The Experts below are selected from a list of 420 Experts worldwide ranked by ideXlab platform
J. Rees-george - One of the best experts on this subject based on the ideXlab platform.
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Species-specific PCR primers for Guignardia citricarpa and Guignardia mangiferae
New Zealand Plant Protection, 2006Co-Authors: K.r. Everett, J. Rees-georgeAbstract:The plant pathogen Guignardia citricarpa causes citrus black spot and is not considered to be present in New Zealand Speciesspecific polymerase chain reaction (PCR) primers were designed to identify G citricarpa and G mangiferae a closely related saprotroph that is present in New Zealand These PCR primers were tested against a range of other saprotrophic and pathogenic fungi viz Botrytis cinerea Botryosphaeria dothidea B parva Cladosporium sp Colletotrichum acutatum C gloeosporioides Cryptosporiopsis sp Epicoccum sp Nigrospora sp Penicillium sp Pestalotia sp Phialophora sp Phlyctema sp Phoma sp Phomopsis sp Stemphylium sp and Venturia inaequalis The primers JRGGc were specific to G citricarpa and JRGGm to G mangiferae A 226 bp product was amplified from G mangiferae DNA using JRGGm primers and a 501 bp product was amplified from G citricarpa DNA using JRGGc primers These primers thus distinguished G citricarpa from G mangiferae and can be used to rapidly identify incursions by citrus black spot
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Species-specific PCR primers for Guignardia citricarpa and Guignardia mangiferae
New Zealand Plant Protection, 2006Co-Authors: K.r. Everett, J. Rees-georgeAbstract:The plant pathogen Guignardia citricarpa causes citrus black spot and is not considered to be present in New Zealand Speciesspecific polymerase chain reaction (PCR) primers were designed to identify G citricarpa and G mangiferae a closely related saprotroph that is present in New Zealand These PCR primers were tested against a range of other saprotrophic and pathogenic fungi viz Botrytis cinerea Botryosphaeria dothidea B parva Cladosporium sp Colletotrichum acutatum C gloeosporioides Cryptosporiopsis sp Epicoccum sp Nigrospora sp Penicillium sp Pestalotia sp Phialophora sp Phlyctema sp Phoma sp Phomopsis sp Stemphylium sp and Venturia inaequalis The primers JRGGc were specific to G citricarpa and JRGGm to G mangiferae A 226 bp product was amplified from G mangiferae DNA using JRGGm primers and a 501 bp product was amplified from G citricarpa DNA using JRGGc primers These primers thus distinguished G citricarpa from G mangiferae and can be used to rapidly identify incursions by citrus black spot
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SPECIES-SPECIFICPCRPRIMERSFORGUIGNARDIA CITRICARPAANDGUIGNARDIA MANGIFERAE
2006Co-Authors: K.r. Everett, J. Rees-george, Mt AlbertAbstract:The plant pathogen Guignardia citricarpa causes citrus black spot and is not considered to be present in New Zealand. Species-specific polymerase chain reaction (PCR) primers were designed to identify G. citricarpa and G. mangiferae, a closely related saprotroph that is present in New Zealand. These PCR primers were tested against a range of other saprotrophic and pathogenic fungi viz. Botrytis cinerea, Botryosphaeria dothidea, B. parva, Cladosporium sp., Colletotrichum acutatum, C. gloeosporioides, Cryptosporiopsis sp., Epicoccum sp., Nigrospora sp., Penicillium sp., Pestalotia sp., Phialophora sp., Phlyctema sp., Phoma sp., Phomopsis sp., Stemphylium sp. and Venturia inaequalis. The primers JRGGc were specific to G. citricarpa, and JRGGm to G. mangiferae. A 226 bp product was amplified from G. mangiferae DNA using JRGGm primers, and a 501 bp product was amplified from G. citricarpa DNA using JRGGc primers. These primers thus distinguished G. citricarpa from G. mangiferae, and can be used to rapidly identify incursions by citrus black spot.
Gary G. Grove - One of the best experts on this subject based on the ideXlab platform.
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Comparative studies of Cryptosporiopsis curvispora and C. perennans. I. Morphology and pathogenic behavior.
Mycologia, 1993Co-Authors: Frank M. Dugan, Gary G. Grove, Jack D. RogersAbstract:Macroconidia of Cryptosporiopsis curvispora, cause of apple anthracnose, consistently displayed high curvature while those of C. perennans, cause of perennial canker, displayed low curvature when r...
