Colostrum are lacking for beef Cows and interlaboratory agreement is unknown. OBJECTIVES Evaluate Brix refractometer performance and interlaboratory agreement for assessing beef Cow Colostrum IgG concentration, including determination of thresholds to identify Colostrum containing IgG concentrations <100 g/L and ≥150 g/L. ANIMALS Beef Cows (n = 416) from 11 Cow-calf operations in Alberta, Canada. METHODS Colostral IgG concentrations were measured using radial immunodiffusion (RID) and estimated by Brix refractometry for this retrospective study. Spearman correlation coefficients were assessed between RID and Brix refractometry. Likelihood ratios and misclassification cost-term analysis were used to determine optimal Brix percentages for detecting Colostrum containing IgG concentrations <100 g/L and ≥150 g/L. Concordance correlation coefficient (CCC) and Bland-Altman analyses were performed for Brix percentages obtained at 3 different laboratories. RESULTS Brix percentages obtained at 3 laboratories were positively correlated with IgG results (r = 0.72, 0.68, and 0.76, respectively). Colostrum Brix percentages of <24% and ≥30% were optimal for indicating IgG concentrations of <100 g/L and ≥150 g/L, respectively. Interlaboratory agreement was substantial, with CCC ranging from 0.89 to 0.96 and Bland-Altman analysis showing small mean differences (-1.2% to 0.09% Brix) and narrow limits of agreements (-4.8% to 2.4% Brix) among laboratories. CONCLUSIONS AND CLINICAL IMPORTANCE Brix refractometry shows good potential for reliably estimating IgG concentrations in beef Cow Colostrum across multiple laboratories and can be recommended to aid Colostrum management decisions on farms.
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Assessment of Brix refractometry to estimate immunoglobulin G concentration in beef Cow Colostrum.
Journal of veterinary internal medicine, 2020Co-Authors: Lisa Gamsjäger, Ibrahim Elsohaby, Deborah M Haines, Jennifer M Pearson, Michel Levy, Edmond A. Pajor, M. Claire WindeyerAbstract:BACKGROUND Brix refractometry can be used to assess colostral immunoglobulin G (IgG) concentration, but studies identifying Brix percentages to detect high- and low-IgG Colostrum are lacking for beef Cows and interlaboratory agreement is unknown. OBJECTIVES Evaluate Brix refractometer performance and interlaboratory agreement for assessing beef Cow Colostrum IgG concentration, including determination of thresholds to identify Colostrum containing IgG concentrations
S Tzipori - One of the best experts on this subject based on the ideXlab platform.
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hyperimmune Cow Colostrum reduces diarrhoea due to rotavirus a double blind controlled clinical trial
Acta Paediatrica, 2008Co-Authors: Amal K Mitra, Dilip Mahalanabis, H Ashraf, Leanne Unicomb, R Eeckels, S TziporiAbstract:The therapeutic efficacy of hyperimmune bovine Colostrum (HBC) from Cows immunized with four serotypes of human rotavirus was evaluated in a double-blind, randomized trial in 75 boys, aged 6-24 months, infected with rotavirus diarrhoea. The treatment group received 100 ml of HBC three times a day for 3 consecutive days, while the controls received the same amount of bovine Colostrum from significantly shorter duration of diarrhoea than the controls (median 56 versus 72 h (p<0.001); confidence interval of median difference (CI) 8-32 h). Total stool output (g/kg) between admission and cessation of diarrhoea was reduced by 29% in the HBC-treated group compared with controls (median 205 versus 290 g (p=0.04); CI = 1-154 g). In 50% of the children in the study group, diarrhoea stopped by 48 h, whereas 100% of the controls were still suffering from diarrhoea. No untoward effects were noted in either group. Colostrum from Cows immunized with rotavirus antigen is clinically effective in reducing the duration and severity of childhood diarrhoea due to rotavirus.
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Hyperimmune Cow Colostrum reduces diarrhoea due to rotavirus: a double-blind, controlled clinical trial.
Acta paediatrica (Oslo Norway : 1992), 2008Co-Authors: Amal K Mitra, Dilip Mahalanabis, H Ashraf, Leanne Unicomb, R Eeckels, S TziporiAbstract:The therapeutic efficacy of hyperimmune bovine Colostrum (HBC) from Cows immunized with four serotypes of human rotavirus was evaluated in a double-blind, randomized trial in 75 boys, aged 6-24 months, infected with rotavirus diarrhoea. The treatment group received 100 ml of HBC three times a day for 3 consecutive days, while the controls received the same amount of bovine Colostrum from significantly shorter duration of diarrhoea than the controls (median 56 versus 72 h (p
