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A. P. Soldatkin - One of the best experts on this subject based on the ideXlab platform.
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Development of a New Biosensor by Adsorption of Creatinine Deiminase on Monolayers of Micro- and Nanoscale Zeolites
Springer Proceedings in Physics, 2017Co-Authors: S. V. Marchenko, O. P. Soldatkin, I. S. Kucherenko, Berna Ozansoy Kasap, Burcu Akata Kurc, Sergei V. Dzyadevych, Ihor I. Piliponskiy, Olha O. Mamchur, A. P. SoldatkinAbstract:This work is dedicated to the development of Creatinine-sensitive biosensor consisting of pH-sensitive field-effect transistor (pH-FET) and Creatinine Deiminase (CD) immobilized with various types of zeolites, in particular, silicalite, zeolite beta (BEA) and nanobeta, and BEA zeolites, modified with gold nanoparticles and ions. For comparison, the traditional method of CD immobilization in saturated glutaraldehyde (GA) vapor was used. To modify pH-FET with zeolites, a monolayer method of deposition was applied. All basic analytical characteristics of the developed biosensors were compared: linear range of Creatinine determination, time of response and regeneration, minimum limit of detection, and response reproducibility within a single biosensor; the calibration curves were plotted. It is shown that the use of zeolites of different types as adsorbents in the development of Creatinine-sensitive biosensors resulted in a decrease of time of response and regeneration, an increase in sensitivity of the bioselective element to Creatinine, and improvement in reproducibility of preparation of various biosensors, as compared with the method of covalent cross-linking in GA vapor.
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Potentiometric Biosensor System Based on Recombinant Urease and Creatinine Deiminase for Urea and Creatinine Determination in Blood Dialysate and Serum
Electroanalysis, 2015Co-Authors: S. V. Marchenko, O. P. Soldatkin, I. S. Kucherenko, A. P. SoldatkinAbstract:A biosensor system for simultaneous determination of Creatinine and urea in blood serum and dialysate samples was developed. It consisted of Creatinine and urea biosensors based on a potentiometric transducers with two identical pH-sensitive field-effect transistors. In Creatinine biosensor, Creatinine Deiminase immobilized via photopolymerization in PVA/SbQ polymer on one transistor served as a biorecognition element, while bovine serum albumin in PVA/SbQ polymer placed on the second transistor was used for reference. The urea biosensor was created in the same way but recombinant urease was used instead of Creatinine Deiminase. The linear ranges of Creatinine and urea measurement were 0.02–2 mM and 0.5–15 mM, correspondingly, which allowed simultaneous determination of the metabolites. Response time of the biosensor system was 2–3 min; RSD of responses did not exceeded 5 %. The biosensors demonstrated absence of non-selective response towards components of blood dialysate and serum. Urea and Creatinine concentrations were determined in 20 samples of blood dialysate and serum. The results correlated well with traditional methods of analysis. Creatinine and urea biosensors were stable during five months of storage (during this time the responses decreased by about 10 %). The proposed biosensor system can be effectively used for analysis of serum samples and for hemodialysis control.
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Application of Creatinine-sensitive biosensor for hemodialysis control.
Biosensors & bioelectronics, 2012Co-Authors: O. A. Zinchenko, S. V. Marchenko, A. P. Soldatkin, T.a. Sergeyeva, A. L. Kukla, A.s. Pavlyuchenko, E.k. Krasyuk, Anna V. El'skayaAbstract:Abstract The highly sensitive and selective potentiometric biosensor for Creatinine determination has been developed by us earlier. In it, pH-sensitive field effect transistors were used as transducer and immobilized Creatinine Deiminase (EC 3.5.4.21)—as a biosensitive element. In the work presented, we optimized this biosensor for Creatinine analysis in real samples of dialysate in patients with renal failure. The optimized version of biosensor was applied for on-line monitoring of the level of Creatinine in the patient's dialysate fluid in the course of dialysis session. High correlation between the biosensor analysis and traditional Jaffe method was demonstrated.
