The Experts below are selected from a list of 14436 Experts worldwide ranked by ideXlab platform
Martin John Woodward - One of the best experts on this subject based on the ideXlab platform.
-
DETECTION OF Cryptosporidium-parvum USING A SPECIFIC POLYMERASE CHAIN-REACTION
Veterinary parasitology, 1993Co-Authors: Katherine Anne Webster, Jonothan D.e. Pow, M. Giles, Janet Catchpole, Martin John WoodwardAbstract:The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys.
Huw V. Smith - One of the best experts on this subject based on the ideXlab platform.
-
Viability of Cryptosporidium parvum Oocysts: Assessment by the Dye Permeability Assay
Applied and environmental microbiology, 1998Co-Authors: Lucy J. Robertson, A. T. Campbell, Huw V. SmithAbstract:The paper by Jenkins et al. ([6][1]) provides interesting data on Cryptosporidium parvum oocyst permeability and survival. The vital dye assay ([4][2]) relies upon oocyst permeability and exhibits limitations, especially in assessment of disinfectant efficacy ([3][3]). However, it is rewarding to
-
Effects of Preservatives on Viability of Cryptosporidium parvum Oocysts.
Applied and environmental microbiology, 1993Co-Authors: A. T. Campbell, Lucy J. Robertson, Huw V. SmithAbstract:Potassium dichromate and formalin reduced the viability of Cryptosporidium parvum oocysts as assessed by inclusion or exclusion of 4′,6-diamidino-2-phenylindole (DAPI) and propidium iodide (PI) and excystation. Some formalin-treated oocysts containing dead sporozoites excluded PI; that this fluorogenic assay relies not solely upon exclusion of PI but also upon highlighting of sporozoite nuclei by DAPI is reiterated.
-
In vitro excystation of Cryptosporidium parvum.
Parasitology, 1993Co-Authors: Lucy J. Robertson, A. T. Campbell, Huw V. SmithAbstract:Protocols for in vitro excystation of oocysts of Cryptosporidium parvum , including different chemical pre-incubation steps, were compared to examine some of the biochemical triggers involved in excystation and to define an in vitro excystation protocol of a reproducibly high efficiency. Pre-incubation steps which increased the permeability of the oocysts were found to enhance excystation dynamics and pre-treatment of oocysts with saliva was found to decrease the permeability and reduce excystation. Although excystation was maximal after incubation for 4 h, sporozoites tended to lyse over this period, and maximum sporozoite recovery occurred after 30 min. The results obtained are discussed in relation to excystation protocols adopted by different research groups and a number of recommendations are given for in vitro excystation of C. parvum oocysts.
Xi Chen Zhang - One of the best experts on this subject based on the ideXlab platform.
-
A novel detection method of Cryptosporidium parvum infection in cattle based on Cryptosporidium parvum virus 1.
Acta biochimica et biophysica Sinica, 2018Co-Authors: Lixin Tai, Peng Tao Gong, Jigang Yin, Nan Zhang, Ju Yang, Zhengtao Yang, Xi Chen ZhangAbstract:Cryptosporidium parvum is an important zoonotic parasite that causes significant economic loss in the animal husbandry industry, especially the cattle industry. As there is no specific vaccine or drug against Cryptosporidium, a rapid and accurate method for the detection of C. parvum is of great significance. In this study, colloidal gold strips were developed based on Cryptosporidium parvum virus 1 (CSpV1) for the detection of C. parvum infection in cattle fecal samples. The colloidal gold solution was prepared by reducing trisodium citrate and the CSpV1 #5 monoclonal antibody was labeled with colloidal gold. A polyclonal antibody against the CSpV1 capsid protein and an anti-mouse IgG antibody were coated on the colloidal gold strips for use in the test and control lines, respectively. Our results showed that the detection sensitivity in fecal samples was up to a 1:64 dilution. There was no cross-reaction with Cryptosporidium andersoni or Giardia in the fecal samples. The different preservation conditions (room temperature, 4°C, and 37°C) and preservation time (7, 30, 60, and 90 days) were analyzed. The data showed that the strips could be preserved for 90 days at 4°C and for 60 days at room temperature or 37°C. The colloidal gold strips were used to detect the samples of 120 clinical fecal in Changchun, China. The results indicated that the rate of a positive test was 5% (6/120). This study provides a rapid and accurate method for detecting C. parvum infection in cattle and humans.