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COMPARATIVE STUDIES OF Cryptosporiopsis CURVISPORA AND C. PERENNANS. II. CYTOLOGY AND VEGETATIVE COMPATIBILITY
Mycologia, 1993Co-Authors: Frank M. Dugan, Rodney G. Roberts, Gary G. GroveAbstract:Cryptosporiopsis curvispora, cause of apple anthracnose, and C. perennans, cause of perennial canker, were indistinguishable in gross cytology; macroconidia, microconidia, phialides and hyphal cells of each species were mostly uninucleate. Complementation of nitrate-nonutilizing mutants occurred within and between isolates of each species but not between species. Such complementations were common within and between isolates of C. perennans but rare within and between isolates of C. curvispora. The two fungi are considered to be distinct species. The teleomorph of C. perennans is transferred to Pezicula as P. perennans comb. nov.
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Perennial canker of apple: seasonal host susceptibility, spore production and perennation of Cryptosporiopsis perennans in infected fruit in eastern Washington
Plant Disease, 1992Co-Authors: Gary G. Grove, F. M. Dugan, R. J. BoalAbstract:Studies on the biology of Cryptosporiopsis perennans, causal agent of perennial canker and bull's-eye rot of apple, were conducted in orchards in eastern Washington during 1987-1990. Conidia were present on apple cankers throughout the year, with numbers peaking during November-February. The incubation and latent periods were 2-3 and 5-6 mo, respectively, on cankers resulting from artificial inoculation. In contrast to cankers resulting from natural inoculation, sporulation on these cankers was highest during the first year of canker growth and then declined. Trees were most susceptible to branch infection during the period from October to March (.)
K.r. Everett - One of the best experts on this subject based on the ideXlab platform.
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Neofabraea actinidiae in New Zealand kiwifruit orchards: current status and knowledge gaps.
New Zealand Plant Protection, 2019Co-Authors: J.l. Tyson, K.r. Everett, Michael A. Manning, Robert A. FullertonAbstract:Neofabraea actinidiae (syn. Cryptosporiopsis actinidiae) is a member of a suite of fungi associated with ‘ripe rots’ of kiwifruit. Although it has been recorded regularly from kiwifruit in New Zealand over the past 30-40 years, initially as ‘Cryptosporiopsis sp.’, there is a general lack of knowledge of this fungus. This paper provides a review of the current records and available literature on the taxonomy and biology of the organism, and assesses the knowledge gaps in the disease cycle and epidemiology of N. actinidiae in kiwifruit orchards. The conidia of the fungus are likely to be water borne, infect fruit during or near to flowering and remain latent until harvest and subsequent ripening. The source of inoculum remains unknown. This review may stimulate new research into this pathogen and give insights into potential control strategies.
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Species-specific PCR primers for Guignardia citricarpa and Guignardia mangiferae
New Zealand Plant Protection, 2006Co-Authors: K.r. Everett, J. Rees-georgeAbstract:The plant pathogen Guignardia citricarpa causes citrus black spot and is not considered to be present in New Zealand Speciesspecific polymerase chain reaction (PCR) primers were designed to identify G citricarpa and G mangiferae a closely related saprotroph that is present in New Zealand These PCR primers were tested against a range of other saprotrophic and pathogenic fungi viz Botrytis cinerea Botryosphaeria dothidea B parva Cladosporium sp Colletotrichum acutatum C gloeosporioides Cryptosporiopsis sp Epicoccum sp Nigrospora sp Penicillium sp Pestalotia sp Phialophora sp Phlyctema sp Phoma sp Phomopsis sp Stemphylium sp and Venturia inaequalis The primers JRGGc were specific to G citricarpa and JRGGm to G mangiferae A 226 bp product was amplified from G mangiferae DNA using JRGGm primers and a 501 bp product was amplified from G citricarpa DNA using JRGGc primers These primers thus distinguished G citricarpa from G mangiferae and can be used to rapidly identify incursions by citrus black spot
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Species-specific PCR primers for Guignardia citricarpa and Guignardia mangiferae
