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Lingfeng Kong - One of the best experts on this subject based on the ideXlab platform.

  • the effect of interstrain hybridization on the production performance in the pacific oyster Crassostrea Gigas
    Aquaculture, 2017
    Co-Authors: Lingfeng Kong, Shengliang Song
    Abstract:

    Abstract Intra-specific hybridization was carried out using three strains (C, J and K) of Crassostrea Gigas , which were successively mass selected for two generation from three culture stocks collected from China, Japan and South Korea. Detailed comparison on larval growth development and the growth and survival of the spats and adult were carried out among six hybrids and three parental groups. By way of the serial growth traits measurements (shell height, shell length and the whole body weight), the obtained results reveal that six hybrid crosses were inferior to those of parental crosses to different extend at larval stage, while at spat and adult stages some differences were observe. It was noted that the cross CJ (C♀ × J♂) outperformed all the other groups in all growth traits ( P P P Statement of relevance Three different culture stains of Crassostrea Gigas , which were successively selected for two generations, were evaluated for growth and fitness traits, as well as for their reciprocal crosses, to determine if heterosis exists in growth and survival traits at different stages by rearing them under the same conditions. Our results could have significant implications for the development of oyster aquaculture.

  • comparative transcriptome analysis of the pacific oyster Crassostrea Gigas characterized by shell colors identification of genetic bases potentially involved in pigmentation
    PLOS ONE, 2015
    Co-Authors: Dandan Feng, Xuelin Zhao, Lingfeng Kong
    Abstract:

    Background Shell color polymorphisms of Mollusca have contributed to development of evolutionary biology and population genetics, while the genetic bases and molecular mechanisms underlying shell pigmentation are poorly understood. The Pacific oyster (Crassostrea Gigas) is one of the most important farmed oysters worldwide. Through successive family selection, four shell color variants (white, golden, black and partially pigmented) of C. Gigas have been developed. To elucidate the genetic mechanisms of shell coloration in C. Gigas and facilitate the selection of elite oyster lines with desired coloration patterns, differentially expressed genes (DEGs) were identified among the four shell color variants by RNA-seq.

  • transcriptomic responses to salinity stress in the pacific oyster Crassostrea Gigas
    PLOS ONE, 2012
    Co-Authors: Xuelin Zhao, Lingfeng Kong
    Abstract:

    Background Low salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the Pacific oyster Crassostrea Gigas is considered to be tolerant to relative low salinity. The genes that regulate C. Gigas responses to osmotic stress were monitored using the next-generation sequencing of whole transcriptome with samples taken from gills. By RNAseq technology, transcript catalogs of up- and down-regulated genes were generated from the oysters exposed to low and optimal salinity seawater.

Henri Grizel - One of the best experts on this subject based on the ideXlab platform.

  • interactions between bonamia ostreae protozoa ascetospora and hemocytes of ostrea edulis and Crassostrea Gigas mollusca bivalvia in vitro system establishment
    Journal of Invertebrate Pathology, 1992
    Co-Authors: Chantal Mourton, Eric Mialhe, Dominique Chagot, Dominique Hervio, Evelyne Bachere, Viviane Boulo, Henri Grizel
    Abstract:

    Abstract Based on parasite purification and hemocyte primary culture, an in vitro system was established for studying early interactions between Bonamia ostreae and the hemocytes of sensitive Ostrea edulis and resistant Crassostrea Gigas. Infections were observed whichever the hemocytic types and the oyster species. The advantages and disadvantages of the system are discussed with regard to the study of recognition, entry, and survival mechanisms of the parasite.

  • interactions between bonamia ostreae protozoa ascetospora and hemocytes of ostrea edulis and Crassostrea Gigas mollusca bivalvia entry mechanisms
    Journal of Invertebrate Pathology, 1992
    Co-Authors: Dominique Chagot, Eric Mialhe, Chantal Mourton, Dominique Hervio, Evelyne Bachere, Viviane Boulo, Henri Grizel
    Abstract:

    Entry mechanisms of the intrahemocytic parasite Bonamia ostreae (Ascetospora) into hemocytes of the sensitive (Ostrea edulis) and a resistant (Crassostrea Gigas) oyster species have been analyzed. The study was based upon the development of a B. ostreae-hemocyte in vitro system. Ultrastructural features and Cytochalasin B sensitivity of the phenomenon demonstrate that B. ostreae enters into the two oyster species hemocytes by host-specified phagocytosis, although parasite contribution to entry into O. edulis hemocytes cannot be discarded. Possible receptors and the postphagocytic fate of the parasite are discussed.

  • Introduction into France of the Japanese oyster ( Crassostrea Gigas )
    ICES Journal of Marine Science, 1991
    Co-Authors: Henri Grizel, Maurice Heral
    Abstract:

    The Japanese oyster (Crassostrea Gigas) has been introduced in France on a large scale to replace cultivation of the Portuguese oyster (Crassostrea angulata), affected by a viral disease. The importation took place from 1971 to 1975, with broodstock coming from British Columbia (Canada) and spat from Japan. Good growth rate and success of the natural setting on the French Atlantic coast attested to the success of this implantation, with a production which reached 80000 t by 1976. The precautionary measures associated with this transfer Iimited the implantation of an introduced fauna, 15 years after, to: Balanus amphitrite, Aiptasia pulchella and, on the Mediterranean coast, Undaria pinnatifida and Laminaria japonica. The authors also discuss the possible role of Crassostrea Gigas in spreading the viral disease.