-
Prokaryotic expression and identification of S-dsRNA gene from Cryptosporidium parvum virus
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2011Co-Authors: Yu Mei Diao, Peng Tao Gong, Xiang Sheng Huang, Xi Chen ZhangAbstract:Objective To clone and express S-dsRNA gene of Cryptosporidium parvum virus, and investigate the reactogenicity of the recombinant. Methods Total RNA was extracted from Cryptosporidium parvum and S-dsRNA gene was amplified by RT-PCR. The PCR product was cloned into pET-28a(+) expression vector. The recombinant plasmid pET-28a(+)-S was transformed into E. coli BL21 (DE3) and induced with IPTG. The expression situation of recombinant protein was analyzed by SDS-PAGE. Its reactogenicity was examined by Western blotting analysis. Results pET-28a (+)-S was identified by PCR and double endonuclease digestion. SDS-PAGE result showed that the recombinant protein (M, 37,000) was expressed in the form of inclusion body. High level expression of recombinant protein was found at 1 mmol/L IPTG condition after incubation at 37 degrees C for 4 h and reached up to 72.6% of the total protein. The protein was recognized by the antisera from mice immunized with antigens from Cryptosporidium parvum oocysts. Conclusion The S-dsRNA gene of Cryptosporidium parvum virus has been expressed with adequate reactogenicity.
-
Cloning and sequencing of the gp23 gene encoding a surface antigen on sporozoites of Cryptosporidium parvum
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases, 2002Co-Authors: Xi Chen Zhang, Jigang Yin, Hong-sheng Ouyang, Jian-bao Chen, Ju YangAbstract:Objective To clone and sequence the gp23 gene encoding a surface antigen on the sporozoites of Cryptosporidium parvum . Methods Genomic DNA was isolated from oocysts of Cryptosporidium parvum . The gp23 gene was amplified by polymerase chain reaction (PCR) and cloned into pMD18 T vector and sequenced by the methods of dideoxy mediated chain termination. Results and Conclusion The gp23 gene was 345 bp in length and included an open reading frame encoding a protein of 114 amino acid residues. The homology of the nucleotide and amino acid sequences of the gp23 gene was 97.3% and 98.2% to that in the GenBank, respectively. The gp23 gene cloned contained 6 nucleotides more than that in the GenBank.
Katherine Anne Webster - One of the best experts on this subject based on the ideXlab platform.
-
DETECTION OF Cryptosporidium-parvum USING A SPECIFIC POLYMERASE CHAIN-REACTION
Veterinary parasitology, 1993Co-Authors: Katherine Anne Webster, Jonothan D.e. Pow, M. Giles, Janet Catchpole, Martin John WoodwardAbstract:The design and use of polymerase chain reaction primers and probes as reagents for the detection of Cryptosporidium parvum are described. Sensitive and specific amplification of a 329 base pair product was demonstrated by ethidium bromide staining and hybridisation of radiolabelled probes. These reagents have the potential for application to diagnostic samples, environmental monitoring and epidemiological surveys.
Robin Hirano - One of the best experts on this subject based on the ideXlab platform.
-
Risk assessment for Cryptosporidium parvum in reclaimed water
Water Research, 1999Co-Authors: Domènec Jolis, Paul Pitt, Robin HiranoAbstract:Abstract The risk of cryptosporidiosis associated with San Francisco's proposed water reclamation program is conservatively estimated using an exponential dose–response model based on human infectivity data for Cryptosporidium parvum. The observed C. parvum concentrations in filtered secondary effluent present less than a 1-in-10,000 annual risk of contracting the water-borne disease through regular exposure at parks and golf courses irrigated with tertiary reclaimed water in San Francisco.