New Zealand Plant Protection, 2006Co-Authors: K.r. Everett, J. Rees-georgeAbstract:The plant pathogen Guignardia citricarpa causes citrus black spot and is not considered to be present in New Zealand Speciesspecific polymerase chain reaction (PCR) primers were designed to identify G citricarpa and G mangiferae a closely related saprotroph that is present in New Zealand These PCR primers were tested against a range of other saprotrophic and pathogenic fungi viz Botrytis cinerea Botryosphaeria dothidea B parva Cladosporium sp Colletotrichum acutatum C gloeosporioides Cryptosporiopsis sp Epicoccum sp Nigrospora sp Penicillium sp Pestalotia sp Phialophora sp Phlyctema sp Phoma sp Phomopsis sp Stemphylium sp and Venturia inaequalis The primers JRGGc were specific to G citricarpa and JRGGm to G mangiferae A 226 bp product was amplified from G mangiferae DNA using JRGGm primers and a 501 bp product was amplified from G citricarpa DNA using JRGGc primers These primers thus distinguished G citricarpa from G mangiferae and can be used to rapidly identify incursions by citrus black spot
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SPECIES-SPECIFICPCRPRIMERSFORGUIGNARDIA CITRICARPAANDGUIGNARDIA MANGIFERAE
2006Co-Authors: K.r. Everett, J. Rees-george, Mt AlbertAbstract:The plant pathogen Guignardia citricarpa causes citrus black spot and is not considered to be present in New Zealand. Species-specific polymerase chain reaction (PCR) primers were designed to identify G. citricarpa and G. mangiferae, a closely related saprotroph that is present in New Zealand. These PCR primers were tested against a range of other saprotrophic and pathogenic fungi viz. Botrytis cinerea, Botryosphaeria dothidea, B. parva, Cladosporium sp., Colletotrichum acutatum, C. gloeosporioides, Cryptosporiopsis sp., Epicoccum sp., Nigrospora sp., Penicillium sp., Pestalotia sp., Phialophora sp., Phlyctema sp., Phoma sp., Phomopsis sp., Stemphylium sp. and Venturia inaequalis. The primers JRGGc were specific to G. citricarpa, and JRGGm to G. mangiferae. A 226 bp product was amplified from G. mangiferae DNA using JRGGm primers, and a 501 bp product was amplified from G. citricarpa DNA using JRGGc primers. These primers thus distinguished G. citricarpa from G. mangiferae, and can be used to rapidly identify incursions by citrus black spot.
Sunil Kumar - One of the best experts on this subject based on the ideXlab platform.
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Isolation of three new metabolites and intervention of diazomethane led to separation of compound 1 & 2 from an endophytic fungus, Cryptosporiopsis sp. depicting cytotoxic activity
Medicinal Chemistry Research, 2017Co-Authors: Sunil Kumar, Masroor Qadri, Yedukondalu Nalli, Shashi Bhushan, Syed Riyaz-ul-hassan, Naresh K. Satti, Vivek Gupta, Asif AliAbstract:The discovery of three new natural products ( 1 , 4 , and 5 ), one semi-synthetic derivative ( 3 ) along with two known compounds ( 2 and 6 ) were isolated from an endophytic fungus Cryptosporiopsis sp. The structural elucidations of 1 – 6 were authenticated by one-dimensional and two-dimensional nuclear magnetic resonance, mass spectroscopy, and X-ray diffraction analysis. Herein, we intervention of diazomethane as tool that help in the crystallization and isolation of inseparable mixtures of compounds 1 and 2 . Compounds ( 1 – 6 ) were screened for cytotoxic activity against six cancer cell lines in which the 4-epi-ethisolide ( 2 ) exhibited moderate activity with IC_50 values 11 µM in HL-60, whereas the compound 3 lost its cytotoxic potentiality, but it displayed moderate antimicrobial activity. The result illustrates that the methylene moiety in 2 plays significant role in cytotoxic potential.
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isolation of three new metabolites and intervention of diazomethane led to separation of compound 1 2 from an endophytic fungus Cryptosporiopsis sp depicting cytotoxic activity
Medicinal Chemistry Research, 2017Co-Authors: Masroor Qadri, Yedukondalu Nalli, Sunil Kumar, Naresh K. Satti, Syed Riyazulhassan, Vivek K GuptaAbstract:The discovery of three new natural products (1, 4, and 5), one semi-synthetic derivative (3) along with two known compounds (2 and 6) were isolated from an endophytic fungus Cryptosporiopsis sp. The structural elucidations of 1–6 were authenticated by one-dimensional and two-dimensional nuclear magnetic resonance, mass spectroscopy, and X-ray diffraction analysis. Herein, we intervention of diazomethane as tool that help in the crystallization and isolation of inseparable mixtures of compounds 1 and 2. Compounds (1–6) were screened for cytotoxic activity against six cancer cell lines in which the 4-epi-ethisolide (2) exhibited moderate activity with IC50 values 11 µM in HL-60, whereas the compound 3 lost its cytotoxic potentiality, but it displayed moderate antimicrobial activity. The result illustrates that the methylene moiety in 2 plays significant role in cytotoxic potential.