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development of potentiometric Creatinine sensitive biosensor based on isfet and Creatinine Deiminase immobilised in pva sbq photopolymeric membrane
Materials Science and Engineering: C, 2002Co-Authors: A. P. Soldatkin, Jean Montoriol, William Sant, Claude Martelet, Nicole JaffrezicrenaultAbstract:Abstract A Creatinine-sensitive biosensor was developed using ion-sensitive field-effect transistors (ISFETs) as transducers and immobilised Creatinine Deiminase (CD) as bioselective element. CD was immobilised by UV photopolymerisation in poly(vinyl alcohol) containing styrylpyridinium (PVA/SbQ) membrane on the dielectric gate of the ISFET transducer. The developed ENFETs demonstrated a dependence of the sensor sensitivity on NaCl and buffer concentration. Minimal detection limit for Creatinine determination in a model solution containing 144 mM NaCl and 5% bovine serum albumin (BSA), pH 7.4, was about 20 μM. Biosensor responses were quite reproducible and stable during continuous work at least for 25 h, and the relative standard deviation of the sensor response was approximately 3% ( n =45, for Creatinine concentration of 0.1 mM). It was shown that Creatinine-sensitive ENFETs demonstrated excellent storage stability for more than 6 months when kept dry at 4–6 °C.
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Development of potentiometric Creatinine-sensitive biosensor based on ISFET and Creatinine Deiminase immobilised in PVA/SbQ photopolymeric membrane
Materials Science and Engineering: C, 2002Co-Authors: A. P. Soldatkin, Jean Montoriol, William Sant, Claude Martelet, Nicole Jaffrezic-renaultAbstract:Abstract A Creatinine-sensitive biosensor was developed using ion-sensitive field-effect transistors (ISFETs) as transducers and immobilised Creatinine Deiminase (CD) as bioselective element. CD was immobilised by UV photopolymerisation in poly(vinyl alcohol) containing styrylpyridinium (PVA/SbQ) membrane on the dielectric gate of the ISFET transducer. The developed ENFETs demonstrated a dependence of the sensor sensitivity on NaCl and buffer concentration. Minimal detection limit for Creatinine determination in a model solution containing 144 mM NaCl and 5% bovine serum albumin (BSA), pH 7.4, was about 20 μM. Biosensor responses were quite reproducible and stable during continuous work at least for 25 h, and the relative standard deviation of the sensor response was approximately 3% ( n =45, for Creatinine concentration of 0.1 mM). It was shown that Creatinine-sensitive ENFETs demonstrated excellent storage stability for more than 6 months when kept dry at 4–6 °C.
Mingliao Tsai - One of the best experts on this subject based on the ideXlab platform.
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highly sensitive amperometric Creatinine biosensor based on Creatinine Deiminase nafion nanostructured polyaniline composite sensing film prepared with cyclic voltammetry
Materials Chemistry and Physics, 2018Co-Authors: Yuhsuan Chang, Mingliao TsaiAbstract:Abstract Creatinine Deiminase (CD) immobilized on Nafion®-nanostructured polyaniline (nsPANi) composite film prepared by the cyclic voltammetry (CV) is used as sensing material to measuring the concentration of Creatinine in the aqueous solution. The effect of cycle number, scanning rate, temperature and the concentrations of hydrochloric acid and aniline for preparing composite sensing film on the sensitivity of amperometric Creatinine biosensor are systematically investigated. The surface morphologies and the properties of as-prepared Nafion®-nsPANi composite film are analyzed by FESEM and FTIR, respectively. The maximum sensitivities for monitoring NH4+ based on Nafion®-nsPANi composite film are obtained to be 1647 and 376 μA mM−1 in the concentration ranges of 0.005–0.1 and 0.1–0.4 mM, respectively. By immobilizing CD enzyme on the Nafion®-nsPANi composite film the maximum sensitivity of amperometric Creatinine biosensor is 1300 μA mM−1 cm−2.
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Highly sensitive amperometric Creatinine biosensor based on Creatinine Deiminase/Nafion®-nanostructured polyaniline composite sensing film prepared with cyclic voltammetry
Materials Chemistry and Physics, 2018Co-Authors: Yuhsuan Chang, Mingliao TsaiAbstract:Abstract Creatinine Deiminase (CD) immobilized on Nafion®-nanostructured polyaniline (nsPANi) composite film prepared by the cyclic voltammetry (CV) is used as sensing material to measuring the concentration of Creatinine in the aqueous solution. The effect of cycle number, scanning rate, temperature and the concentrations of hydrochloric acid and aniline for preparing composite sensing film on the sensitivity of amperometric Creatinine biosensor are systematically investigated. The surface morphologies and the properties of as-prepared Nafion®-nsPANi composite film are analyzed by FESEM and FTIR, respectively. The maximum sensitivities for monitoring NH4+ based on Nafion®-nsPANi composite film are obtained to be 1647 and 376 μA mM−1 in the concentration ranges of 0.005–0.1 and 0.1–0.4 mM, respectively. By immobilizing CD enzyme on the Nafion®-nsPANi composite film the maximum sensitivity of amperometric Creatinine biosensor is 1300 μA mM−1 cm−2.