Guofan Zhang - One of the best experts on this subject based on the ideXlab platform.

  • identification and functional characterization of the glycogen synthesis related gene glycogenin in pacific oysters Crassostrea Gigas
    Journal of Agricultural and Food Chemistry, 2017
    Co-Authors: Jie Meng, Sheng Liu, Ting Wang, Ting Wang, Guofan Zhang
    Abstract:

    High glycogen levels in the Pacific oyster (Crassostrea Gigas) contribute to its flavor, quality, and hardiness. Glycogenin (CgGN) is the priming glucosyltransferase that initiates glycogen biosynthesis. We characterized the full sequence and function of C. Gigas CgGN. Three CgGN isoforms (CgGN-α, β, and γ) containing alternative exon regions were isolated. CgGN expression varied seasonally in the adductor muscle and gonadal area and was the highest in the adductor muscle. Autoglycosylation of CgGN can interact with glycogen synthase (CgGS) to complete glycogen synthesis. Subcellular localization analysis showed that CgGN isoforms and CgGS were located in the cytoplasm. Additionally, a site-directed mutagenesis experiment revealed that the Tyr200Phe and Tyr202Phe mutations could affect CgGN autoglycosylation. This is the first study of glycogenin function in marine bivalves. These findings will improve our understanding of glycogen synthesis and accumulation mechanisms in mollusks. The data are potentially useful for breeding high-glycogen oysters.

  • molecular characterization of pacific oyster Crassostrea Gigas irak4 gene and its role in myd88 dependent pathway
    Developmental and Comparative Immunology, 2017
    Co-Authors: Xueying Tang, Baoyu Huang, Linlin Zhang, Guofan Zhang
    Abstract:

    Interleukin-1 receptor-associated kinases (IRAKs) play important roles in MyD88-dependent TLR signaling, the crucial innate immune pathway in molluscs. In this study, we examined the full-length IRAK4 genetic sequence in the Pacific oyster (Crassostrea Gigas) by molecular cloning. Phylogenetic analysis revealed that CgIRAK4 is most closely related to Mytilus edulis, and forms a clade with other molluscs. CgIRAK4 transcripts are widely expressed in all tissues, with the highest expression observed in the hemocytes and gill. Moreover, CgIRAK4 is significantly upregulated after Oyster herpesvirus-1 microvariant (OsHV-1 μvar), Vibrio alginolyticus, and poly I:C challenge. Yeast two-hybrid and co-immunoprecipitation assays reveal that the CgIRAK4 death domain is necessary to mediate interaction between CgIRAK4 and two CgMyD88 isoforms. In addition, CgIRAK4 overexpression cannot induce NF-κB transcriptional activity, but blocks that induced by CgMyD88 in HEK293T cells. These findings elucidate the mechanisms of MyD88-dependent TLR signaling in molluscs, and the differences in IRAK-mediated pathway activation between invertebrates and vertebrates.

  • genome wide and single base resolution dna methylomes of the pacific oyster Crassostrea Gigas provide insight into the evolution of invertebrate cpg methylation
    BMC Genomics, 2014
    Co-Authors: Xiaotong Wang, Jie Meng, Ximing Guo, Linlin Zhang, Jinmin Lian, Lijun Jin, Huimin Cai, Huayong Que, Xiaodong Fang, Guofan Zhang
    Abstract:

    Background Studies of DNA methylomes in a wide range of eukaryotes have revealed both conserved and divergent characteristics of DNA methylation among phylogenetic groups. However, data on invertebrates particularly molluscs are limited, which hinders our understanding of the evolution of DNA methylation in metazoa. The sequencing of the Pacific oyster Crassostrea Gigas genome provides an opportunity for genome-wide profiling of DNA methylation in this model mollusc.

  • genome and transcriptome analyses provide insight into the euryhaline adaptation mechanism of Crassostrea Gigas
    PLOS ONE, 2013
    Co-Authors: Jie Meng, Qihui Zhu, Linlin Zhang, Zhicai She, Baoyu Huang, Guofan Zhang
    Abstract:

    Background: The Pacific oyster, Crassostrea Gigas, has developed special mechanisms to regulate its osmotic balance to adapt to fluctuations of salinities in coastal zones. To understand the oyster's euryhaline adaptation, we analyzed salt stress effectors metabolism pathways under different salinities (salt 5, 10, 15, 20, 25, 30 and 40 for 7 days) using transcriptome data, physiology experiment and quantitative real-time PCR.

Xuelin Zhao - One of the best experts on this subject based on the ideXlab platform.

Chantal Mourton - One of the best experts on this subject based on the ideXlab platform.