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Bioactive metabolites from an endophytic Cryptosporiopsis sp. inhabiting Clidemia hirta.
Phytochemistry, 2013Co-Authors: Mahesh K. Zilla, Gary A. Strobel, Masroor Qadri, Anup Singh Pathania, Yedukondalu Nalli, Sunil Kumar, Santosh Kumar Guru, Shashi Bhushan, Sanjay K. Singh, Ram A. VishwakarmaAbstract:Abstract An endophytic Cryptosporiopsis sp. was isolated from Clidemia hirta and analyzed for its secondary metabolites that lead to the isolation of three bioactive molecules. The compounds were purified from the culture broth of the fungus and their structures were determined by spectroscopic methods as ( R )-5-hydroxy-2-methylchroman-4-one ( 1 ), 1-(2,6-dihydroxyphenyl)pentan-1-one ( 2 ) and ( Z )-1-(2-(2-butyryl-3-hydroxyphenoxy)-6-hydroxyphenyl)-3-hydroxybut-2-en-1-one ( 3 ). Compound 1 exhibited significant cytotoxic activity against the human leukemia cell line, HL-60 with an IC 50 of 4 μg/ml. This compound induced G2 arrest of the HL-60 cell cycle significantly. In addition, out of these compounds, 2 and 3 were active against several bacterial pathogens. Compound 2 was active against Bacillus cereus , Escherichia coli and Staphylococcus aureus with IC 50 values varying from 18 to 30 μg/ml, and compound 3 displayed activity against Pseudomonas fluorescens with an IC 50 value of 6 μg/ml. Compounds 2 and 3 are novel whereas compound 1 was reported earlier but the stereochemistry of its C-2 methyl is established for the first time.
Wei Wang - One of the best experts on this subject based on the ideXlab platform.
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Conidiomatal ultrastructure and cultural characteristics of root-inhabiting species of Cryptosporiopsis
Mycology, 2011Co-Authors: Wei WangAbstract:The conidiomatal differences among six root-inhabiting Cryptosporiopsis species were studied at an ultrastructral level and a dichotomous key was then provided. Cryptosporiopsis brunnea is delimited by its conidiomata having a distinct sterile excipular covering tissue without adhesive amorphous material and smooth to pitted macroconidia. Cryptosporiopsis radicicola produces only excipular covering conidioma-like tissue with adhesive amorphous material and setae. Synnematous conidiomata with abundant macroconidia dominate the colony of C. ericae. Cryptosporiopsis rhizophila is different in its globose to subglobose conidiomata, consisting of loosely aggregated vegetative hyphae developing macroconidial conidiophores. Cryptosporiopsis grisea, being the only teleomorph-connected species, differs from the others in its distinct columnar surface structures composed of entangling hyphae and rising well above the aerial mycelium, and unique platform-like hymenium consisting of tightly packed hyphal stroma inter...
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Endomembrane system of aspen root cells plays a key role in defense against a common fungal root endophyte, Cryptosporiopsis radicicola
Mycologia, 2009Co-Authors: Akihiko Tsuneda, Wei Wang, Ichiko Tsuneda, Randolph S. CurrahAbstract:The host-endophyte interaction between roots of aspen (Populus tremuloides) and Cryptosporiopsis radicicola was examined primarily by transmission electron microscopy. Hyphae growing on the exterior of the inoculated roots had a thick, electron-dense, adhesive sheath. At hyphal contact and penetration, host epidermal cells exhibited a series of defense responses (viz. formation of papillae and partition walls, general wall thickening and walling-off of internal hyphae). In papilla formation, loop-shaped, rough endoplasmic reticula (rER) gave rise to globose secretory vesicles that accumulated around and then fused to the developing papilla. Unlike papillae, general wall thickening was associated with the Golgi apparatus (GA) that produced cell wall materials; 1–3 layers of Golgi cisternae were in contact with or in the immediate proximity (mostly within 0–0.5 μ m) of and lying parallel to the host cell wall, where they budded out numerous subglobose vesicles that fused directly to the host cell wall and m...