Yuhsuan Chang - One of the best experts on this subject based on the ideXlab platform.
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highly sensitive amperometric Creatinine biosensor based on Creatinine Deiminase nafion nanostructured polyaniline composite sensing film prepared with cyclic voltammetry
Materials Chemistry and Physics, 2018Co-Authors: Yuhsuan Chang, Mingliao TsaiAbstract:Abstract Creatinine Deiminase (CD) immobilized on Nafion®-nanostructured polyaniline (nsPANi) composite film prepared by the cyclic voltammetry (CV) is used as sensing material to measuring the concentration of Creatinine in the aqueous solution. The effect of cycle number, scanning rate, temperature and the concentrations of hydrochloric acid and aniline for preparing composite sensing film on the sensitivity of amperometric Creatinine biosensor are systematically investigated. The surface morphologies and the properties of as-prepared Nafion®-nsPANi composite film are analyzed by FESEM and FTIR, respectively. The maximum sensitivities for monitoring NH4+ based on Nafion®-nsPANi composite film are obtained to be 1647 and 376 μA mM−1 in the concentration ranges of 0.005–0.1 and 0.1–0.4 mM, respectively. By immobilizing CD enzyme on the Nafion®-nsPANi composite film the maximum sensitivity of amperometric Creatinine biosensor is 1300 μA mM−1 cm−2.
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Highly sensitive amperometric Creatinine biosensor based on Creatinine Deiminase/Nafion®-nanostructured polyaniline composite sensing film prepared with cyclic voltammetry
Materials Chemistry and Physics, 2018Co-Authors: Yuhsuan Chang, Mingliao TsaiAbstract:Abstract Creatinine Deiminase (CD) immobilized on Nafion®-nanostructured polyaniline (nsPANi) composite film prepared by the cyclic voltammetry (CV) is used as sensing material to measuring the concentration of Creatinine in the aqueous solution. The effect of cycle number, scanning rate, temperature and the concentrations of hydrochloric acid and aniline for preparing composite sensing film on the sensitivity of amperometric Creatinine biosensor are systematically investigated. The surface morphologies and the properties of as-prepared Nafion®-nsPANi composite film are analyzed by FESEM and FTIR, respectively. The maximum sensitivities for monitoring NH4+ based on Nafion®-nsPANi composite film are obtained to be 1647 and 376 μA mM−1 in the concentration ranges of 0.005–0.1 and 0.1–0.4 mM, respectively. By immobilizing CD enzyme on the Nafion®-nsPANi composite film the maximum sensitivity of amperometric Creatinine biosensor is 1300 μA mM−1 cm−2.
Nicole Jaffrezic-renault - One of the best experts on this subject based on the ideXlab platform.
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Creatinine sensitive biosensor based on ISFETs and Creatinine Deiminase immobilised in BSA membrane.
Talanta, 2002Co-Authors: Alexey P. Soldatkin, Jean Montoriol, William Sant, Claude Martelet, Nicole Jaffrezic-renaultAbstract:A Creatinine sensitive biosensor based on ion sensitive field-effect transistors (ISFETs) with immobilised Creatinine Deiminase (CD) is developed. CD is immobilised on the transducer surface by classical cross-linking with bovine serum albumin (BSA) in a glutaraldehyde (GA) vapour. The linear dynamic ranges of biosensors are between 0 and 5 mM Creatinine concentration, and the sensor sensitivity depends on the sample buffer concentration. Minimal detection limit for Creatinine determination in model solution with 144 mM NaCl and 5% BSA, pH 7.4, is about 10 muM. Biosensor responses are reproducible and stable during continuous work at least for 8 h, and the relative standard deviation of sensor response is approximately 3% (n=48, for Creatinine concentrations of 0.2 and 0.6 mM). An investigation about storage stability of Creatinine sensitive ENFETs kept in dry at 4-6 degrees C shows that biosensors demonstrate an excellent storage stability for at least 6 months and more. Moreover, Creatinine sensitive enzymatic field-effect transistors (ENFETs), demonstrating very good performances, are very selective and specific and well suitable for hemodialysis monitoring.
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Development of potentiometric Creatinine-sensitive biosensor based on ISFET and Creatinine Deiminase immobilised in PVA/SbQ photopolymeric membrane
Materials Science and Engineering: C, 2002Co-Authors: A. P. Soldatkin, Jean Montoriol, William Sant, Claude Martelet, Nicole Jaffrezic-renaultAbstract:Abstract A Creatinine-sensitive biosensor was developed using ion-sensitive field-effect transistors (ISFETs) as transducers and immobilised Creatinine Deiminase (CD) as bioselective element. CD was immobilised by UV photopolymerisation in poly(vinyl alcohol) containing styrylpyridinium (PVA/SbQ) membrane on the dielectric gate of the ISFET transducer. The developed ENFETs demonstrated a dependence of the sensor sensitivity on NaCl and buffer concentration. Minimal detection limit for Creatinine determination in a model solution containing 144 mM NaCl and 5% bovine serum albumin (BSA), pH 7.4, was about 20 μM. Biosensor responses were quite reproducible and stable during continuous work at least for 25 h, and the relative standard deviation of the sensor response was approximately 3% ( n =45, for Creatinine concentration of 0.1 mM). It was shown that Creatinine-sensitive ENFETs demonstrated excellent storage stability for more than 6 months when kept dry at 4–6 °C.
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Tailoring enzymatic membranes for ENFETs for dialysis monitoring
TRANSDUCERS '03. 12th International Conference on Solid-State Sensors Actuators and Microsystems. Digest of Technical Papers (Cat. No.03TH8664), 1Co-Authors: Nicole Jaffrezic-renault, Alexey P. Soldatkin, Claude Martelet, Pierre Temple-boyer, W. Sant, M.l. Pourciel, P. Montoril, A. Montiel-costesAbstract:We report here, for the first time, the tailoring of enzymatic membranes (enzyme origin, type of photocurable polymer, formulation of the membrane, conditions of deposition) in order to obtain ENFETs with specifications adapted to dialysis monitoring through direct detection of Creatinine and urea in plasmatic liquid. Creatinine Deiminase was immobilised in a PVA/SbQ membrane on the dielectric gate of a pH-sensitive ISFET, this membrane being deposited at the end of the microtechnological process and then processed using photolithography. Detection limit obtained in a model solution was about 20 /spl mu/M. A novel urea biosensor based on immobilised recombinant urease as sensitive element and ion sensitive field effect transistor (ISFET) as transducer was developed. Recombinant urease from E.coli with an increase Km was photoimmobilised in PVA/SbQ membrane and has demonstrated quite good performance as biosensitive element. The working characteristics of the ENFET based on such a bioselective element were studied in model buffer solutions. This biosensor demonstrated an extended dynamic range up to 80 mM, a quite good reproducibility and a high stability.
Yasuhiko Kato - One of the best experts on this subject based on the ideXlab platform.
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A Multifunctional Flow‐injection Biosensor for the Simultaneous Determination of Ammonia, Creatinine, and Urea
Annals of the New York Academy of Sciences, 1995Co-Authors: Chang Sheng Rui, Kenji Sonomoto, I.-ogawa Hiroaki, Yasuhiko KatoAbstract:A strategy for the multifunctionalization of the FIA biosensor was developed. The described multifunctional FIA system offers a fast and simple method for the simultaneous determination of ammonia, Creatinine, and urea. The hydrolysis of Creatinine by Creatinine Deiminase (CRDI) or of urea by urease forms ammonia, which is amperometrically detected by an oxygen electrode, based on an enzyme conversion system, glutamate dehydrogenase (GLDH)/glutamate oxidase (GLOD). The split of the stream into three after sample injection and confluence before the GLDH reactor resulted in a three-channel system, into which were set three parallel columns, respectively, filled with immobilized CRDI, urease, and CPG. A triple-peak recording was obtained by putting two delay coils at the channels involving CRDI and urease. Thus the interfering of the endogenous ammonia on the Creatinine and urea assay is simultaneously compensated. Furthermore, the problem of great difference in concentration between urea and the other two components is resolved by taking advantage of the differentiated dilution effect for each channel caused from the split-stream, flow-injection system. Linear calibration ranges for ammonia, Creatinine, and urea were 0.1-5, 0.2-10, and 2-40 mM, respectively. One run was finished within 5 minutes, and the system was reproducibility good (3 to 5%). The results of the urine assay obtained by the present method will be described in the near future.
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Amperometric flow-injection analysis of Creatinine based on immobilized Creatinine Deiminase, leucine dehydrogenase and L-amino acid oxidase.
Biosensors & bioelectronics, 1994Co-Authors: Chang Sheng Rui, Yasuhiko Kato, Kenji SonomotoAbstract:Leucine dehydrogenase/L-amino acid oxidase was proposed as an enzymatic conversion system for ammonia and its application to amperometric assay of Creatinine was investigated. Ammonia formed by Creatinine Deiminase catalyzed hydrolysis of Creatinine was converted to L-leucine by leucine dehydrogenase, and the oxidation of L-leucine by L-amino acid oxidase was detected with an oxygen electrode. Two approaches were proposed to overcome the problem of endogenous ammonia and L-amino acids. The first was using glutamate dehydrogenase prereactor to remove endogenous ammonia; endogenous L-amino acids were corrected by a separate run. In the second approach, endogenous ammonia and L-amino acids were simultaneously compensated with a two-channel system. It resulted in double peak recording that the flow was split and rejoined between the two ends of Creatinine Deiminase reactor and a delay coil and a reference column were properly set at one of the two-channels. One gave the sum response of all responsible compounds, the other that of endogenous interferences except Creatinine. Both approaches were applied to Creatinine assay in urine and the results showed a good agreement with those obtained from the Jaffe method.
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Multifunctional Flow-injection Biosensor for the Simultaneous Measurement of Creatinine, Glucose, and Urea.
Bioscience biotechnology and biochemistry, 1993Co-Authors: Chang Sheng Rui, Kenji Sonomoto, Hiroaki I. Ogawa, Yasuhiko KatoAbstract:An amperometric flow-injection biosensor system was developed for the simultaneous measurement of Creatinine, glucose, and urea, with a single sample injection and one detector. The principle for the amperometric detection of urea and Creatinine was based on coupled reactions of three sequentially aligned enzyme reactors, urease or Creatinine Deiminase/glutamate dehydrogenase/L-glutamate oxidase. Inclusion of a split point and two confluence points between the injector and detector (oxygen electrode) resulted in a flow-injection system composed of three channels. Triple peak recording was obtained by putting two delay coils of different lengths at the channels involving urease and glucose oxidase. The system gave linear calibrations for Creatinine, glucose, and urea in the ranges of 0.2–5, 0.2–10, and 0.5–20mM, respectively. The assay procedure was simple and one run was completed within 4 min. The system was reproducible within 5–8% of relative standard deviation.
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A Flow-Injection Biosensor System for the Amperometric Determination of Creatinine: Simultaneous Compensation of Endogenous Interferents
Analytical biochemistry, 1993Co-Authors: Chang Sheng Rui, Kenji Sonomoto, Hiroaki I. Ogawa, Yasuhiko KatoAbstract:Abstract A flow-injection biosensor system was developed for the amperometric determination of Creatinine based on coupled reactions of three sequentially aligned enzyme reactors, Creatinine Deiminase, glutamate dehydrogenase, and glutamate oxidase, using an oxygen electrode as the detector. To overcome the problem of endogenous ammonia and glutamate, the flow was split into two channels after the injector and rejoined before the glutamate dehydrogenase reactor. Double peak recording was obtained by setting a delay coil and a reference column in one of the two channels. The first peak gave the sum response of Creatinine, endogenous ammonia, and glutamate, and the second that of endogenous ammonia and glutamate. By this method compensation for endogenous ammonia and glutamate, as well as for interfering ascorbic acid, was achieved simultaneously. The system gave linear calibrations up to 2 mM for the first peak and 3 mM for the second one. The lower detection limits were 0.1 and 0.02 mM for 35- and 100-μl injection of sample, respectively. One run was completed within 2 min. The system showed good reproducibility ( 1300 runs). The assay results of Creatinine in urine showed good correlation with those obtained from the chemical method of Jaffe.
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Amperometric assay of Creatinine in urine by flow injection analysis based on conjugated reactions of immobilized enzymes. Simultaneous compensation of endogenous ammonia.
Annals of the New York Academy of Sciences, 1992Co-Authors: Chang Sheng Rui, Kenji Sonomoto, Hiroaki I. Ogawa, Yasuhiko KatoAbstract:A flow-injection analysis biosensor system was developed for the amperometric assay of Creatinine based on coupled reactions of three immobilized enzymes, using an oxygen electrode as the detection device. The ammonia produced by Creatinine Deiminase-catalyzed hydrolysis of Creatinine was further converted into L-glutamate with two sequentially aligned enzyme reactors: glutamate dehydrogenase and glutamate oxidase. Endogenous ammonia was simultaneously compensated with a double peak recording system, where the flow was split after sample injection and rejoined before the glutamate dehydrogenase reactor. The system gave linear calibration in a range of 0.1-2.0 mM for Creatinine and the first peak of ammonia, and 0.1-3.0 mM for the second peak of ammonia. One run was completed within two minutes. The system can be readily applied to the assay of Creatinine in urine and showed good correlation with that from the currently used Jaffe method.